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The map label for this gene is narH [H]

Identifier: 78222241

GI number: 78222241

Start: 1138201

End: 1139688

Strand: Direct

Name: narH [H]

Synonym: Gmet_1021

Alternate gene names: 78222241

Gene position: 1138201-1139688 (Clockwise)

Preceding gene: 78222240

Following gene: 78222242

Centisome position: 28.47

GC content: 63.44

Gene sequence:

>1488_bases
ATGAAAGTCCGTGCCCAGGTTTCCATGGTGTTCAACCTGGACAAGTGTATCGGCTGCCACACGTGCAGCATCGCCTGCAA
GAACATCTGGAGCGACCGCCGGGGCGCCGAGTACATGTGGTGGAACAACGTGGAGACGAAGCCGGGGACCGGCTATCCGA
CCCTCTGGGAGGACCAGGAGAAGTACCGGGGAGGGTGGGAGCGAACCGACAGGGAACCGCGCCTCCGGCTCCACTCCCGG
GGTGGCGGATTGGCGAAGCTATTCTTCAATCCCCGTCTCCCGACCATTGACGACTATTACGAGCCGTGGAGCTACCGGTA
CGGCGATCTCCTCAGCGCCCCGGCCGGGGATGACCAGCCGGTGGCACGCCCGGTCTCCCTGGTGACCGGAGACCCCATCA
ACATCGAGGCCGGGCCCAACTGGGACGACGATCTCGGGGGCTCTCCCGTCTATGGGGCTCATGACCCGAACCTTGCTGCC
GTGAGCGAGGCGGAACGGCGTCAACTCTTCCTCCTGGAGCGGATGGTCCTCTTCTATCTTCCCCGGATCTGCAACCACTG
CGCGAATCCCGGGTGCGTGGCCGCCTGTCCTTCCGGGGCCATCTACAAGCGGGGCGAAGACGGCATTGTCCTGGTGAGTC
AGGAGAAGTGCCGGGGCTGGCGCATGTGCGTTTCGGGATGTCCCTACAAGAAGGTCTATTACAATTGGGGTAGCGGTAAG
TCGGAAAAATGCATCCTCTGCTATCCCCGCCAGGAGACCGGCCAGGCCCCGGCCTGTTTCCATTCCTGCGTCGGGCGGCT
CCGCTACCTGGGAGTGCTCCTCTACGATGCGGAGCGAATCGAACGGAGTGCTTCGCGCCCCGACCACGACCTGGTGCAGG
CGCAACGGGAGATGATTCTCGACCCCTCCGATCCCGAGGTTGTCAATAAGGCGGCGGAAGAGGGGATTCACCCAGCGGTC
CTGGCCGCTGCCCGTAATTCGCCGGTCTATCGATTCGTCAAGGAGTGGCGGCTGGCACTGCCGCTTCATCCCGAATTCGG
CACCGTCCCGATGCTCTATTACGTCCCTCCGCTCCTGCCGCTTGCCGCCGATCGGGAAAATGGCTGCTATGACCTCGGGG
GGGAGTTCTTCACCTCCCTAGAGCAGGGGCGCTTGCCGCTTCGCTATCTCGCATCCCTCTTCTCGGCAGGCAATGAAGAA
ATCATCCGCGCGGTCTACCGGAAACTCACGGCGGTCCGTGTTCACCGTCGAGCGGCCACCACGGGAGATGTGCCGGCCGG
CGAGTTGGAACGGTTCCTCGCGGAAGCGGGCACCACCCCGGAGGAAGCCGAGGCGATCTACCATATGACGTCACTTGCCG
GGTTTGATGAGCGGCACGCCGTTCCCCCCTTCATGCGGGAGATGGCGATGGAAATGTCGGTCGATCCGGCTGACCGGAAA
GGGGAGGCGGGAACGGGATTCCTTCGGCCTCCGAAAAGGGGATGGTGA

Upstream 100 bases:

>100_bases
GGGACACCTACGTGTATGTGCACAAGCTTGAGGGGGCCCTTCGCTATTGAGGGGAGAGGGAACGGTTATTCCCGTCGGGA
TCTGAGGAAGGAGAAGCGCC

Downstream 100 bases:

>100_bases
TGGCCACGATATGCCGGTCGATTCCGGACTGTCGGACCATATCTGCGCCATCCTCCGGTTTCTTGCCCGTCCGGCCGGAA
CAGCCCCGAACAGCGGTGAC

Product: respiratory nitrate reductase beta subunit

Products: NA

Alternate protein names: Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MKVRAQVSMVFNLDKCIGCHTCSIACKNIWSDRRGAEYMWWNNVETKPGTGYPTLWEDQEKYRGGWERTDREPRLRLHSR
GGGLAKLFFNPRLPTIDDYYEPWSYRYGDLLSAPAGDDQPVARPVSLVTGDPINIEAGPNWDDDLGGSPVYGAHDPNLAA
VSEAERRQLFLLERMVLFYLPRICNHCANPGCVAACPSGAIYKRGEDGIVLVSQEKCRGWRMCVSGCPYKKVYYNWGSGK
SEKCILCYPRQETGQAPACFHSCVGRLRYLGVLLYDAERIERSASRPDHDLVQAQREMILDPSDPEVVNKAAEEGIHPAV
LAAARNSPVYRFVKEWRLALPLHPEFGTVPMLYYVPPLLPLAADRENGCYDLGGEFFTSLEQGRLPLRYLASLFSAGNEE
IIRAVYRKLTAVRVHRRAATTGDVPAGELERFLAEAGTTPEEAEAIYHMTSLAGFDERHAVPPFMREMAMEMSVDPADRK
GEAGTGFLRPPKRGW

Sequences:

>Translated_495_residues
MKVRAQVSMVFNLDKCIGCHTCSIACKNIWSDRRGAEYMWWNNVETKPGTGYPTLWEDQEKYRGGWERTDREPRLRLHSR
GGGLAKLFFNPRLPTIDDYYEPWSYRYGDLLSAPAGDDQPVARPVSLVTGDPINIEAGPNWDDDLGGSPVYGAHDPNLAA
VSEAERRQLFLLERMVLFYLPRICNHCANPGCVAACPSGAIYKRGEDGIVLVSQEKCRGWRMCVSGCPYKKVYYNWGSGK
SEKCILCYPRQETGQAPACFHSCVGRLRYLGVLLYDAERIERSASRPDHDLVQAQREMILDPSDPEVVNKAAEEGIHPAV
LAAARNSPVYRFVKEWRLALPLHPEFGTVPMLYYVPPLLPLAADRENGCYDLGGEFFTSLEQGRLPLRYLASLFSAGNEE
IIRAVYRKLTAVRVHRRAATTGDVPAGELERFLAEAGTTPEEAEAIYHMTSLAGFDERHAVPPFMREMAMEMSVDPADRK
GEAGTGFLRPPKRGW
>Mature_495_residues
MKVRAQVSMVFNLDKCIGCHTCSIACKNIWSDRRGAEYMWWNNVETKPGTGYPTLWEDQEKYRGGWERTDREPRLRLHSR
GGGLAKLFFNPRLPTIDDYYEPWSYRYGDLLSAPAGDDQPVARPVSLVTGDPINIEAGPNWDDDLGGSPVYGAHDPNLAA
VSEAERRQLFLLERMVLFYLPRICNHCANPGCVAACPSGAIYKRGEDGIVLVSQEKCRGWRMCVSGCPYKKVYYNWGSGK
SEKCILCYPRQETGQAPACFHSCVGRLRYLGVLLYDAERIERSASRPDHDLVQAQREMILDPSDPEVVNKAAEEGIHPAV
LAAARNSPVYRFVKEWRLALPLHPEFGTVPMLYYVPPLLPLAADRENGCYDLGGEFFTSLEQGRLPLRYLASLFSAGNEE
IIRAVYRKLTAVRVHRRAATTGDVPAGELERFLAEAGTTPEEAEAIYHMTSLAGFDERHAVPPFMREMAMEMSVDPADRK
GEAGTGFLRPPKRGW

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons ar

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=477, Percent_Identity=51.9916142557652, Blast_Score=509, Evalue=1e-145,
Organism=Escherichia coli, GI1787740, Length=472, Percent_Identity=49.7881355932203, Blast_Score=483, Evalue=1e-137,
Organism=Escherichia coli, GI1787122, Length=91, Percent_Identity=43.956043956044, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787872, Length=99, Percent_Identity=42.4242424242424, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1787749, Length=80, Percent_Identity=40, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1790326, Length=163, Percent_Identity=32.5153374233129, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789370, Length=91, Percent_Identity=37.3626373626374, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 55575; Mature: 55575

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVRAQVSMVFNLDKCIGCHTCSIACKNIWSDRRGAEYMWWNNVETKPGTGYPTLWEDQE
CCCCEEEHEEEEHHHHHCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCHH
KYRGGWERTDREPRLRLHSRGGGLAKLFFNPRLPTIDDYYEPWSYRYGDLLSAPAGDDQP
HHCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCCHHCCCCCCCCCCCHHCCCCCCCCC
VARPVSLVTGDPINIEAGPNWDDDLGGSPVYGAHDPNLAAVSEAERRQLFLLERMVLFYL
CCCCEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
PRICNHCANPGCVAACPSGAIYKRGEDGIVLVSQEKCRGWRMCVSGCPYKKVYYNWGSGK
HHHHHHCCCCCCEEECCCCCEEECCCCCEEEEEHHHCCHHHHHHCCCCCCEEEEECCCCC
SEKCILCYPRQETGQAPACFHSCVGRLRYLGVLLYDAERIERSASRPDHDLVQAQREMIL
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
DPSDPEVVNKAAEEGIHPAVLAAARNSPVYRFVKEWRLALPLHPEFGTVPMLYYVPPLLP
CCCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCC
LAADRENGCYDLGGEFFTSLEQGRLPLRYLASLFSAGNEEIIRAVYRKLTAVRVHRRAAT
CCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
TGDVPAGELERFLAEAGTTPEEAEAIYHMTSLAGFDERHAVPPFMREMAMEMSVDPADRK
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCC
GEAGTGFLRPPKRGW
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MKVRAQVSMVFNLDKCIGCHTCSIACKNIWSDRRGAEYMWWNNVETKPGTGYPTLWEDQE
CCCCEEEHEEEEHHHHHCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCHH
KYRGGWERTDREPRLRLHSRGGGLAKLFFNPRLPTIDDYYEPWSYRYGDLLSAPAGDDQP
HHCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCCHHCCCCCCCCCCCHHCCCCCCCCC
VARPVSLVTGDPINIEAGPNWDDDLGGSPVYGAHDPNLAAVSEAERRQLFLLERMVLFYL
CCCCEEEECCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
PRICNHCANPGCVAACPSGAIYKRGEDGIVLVSQEKCRGWRMCVSGCPYKKVYYNWGSGK
HHHHHHCCCCCCEEECCCCCEEECCCCCEEEEEHHHCCHHHHHHCCCCCCEEEEECCCCC
SEKCILCYPRQETGQAPACFHSCVGRLRYLGVLLYDAERIERSASRPDHDLVQAQREMIL
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
DPSDPEVVNKAAEEGIHPAVLAAARNSPVYRFVKEWRLALPLHPEFGTVPMLYYVPPLLP
CCCCHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCC
LAADRENGCYDLGGEFFTSLEQGRLPLRYLASLFSAGNEEIIRAVYRKLTAVRVHRRAAT
CCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
TGDVPAGELERFLAEAGTTPEEAEAIYHMTSLAGFDERHAVPPFMREMAMEMSVDPADRK
CCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCC
GEAGTGFLRPPKRGW
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2674654; 8905232; 9278503; 2832376; 3053688; 1321049; 8383531; 8388253; 8664273; 9516445; 12910261 [H]