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The map label for this gene is recJ [H]

Identifier: 78222077

GI number: 78222077

Start: 943526

End: 945262

Strand: Direct

Name: recJ [H]

Synonym: Gmet_0857

Alternate gene names: 78222077

Gene position: 943526-945262 (Clockwise)

Preceding gene: 78222076

Following gene: 78222078

Centisome position: 23.6

GC content: 60.33

Gene sequence:

>1737_bases
ATGAAAGCGGTTGCCGAAAAGCGATGGGAGTTACGGACCAATGACAAGGAGGCTGCGCAGAGAATTGCCTCGGACTCTTC
GGTCGACCCCTTCCTGGCAGCGCTGCTCGCCAACCGGGGCATCGCGGATGGCGAATCCGCGCGCCGCTTTCTTTCCTCTT
CCCTGGGGGACGTGAGCGATCCCTTCCTTCTGAAGGGAATGGAAGAGGCAGTTGCCCGCCTCTGTGCGGCACGGGCACGA
CGTGAAACTGTGTGCGTCTACGGCGATTATGATGTGGATGGGATTACTGCCACGGCATTGCTGGTAAGCTTTTTTCGTGC
GGTTGGTATCCGCTGCTTCTATCATATTCCGAAACGCCTCGAAGAGGGATACGGCCTTTCGGTGGACGGCCTGCGCAAGG
TGGCCGAGCAGGCGCAGGTTGTCGTTACGGTCGACTGTGGGGTGAATGCCGTGGCCGAGGCGGAACTCTGCCTTACGTTA
GGGGTTGACCTCGTCATCACCGACCACCACACCCCTGGTCCGGCCATCCCGAAAGCCTGTGCCGTTATCAATCCACTTCA
GCCGGGCTGTTCCTATCCCTTTAAATCCCTTGCAGGCGTGGGCGTCGCTTTTAACGTGATGGTCGCCCTTCGGGGGCGTC
TCAGGGCGGAAGGGGCATTCGCCGGAGGAAACGGACCGAATCTGCGGGAATACCTCGACCTCGTCGCCCTCGGAACCATT
GCCGATGTGGTTCCTCTCGTGGGAGAGAACCGGATATTCGTTAAATATGGGCTCCGGGAACTTTCCGCTTCGTCGCGGGT
TGGAGTGAAGGCCCTTAAGGATGTGGCGGGAGTCACCGGCGCCGTGAGTTGTGGTGCTGTCGGCTTCAGGCTTGCACCGC
GACTTAACGCAGCCGGCAGAATGGAGGATGCCGCTGCCGGGGTTGAACTTCTCCTTGAGACCGAGCCTGGCCGTGCCGCG
GCCATTGCCGCGGAACTGGATGCGAATAACGACGAGCGCCAGGCCCTGGAGCGGCAGATTCTGACGGACGCCCTGGGACG
GGTTCAAGGGAATACCGCCATGGAGGGGAGAAAAAGTATCGTTCTCGCCTCCGCTGCGTGGCATCCGGGGGTCATCGGGA
TCGTTGCCTCGCGAATGGTCGATCTCTTCCATCGTCCGACCGTGCTCATTGCTCTGCAGGAAGGAAACGGCCGGGGATCG
GGGCGCAGTATTCCGGGCTTTCACCTCTACGATGCCCTCGGCGCCTGCGCCGACCAACTCGTGAAGTTCGGCGGGCACCG
TTACGCTGCCGGGCTTTCAATTGATGAGCCAACGCTGGAACTGTTCGTGGACCGCTTCGAAGAAGTTGCTGCGGGGTTGC
TGTCCGCCGACGACCTGATACCGGTGCTCCGCGTGGATGCTGTACTCTCGGCCCAGCAGGTGACAGCTGGTCTGGCGGAC
GCGCTTTCGTCCCTGGAGCCCTTCGGCGCGGGAAATCCCGAACCGCTGTTCGTGCTGAAAGATATGGAGATCGTGCGGCG
GTGGGCCGTTCGCGGCAACCATCTCAAAGTACGGCTTTCTGGTGGAGGATTCACTTTTGACGCCATTGGTTTTAATCTTG
CAACGAAGGAGGACAGCTTGCCAGATCTCGTGGATATCGTTTTTACGCTCGGCTGGAACGAGTGGAACGGCCGCAAAACC
CTGCAACTGAGCCTCAAGGACATCAGGGAGTCCGGTCAGGTGCTTGAGCCATGCTGA

Upstream 100 bases:

>100_bases
CCCCCCAGGGGCAACTCTTCAGGAAGTGATTTGACGGCATCGAATTCCCCGCTTCGGCGGGGTTTTTTTGTGGCCATTGT
GCGAGGTGGGGTGGCAGAGA

Downstream 100 bases:

>100_bases
GGGAAGAGCGTTTCAATAAAGCTGACCGTATTATCGCGGTCGGATTCTGGGCCAACGCAGGGCTCATGATCATGAAACTC
CTGGCCGGCTATTTCGGGAA

Product: RecJ exonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MKAVAEKRWELRTNDKEAAQRIASDSSVDPFLAALLANRGIADGESARRFLSSSLGDVSDPFLLKGMEEAVARLCAARAR
RETVCVYGDYDVDGITATALLVSFFRAVGIRCFYHIPKRLEEGYGLSVDGLRKVAEQAQVVVTVDCGVNAVAEAELCLTL
GVDLVITDHHTPGPAIPKACAVINPLQPGCSYPFKSLAGVGVAFNVMVALRGRLRAEGAFAGGNGPNLREYLDLVALGTI
ADVVPLVGENRIFVKYGLRELSASSRVGVKALKDVAGVTGAVSCGAVGFRLAPRLNAAGRMEDAAAGVELLLETEPGRAA
AIAAELDANNDERQALERQILTDALGRVQGNTAMEGRKSIVLASAAWHPGVIGIVASRMVDLFHRPTVLIALQEGNGRGS
GRSIPGFHLYDALGACADQLVKFGGHRYAAGLSIDEPTLELFVDRFEEVAAGLLSADDLIPVLRVDAVLSAQQVTAGLAD
ALSSLEPFGAGNPEPLFVLKDMEIVRRWAVRGNHLKVRLSGGGFTFDAIGFNLATKEDSLPDLVDIVFTLGWNEWNGRKT
LQLSLKDIRESGQVLEPC

Sequences:

>Translated_578_residues
MKAVAEKRWELRTNDKEAAQRIASDSSVDPFLAALLANRGIADGESARRFLSSSLGDVSDPFLLKGMEEAVARLCAARAR
RETVCVYGDYDVDGITATALLVSFFRAVGIRCFYHIPKRLEEGYGLSVDGLRKVAEQAQVVVTVDCGVNAVAEAELCLTL
GVDLVITDHHTPGPAIPKACAVINPLQPGCSYPFKSLAGVGVAFNVMVALRGRLRAEGAFAGGNGPNLREYLDLVALGTI
ADVVPLVGENRIFVKYGLRELSASSRVGVKALKDVAGVTGAVSCGAVGFRLAPRLNAAGRMEDAAAGVELLLETEPGRAA
AIAAELDANNDERQALERQILTDALGRVQGNTAMEGRKSIVLASAAWHPGVIGIVASRMVDLFHRPTVLIALQEGNGRGS
GRSIPGFHLYDALGACADQLVKFGGHRYAAGLSIDEPTLELFVDRFEEVAAGLLSADDLIPVLRVDAVLSAQQVTAGLAD
ALSSLEPFGAGNPEPLFVLKDMEIVRRWAVRGNHLKVRLSGGGFTFDAIGFNLATKEDSLPDLVDIVFTLGWNEWNGRKT
LQLSLKDIRESGQVLEPC
>Mature_578_residues
MKAVAEKRWELRTNDKEAAQRIASDSSVDPFLAALLANRGIADGESARRFLSSSLGDVSDPFLLKGMEEAVARLCAARAR
RETVCVYGDYDVDGITATALLVSFFRAVGIRCFYHIPKRLEEGYGLSVDGLRKVAEQAQVVVTVDCGVNAVAEAELCLTL
GVDLVITDHHTPGPAIPKACAVINPLQPGCSYPFKSLAGVGVAFNVMVALRGRLRAEGAFAGGNGPNLREYLDLVALGTI
ADVVPLVGENRIFVKYGLRELSASSRVGVKALKDVAGVTGAVSCGAVGFRLAPRLNAAGRMEDAAAGVELLLETEPGRAA
AIAAELDANNDERQALERQILTDALGRVQGNTAMEGRKSIVLASAAWHPGVIGIVASRMVDLFHRPTVLIALQEGNGRGS
GRSIPGFHLYDALGACADQLVKFGGHRYAAGLSIDEPTLELFVDRFEEVAAGLLSADDLIPVLRVDAVLSAQQVTAGLAD
ALSSLEPFGAGNPEPLFVLKDMEIVRRWAVRGNHLKVRLSGGGFTFDAIGFNLATKEDSLPDLVDIVFTLGWNEWNGRKT
LQLSLKDIRESGQVLEPC

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=563, Percent_Identity=39.4316163410302, Blast_Score=323, Evalue=2e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 61507; Mature: 61507

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVAEKRWELRTNDKEAAQRIASDSSVDPFLAALLANRGIADGESARRFLSSSLGDVSD
CCCHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCC
PFLLKGMEEAVARLCAARARRETVCVYGDYDVDGITATALLVSFFRAVGIRCFYHIPKRL
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEGYGLSVDGLRKVAEQAQVVVTVDCGVNAVAEAELCLTLGVDLVITDHHTPGPAIPKAC
HCCCCCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHEEECCEEEEECCCCCCCCCHHHH
AVINPLQPGCSYPFKSLAGVGVAFNVMVALRGRLRAEGAFAGGNGPNLREYLDLVALGTI
HHCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
ADVVPLVGENRIFVKYGLRELSASSRVGVKALKDVAGVTGAVSCGAVGFRLAPRLNAAGR
HHHHHHCCCCEEEEEECHHHHCCCCCHHHHHHHHHHCCCCCHHHCCCCEEECCCCCCCCC
MEDAAAGVELLLETEPGRAAAIAAELDANNDERQALERQILTDALGRVQGNTAMEGRKSI
CHHHHCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
VLASAAWHPGVIGIVASRMVDLFHRPTVLIALQEGNGRGSGRSIPGFHLYDALGACADQL
EEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
VKFGGHRYAAGLSIDEPTLELFVDRFEEVAAGLLSADDLIPVLRVDAVLSAQQVTAGLAD
HHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ALSSLEPFGAGNPEPLFVLKDMEIVRRWAVRGNHLKVRLSGGGFTFDAIGFNLATKEDSL
HHHHCCCCCCCCCCCEEEECCHHHHHHHHCCCCEEEEEECCCCEEEEECCCEEECCCCCC
PDLVDIVFTLGWNEWNGRKTLQLSLKDIRESGQVLEPC
HHHHHHHHHHCCCCCCCCEEEEEEHHHHHHCCCCCCCC
>Mature Secondary Structure
MKAVAEKRWELRTNDKEAAQRIASDSSVDPFLAALLANRGIADGESARRFLSSSLGDVSD
CCCHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCC
PFLLKGMEEAVARLCAARARRETVCVYGDYDVDGITATALLVSFFRAVGIRCFYHIPKRL
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEGYGLSVDGLRKVAEQAQVVVTVDCGVNAVAEAELCLTLGVDLVITDHHTPGPAIPKAC
HCCCCCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHEEECCEEEEECCCCCCCCCHHHH
AVINPLQPGCSYPFKSLAGVGVAFNVMVALRGRLRAEGAFAGGNGPNLREYLDLVALGTI
HHCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHH
ADVVPLVGENRIFVKYGLRELSASSRVGVKALKDVAGVTGAVSCGAVGFRLAPRLNAAGR
HHHHHHCCCCEEEEEECHHHHCCCCCHHHHHHHHHHCCCCCHHHCCCCEEECCCCCCCCC
MEDAAAGVELLLETEPGRAAAIAAELDANNDERQALERQILTDALGRVQGNTAMEGRKSI
CHHHHCCEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
VLASAAWHPGVIGIVASRMVDLFHRPTVLIALQEGNGRGSGRSIPGFHLYDALGACADQL
EEEECCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHH
VKFGGHRYAAGLSIDEPTLELFVDRFEEVAAGLLSADDLIPVLRVDAVLSAQQVTAGLAD
HHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ALSSLEPFGAGNPEPLFVLKDMEIVRRWAVRGNHLKVRLSGGGFTFDAIGFNLATKEDSL
HHHHCCCCCCCCCCCEEEECCHHHHHHHHCCCCEEEEEECCCCEEEEECCCEEECCCCCC
PDLVDIVFTLGWNEWNGRKTLQLSLKDIRESGQVLEPC
HHHHHHHHHHCCCCCCCCEEEEEEHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]