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The map label for this gene is narI [H]

Identifier: 78222242

GI number: 78222242

Start: 1139699

End: 1140481

Strand: Direct

Name: narI [H]

Synonym: Gmet_1022

Alternate gene names: 78222242

Gene position: 1139699-1140481 (Clockwise)

Preceding gene: 78222241

Following gene: 78222243

Centisome position: 28.51

GC content: 58.75

Gene sequence:

>783_bases
ATGCCGGTCGATTCCGGACTGTCGGACCATATCTGCGCCATCCTCCGGTTTCTTGCCCGTCCGGCCGGAACAGCCCCGAA
CAGCGGTGACCCTATGACTGACTATCTCTTTTTCGGTGTTTTCCCCTACGTTGCCCTCGTTGTCGCAGTGGGCGGCGGGG
TGTATCGGTATGCGAAGGAACGGTTTTCCTTTTCAAGCCTCTCTTCCCAGTTTCTGGAGAACCGGGCCCTTTTCTGGGGG
AGTGTTGCCTGGCATTACGGCATTGTCCTGGTTCTCCTCGCCCATCTCGCGGCACTGCTCCTCCCCGCATCCTGGGGGCG
CCTCCTGGGCGACCCGCTCCGTCTCGCCGTCCTTGAAGTGACCGGCCTTGCCCTCGGGCTGTTCACCCTTGCCGGCATCG
TGGTCCTTCTGTTCAGGCGGATCGCCAGTCCGAAGGCGTGGGCGGTTGTCACGGTCATGGACTTGTTCCTGTTGGCCGTC
CTTGTTCTTCAAGTGGGTACCGGACTTTACAATGCTTTCGTGTATCGCTGGGGTTCCCTCTGGTATCTTTCCCTGGCCGT
CCCCTGGCTCCACTCCCTGGCATGCCTGAACCCGGATATCAAATACATTGTTCCACTCCCGTTGCCGGTTAAAGTGCACC
TGTTCAACGCCTTTGTCATCTTTGTTCTCTTTCCGGTCACCCGGCTGGTGCACATCGTGTCGATACCCGTTTCCTATCTC
TGGCGCCCCTGGCAGATAGCTGTCTGGAATAAGCCTCCGCGTTGGATGATCCCAAGGAAGTGA

Upstream 100 bases:

>100_bases
GAGATGGCGATGGAAATGTCGGTCGATCCGGCTGACCGGAAAGGGGAGGCGGGAACGGGATTCCTTCGGCCTCCGAAAAG
GGGATGGTGATGGCCACGAT

Downstream 100 bases:

>100_bases
ACGGGATCGTGGTGGAGAGCGAGCAGGGCAGGGGAACGACCGTGCGGGTCCTGATTCCGCGGACCGAGCAGATCGAACGG
GCACTCGCATCGGCCCGCAC

Product: respiratory nitrate reductase subunit gamma

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MPVDSGLSDHICAILRFLARPAGTAPNSGDPMTDYLFFGVFPYVALVVAVGGGVYRYAKERFSFSSLSSQFLENRALFWG
SVAWHYGIVLVLLAHLAALLLPASWGRLLGDPLRLAVLEVTGLALGLFTLAGIVVLLFRRIASPKAWAVVTVMDLFLLAV
LVLQVGTGLYNAFVYRWGSLWYLSLAVPWLHSLACLNPDIKYIVPLPLPVKVHLFNAFVIFVLFPVTRLVHIVSIPVSYL
WRPWQIAVWNKPPRWMIPRK

Sequences:

>Translated_260_residues
MPVDSGLSDHICAILRFLARPAGTAPNSGDPMTDYLFFGVFPYVALVVAVGGGVYRYAKERFSFSSLSSQFLENRALFWG
SVAWHYGIVLVLLAHLAALLLPASWGRLLGDPLRLAVLEVTGLALGLFTLAGIVVLLFRRIASPKAWAVVTVMDLFLLAV
LVLQVGTGLYNAFVYRWGSLWYLSLAVPWLHSLACLNPDIKYIVPLPLPVKVHLFNAFVIFVLFPVTRLVHIVSIPVSYL
WRPWQIAVWNKPPRWMIPRK
>Mature_259_residues
PVDSGLSDHICAILRFLARPAGTAPNSGDPMTDYLFFGVFPYVALVVAVGGGVYRYAKERFSFSSLSSQFLENRALFWGS
VAWHYGIVLVLLAHLAALLLPASWGRLLGDPLRLAVLEVTGLALGLFTLAGIVVLLFRRIASPKAWAVVTVMDLFLLAVL
VLQVGTGLYNAFVYRWGSLWYLSLAVPWLHSLACLNPDIKYIVPLPLPVKVHLFNAFVIFVLFPVTRLVHIVSIPVSYLW
RPWQIAVWNKPPRWMIPRK

Specific function: The Nitrate Reductase Enzyme Complex Allows E.Coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Gamma Chain Is A Membrane-Embedded Heme-Iron Unit Resembling Cytochrome B, Which Transfers Electrons From Quinones To The Beta Subunit.

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787480, Length=219, Percent_Identity=29.2237442922374, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787738, Length=221, Percent_Identity=28.5067873303167, Blast_Score=78, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 29027; Mature: 28896

Theoretical pI: Translated: 10.23; Mature: 10.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
PVDSGLSDHICAILRFLARPAGTAPNSGDPMTDYLFFGVFPYVALVVAVGGGVYRYAKE
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
RFSFSSLSSQFLENRALFWGSVAWHYGIVLVLLAHLAALLLPASWGRLLGDPLRLAVLEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
TGLALGLFTLAGIVVLLFRRIASPKAWAVVTVMDLFLLAVLVLQVGTGLYNAFVYRWGSL
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WYLSLAVPWLHSLACLNPDIKYIVPLPLPVKVHLFNAFVIFVLFPVTRLVHIVSIPVSYL
HHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
WRPWQIAVWNKPPRWMIPRK
CCCEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 9384377; 7557333 [H]