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The map label for this gene is bioH [H]

Identifier: 78222063

GI number: 78222063

Start: 928693

End: 929511

Strand: Direct

Name: bioH [H]

Synonym: Gmet_0843

Alternate gene names: 78222063

Gene position: 928693-929511 (Clockwise)

Preceding gene: 78222062

Following gene: 78222064

Centisome position: 23.23

GC content: 66.54

Gene sequence:

>819_bases
ATGCCCTTTGTGGAAACCGCTCAGAAGGTAACGATCCACTATGAGGACGAAGGTTCGGGCTTTCCCCTTGTCTTCGTCCA
TGGCTGGGCCATGTCCGGGAAGGTCTGGGCCTTCCAGAAGCCCCTGGCTGACCATTTTCGCCTCATTACGTTGGACCTTC
GGGGGCACGGAAAATCGTCCGCTGCTCCCGGTTACGCCTTTTCCGACTTCGCCGCCGACCTGGCAGCCCTCTTCGACCGG
TTGGGACTCGAGCGCGCTGCACTTGTCGGCTGGTCCCTGGGGGCCCAGGTGGCCCTGGAGGCCGTTCCGCTCCTGGAGGA
CCGCGTTGCTGCCCTGGTGCTCGTGGCCGGCACCCCCAAGTTCACCACCGCAGACGGCTGGCCCCACGGCCTCCCGCCAC
ATGAGGCACGAGGGCTCGGCCTGCGGCTGAAGCGGGCCTATGACGCCACCCTGGGGGACTTTTTCCGGCAGATGTTCGCG
GAGGGGGAGCTGTCCCACGATCAGTATCAGCGCATCGTCCGGGAGATCGTGATTCCCCGGCACCTCCCCGATCCCGGCGC
GGTGCAGGCATGCCTCGTCACCCTCGCCGGCGGCGACCACCGCAATCTCCTGCCCGCTATCGCCGCGCCGACCCTCGTCA
TCCACGGCGACCGGGATGCCATCTGTTCCCCCAGTGCTGGCCGGTACCTGGCCGAGGGGATTCCCGGCGCCCGCTTCCTC
TCCCTGGAGGGGACAGGGCATGCCCCGTTCCTCTCGCAGCCAGAGCGGTTCAACCAGGAGCTGAGCCGCTTCCTTACGGA
GGTGATCGACCGTGATTGA

Upstream 100 bases:

>100_bases
GCTCCGTTGCACTCTCATGGCGACCCATGCAGAGGAGGACCTGGCTCGGGCAGTGGCGCTCATGGCGGCAATTGGCAAAG
GGATGGGGGTAATCTGACCG

Downstream 100 bases:

>100_bases
CCGCCGCAGGGTGAGACATTCCTTCCACCGGCAGGCCCTGGAGTACGACGCCCATGCAGCTGTCCAGAAACGGGTCGTGG
AACGGTTTCTCCAGATCTTG

Product: Alpha/beta hydrolase fold

Products: NA

Alternate protein names: Biotin synthesis protein BioH [H]

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MPFVETAQKVTIHYEDEGSGFPLVFVHGWAMSGKVWAFQKPLADHFRLITLDLRGHGKSSAAPGYAFSDFAADLAALFDR
LGLERAALVGWSLGAQVALEAVPLLEDRVAALVLVAGTPKFTTADGWPHGLPPHEARGLGLRLKRAYDATLGDFFRQMFA
EGELSHDQYQRIVREIVIPRHLPDPGAVQACLVTLAGGDHRNLLPAIAAPTLVIHGDRDAICSPSAGRYLAEGIPGARFL
SLEGTGHAPFLSQPERFNQELSRFLTEVIDRD

Sequences:

>Translated_272_residues
MPFVETAQKVTIHYEDEGSGFPLVFVHGWAMSGKVWAFQKPLADHFRLITLDLRGHGKSSAAPGYAFSDFAADLAALFDR
LGLERAALVGWSLGAQVALEAVPLLEDRVAALVLVAGTPKFTTADGWPHGLPPHEARGLGLRLKRAYDATLGDFFRQMFA
EGELSHDQYQRIVREIVIPRHLPDPGAVQACLVTLAGGDHRNLLPAIAAPTLVIHGDRDAICSPSAGRYLAEGIPGARFL
SLEGTGHAPFLSQPERFNQELSRFLTEVIDRD
>Mature_271_residues
PFVETAQKVTIHYEDEGSGFPLVFVHGWAMSGKVWAFQKPLADHFRLITLDLRGHGKSSAAPGYAFSDFAADLAALFDRL
GLERAALVGWSLGAQVALEAVPLLEDRVAALVLVAGTPKFTTADGWPHGLPPHEARGLGLRLKRAYDATLGDFFRQMFAE
GELSHDQYQRIVREIVIPRHLPDPGAVQACLVTLAGGDHRNLLPAIAAPTLVIHGDRDAICSPSAGRYLAEGIPGARFLS
LEGTGHAPFLSQPERFNQELSRFLTEVIDRD

Specific function: Shows carboxylesterase activity with a preference for short chain fatty acid esters (acyl chain length of up to 6 carbons). Also displays a weak thioesterase activity. Can form a complex with CoA, and may be involved in the condensation of CoA and pimelic

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family [H]

Homologues:

Organism=Escherichia coli, GI1789817, Length=249, Percent_Identity=28.5140562248996, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010076 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 29594; Mature: 29463

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFVETAQKVTIHYEDEGSGFPLVFVHGWAMSGKVWAFQKPLADHFRLITLDLRGHGKSS
CCCCCCCEEEEEEECCCCCCCCEEEEEEEEECCEEEEECCCCCCCEEEEEEEECCCCCCC
AAPGYAFSDFAADLAALFDRLGLERAALVGWSLGAQVALEAVPLLEDRVAALVLVAGTPK
CCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEEEECCCC
FTTADGWPHGLPPHEARGLGLRLKRAYDATLGDFFRQMFAEGELSHDQYQRIVREIVIPR
CCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
HLPDPGAVQACLVTLAGGDHRNLLPAIAAPTLVIHGDRDAICSPSAGRYLAEGIPGARFL
CCCCCHHHHHHHHHCCCCCCCCCCHHHCCCEEEEECCCCCCCCCCCCCHHHHCCCCCEEE
SLEGTGHAPFLSQPERFNQELSRFLTEVIDRD
EECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PFVETAQKVTIHYEDEGSGFPLVFVHGWAMSGKVWAFQKPLADHFRLITLDLRGHGKSS
CCCCCCEEEEEEECCCCCCCCEEEEEEEEECCEEEEECCCCCCCEEEEEEEECCCCCCC
AAPGYAFSDFAADLAALFDRLGLERAALVGWSLGAQVALEAVPLLEDRVAALVLVAGTPK
CCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEEEECCCC
FTTADGWPHGLPPHEARGLGLRLKRAYDATLGDFFRQMFAEGELSHDQYQRIVREIVIPR
CCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
HLPDPGAVQACLVTLAGGDHRNLLPAIAAPTLVIHGDRDAICSPSAGRYLAEGIPGARFL
CCCCCHHHHHHHHHCCCCCCCCCCHHHCCCEEEEECCCCCCCCCCCCCHHHHCCCCCEEE
SLEGTGHAPFLSQPERFNQELSRFLTEVIDRD
EECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]