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The map label for this gene is nhaA

Identifier: 78222307

GI number: 78222307

Start: 1212839

End: 1213987

Strand: Reverse

Name: nhaA

Synonym: Gmet_1089

Alternate gene names: 78222307

Gene position: 1213987-1212839 (Counterclockwise)

Preceding gene: 78222308

Following gene: 78222306

Centisome position: 30.37

GC content: 65.1

Gene sequence:

>1149_bases
ATGAGAAAACCGATCAATCTACTGAGGGAGTTCTCCGTCCCCCTCATCGCCGGAGTCATAACAGCCCTCGCTTGGGCAAA
CCTCGATCCCCGGGGGTATGATGCCCTTATCCATCAGCCTTCTTTCGGGGGGGTCAGCCTCCATTTCCTGGTCAATGAAC
TCTTCATGGTGCTCTTTTTCGGGATCGCAGCCGCCGAGATCACCCAGAGCTGCCTTCCCGGCGGGGACCTGAACCCGCCC
CGCAAGGCCGTCAACCCTCTGCTGGCGACCCTCGGCGGCGTCATCGGCCCAGTGCTCGTCTACCTGAGTCTCAATGCCGT
CATCGGTGACCCGACCCTGACCAAGGGGTGGGGAATCCCCACCGCAACCGACATTGCCCTGGCCTGGCTCGTGGCGCGGC
TGGTCTTTGGCGCCGGCCACCCGGCGGTATCGTTCCTCCTGCTCCTGGCCGTGGCTGACGACGCCATCGGGCTTGCCATC
ATCGCCGTCTTCTACCCCGATCCAGTCCACCCCACCGAGCCGATGTGGCTTTTCCTGACGGTGGCCGGCATCGTGGCCGC
CTACATCCTGCGGGGCGCCAGGGCGAAGAGCTACTGGCCCTATGTACTTGTGGGGGGAGGGCTCTCCTGGACCGGCCTGT
TCAAGGCCCACCTCCACCCGGCCCTGGCCCTCGTCTTCATCATCCCCTTCCTCCCCCATCCTCCGCGGGAATCGGCGCAC
CTGTTCGAGGAAAATCCCCGCGACACCTCCCCCCTCGCCCGTTTCGAGCACGACTGGAAGATCGTCGTCGACTTCGGCCT
CTTCCTCTTCGGCCTCGCCAACGCGGGAGTCCGCTTCTCCTCAGTGGGAACGGCCACCTGGCTCGTGCTGACAGCGCTCC
TGGTCGGCAAGACGGCAGGCATCTTAACCATGGGCTCACTGGGAAAGGCCCTCGGCTTCCCCCTCCCGGACAGGGTTGGG
TTCAAGGAACTGGCGTTGACGGGGCTGGTGGCCGGCATGGGGCTCACCGTAGCCCTCTTCGTGGCGGGGGTCGCCTTTGT
GGATCCCGACATCGAGGGTGCGGCCAAGATGGGGGCGCTCCTCTCGGGGGGAGTACTCCCGGTGGCCGTGGCGCTCGGCA
GAATCCTGAAGGTACGGAGAATCCCCTGA

Upstream 100 bases:

>100_bases
GCAGCATAAATTTTGTCGCACCTCAGCCGGCGGTGTCGCCGCGGAGACAGGGCTCCTCCCCTACCCTCCCGGCACCGCCG
GCTCTCAGGAACAGGATTGC

Downstream 100 bases:

>100_bases
GCTGGAGAGACGTGTGCCTTGACATACAGAATCGCTTCATTATACTCGCCTAAAACATCCCCATCAAAAGAGCGGAGGGA
GCACCATGCATGACAGATCC

Product: Na+/H+ antiporter NhaA

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaA

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MRKPINLLREFSVPLIAGVITALAWANLDPRGYDALIHQPSFGGVSLHFLVNELFMVLFFGIAAAEITQSCLPGGDLNPP
RKAVNPLLATLGGVIGPVLVYLSLNAVIGDPTLTKGWGIPTATDIALAWLVARLVFGAGHPAVSFLLLLAVADDAIGLAI
IAVFYPDPVHPTEPMWLFLTVAGIVAAYILRGARAKSYWPYVLVGGGLSWTGLFKAHLHPALALVFIIPFLPHPPRESAH
LFEENPRDTSPLARFEHDWKIVVDFGLFLFGLANAGVRFSSVGTATWLVLTALLVGKTAGILTMGSLGKALGFPLPDRVG
FKELALTGLVAGMGLTVALFVAGVAFVDPDIEGAAKMGALLSGGVLPVAVALGRILKVRRIP

Sequences:

>Translated_382_residues
MRKPINLLREFSVPLIAGVITALAWANLDPRGYDALIHQPSFGGVSLHFLVNELFMVLFFGIAAAEITQSCLPGGDLNPP
RKAVNPLLATLGGVIGPVLVYLSLNAVIGDPTLTKGWGIPTATDIALAWLVARLVFGAGHPAVSFLLLLAVADDAIGLAI
IAVFYPDPVHPTEPMWLFLTVAGIVAAYILRGARAKSYWPYVLVGGGLSWTGLFKAHLHPALALVFIIPFLPHPPRESAH
LFEENPRDTSPLARFEHDWKIVVDFGLFLFGLANAGVRFSSVGTATWLVLTALLVGKTAGILTMGSLGKALGFPLPDRVG
FKELALTGLVAGMGLTVALFVAGVAFVDPDIEGAAKMGALLSGGVLPVAVALGRILKVRRIP
>Mature_382_residues
MRKPINLLREFSVPLIAGVITALAWANLDPRGYDALIHQPSFGGVSLHFLVNELFMVLFFGIAAAEITQSCLPGGDLNPP
RKAVNPLLATLGGVIGPVLVYLSLNAVIGDPTLTKGWGIPTATDIALAWLVARLVFGAGHPAVSFLLLLAVADDAIGLAI
IAVFYPDPVHPTEPMWLFLTVAGIVAAYILRGARAKSYWPYVLVGGGLSWTGLFKAHLHPALALVFIIPFLPHPPRESAH
LFEENPRDTSPLARFEHDWKIVVDFGLFLFGLANAGVRFSSVGTATWLVLTALLVGKTAGILTMGSLGKALGFPLPDRVG
FKELALTGLVAGMGLTVALFVAGVAFVDPDIEGAAKMGALLSGGVLPVAVALGRILKVRRIP

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons

COG id: COG3004

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaA Na(+)/H(+) (TC 2.A.33) antiporter family

Homologues:

Organism=Escherichia coli, GI1786201, Length=344, Percent_Identity=33.7209302325581, Blast_Score=116, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NHAA_GEOMG (Q39WP5)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384054.1
- ProteinModelPortal:   Q39WP5
- SMR:   Q39WP5
- STRING:   Q39WP5
- GeneID:   3741296
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1089
- NMPDR:   fig|269799.3.peg.1473
- eggNOG:   COG3004
- HOGENOM:   HBG665619
- OMA:   VFGAGHP
- PhylomeDB:   Q39WP5
- ProtClustDB:   CLSK828801
- BioCyc:   GMET269799:GMET_1089-MONOMER
- HAMAP:   MF_01844
- InterPro:   IPR023171
- InterPro:   IPR004670

Pfam domain/function: PF06965 Na_H_antiport_1

EC number: NA

Molecular weight: Translated: 40374; Mature: 40374

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x10862280)-; HASH(0x111110c0)-; HASH(0x11601db4)-; HASH(0x111499e4)-; HASH(0x10078008)-; HASH(0x10921094)-; HASH(0x10db8ed0)-; HASH(0x11897670)-; HASH(0xf901ca0)-; HASH(0x11789250)-; HASH(0xf7e5e0c)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKPINLLREFSVPLIAGVITALAWANLDPRGYDALIHQPSFGGVSLHFLVNELFMVLFF
CCCHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCHHEEECCCCCCCHHHHHHHHHHHHHHH
GIAAAEITQSCLPGGDLNPPRKAVNPLLATLGGVIGPVLVYLSLNAVIGDPTLTKGWGIP
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
TATDIALAWLVARLVFGAGHPAVSFLLLLAVADDAIGLAIIAVFYPDPVHPTEPMWLFLT
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHEEEEECCCCCCCCCHHHHHHH
VAGIVAAYILRGARAKSYWPYVLVGGGLSWTGLFKAHLHPALALVFIIPFLPHPPRESAH
HHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
LFEENPRDTSPLARFEHDWKIVVDFGLFLFGLANAGVRFSSVGTATWLVLTALLVGKTAG
HHCCCCCCCCCHHHHCCCCEEHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHCCCC
ILTMGSLGKALGFPLPDRVGFKELALTGLVAGMGLTVALFVAGVAFVDPDIEGAAKMGAL
CEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LSGGVLPVAVALGRILKVRRIP
HCCCHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure
MRKPINLLREFSVPLIAGVITALAWANLDPRGYDALIHQPSFGGVSLHFLVNELFMVLFF
CCCHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCHHEEECCCCCCCHHHHHHHHHHHHHHH
GIAAAEITQSCLPGGDLNPPRKAVNPLLATLGGVIGPVLVYLSLNAVIGDPTLTKGWGIP
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
TATDIALAWLVARLVFGAGHPAVSFLLLLAVADDAIGLAIIAVFYPDPVHPTEPMWLFLT
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHEEEEECCCCCCCCCHHHHHHH
VAGIVAAYILRGARAKSYWPYVLVGGGLSWTGLFKAHLHPALALVFIIPFLPHPPRESAH
HHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
LFEENPRDTSPLARFEHDWKIVVDFGLFLFGLANAGVRFSSVGTATWLVLTALLVGKTAG
HHCCCCCCCCCHHHHCCCCEEHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHCCCC
ILTMGSLGKALGFPLPDRVGFKELALTGLVAGMGLTVALFVAGVAFVDPDIEGAAKMGAL
CEEHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LSGGVLPVAVALGRILKVRRIP
HCCCHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + 2 Proton [Periplasm] = Na (I) [Periplasm] + 2 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA