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The map label for this gene is zraS [H]

Identifier: 78222275

GI number: 78222275

Start: 1173596

End: 1175617

Strand: Reverse

Name: zraS [H]

Synonym: Gmet_1057

Alternate gene names: 78222275

Gene position: 1175617-1173596 (Counterclockwise)

Preceding gene: 78222276

Following gene: 78222274

Centisome position: 29.41

GC content: 65.18

Gene sequence:

>2022_bases
ATGGGACCGAAGCGCTTCCCCATCCGCCTCAAGCTCACCTTCGCCACCCTCATCCCCCTCTTCGTGGCCTCGGTCATCTG
CTGGGTCATCGGCATCTCCATCATCAACTCCCGGATCGTGACCCAGGCCCAGGAAAAGGTCCGCACCGACCTGAACTCGG
CCCGGGCCGTCTACCAGAGCGAACTTGACCACATCCGGGACGTGGTCAAGTTCACCGCCACCAACCCCTTCACCTCCATG
ATTATCTCCCAGGGGGACCGCGCGACCCTCAACAACCTCCTCTCACCGCGGATGGCCAGCGAAGGGCTCGACTTCCTCAC
CGCCGTGGACCGGTCGGGCCGGGTCGTCTTCCGGGCCAGCAACCACGGGGCCTTCGGCGACAGCAAGATCAACGACCAGA
TCGTCTCCCGGGCGTTGCAGGGCGAGCACATCGCCGGCACCGAGGTGATCCCCCCCGGGGAACTGGTGAAAGAAGGGAAA
GAGCTGGCCGCCAAGGCGTCCATCCAGATCCTCCCCACCCTCCACGCCCGCCAGCGGGACGAGTCCGTGGAGCGCTCCGG
CATGGCCATGGTGGTATCGGTGCCGGTGCGGGACCAGGCCGGGACGGTCGCGGGTGCCCTCTACGGAGGAGTTCTCCTCA
ACGGCAACAACACCCTGGTGGACCGGATCAAGAAGATCGTCTTCGAAGCGGTGAGCTACCAGGGGGAGGACGTGGGAACC
GCCACCATCTTCCTGGGGGACTTGCGGATAGCCACCAACGTCCTCCTTTCGCCGGAACGGCGGGCCATCGGCACGAGGCT
CTCGGAGGAGGTCTACAACCGGGTCCTCCTGGCCCGCGAGAAATGGGTGGGACGGGCCTTTGTGGTGAACGACTGGTACT
ACTCCGCCTACGAGCCGATCCTGAACCTCCAGGGGAAGCCGGTGGGATCCCTCTACGTGGGGGTCATGGAACGCCCCTTC
CTGGAGATGAAACGGGAGATATCCCTCATCTTCGGGGTGGTCCTCCTCTGCGGCTCCCTCATCGGGCTGGCCATTTCGAG
CTTCATCGCCTCGCGCCTCGCCCGCCCCATCAAGGAACTGGAAAACGCAGCCCGCCGGATCACCGCCGGCGAACGCGACA
GTGCCATAACCATCCAGACCCGCGACGAGATCGGCGACCTGGCAGGGGAGTTCGCCCAGATGACCAGAACCCTCGCCCAG
CGGGAGGAGGACATCCGGAAGCTGAACCGGGACCTGGAGCAGAAGGTGCTGGACCGCACGGCCCAGCTGGAGGAAAAGAA
CCTCCTTCTTGTGAAGACCCAGGAGGACCTGGTCAGGGCGGCAAAGCTGGCCGACATCGGCATGCTGGCCGCCGGCGTGG
CCCACGAGATCAACAACCCCCTGGCGATCATCCGGGGGAACACGGAGCTGCTCCAGATGGCGATCCCCGAGGACGACCCG
AGCCGGGAGGAGGTGGACACCATCGCCCAGCAGACGGGACGGGTGGAGCGGATCGTGGGGAGCCTCCTCAAGTTCGCCCG
CCAGCAGAAGAAGGAACTGGGAACGGTGGAGGTCAACAGGCTCCTGGATGAGATCCTCAAGCAGGTGGGGCACCAGATTC
CCCTTGCCGGCATCGACATCCGACGGGACTACGCCGCCGACATCCCCCTTCTGGAAGGAGACGGCGACCAGCTCCGCCAG
GTCTTCACCAACCTCATCCTCAACGCCATCCAGGCCATGAAGGAGGGGGGAACTCTCACGGTGGGCACCTCCCGGGACAG
CGAAGCCGGGACCTGTACCGTTGCCGTGGAGGACACGGGGGCCGGCATCCCCCCCGAAAACCTCAGGAACATCTTCAGCC
CCTTCTTCACCACCCGCAACGACGGGACCGGCCTCGGCCTCGCGGTTTCCTACGGCATCGTCAGGGATCACGGGGGGAAT
ATCCAGGTGGAGAGCACGGCGGGGACGGGGAGCACCTTCCGGGTGGTGCTGCCGCTGAAGCAGGGGGGGACGACTGACGT
AAGCATCAGACAAGGGACTTAA

Upstream 100 bases:

>100_bases
GGAGATCCTCGGCATCTCCCGCAAGACCCTCGACCGCAAGATCGTGGAGTATGCCCTCGCCATCCCCGGCCAAGGGATCT
CCGGAACCCTGGACGACTGA

Downstream 100 bases:

>100_bases
CTGCCTGATAGGGCTTGAAGCAGAACATTAAGGACAATACATCATGGACTTCAAAAGCGTACTCGACAAACTCCTCTCAT
CCTTCGACGATCAGGGCATC

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 673; Mature: 672

Protein sequence:

>673_residues
MGPKRFPIRLKLTFATLIPLFVASVICWVIGISIINSRIVTQAQEKVRTDLNSARAVYQSELDHIRDVVKFTATNPFTSM
IISQGDRATLNNLLSPRMASEGLDFLTAVDRSGRVVFRASNHGAFGDSKINDQIVSRALQGEHIAGTEVIPPGELVKEGK
ELAAKASIQILPTLHARQRDESVERSGMAMVVSVPVRDQAGTVAGALYGGVLLNGNNTLVDRIKKIVFEAVSYQGEDVGT
ATIFLGDLRIATNVLLSPERRAIGTRLSEEVYNRVLLAREKWVGRAFVVNDWYYSAYEPILNLQGKPVGSLYVGVMERPF
LEMKREISLIFGVVLLCGSLIGLAISSFIASRLARPIKELENAARRITAGERDSAITIQTRDEIGDLAGEFAQMTRTLAQ
REEDIRKLNRDLEQKVLDRTAQLEEKNLLLVKTQEDLVRAAKLADIGMLAAGVAHEINNPLAIIRGNTELLQMAIPEDDP
SREEVDTIAQQTGRVERIVGSLLKFARQQKKELGTVEVNRLLDEILKQVGHQIPLAGIDIRRDYAADIPLLEGDGDQLRQ
VFTNLILNAIQAMKEGGTLTVGTSRDSEAGTCTVAVEDTGAGIPPENLRNIFSPFFTTRNDGTGLGLAVSYGIVRDHGGN
IQVESTAGTGSTFRVVLPLKQGGTTDVSIRQGT

Sequences:

>Translated_673_residues
MGPKRFPIRLKLTFATLIPLFVASVICWVIGISIINSRIVTQAQEKVRTDLNSARAVYQSELDHIRDVVKFTATNPFTSM
IISQGDRATLNNLLSPRMASEGLDFLTAVDRSGRVVFRASNHGAFGDSKINDQIVSRALQGEHIAGTEVIPPGELVKEGK
ELAAKASIQILPTLHARQRDESVERSGMAMVVSVPVRDQAGTVAGALYGGVLLNGNNTLVDRIKKIVFEAVSYQGEDVGT
ATIFLGDLRIATNVLLSPERRAIGTRLSEEVYNRVLLAREKWVGRAFVVNDWYYSAYEPILNLQGKPVGSLYVGVMERPF
LEMKREISLIFGVVLLCGSLIGLAISSFIASRLARPIKELENAARRITAGERDSAITIQTRDEIGDLAGEFAQMTRTLAQ
REEDIRKLNRDLEQKVLDRTAQLEEKNLLLVKTQEDLVRAAKLADIGMLAAGVAHEINNPLAIIRGNTELLQMAIPEDDP
SREEVDTIAQQTGRVERIVGSLLKFARQQKKELGTVEVNRLLDEILKQVGHQIPLAGIDIRRDYAADIPLLEGDGDQLRQ
VFTNLILNAIQAMKEGGTLTVGTSRDSEAGTCTVAVEDTGAGIPPENLRNIFSPFFTTRNDGTGLGLAVSYGIVRDHGGN
IQVESTAGTGSTFRVVLPLKQGGTTDVSIRQGT
>Mature_672_residues
GPKRFPIRLKLTFATLIPLFVASVICWVIGISIINSRIVTQAQEKVRTDLNSARAVYQSELDHIRDVVKFTATNPFTSMI
ISQGDRATLNNLLSPRMASEGLDFLTAVDRSGRVVFRASNHGAFGDSKINDQIVSRALQGEHIAGTEVIPPGELVKEGKE
LAAKASIQILPTLHARQRDESVERSGMAMVVSVPVRDQAGTVAGALYGGVLLNGNNTLVDRIKKIVFEAVSYQGEDVGTA
TIFLGDLRIATNVLLSPERRAIGTRLSEEVYNRVLLAREKWVGRAFVVNDWYYSAYEPILNLQGKPVGSLYVGVMERPFL
EMKREISLIFGVVLLCGSLIGLAISSFIASRLARPIKELENAARRITAGERDSAITIQTRDEIGDLAGEFAQMTRTLAQR
EEDIRKLNRDLEQKVLDRTAQLEEKNLLLVKTQEDLVRAAKLADIGMLAAGVAHEINNPLAIIRGNTELLQMAIPEDDPS
REEVDTIAQQTGRVERIVGSLLKFARQQKKELGTVEVNRLLDEILKQVGHQIPLAGIDIRRDYAADIPLLEGDGDQLRQV
FTNLILNAIQAMKEGGTLTVGTSRDSEAGTCTVAVEDTGAGIPPENLRNIFSPFFTTRNDGTGLGLAVSYGIVRDHGGNI
QVESTAGTGSTFRVVLPLKQGGTTDVSIRQGT

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=226, Percent_Identity=39.8230088495575, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1788549, Length=241, Percent_Identity=34.4398340248963, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1790300, Length=239, Percent_Identity=33.0543933054393, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI87081816, Length=353, Percent_Identity=29.1784702549575, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1789149, Length=353, Percent_Identity=27.1954674220963, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1788393, Length=319, Percent_Identity=25.3918495297806, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI145693157, Length=249, Percent_Identity=30.1204819277108, Blast_Score=89, Evalue=1e-18,
Organism=Escherichia coli, GI1786600, Length=293, Percent_Identity=27.3037542662116, Blast_Score=86, Evalue=9e-18,
Organism=Escherichia coli, GI1786912, Length=216, Percent_Identity=28.2407407407407, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1790346, Length=220, Percent_Identity=26.3636363636364, Blast_Score=80, Evalue=6e-16,
Organism=Escherichia coli, GI87082128, Length=223, Percent_Identity=26.9058295964126, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI48994928, Length=111, Percent_Identity=38.7387387387387, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787894, Length=239, Percent_Identity=28.8702928870293, Blast_Score=73, Evalue=7e-14,
Organism=Escherichia coli, GI1790861, Length=206, Percent_Identity=31.0679611650485, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1786783, Length=223, Percent_Identity=27.8026905829596, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1789808, Length=238, Percent_Identity=26.0504201680672, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 73492; Mature: 73361

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPKRFPIRLKLTFATLIPLFVASVICWVIGISIINSRIVTQAQEKVRTDLNSARAVYQS
CCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELDHIRDVVKFTATNPFTSMIISQGDRATLNNLLSPRMASEGLDFLTAVDRSGRVVFRAS
HHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHCCCHHHHCCCHHHHHHCCCCCEEEEEC
NHGAFGDSKINDQIVSRALQGEHIAGTEVIPPGELVKEGKELAAKASIQILPTLHARQRD
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECHHHHHHH
ESVERSGMAMVVSVPVRDQAGTVAGALYGGVLLNGNNTLVDRIKKIVFEAVSYQGEDVGT
HHHHHCCCEEEEEECCCCCCCHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCE
ATIFLGDLRIATNVLLSPERRAIGTRLSEEVYNRVLLAREKWVGRAFVVNDWYYSAYEPI
EEEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHH
LNLQGKPVGSLYVGVMERPFLEMKREISLIFGVVLLCGSLIGLAISSFIASRLARPIKEL
HCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENAARRITAGERDSAITIQTRDEIGDLAGEFAQMTRTLAQREEDIRKLNRDLEQKVLDRT
HHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQLEEKNLLLVKTQEDLVRAAKLADIGMLAAGVAHEINNPLAIIRGNTELLQMAIPEDDP
HHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHEEEEECCCCCC
SREEVDTIAQQTGRVERIVGSLLKFARQQKKELGTVEVNRLLDEILKQVGHQIPLAGIDI
CHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEE
RRDYAADIPLLEGDGDQLRQVFTNLILNAIQAMKEGGTLTVGTSRDSEAGTCTVAVEDTG
CCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEECCC
AGIPPENLRNIFSPFFTTRNDGTGLGLAVSYGIVRDHGGNIQVESTAGTGSTFRVVLPLK
CCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECEEEECCCCEEEEECCCCCCEEEEEEEEC
QGGTTDVSIRQGT
CCCCCEEEEECCC
>Mature Secondary Structure 
GPKRFPIRLKLTFATLIPLFVASVICWVIGISIINSRIVTQAQEKVRTDLNSARAVYQS
CCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELDHIRDVVKFTATNPFTSMIISQGDRATLNNLLSPRMASEGLDFLTAVDRSGRVVFRAS
HHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHCCCHHHHCCCHHHHHHCCCCCEEEEEC
NHGAFGDSKINDQIVSRALQGEHIAGTEVIPPGELVKEGKELAAKASIQILPTLHARQRD
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECHHHHHHH
ESVERSGMAMVVSVPVRDQAGTVAGALYGGVLLNGNNTLVDRIKKIVFEAVSYQGEDVGT
HHHHHCCCEEEEEECCCCCCCHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHCCCCCCCE
ATIFLGDLRIATNVLLSPERRAIGTRLSEEVYNRVLLAREKWVGRAFVVNDWYYSAYEPI
EEEEEECHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHH
LNLQGKPVGSLYVGVMERPFLEMKREISLIFGVVLLCGSLIGLAISSFIASRLARPIKEL
HCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ENAARRITAGERDSAITIQTRDEIGDLAGEFAQMTRTLAQREEDIRKLNRDLEQKVLDRT
HHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQLEEKNLLLVKTQEDLVRAAKLADIGMLAAGVAHEINNPLAIIRGNTELLQMAIPEDDP
HHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHEEEEECCCCCC
SREEVDTIAQQTGRVERIVGSLLKFARQQKKELGTVEVNRLLDEILKQVGHQIPLAGIDI
CHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCEEE
RRDYAADIPLLEGDGDQLRQVFTNLILNAIQAMKEGGTLTVGTSRDSEAGTCTVAVEDTG
CCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEECCC
AGIPPENLRNIFSPFFTTRNDGTGLGLAVSYGIVRDHGGNIQVESTAGTGSTFRVVLPLK
CCCCHHHHHHHHHHHHCCCCCCCCCCCEEEECEEEECCCCEEEEECCCCCCEEEEEEEEC
QGGTTDVSIRQGT
CCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]