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The map label for this gene is arnT [H]

Identifier: 78222107

GI number: 78222107

Start: 976641

End: 978305

Strand: Reverse

Name: arnT [H]

Synonym: Gmet_0887

Alternate gene names: 78222107

Gene position: 978305-976641 (Counterclockwise)

Preceding gene: 78222108

Following gene: 78222106

Centisome position: 24.47

GC content: 62.4

Gene sequence:

>1665_bases
ATGAAAACTGCCTTCACCGTCTCATCCAACCGGGAGCAGAGCCTGTTCAAGGATCTCATCATCCTTGCCGTGGTCTTCGG
CTTTGCCTTCTTCCAACGGCTCGGCCAGATTCCACTTCTCGATTCCGACGAGGGGCGCTACGCCGAAATCCCGCGGGAAA
TGCTCGAACGGGGGGACTTCATAACCCCCTTCCTGAACTACGTGAAGTACTTCGAGAAACCACCGCTCCACTACTGGCTG
GAGGCCATTTCGTTCAGGATATTCGGCTTCAGCGAGTTCGCGGCCCGGGCTCCGGGGGCGCTCATGGGGCTCTGCGGCGT
CCTCTTCACCTATCACGTGGGACGGACTGTTTTTGGTCGACGCGCAGGACTCCTGGCGGCGACGGTTCTCGGCACCTCCC
TCGGCTTTATCGTCATTGGCCGCTACAACGTCATCGACATGACCCTCACCTTCTGGATGACCGCCACCCTCGGCTGCTTT
CTCCTGGCGGTTCACGACAGGGGCCGGCGCGCCGGCCTCTACTACTATCTCTTCTACGTGGCGGCAGCCCTCACCGTGCT
CGCCAAGGGGCTCATCGGCCTCGTCCTCCCCGGCGGCGTGATCGTCCTCTTCATCCTCCTCACGGGGCGCTGGCGGCTTC
TTCGCGAGATGCGGCTCCCCACGGGCACTGTCCTCTTCCTTCTCGTCGCGGCACCCTGGTTCATTCTCGTCTCGCTCCAC
AACCCGGAATTTCCACGGTTTTTCTTCATCCATGAACACTTCGAACGCTTTCTCACCAAGGTTCACGGCCGCTACGAACC
CCCCTGGTTCTTCATTCCGGTCCTGGCCGGGTTCATGCTTCCCTGGTCCTTCTACCTTCCGTCCGCCATTCGGGGCATGT
GGCAGGAGCGACACACTCCCACGGGCGAGGCGCGTCTCTACCTCATCCTCTGGGGAGCGGTCATCTTCACCTTTTTCTCC
CTGTCGAGCTCCAAACTGATCCCGTACATCCTCCCGGTATTCCCGGCGGTGGCGCTTCTCGTTGGCGAGTTCTTCTCACG
CGTGGCCGACCGCCGCGCCACCTTTCCCACCCTGCCGACGGCCATCGCAGCGGCGGTGCTGACGATCGGCGGAGCCGGCG
GCATCCTTTTCCCCCACCTGGCCAAGAACCCGAAACTGTCGGTGCTCGCCGGGGCCGTGATCGGCTCCTGCCTCCTTGCC
CAGGGGATAGCCGCCTTCGTGGGCCTGCGCCGCAAGAAGGCCGCCGTGCTCCTAGTCTCCTTCGGCCTTTTCTCGTACCT
TCTGGGCATCGCCGGCCCGCCGTTCATCCTCGCCCGCACCGCCGAACGGAAGTCATTCCGGGAACTGGGGCAACTGGTGA
AGGAAAAGGCGGGACCGGATACCGCCGTTGCCAGCTTCCAGCTCTACCAGCAAGGATTTTCCTACTATGCGGGACGGCGG
ATCATCATTGTGGGAGAGCGTGGAGAGCTCGATTTCGGGAGCCGCCAGGGGGACCAGAGCCAGTGGTTCTTGGACGAGCA
GGGCTTTGCGCGCCTCTGGGACTCGGGCCGACCGGTCATCGTTCTCATCAAGGAGGATGCCCTTCCCGCCTTGAGAAAAC
TCGTCACGACCCCGCCGCGGGAGTTGGGGAGAAAAGGAAAGGTTCTTCTCATCGCGAACCACTGA

Upstream 100 bases:

>100_bases
CGAACCAGCGGATCCGCGTGAGTCTTCCCGTAGCGGCGGAACGCTACGACCTTATCCGGCGGGAAAAGCCATAGCCCCCT
CATCCAAGAAAGACTACTCC

Downstream 100 bases:

>100_bases
CCCGACGGGGACAGCGTAAACGGTATGGCACTTCGCGCGGTTTTGTGCCATCATGTAGCGTTTTTCCGGAAGGAGGCAAC
CGTGCGCAGCGAATTTCTCC

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: 4-amino-4-deoxy-L-arabinose lipid A transferase; Lipid IV(A) 4-amino-4-deoxy-L-arabinosyltransferase; Undecaprenyl phosphate-alpha-L-Ara4N transferase [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MKTAFTVSSNREQSLFKDLIILAVVFGFAFFQRLGQIPLLDSDEGRYAEIPREMLERGDFITPFLNYVKYFEKPPLHYWL
EAISFRIFGFSEFAARAPGALMGLCGVLFTYHVGRTVFGRRAGLLAATVLGTSLGFIVIGRYNVIDMTLTFWMTATLGCF
LLAVHDRGRRAGLYYYLFYVAAALTVLAKGLIGLVLPGGVIVLFILLTGRWRLLREMRLPTGTVLFLLVAAPWFILVSLH
NPEFPRFFFIHEHFERFLTKVHGRYEPPWFFIPVLAGFMLPWSFYLPSAIRGMWQERHTPTGEARLYLILWGAVIFTFFS
LSSSKLIPYILPVFPAVALLVGEFFSRVADRRATFPTLPTAIAAAVLTIGGAGGILFPHLAKNPKLSVLAGAVIGSCLLA
QGIAAFVGLRRKKAAVLLVSFGLFSYLLGIAGPPFILARTAERKSFRELGQLVKEKAGPDTAVASFQLYQQGFSYYAGRR
IIIVGERGELDFGSRQGDQSQWFLDEQGFARLWDSGRPVIVLIKEDALPALRKLVTTPPRELGRKGKVLLIANH

Sequences:

>Translated_554_residues
MKTAFTVSSNREQSLFKDLIILAVVFGFAFFQRLGQIPLLDSDEGRYAEIPREMLERGDFITPFLNYVKYFEKPPLHYWL
EAISFRIFGFSEFAARAPGALMGLCGVLFTYHVGRTVFGRRAGLLAATVLGTSLGFIVIGRYNVIDMTLTFWMTATLGCF
LLAVHDRGRRAGLYYYLFYVAAALTVLAKGLIGLVLPGGVIVLFILLTGRWRLLREMRLPTGTVLFLLVAAPWFILVSLH
NPEFPRFFFIHEHFERFLTKVHGRYEPPWFFIPVLAGFMLPWSFYLPSAIRGMWQERHTPTGEARLYLILWGAVIFTFFS
LSSSKLIPYILPVFPAVALLVGEFFSRVADRRATFPTLPTAIAAAVLTIGGAGGILFPHLAKNPKLSVLAGAVIGSCLLA
QGIAAFVGLRRKKAAVLLVSFGLFSYLLGIAGPPFILARTAERKSFRELGQLVKEKAGPDTAVASFQLYQQGFSYYAGRR
IIIVGERGELDFGSRQGDQSQWFLDEQGFARLWDSGRPVIVLIKEDALPALRKLVTTPPRELGRKGKVLLIANH
>Mature_554_residues
MKTAFTVSSNREQSLFKDLIILAVVFGFAFFQRLGQIPLLDSDEGRYAEIPREMLERGDFITPFLNYVKYFEKPPLHYWL
EAISFRIFGFSEFAARAPGALMGLCGVLFTYHVGRTVFGRRAGLLAATVLGTSLGFIVIGRYNVIDMTLTFWMTATLGCF
LLAVHDRGRRAGLYYYLFYVAAALTVLAKGLIGLVLPGGVIVLFILLTGRWRLLREMRLPTGTVLFLLVAAPWFILVSLH
NPEFPRFFFIHEHFERFLTKVHGRYEPPWFFIPVLAGFMLPWSFYLPSAIRGMWQERHTPTGEARLYLILWGAVIFTFFS
LSSSKLIPYILPVFPAVALLVGEFFSRVADRRATFPTLPTAIAAAVLTIGGAGGILFPHLAKNPKLSVLAGAVIGSCLLA
QGIAAFVGLRRKKAAVLLVSFGLFSYLLGIAGPPFILARTAERKSFRELGQLVKEKAGPDTAVASFQLYQQGFSYYAGRR
IIIVGERGELDFGSRQGDQSQWFLDEQGFARLWDSGRPVIVLIKEDALPALRKLVTTPPRELGRKGKVLLIANH

Specific function: Catalyzes the transfer of the L-Ara4N moiety of the glycolipid undecaprenyl phosphate-alpha-L-Ara4N to lipid A. The modified arabinose is attached to lipid A and is required for resistance to polymyxin and cationic antimicrobial peptides [H]

COG id: COG1807

COG function: function code M; 4-amino-4-deoxy-L-arabinose transferase and related glycosyltransferases of PMT family

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 83 family [H]

Homologues:

Organism=Escherichia coli, GI1788591, Length=533, Percent_Identity=27.3921200750469, Blast_Score=155, Evalue=9e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022839
- InterPro:   IPR003342 [H]

Pfam domain/function: PF02366 PMT [H]

EC number: =2.4.2.43 [H]

Molecular weight: Translated: 61868; Mature: 61868

Theoretical pI: Translated: 10.43; Mature: 10.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTAFTVSSNREQSLFKDLIILAVVFGFAFFQRLGQIPLLDSDEGRYAEIPREMLERGDF
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCC
ITPFLNYVKYFEKPPLHYWLEAISFRIFGFSEFAARAPGALMGLCGVLFTYHVGRTVFGR
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RAGLLAATVLGTSLGFIVIGRYNVIDMTLTFWMTATLGCFLLAVHDRGRRAGLYYYLFYV
HHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AAALTVLAKGLIGLVLPGGVIVLFILLTGRWRLLREMRLPTGTVLFLLVAAPWFILVSLH
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHCCHHEEEEEC
NPEFPRFFFIHEHFERFLTKVHGRYEPPWFFIPVLAGFMLPWSFYLPSAIRGMWQERHTP
CCCCCEEEHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
TGEARLYLILWGAVIFTFFSLSSSKLIPYILPVFPAVALLVGEFFSRVADRRATFPTLPT
CCCCEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
AIAAAVLTIGGAGGILFPHLAKNPKLSVLAGAVIGSCLLAQGIAAFVGLRRKKAAVLLVS
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGLFSYLLGIAGPPFILARTAERKSFRELGQLVKEKAGPDTAVASFQLYQQGFSYYAGRR
HHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCE
IIIVGERGELDFGSRQGDQSQWFLDEQGFARLWDSGRPVIVLIKEDALPALRKLVTTPPR
EEEEECCCCCCCCCCCCCCCCEEECCCHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCH
ELGRKGKVLLIANH
HHCCCCCEEEEECC
>Mature Secondary Structure
MKTAFTVSSNREQSLFKDLIILAVVFGFAFFQRLGQIPLLDSDEGRYAEIPREMLERGDF
CCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCC
ITPFLNYVKYFEKPPLHYWLEAISFRIFGFSEFAARAPGALMGLCGVLFTYHVGRTVFGR
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
RAGLLAATVLGTSLGFIVIGRYNVIDMTLTFWMTATLGCFLLAVHDRGRRAGLYYYLFYV
HHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
AAALTVLAKGLIGLVLPGGVIVLFILLTGRWRLLREMRLPTGTVLFLLVAAPWFILVSLH
HHHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHCCHHEEEEEC
NPEFPRFFFIHEHFERFLTKVHGRYEPPWFFIPVLAGFMLPWSFYLPSAIRGMWQERHTP
CCCCCEEEHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
TGEARLYLILWGAVIFTFFSLSSSKLIPYILPVFPAVALLVGEFFSRVADRRATFPTLPT
CCCCEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
AIAAAVLTIGGAGGILFPHLAKNPKLSVLAGAVIGSCLLAQGIAAFVGLRRKKAAVLLVS
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FGLFSYLLGIAGPPFILARTAERKSFRELGQLVKEKAGPDTAVASFQLYQQGFSYYAGRR
HHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCE
IIIVGERGELDFGSRQGDQSQWFLDEQGFARLWDSGRPVIVLIKEDALPALRKLVTTPPR
EEEEECCCCCCCCCCCCCCCCEEECCCHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCH
ELGRKGKVLLIANH
HHCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA