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The map label for this gene is zraR [H]

Identifier: 78222276

GI number: 78222276

Start: 1175618

End: 1177006

Strand: Reverse

Name: zraR [H]

Synonym: Gmet_1058

Alternate gene names: 78222276

Gene position: 1177006-1175618 (Counterclockwise)

Preceding gene: 78222287

Following gene: 78222275

Centisome position: 29.44

GC content: 65.23

Gene sequence:

>1389_bases
ATGAAAGCACGCATTCTCATTTGCGACGACGAGGAAGGGATCAGGCGCTACCTCCAGAAGATGTTCCTGGCCAAGGAGTA
CGATGTGGAAACCTTTGCCGACGGAACGAGCCTTCTGGCACGTATTGAGGGGGGAACGGAGGGTGATGCCGACATCCTCC
TTCAGGACGTGCGGATGCCGGACATGGACGGGATCGAGGTGCTGAAGCGGGTGAAGAAGCTCCGGCCGGCCATGCCGGTG
GTGGTGATGACCGCCTTCGGCACCATCGACGCGGCCGTGGAGGCGATCAAGCTGGGGGCCTACGACTACGTCACCAAGCC
GTTCCCCAAGGAGAAGATCCTCGGCCTCATGGAGAACGCCCTCCAACTGGACCTCCTCATGAAGGAGAACCGGGTGCTGA
AGGAGGAGCTCTCCCGCCCCGACATCCCCGACAACATCGTCTTCACCAGCGCGAAGTTCCGGGAGGTCTACGACCTCACG
GTCCAGGTGGCCGGCAGCGACGCCAACATCCTGGTCCTCGGCGAATCGGGTACCGGCAAGGAGCTTATCGCGGGCGCGAT
CCACGTGAACAGCCAGCGCAAGGGGCGGCGCTTTCTCTCCATCAACTGTGCGGCCCTGTCTGACACGCTCCTGGAGAGCC
AGCTCTTCGGCCACGTGCGCGGTGCCTTCACCGGCGCCATCACCACCCAGAAGGGGCTCCTGGAGGAGGCCGACGGCGGC
ACCCTCTTCCTGGACGAGATCGGCGATGTCTCCGCCGCGGTTCAGGCGAAGCTGCTGCGGGTCATCCAGGAGCGGGAGTT
CATCCCCGTGGGGGCCACCAAGCCCAAGAGCGTCGACATCCGCTTCGTGGCCGCCACCAACAAGGATCTGGCCAAGGAGG
TCGCGGCCGGACGATTCCGGGAGGACCTCTACTACCGGCTCAACGTCATCACCATCGCCCTCCCCCCTCTGCGGGAGCGG
CGCGAGGACATCGAGCCCCTGGCCATGCACTTCCTTGCCAAGTATGCCCGCCGCATCCGGAAGAATATCCGCGGGTTCAC
GCCCGAGGCGCTCCAGTCCCTCCAGGGGTACCGCTGGCCCGGCAACGTGCGGGAGCTGGAAAACGTCGTCGAGCGGGCCG
CCATCCTCACCCGGGGCGACCTGGTCACTCCCGAAGTCCTCCCCATCCGCCCCCGGGAGGATGCGCCACTTCTCCGGCCG
GACAACCTGCTCGTTTCCCTGGAGGAGATGGAGCGGGAGCACATCGAGCGGGTCTTGCGCCAGACAGGCTTCCACAAGAG
CCGCACGGCGGAGATCCTCGGCATCTCCCGCAAGACCCTCGACCGCAAGATCGTGGAGTATGCCCTCGCCATCCCCGGCC
AAGGGATCTCCGGAACCCTGGACGACTGA

Upstream 100 bases:

>100_bases
CTTGTCTTGCGGAATCCTGGCCGCGTCTGTATCATTTTGTCCATGTAGACATACTAAAAACTAATGAAACGAACGGCATC
GGCGGGCACGGAAAATCATC

Downstream 100 bases:

>100_bases
ATGGGACCGAAGCGCTTCCCCATCCGCCTCAAGCTCACCTTCGCCACCCTCATCCCCCTCTTCGTGGCCTCGGTCATCTG
CTGGGTCATCGGCATCTCCA

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MKARILICDDEEGIRRYLQKMFLAKEYDVETFADGTSLLARIEGGTEGDADILLQDVRMPDMDGIEVLKRVKKLRPAMPV
VVMTAFGTIDAAVEAIKLGAYDYVTKPFPKEKILGLMENALQLDLLMKENRVLKEELSRPDIPDNIVFTSAKFREVYDLT
VQVAGSDANILVLGESGTGKELIAGAIHVNSQRKGRRFLSINCAALSDTLLESQLFGHVRGAFTGAITTQKGLLEEADGG
TLFLDEIGDVSAAVQAKLLRVIQEREFIPVGATKPKSVDIRFVAATNKDLAKEVAAGRFREDLYYRLNVITIALPPLRER
REDIEPLAMHFLAKYARRIRKNIRGFTPEALQSLQGYRWPGNVRELENVVERAAILTRGDLVTPEVLPIRPREDAPLLRP
DNLLVSLEEMEREHIERVLRQTGFHKSRTAEILGISRKTLDRKIVEYALAIPGQGISGTLDD

Sequences:

>Translated_462_residues
MKARILICDDEEGIRRYLQKMFLAKEYDVETFADGTSLLARIEGGTEGDADILLQDVRMPDMDGIEVLKRVKKLRPAMPV
VVMTAFGTIDAAVEAIKLGAYDYVTKPFPKEKILGLMENALQLDLLMKENRVLKEELSRPDIPDNIVFTSAKFREVYDLT
VQVAGSDANILVLGESGTGKELIAGAIHVNSQRKGRRFLSINCAALSDTLLESQLFGHVRGAFTGAITTQKGLLEEADGG
TLFLDEIGDVSAAVQAKLLRVIQEREFIPVGATKPKSVDIRFVAATNKDLAKEVAAGRFREDLYYRLNVITIALPPLRER
REDIEPLAMHFLAKYARRIRKNIRGFTPEALQSLQGYRWPGNVRELENVVERAAILTRGDLVTPEVLPIRPREDAPLLRP
DNLLVSLEEMEREHIERVLRQTGFHKSRTAEILGISRKTLDRKIVEYALAIPGQGISGTLDD
>Mature_462_residues
MKARILICDDEEGIRRYLQKMFLAKEYDVETFADGTSLLARIEGGTEGDADILLQDVRMPDMDGIEVLKRVKKLRPAMPV
VVMTAFGTIDAAVEAIKLGAYDYVTKPFPKEKILGLMENALQLDLLMKENRVLKEELSRPDIPDNIVFTSAKFREVYDLT
VQVAGSDANILVLGESGTGKELIAGAIHVNSQRKGRRFLSINCAALSDTLLESQLFGHVRGAFTGAITTQKGLLEEADGG
TLFLDEIGDVSAAVQAKLLRVIQEREFIPVGATKPKSVDIRFVAATNKDLAKEVAAGRFREDLYYRLNVITIALPPLRER
REDIEPLAMHFLAKYARRIRKNIRGFTPEALQSLQGYRWPGNVRELENVVERAAILTRGDLVTPEVLPIRPREDAPLLRP
DNLLVSLEEMEREHIERVLRQTGFHKSRTAEILGISRKTLDRKIVEYALAIPGQGISGTLDD

Specific function: Member of the two-component regulatory system zraS/zraR. When activated by zraS it acts in conjunction with sigma-54 to regulate the expression of zraP. Positively autoregulates the expression of the zraSR operon [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=455, Percent_Identity=44.3956043956044, Blast_Score=373, Evalue=1e-104,
Organism=Escherichia coli, GI1790437, Length=440, Percent_Identity=45.4545454545455, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1790299, Length=470, Percent_Identity=39.3617021276596, Blast_Score=322, Evalue=4e-89,
Organism=Escherichia coli, GI1788905, Length=384, Percent_Identity=40.625, Blast_Score=295, Evalue=3e-81,
Organism=Escherichia coli, GI1789233, Length=327, Percent_Identity=44.954128440367, Blast_Score=264, Evalue=1e-71,
Organism=Escherichia coli, GI87082117, Length=342, Percent_Identity=45.3216374269006, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1789087, Length=323, Percent_Identity=48.9164086687306, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI87082152, Length=294, Percent_Identity=45.2380952380952, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI87081872, Length=308, Percent_Identity=42.5324675324675, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1786524, Length=318, Percent_Identity=38.3647798742138, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1787583, Length=310, Percent_Identity=38.0645161290323, Blast_Score=188, Evalue=8e-49,
Organism=Escherichia coli, GI1789828, Length=296, Percent_Identity=36.8243243243243, Blast_Score=164, Evalue=9e-42,
Organism=Escherichia coli, GI87081858, Length=293, Percent_Identity=32.0819112627986, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI1786784, Length=111, Percent_Identity=30.6306306306306, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51600; Mature: 51600

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKARILICDDEEGIRRYLQKMFLAKEYDVETFADGTSLLARIEGGTEGDADILLQDVRMP
CCEEEEEECCHHHHHHHHHHHHHHHHCCCHHHCCCHHHHEEECCCCCCCHHHHHHHCCCC
DMDGIEVLKRVKKLRPAMPVVVMTAFGTIDAAVEAIKLGAYDYVTKPFPKEKILGLMENA
CCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHH
LQLDLLMKENRVLKEELSRPDIPDNIVFTSAKFREVYDLTVQVAGSDANILVLGESGTGK
HHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHHEEEEEEEEEECCCCEEEEEECCCCCH
ELIAGAIHVNSQRKGRRFLSINCAALSDTLLESQLFGHVRGAFTGAITTQKGLLEEADGG
HHHEEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCC
TLFLDEIGDVSAAVQAKLLRVIQEREFIPVGATKPKSVDIRFVAATNKDLAKEVAAGRFR
EEEECCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCEEEEEEEECCHHHHHHHHHHHHH
EDLYYRLNVITIALPPLRERREDIEPLAMHFLAKYARRIRKNIRGFTPEALQSLQGYRWP
HHHHEEEEEEEEECCCHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCC
GNVRELENVVERAAILTRGDLVTPEVLPIRPREDAPLLRPDNLLVSLEEMEREHIERVLR
CCHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCHHEEEHHHHHHHHHHHHHH
QTGFHKSRTAEILGISRKTLDRKIVEYALAIPGQGISGTLDD
HCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKARILICDDEEGIRRYLQKMFLAKEYDVETFADGTSLLARIEGGTEGDADILLQDVRMP
CCEEEEEECCHHHHHHHHHHHHHHHHCCCHHHCCCHHHHEEECCCCCCCHHHHHHHCCCC
DMDGIEVLKRVKKLRPAMPVVVMTAFGTIDAAVEAIKLGAYDYVTKPFPKEKILGLMENA
CCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHH
LQLDLLMKENRVLKEELSRPDIPDNIVFTSAKFREVYDLTVQVAGSDANILVLGESGTGK
HHHHHHHHHHHHHHHHCCCCCCCCCEEEECHHHHEEEEEEEEEECCCCEEEEEECCCCCH
ELIAGAIHVNSQRKGRRFLSINCAALSDTLLESQLFGHVRGAFTGAITTQKGLLEEADGG
HHHEEEEEECCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCC
TLFLDEIGDVSAAVQAKLLRVIQEREFIPVGATKPKSVDIRFVAATNKDLAKEVAAGRFR
EEEECCCCCHHHHHHHHHHHHHHHCCCEECCCCCCCCEEEEEEEECCHHHHHHHHHHHHH
EDLYYRLNVITIALPPLRERREDIEPLAMHFLAKYARRIRKNIRGFTPEALQSLQGYRWP
HHHHEEEEEEEEECCCHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCC
GNVRELENVVERAAILTRGDLVTPEVLPIRPREDAPLLRPDNLLVSLEEMEREHIERVLR
CCHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCCCCCCHHEEEHHHHHHHHHHHHHH
QTGFHKSRTAEILGISRKTLDRKIVEYALAIPGQGISGTLDD
HCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11243806 [H]