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The map label for this gene is fdnH [C]

Identifier: 78222278

GI number: 78222278

Start: 1180313

End: 1181146

Strand: Direct

Name: fdnH [C]

Synonym: Gmet_1060

Alternate gene names: 78222278

Gene position: 1180313-1181146 (Clockwise)

Preceding gene: 78222277

Following gene: 78222279

Centisome position: 29.53

GC content: 63.19

Gene sequence:

>834_bases
ATGAGCTCCTCGACCATCGACTACGGCAAGACCAAGACGTTCCTCATCGACACCACCAAGTGCACCGGCTGCCGCGGCTG
CCAGGTTGCCTGCAAGCAGTGGAACCAGCTGAAGGGTGAGAAGACCACGTTCTTCACCGGCGAAGGGTACCAGAACCCGC
CCCAGATGTCCGAATTCACCTTCACCCGGGTGAAGTTCAAGGACTACGAGAAGCACGGGCAGAACGAGTTCGCCTTCTAC
AAGGAGATGTGCATGCACTGCAACGACCCCGCATGCGCCTCCGTCTGTCCCGTCGGCGCCTTCCACAAAACGCCGGAAGG
GCCTGTCATCTACAACGCCAAGAAGTGCATCGGCTGCCGCTTTTGCATGGTGGCCTGCCCCTTCGGCGTTCCCAAGTACG
AGTGGAGCAAGGCGTTTCCCTTGGTGAAGAAGTGCACCGGCTGCTACAGCCGGGTTAAGAACGGCCTTCACCCCGCCTGC
GCCACCGCCTGCCCCACCGCCATCACCTACGGCAGCCGGGACGAGATGCTGAAAGAGGCGGAGAAGCGGATGGCCGCCAA
GCCCGACCGCTACTTCAAGGTGGTCTACGGCAAGGAGGAGGCCGGCGGCACCAGCGTCCTCTATCTGACCCAGCAGCCCC
TGGACGAACTGGGCTTCCGGCCGGTCACCAAGCGGCCGCTCCCTTCCTACACCTGGCAGGCCCTGCGGTTCGTGCCGGGG
ATCTTCCTCACCGTTGGCGGCACCCTGTCGCTGGTCACCTGGTTCCAGCACCGCAAGGACCGGGTGAGGCGCGAGGAAGA
GGAACGGAACAGCCGGAAGGAGGGGAACCAATGA

Upstream 100 bases:

>100_bases
ACAGCGGTAACGTCCTCACCCCCACCGTTGGGTGCGCCAACACCGGCATTCCGGAGTTCAAGGCGTTCCTCTGCAACATC
GAGAAAGGGGGTAAGCGGGC

Downstream 100 bases:

>100_bases
CCGCGGCACGCATTGTCATGAATGAAATCAAAGGGTACCATCGCTTCATCAAGTTTCTGATGGTCCTCGTGGGGGGGGCG
GCTTTGGCCTCCGCTGTCCG

Product: formate dehydrogenase beta subunit

Products: CO2; NADH

Alternate protein names: HMC operon ORF 2 [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MSSSTIDYGKTKTFLIDTTKCTGCRGCQVACKQWNQLKGEKTTFFTGEGYQNPPQMSEFTFTRVKFKDYEKHGQNEFAFY
KEMCMHCNDPACASVCPVGAFHKTPEGPVIYNAKKCIGCRFCMVACPFGVPKYEWSKAFPLVKKCTGCYSRVKNGLHPAC
ATACPTAITYGSRDEMLKEAEKRMAAKPDRYFKVVYGKEEAGGTSVLYLTQQPLDELGFRPVTKRPLPSYTWQALRFVPG
IFLTVGGTLSLVTWFQHRKDRVRREEEERNSRKEGNQ

Sequences:

>Translated_277_residues
MSSSTIDYGKTKTFLIDTTKCTGCRGCQVACKQWNQLKGEKTTFFTGEGYQNPPQMSEFTFTRVKFKDYEKHGQNEFAFY
KEMCMHCNDPACASVCPVGAFHKTPEGPVIYNAKKCIGCRFCMVACPFGVPKYEWSKAFPLVKKCTGCYSRVKNGLHPAC
ATACPTAITYGSRDEMLKEAEKRMAAKPDRYFKVVYGKEEAGGTSVLYLTQQPLDELGFRPVTKRPLPSYTWQALRFVPG
IFLTVGGTLSLVTWFQHRKDRVRREEEERNSRKEGNQ
>Mature_276_residues
SSSTIDYGKTKTFLIDTTKCTGCRGCQVACKQWNQLKGEKTTFFTGEGYQNPPQMSEFTFTRVKFKDYEKHGQNEFAFYK
EMCMHCNDPACASVCPVGAFHKTPEGPVIYNAKKCIGCRFCMVACPFGVPKYEWSKAFPLVKKCTGCYSRVKNGLHPACA
TACPTAITYGSRDEMLKEAEKRMAAKPDRYFKVVYGKEEAGGTSVLYLTQQPLDELGFRPVTKRPLPSYTWQALRFVPGI
FLTVGGTLSLVTWFQHRKDRVRREEEERNSRKEGNQ

Specific function: HMWC (high-molecular-weight cytochrome c precursor), ORF2, ORF3, ORF4, ORF5, ORF6 in the HMC operon form a transmembrane protein complex that allows electron flow from the periplasmic hydrogenase to the cytoplasmic enzymes that catalyze reduction of sulfa

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787749, Length=214, Percent_Identity=39.2523364485981, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1789370, Length=245, Percent_Identity=35.5102040816327, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1790326, Length=280, Percent_Identity=31.7857142857143, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1787122, Length=181, Percent_Identity=37.5690607734807, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1787872, Length=191, Percent_Identity=37.696335078534, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI226510944, Length=164, Percent_Identity=29.8780487804878, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI2367345, Length=190, Percent_Identity=30.5263157894737, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI2367245, Length=153, Percent_Identity=31.3725490196078, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1789079, Length=128, Percent_Identity=29.6875, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787740, Length=105, Percent_Identity=35.2380952380952, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787478, Length=99, Percent_Identity=35.3535353535354, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788811, Length=158, Percent_Identity=29.1139240506329, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: 1.2.1.2

Molecular weight: Translated: 31438; Mature: 31307

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
5.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
8.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSTIDYGKTKTFLIDTTKCTGCRGCQVACKQWNQLKGEKTTFFTGEGYQNPPQMSEFT
CCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEE
FTRVKFKDYEKHGQNEFAFYKEMCMHCNDPACASVCPVGAFHKTPEGPVIYNAKKCIGCR
EEEEEEHHHHHCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCEEECHHHHHHHH
FCMVACPFGVPKYEWSKAFPLVKKCTGCYSRVKNGLHPACATACPTAITYGSRDEMLKEA
HEEHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCCCHHHHHHCCHHHCCCCHHHHHHHH
EKRMAAKPDRYFKVVYGKEEAGGTSVLYLTQQPLDELGFRPVTKRPLPSYTWQALRFVPG
HHHHHCCCCCEEEEEECCCCCCCCEEEEEECCCHHHHCCCCCCCCCCCHHHHHHHHHHCH
IFLTVGGTLSLVTWFQHRKDRVRREEEERNSRKEGNQ
HEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SSSTIDYGKTKTFLIDTTKCTGCRGCQVACKQWNQLKGEKTTFFTGEGYQNPPQMSEFT
CCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEE
FTRVKFKDYEKHGQNEFAFYKEMCMHCNDPACASVCPVGAFHKTPEGPVIYNAKKCIGCR
EEEEEEHHHHHCCCCHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCCEEECHHHHHHHH
FCMVACPFGVPKYEWSKAFPLVKKCTGCYSRVKNGLHPACATACPTAITYGSRDEMLKEA
HEEHCCCCCCCCCCHHHCCHHHHHHHHHHHHHHCCCCHHHHHHCCHHHCCCCHHHHHHHH
EKRMAAKPDRYFKVVYGKEEAGGTSVLYLTQQPLDELGFRPVTKRPLPSYTWQALRFVPG
HHHHHCCCCCEEEEEECCCCCCCCEEEEEECCCHHHHCCCCCCCCCCCHHHHHHHHHHCH
IFLTVGGTLSLVTWFQHRKDRVRREEEERNSRKEGNQ
HEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8335628 [H]