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The map label for this gene is ybiW [H]

Identifier: 78222751

GI number: 78222751

Start: 1742442

End: 1745030

Strand: Reverse

Name: ybiW [H]

Synonym: Gmet_1539

Alternate gene names: 78222751

Gene position: 1745030-1742442 (Counterclockwise)

Preceding gene: 78222752

Following gene: 78222750

Centisome position: 43.65

GC content: 53.42

Gene sequence:

>2589_bases
ATGTCAACGGTTGCTGCATCAATTAAGTACAATGACAAGGTTGTCGATTTTCCGCTGGCAAATCAGGAAGAAAATGCAAT
AGCTGATGAAGTACTTCATGAAAATCTTGCCCGTCCCACCACGGAGAGGACAAAGAGACTCAAGGCACGGTGCCGCTGGA
AGCATGCCGCAGCGGGAGAATTTGTCGATGCGGAGGTTCGGGCCGGCATCGAGCGGATGCGCTTCATCACCGAAGCGCAC
AAGGCGAGCGCAGGGCAGCCGGAAGTGATCAGAAGAGCGTTGGGACTCGCGAACATATTGAACAAAAGCACGCTGGTCCT
GCAGCAGGACGAATTCATCATCGGCTACCACGCCGAAGACCCCGAGATGTTTCCGCTCTATCCTGAACTCGCCTATATGG
CGGTTCAGGACTACCTCATGAGTGACTACTCCCCCCAGCCCAAAGAAGAAGCTGCGGAGATCAACGAATACTGGAAGAAA
TACAGCATGCAGGCCAAGGGCGAGCGGTATTTCACCCAGGAAGAACTCCTGCAGATGTACCAGGTGAGCACCATGGAGGC
TCCGGGGTTCGCCACGGGCTACAACAGTATCAGCCCCCCTTACGAAACGGTGCTGCAGGACGGTCTGTTGAAACGGATTG
AAATGGCGCACGAGAAGATCGAGCATGCCAAAAGGGAAATGCAGAAGATTCCATGGGATGCAACAACCGGACTGGATTGG
ATAGCCAAGATCGATGTCTGGAAGGCAATGATCATCGCCGATGAAGCCGTGATCAACTGGGCCAGGCGCCACGCCCGTCT
CGCAAAAATTGTTGCCGAGAATTTCGAAACAAACCCTGCAAGAAAGGAAGAATTGCTGGAGATAGCTGAGATAAGCCACC
GCGTGCCGGCAGAGCCGTGCAAAGGCTTGAAAGATGCGTTCCAGGCCAAATGGTATACCTACCTGATCTGTCATGCCATA
GACCGCTATGCCAGCGGTTATGCCCAAAAAGAAGACGAGATGCTGGAGCCCTATTACAACATAAGCGTCAAGGAGAAATC
CTTCCAGCCCATGACCCACACCGATGTGGTGGAAATGGTTGAAATGGAGCGACTGAAGATCTCGGAACATGGCGCAGGGA
AGTCCAGAGCATACCGCGAAATCTTCCCCGGTTCCAACGACCTGTTCATTCTGACCATCGGCGGGACCAAGCCCGGTTAT
GTGGATGCCTGCAGTGACATGACCGATGCCATTCTCGAAGGTGCAAGGAACATCAGAACCACGGAACCGTCCATCGTGTT
CAGGTGGCATCCGGTCGGCCGCGAGAAAACCAAACGCCTTGTTTTCGAGTGCATCCGGGATGGCCTGGGGTACCCCTCAA
TCAAGCATGACGTCATTGGTACGGAACAACTGAAGTATTACAGCCAGTTCAGCAAGAATAACAATGGGGCGACCGATGAC
GAGGCCCATTACTGGGGCCTGGTGTTGTGCATGTCTCCCGGTGTATGTGGCAGGCGTAAGACCCATAAGACAAGGTCGGA
AGGTGGCGGCTCGATCTTCCCGGCCAAGATGATGGAAATCGTCCTTGCAGACGGGTTTGACTGGTCCTATTCGGGCATGC
AGCTGGGTCCGCACACTGGGGACCCTACGACATTCAAGACGTTTGAACAGCTGTGGGAAGCGTTCAGGAGCCAGTATGCC
TACGCGACGAGCAAGGTGATCCGTGCCAAGGACATCATGCGTTATTACGAATCGAAATTCCTGCAGATGCCTTTCGTCTC
CAGCATCGATGATGGCTGCATGGAGCTGGGCATCGACAGCATGGAACTTTCCGAGCAGCCGAACGGTTGGCATAACCCCA
TCACGACAGTCGTTGCCGCCAACTCCCTGGTTGCCATCAAGAAACTGATCTATGACGAGAAGAAGTACACGATGGCCCAG
CTCGTCACTGCTCTCCGCGCAAATTGGCACGGCTACGAGGATATGCGCCAGGATTTCCTGAATGCACCCAAATGGGGCAA
TGACGACGAATATGCCGATACCATCATCAAGAAATTTTACGAGGATATCATTGGCGGCGAGATGGCAAAGATCACCAATT
ATTCGGGTGGACCAGTACTTCCCGTAGGTCAGGCGGTCGGACTCTACATGGAAATCGGGTCACGGACCGGTCCAACCCCG
GATGGCCGTTTCGGTGGCGATGCCGGTGACGACGGGGGTATTTCTCCCTACATGGGGACGGACAAAAAAGGACCGACTGC
CGTACTGAAGTCCGTTTCCAAAGTGCAGAAAAACCAGAAAGCCAATCTGCTCAACCAGAGGCTTTCCGTGCCGATCATGC
GTAGCGTGCATGGTTTTACTATCTGGAAATCCTATATGGATGCCTGGGAGAAACTCAACATAGATCATGTCCAGTTCAAC
TGCGTGAGCACTGCAGAGATGAAGGCCGCTCAAAAGGAACCCGAAAAGCATCAGGATCTCATCGTCAGGGTTTCAGGCTT
CAGTGCCCGCTTTGTGGATATCCCGACCTATGGCCAAAACACCATCATCGCCAGAAACGAACAGGCCTTCGGTGCCGATG
ATCTTGAATACCTGAACACACAACTCTAA

Upstream 100 bases:

>100_bases
GCAAAGGGTAAATACTGGCTGTCCAAGCCGACCAGGAACGATTCTCCATCATGTAAAGCATTCAAAACAGCATAACAAAT
GATCAAACAGGGGGTTATTC

Downstream 100 bases:

>100_bases
CAAATACATCCACGTGGGGGCATTCCCGGCGGGATGTCTCCACGTGGCCCGGGACAGGAGGCAACAGTGAGCGCAACAAG
TAATGGCAACATGATGCACG

Product: formate C-acetyltransferase glycine radical:pyruvate formate-lyase, PFL

Products: NA

Alternate protein names: Pyruvate formate-lyase 3 [H]

Number of amino acids: Translated: 862; Mature: 861

Protein sequence:

>862_residues
MSTVAASIKYNDKVVDFPLANQEENAIADEVLHENLARPTTERTKRLKARCRWKHAAAGEFVDAEVRAGIERMRFITEAH
KASAGQPEVIRRALGLANILNKSTLVLQQDEFIIGYHAEDPEMFPLYPELAYMAVQDYLMSDYSPQPKEEAAEINEYWKK
YSMQAKGERYFTQEELLQMYQVSTMEAPGFATGYNSISPPYETVLQDGLLKRIEMAHEKIEHAKREMQKIPWDATTGLDW
IAKIDVWKAMIIADEAVINWARRHARLAKIVAENFETNPARKEELLEIAEISHRVPAEPCKGLKDAFQAKWYTYLICHAI
DRYASGYAQKEDEMLEPYYNISVKEKSFQPMTHTDVVEMVEMERLKISEHGAGKSRAYREIFPGSNDLFILTIGGTKPGY
VDACSDMTDAILEGARNIRTTEPSIVFRWHPVGREKTKRLVFECIRDGLGYPSIKHDVIGTEQLKYYSQFSKNNNGATDD
EAHYWGLVLCMSPGVCGRRKTHKTRSEGGGSIFPAKMMEIVLADGFDWSYSGMQLGPHTGDPTTFKTFEQLWEAFRSQYA
YATSKVIRAKDIMRYYESKFLQMPFVSSIDDGCMELGIDSMELSEQPNGWHNPITTVVAANSLVAIKKLIYDEKKYTMAQ
LVTALRANWHGYEDMRQDFLNAPKWGNDDEYADTIIKKFYEDIIGGEMAKITNYSGGPVLPVGQAVGLYMEIGSRTGPTP
DGRFGGDAGDDGGISPYMGTDKKGPTAVLKSVSKVQKNQKANLLNQRLSVPIMRSVHGFTIWKSYMDAWEKLNIDHVQFN
CVSTAEMKAAQKEPEKHQDLIVRVSGFSARFVDIPTYGQNTIIARNEQAFGADDLEYLNTQL

Sequences:

>Translated_862_residues
MSTVAASIKYNDKVVDFPLANQEENAIADEVLHENLARPTTERTKRLKARCRWKHAAAGEFVDAEVRAGIERMRFITEAH
KASAGQPEVIRRALGLANILNKSTLVLQQDEFIIGYHAEDPEMFPLYPELAYMAVQDYLMSDYSPQPKEEAAEINEYWKK
YSMQAKGERYFTQEELLQMYQVSTMEAPGFATGYNSISPPYETVLQDGLLKRIEMAHEKIEHAKREMQKIPWDATTGLDW
IAKIDVWKAMIIADEAVINWARRHARLAKIVAENFETNPARKEELLEIAEISHRVPAEPCKGLKDAFQAKWYTYLICHAI
DRYASGYAQKEDEMLEPYYNISVKEKSFQPMTHTDVVEMVEMERLKISEHGAGKSRAYREIFPGSNDLFILTIGGTKPGY
VDACSDMTDAILEGARNIRTTEPSIVFRWHPVGREKTKRLVFECIRDGLGYPSIKHDVIGTEQLKYYSQFSKNNNGATDD
EAHYWGLVLCMSPGVCGRRKTHKTRSEGGGSIFPAKMMEIVLADGFDWSYSGMQLGPHTGDPTTFKTFEQLWEAFRSQYA
YATSKVIRAKDIMRYYESKFLQMPFVSSIDDGCMELGIDSMELSEQPNGWHNPITTVVAANSLVAIKKLIYDEKKYTMAQ
LVTALRANWHGYEDMRQDFLNAPKWGNDDEYADTIIKKFYEDIIGGEMAKITNYSGGPVLPVGQAVGLYMEIGSRTGPTP
DGRFGGDAGDDGGISPYMGTDKKGPTAVLKSVSKVQKNQKANLLNQRLSVPIMRSVHGFTIWKSYMDAWEKLNIDHVQFN
CVSTAEMKAAQKEPEKHQDLIVRVSGFSARFVDIPTYGQNTIIARNEQAFGADDLEYLNTQL
>Mature_861_residues
STVAASIKYNDKVVDFPLANQEENAIADEVLHENLARPTTERTKRLKARCRWKHAAAGEFVDAEVRAGIERMRFITEAHK
ASAGQPEVIRRALGLANILNKSTLVLQQDEFIIGYHAEDPEMFPLYPELAYMAVQDYLMSDYSPQPKEEAAEINEYWKKY
SMQAKGERYFTQEELLQMYQVSTMEAPGFATGYNSISPPYETVLQDGLLKRIEMAHEKIEHAKREMQKIPWDATTGLDWI
AKIDVWKAMIIADEAVINWARRHARLAKIVAENFETNPARKEELLEIAEISHRVPAEPCKGLKDAFQAKWYTYLICHAID
RYASGYAQKEDEMLEPYYNISVKEKSFQPMTHTDVVEMVEMERLKISEHGAGKSRAYREIFPGSNDLFILTIGGTKPGYV
DACSDMTDAILEGARNIRTTEPSIVFRWHPVGREKTKRLVFECIRDGLGYPSIKHDVIGTEQLKYYSQFSKNNNGATDDE
AHYWGLVLCMSPGVCGRRKTHKTRSEGGGSIFPAKMMEIVLADGFDWSYSGMQLGPHTGDPTTFKTFEQLWEAFRSQYAY
ATSKVIRAKDIMRYYESKFLQMPFVSSIDDGCMELGIDSMELSEQPNGWHNPITTVVAANSLVAIKKLIYDEKKYTMAQL
VTALRANWHGYEDMRQDFLNAPKWGNDDEYADTIIKKFYEDIIGGEMAKITNYSGGPVLPVGQAVGLYMEIGSRTGPTPD
GRFGGDAGDDGGISPYMGTDKKGPTAVLKSVSKVQKNQKANLLNQRLSVPIMRSVHGFTIWKSYMDAWEKLNIDHVQFNC
VSTAEMKAAQKEPEKHQDLIVRVSGFSARFVDIPTYGQNTIIARNEQAFGADDLEYLNTQL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787044, Length=815, Percent_Identity=26.2576687116564, Blast_Score=219, Evalue=7e-58,
Organism=Escherichia coli, GI1790388, Length=798, Percent_Identity=24.812030075188, Blast_Score=213, Evalue=5e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 97614; Mature: 97483

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS51149 GLY_RADICAL_2 ; PS00133 CARBOXYPEPT_ZN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVAASIKYNDKVVDFPLANQEENAIADEVLHENLARPTTERTKRLKARCRWKHAAAGE
CCCEEEEEEECCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
FVDAEVRAGIERMRFITEAHKASAGQPEVIRRALGLANILNKSTLVLQQDEFIIGYHAED
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCCEEEEEECCC
PEMFPLYPELAYMAVQDYLMSDYSPQPKEEAAEINEYWKKYSMQAKGERYFTQEELLQMY
CCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
QVSTMEAPGFATGYNSISPPYETVLQDGLLKRIEMAHEKIEHAKREMQKIPWDATTGLDW
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
IAKIDVWKAMIIADEAVINWARRHARLAKIVAENFETNPARKEELLEIAEISHRVPAEPC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHH
KGLKDAFQAKWYTYLICHAIDRYASGYAQKEDEMLEPYYNISVKEKSFQPMTHTDVVEMV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHCCEEECCCCCCCCHHHHHHHH
EMERLKISEHGAGKSRAYREIFPGSNDLFILTIGGTKPGYVDACSDMTDAILEGARNIRT
HHHHHHHHCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCC
TEPSIVFRWHPVGREKTKRLVFECIRDGLGYPSIKHDVIGTEQLKYYSQFSKNNNGATDD
CCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHCCCCCCCCC
EAHYWGLVLCMSPGVCGRRKTHKTRSEGGGSIFPAKMMEIVLADGFDWSYSGMQLGPHTG
CCHHEEEEEEECCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEECCCCC
DPTTFKTFEQLWEAFRSQYAYATSKVIRAKDIMRYYESKFLQMPFVSSIDDGCMELGIDS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHCCCH
MELSEQPNGWHNPITTVVAANSLVAIKKLIYDEKKYTMAQLVTALRANWHGYEDMRQDFL
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NAPKWGNDDEYADTIIKKFYEDIIGGEMAKITNYSGGPVLPVGQAVGLYMEIGSRTGPTP
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHEECCCCCCCC
DGRFGGDAGDDGGISPYMGTDKKGPTAVLKSVSKVQKNQKANLLNQRLSVPIMRSVHGFT
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
IWKSYMDAWEKLNIDHVQFNCVSTAEMKAAQKEPEKHQDLIVRVSGFSARFVDIPTYGQN
HHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCHHCCCEEEEEECCCEEEEECCCCCCC
TIIARNEQAFGADDLEYLNTQL
EEEECCCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
STVAASIKYNDKVVDFPLANQEENAIADEVLHENLARPTTERTKRLKARCRWKHAAAGE
CCEEEEEEECCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
FVDAEVRAGIERMRFITEAHKASAGQPEVIRRALGLANILNKSTLVLQQDEFIIGYHAED
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEECCCEEEEEECCC
PEMFPLYPELAYMAVQDYLMSDYSPQPKEEAAEINEYWKKYSMQAKGERYFTQEELLQMY
CCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
QVSTMEAPGFATGYNSISPPYETVLQDGLLKRIEMAHEKIEHAKREMQKIPWDATTGLDW
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
IAKIDVWKAMIIADEAVINWARRHARLAKIVAENFETNPARKEELLEIAEISHRVPAEPC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHH
KGLKDAFQAKWYTYLICHAIDRYASGYAQKEDEMLEPYYNISVKEKSFQPMTHTDVVEMV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCHHCCEEECCCCCCCCHHHHHHHH
EMERLKISEHGAGKSRAYREIFPGSNDLFILTIGGTKPGYVDACSDMTDAILEGARNIRT
HHHHHHHHCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCC
TEPSIVFRWHPVGREKTKRLVFECIRDGLGYPSIKHDVIGTEQLKYYSQFSKNNNGATDD
CCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHCCCCCCCCC
EAHYWGLVLCMSPGVCGRRKTHKTRSEGGGSIFPAKMMEIVLADGFDWSYSGMQLGPHTG
CCHHEEEEEEECCCCCCCCHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEECCCCC
DPTTFKTFEQLWEAFRSQYAYATSKVIRAKDIMRYYESKFLQMPFVSSIDDGCMELGIDS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHCCCH
MELSEQPNGWHNPITTVVAANSLVAIKKLIYDEKKYTMAQLVTALRANWHGYEDMRQDFL
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
NAPKWGNDDEYADTIIKKFYEDIIGGEMAKITNYSGGPVLPVGQAVGLYMEIGSRTGPTP
CCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHEECCCCCCCC
DGRFGGDAGDDGGISPYMGTDKKGPTAVLKSVSKVQKNQKANLLNQRLSVPIMRSVHGFT
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
IWKSYMDAWEKLNIDHVQFNCVSTAEMKAAQKEPEKHQDLIVRVSGFSARFVDIPTYGQN
HHHHHHHHHHHCCCCEEEEEECCHHHHHHHHCCCHHCCCEEEEEECCCEEEEECCCCCCC
TIIARNEQAFGADDLEYLNTQL
EEEECCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]