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The map label for this gene is cheB1

Identifier: 78222293

GI number: 78222293

Start: 1196661

End: 1197752

Strand: Reverse

Name: cheB1

Synonym: Gmet_1075

Alternate gene names: 78222293

Gene position: 1197752-1196661 (Counterclockwise)

Preceding gene: 78222294

Following gene: 78222287

Centisome position: 29.96

GC content: 64.47

Gene sequence:

>1092_bases
ATGAGCACGCAGCCCATCAAAGTTCTCATCGTCGATGACTCGGCCCTCATCCGAAGCCTGCTGACGGACATCATCAACAG
CCAGACCGATATGGAGGTGGTGGGAGTGGCGCCCGACCCCCTGGTGGCCCGGGAAAAGATCAAGGCGCTCAACCCGGACG
TTCTGACCCTCGACGTGGAAATGCCCCGGATGGACGGGCTGGTCTTCCTGGAAAAGCTGATGCGCCTTCGCCCCATGCCG
GTGCTCATGGTCTCGTCACTCACCGAAAAAAGCTCATTCCTGACTCTTCGGGCACTGGAGCTGGGGGCCGTCGACTTCGT
CACCAAGCCGAAGATCGACATCAGCCGGGGCATGCAGGAATACGCCCGAGAGATCACCGACAAGCTCCGGGTCGCCGCCA
AAGCCAGGGTACGGCAGCCCCCCCACGTCCACCTCTCCGTGGAGCGAAAGAATACCGCCGACGCAGTGCTGCCGATGGAA
CACCGGACCTTCTCCTCTACCGAGAAGATCATCGTCATCGGCGCCTCCACCGGCGGCACCGAGGCCCTGAAATCGTTCCT
CGTCGCCATGCCCGCCGACTCTCCCGGCATCCTCATCACCCAGCACATGCCCGAGGCTTTCACCCGCACCTTTGCCCAGC
GACTGAACAGCCTCTGCAGGATCAGCGTCAAGGAGGCGGAGCACGGTGAACGAATCCTCCCGGGCCACGCCTATGTCGCT
CCGGGAAACCGGCACCTTCTCCTCGCCCGCAGCGGAGCCAACTACGTGGTGGAGCTCTCCGACGGCCCGCCGGTCAGCCG
CCACCGGCCGTCGGTGGACGTCCTCTTCCGTTCGGCAGCCAACCGCGCCGGCAGGAATGCCGTCGGCATCATCATGACCG
GCATGGGAGACGACGGCGCCGCCGGCATGCTGGAGATGCGCGAAGCCGGCGCCTACACCTTCGCTCAGGACGAAAAAAGC
TGTGTGGTCTTCGGGATGCCCAAAGAGGCCATAGCGCGAGGCGGGGTTGACGAGGTGGCGCCCCTGGGCGAGATGCCTCG
ACGACTCTTCGGCTGGCTAGAGTCGCAGGGGAACCGGGCCTTCAGGGTGTGA

Upstream 100 bases:

>100_bases
TCTACCCGCGCAAGGTCTACTTCGCCCCGGCAACCGGCCGTGCCTTTGTCAGGCAGATCAGGACCGGCAGCATCGACTCG
CTGTTTATCGGAGAATCGTC

Downstream 100 bases:

>100_bases
AGCAGATCAATATTCCCCAACGATCAGTTCGTCGAGATCCTTCCTATCGCCCGTGCGGGTGAATTCCGCCGGCCGCCCCA
CTGCCACTACCGCCATAAGC

Product: chemotaxis-specific methylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MSTQPIKVLIVDDSALIRSLLTDIINSQTDMEVVGVAPDPLVAREKIKALNPDVLTLDVEMPRMDGLVFLEKLMRLRPMP
VLMVSSLTEKSSFLTLRALELGAVDFVTKPKIDISRGMQEYAREITDKLRVAAKARVRQPPHVHLSVERKNTADAVLPME
HRTFSSTEKIIVIGASTGGTEALKSFLVAMPADSPGILITQHMPEAFTRTFAQRLNSLCRISVKEAEHGERILPGHAYVA
PGNRHLLLARSGANYVVELSDGPPVSRHRPSVDVLFRSAANRAGRNAVGIIMTGMGDDGAAGMLEMREAGAYTFAQDEKS
CVVFGMPKEAIARGGVDEVAPLGEMPRRLFGWLESQGNRAFRV

Sequences:

>Translated_363_residues
MSTQPIKVLIVDDSALIRSLLTDIINSQTDMEVVGVAPDPLVAREKIKALNPDVLTLDVEMPRMDGLVFLEKLMRLRPMP
VLMVSSLTEKSSFLTLRALELGAVDFVTKPKIDISRGMQEYAREITDKLRVAAKARVRQPPHVHLSVERKNTADAVLPME
HRTFSSTEKIIVIGASTGGTEALKSFLVAMPADSPGILITQHMPEAFTRTFAQRLNSLCRISVKEAEHGERILPGHAYVA
PGNRHLLLARSGANYVVELSDGPPVSRHRPSVDVLFRSAANRAGRNAVGIIMTGMGDDGAAGMLEMREAGAYTFAQDEKS
CVVFGMPKEAIARGGVDEVAPLGEMPRRLFGWLESQGNRAFRV
>Mature_362_residues
STQPIKVLIVDDSALIRSLLTDIINSQTDMEVVGVAPDPLVAREKIKALNPDVLTLDVEMPRMDGLVFLEKLMRLRPMPV
LMVSSLTEKSSFLTLRALELGAVDFVTKPKIDISRGMQEYAREITDKLRVAAKARVRQPPHVHLSVERKNTADAVLPMEH
RTFSSTEKIIVIGASTGGTEALKSFLVAMPADSPGILITQHMPEAFTRTFAQRLNSLCRISVKEAEHGERILPGHAYVAP
GNRHLLLARSGANYVVELSDGPPVSRHRPSVDVLFRSAANRAGRNAVGIIMTGMGDDGAAGMLEMREAGAYTFAQDEKSC
VVFGMPKEAIARGGVDEVAPLGEMPRRLFGWLESQGNRAFRV

Specific function: Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by CheR

COG id: COG2201

COG function: function code NT; Chemotaxis response regulator containing a CheY-like receiver domain and a methylesterase domain

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788192, Length=358, Percent_Identity=62.2905027932961, Blast_Score=442, Evalue=1e-125,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CHEB1_GEOMG (Q39WQ9)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384040.1
- ProteinModelPortal:   Q39WQ9
- SMR:   Q39WQ9
- STRING:   Q39WQ9
- GeneID:   3741282
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1075
- NMPDR:   fig|269799.3.peg.1459
- eggNOG:   COG2201
- HOGENOM:   HBG705324
- OMA:   FVTKPKL
- PhylomeDB:   Q39WQ9
- ProtClustDB:   PRK00742
- BioCyc:   GMET269799:GMET_1075-MONOMER
- GO:   GO:0005737
- GO:   GO:0006935
- HAMAP:   MF_00099
- InterPro:   IPR011006
- InterPro:   IPR008248
- InterPro:   IPR000673
- InterPro:   IPR001789
- Gene3D:   G3DSA:3.40.50.180
- PIRSF:   PIRSF000876
- SMART:   SM00448

Pfam domain/function: PF01339 CheB_methylest; PF00072 Response_reg; SSF52738 Chemotax_RR_pGlu_Me-esterase; SSF52172 CheY_like

EC number: =3.1.1.61

Molecular weight: Translated: 39687; Mature: 39555

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS50122 CHEB; PS50110 RESPONSE_REGULATORY

Important sites: ACT_SITE 176-176 ACT_SITE 202-202 ACT_SITE 298-298

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTQPIKVLIVDDSALIRSLLTDIINSQTDMEVVGVAPDPLVAREKIKALNPDVLTLDVE
CCCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEC
MPRMDGLVFLEKLMRLRPMPVLMVSSLTEKSSFLTLRALELGAVDFVTKPKIDISRGMQE
CCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEEEEECCEEECCCCCCHHHHHHHH
YAREITDKLRVAAKARVRQPPHVHLSVERKNTADAVLPMEHRTFSSTEKIIVIGASTGGT
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEECCCCCCCCCCCEEEEEECCCCCH
EALKSFLVAMPADSPGILITQHMPEAFTRTFAQRLNSLCRISVKEAEHGERILPGHAYVA
HHHHHHHEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHEEEEECCCCCCEECCCCEEEC
PGNRHLLLARSGANYVVELSDGPPVSRHRPSVDVLFRSAANRAGRNAVGIIMTGMGDDGA
CCCCEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCC
AGMLEMREAGAYTFAQDEKSCVVFGMPKEAIARGGVDEVAPLGEMPRRLFGWLESQGNRA
CCHHHHHHCCCEEEECCCCCEEEEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEE
FRV
ECC
>Mature Secondary Structure 
STQPIKVLIVDDSALIRSLLTDIINSQTDMEVVGVAPDPLVAREKIKALNPDVLTLDVE
CCCCEEEEEECCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEC
MPRMDGLVFLEKLMRLRPMPVLMVSSLTEKSSFLTLRALELGAVDFVTKPKIDISRGMQE
CCCCCHHHHHHHHHHHCCCCHHHHHHHCCCCCEEEEEEEEECCEEECCCCCCHHHHHHHH
YAREITDKLRVAAKARVRQPPHVHLSVERKNTADAVLPMEHRTFSSTEKIIVIGASTGGT
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEECCCCCCCCCCCEEEEEECCCCCH
EALKSFLVAMPADSPGILITQHMPEAFTRTFAQRLNSLCRISVKEAEHGERILPGHAYVA
HHHHHHHEECCCCCCCEEEECCCCHHHHHHHHHHHHHHHEEEEECCCCCCEECCCCEEEC
PGNRHLLLARSGANYVVELSDGPPVSRHRPSVDVLFRSAANRAGRNAVGIIMTGMGDDGA
CCCCEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCC
AGMLEMREAGAYTFAQDEKSCVVFGMPKEAIARGGVDEVAPLGEMPRRLFGWLESQGNRA
CCHHHHHHCCCEEEECCCCCEEEEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEE
FRV
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA