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The map label for this gene is bioF

Identifier: 78222062

GI number: 78222062

Start: 927499

End: 928689

Strand: Direct

Name: bioF

Synonym: Gmet_0842

Alternate gene names: 78222062

Gene position: 927499-928689 (Clockwise)

Preceding gene: 78222055

Following gene: 78222063

Centisome position: 23.2

GC content: 67.67

Gene sequence:

>1191_bases
ATGAAAGGAGAACCCGTGTCGAAAAGCTTTGCCGAAGAACTGGAGATGCTGAAGGCCCAGGGGCTTCACCGGAGGATGCG
CCGCATCGCCGGAAGCCAGGGGAGCAGGGCCGTTGTGGACGGGAAGGAGGCTCTCCTCCTCTGCTCCAACAACTACCTGG
GGCTGGCCGACCATCCCCGCCTGGCGGAGGCGGCCATCCGGGCCGTGGAGCGCTACGGCACCTCCAGCGGCGCATCGCGT
CTCGTCTCCGGCACCATGGAACTCCACGCCCTCCTCGAAGAGCGGATCGCCCGCTTCAAGGGGACCGGCGCGGCCCTGGT
CTTCAACTCGGGCTATGCCGCCAACAGCGGCATCATCCCGGCCCTGGTGGGGAAGGGGGACGTGGTCTTCTCCGACCGGC
TCAACCACGCGAGCATCGTGGATGGCTGCCTCCTCTCCCGGGCCACCATGGTCCGCTATCCCCATAACGACACGGCTGCC
CTGCGGCGGCTTATGGAGCGGCACGAAACCGCGGGCCGGCGGCTCCTCGTCACCGACGGGGTCTTCAGCATGGACGGTGA
CATGGCGCCGCTGCGGGAGCTGGCGGCCCTCAAGCGGGAATTCGGCGCCCTCCTCATGGTGGACGATGCCCACGGCACCG
GCGTTCTGGGCGCGACCGGCCGGGGGAGCGCCGAGCTTCAGGGGGTGATGGCGGAGATCGACATCCACATGGGAACCCTC
GGCAAGGCCCTCGGCAGTTTCGGCGCCTACGCCGCGGCTTCGGAGGACGTGATCGATTACCTGGCGAACAAGGCCCGCAG
TTTCATCTTTTCCACGTCGCTCCCACCGGCGGTCCTGGCCGCATCCCTTGCCGCCGTCGATCTGGTCGACTCCCCCGATG
GCGCGGCGCTCCGGGAGCGGCTTGCCCGCAACACCGCTTTTTTCAGAGAGGGCTTGTGCGCCGCCGGTTTCGACACCATG
GGGAGCGAGACCCAGATCGTTCCCCTCTTCGTGGGGGGAGCCGAAGAGACCATGGCCTTCACGGCCCGGTTGCTGGAGGC
AGGGGTCTTTGCCCAGGGGATACGCCCTCCCACGGTCCCGGCCGGGACCTGCCGGCTCCGTTGCACTCTCATGGCGACCC
ATGCAGAGGAGGACCTGGCTCGGGCAGTGGCGCTCATGGCGGCAATTGGCAAAGGGATGGGGGTAATCTGA

Upstream 100 bases:

>100_bases
ACCATGCGGACCCCTGTCAAGTAAACGGTCACTGATTGTCAACCTTTGTGTCTGGCTTGGTTGACGAAAAGGCGGCGAAT
GTGCTACAAACGGAGCGCCG

Downstream 100 bases:

>100_bases
CCGATGCCCTTTGTGGAAACCGCTCAGAAGGTAACGATCCACTATGAGGACGAAGGTTCGGGCTTTCCCCTTGTCTTCGT
CCATGGCTGGGCCATGTCCG

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MKGEPVSKSFAEELEMLKAQGLHRRMRRIAGSQGSRAVVDGKEALLLCSNNYLGLADHPRLAEAAIRAVERYGTSSGASR
LVSGTMELHALLEERIARFKGTGAALVFNSGYAANSGIIPALVGKGDVVFSDRLNHASIVDGCLLSRATMVRYPHNDTAA
LRRLMERHETAGRRLLVTDGVFSMDGDMAPLRELAALKREFGALLMVDDAHGTGVLGATGRGSAELQGVMAEIDIHMGTL
GKALGSFGAYAAASEDVIDYLANKARSFIFSTSLPPAVLAASLAAVDLVDSPDGAALRERLARNTAFFREGLCAAGFDTM
GSETQIVPLFVGGAEETMAFTARLLEAGVFAQGIRPPTVPAGTCRLRCTLMATHAEEDLARAVALMAAIGKGMGVI

Sequences:

>Translated_396_residues
MKGEPVSKSFAEELEMLKAQGLHRRMRRIAGSQGSRAVVDGKEALLLCSNNYLGLADHPRLAEAAIRAVERYGTSSGASR
LVSGTMELHALLEERIARFKGTGAALVFNSGYAANSGIIPALVGKGDVVFSDRLNHASIVDGCLLSRATMVRYPHNDTAA
LRRLMERHETAGRRLLVTDGVFSMDGDMAPLRELAALKREFGALLMVDDAHGTGVLGATGRGSAELQGVMAEIDIHMGTL
GKALGSFGAYAAASEDVIDYLANKARSFIFSTSLPPAVLAASLAAVDLVDSPDGAALRERLARNTAFFREGLCAAGFDTM
GSETQIVPLFVGGAEETMAFTARLLEAGVFAQGIRPPTVPAGTCRLRCTLMATHAEEDLARAVALMAAIGKGMGVI
>Mature_396_residues
MKGEPVSKSFAEELEMLKAQGLHRRMRRIAGSQGSRAVVDGKEALLLCSNNYLGLADHPRLAEAAIRAVERYGTSSGASR
LVSGTMELHALLEERIARFKGTGAALVFNSGYAANSGIIPALVGKGDVVFSDRLNHASIVDGCLLSRATMVRYPHNDTAA
LRRLMERHETAGRRLLVTDGVFSMDGDMAPLRELAALKREFGALLMVDDAHGTGVLGATGRGSAELQGVMAEIDIHMGTL
GKALGSFGAYAAASEDVIDYLANKARSFIFSTSLPPAVLAASLAAVDLVDSPDGAALRERLARNTAFFREGLCAAGFDTM
GSETQIVPLFVGGAEETMAFTARLLEAGVFAQGIRPPTVPAGTCRLRCTLMATHAEEDLARAVALMAAIGKGMGVI

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily

Homologues:

Organism=Homo sapiens, GI7657118, Length=387, Percent_Identity=39.5348837209302, Blast_Score=275, Evalue=5e-74,
Organism=Homo sapiens, GI284448556, Length=413, Percent_Identity=37.046004842615, Blast_Score=265, Evalue=5e-71,
Organism=Homo sapiens, GI83977442, Length=346, Percent_Identity=36.1271676300578, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI83977440, Length=346, Percent_Identity=36.1271676300578, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI83977444, Length=346, Percent_Identity=36.1271676300578, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI4502025, Length=364, Percent_Identity=34.6153846153846, Blast_Score=222, Evalue=6e-58,
Organism=Homo sapiens, GI40316939, Length=364, Percent_Identity=34.6153846153846, Blast_Score=222, Evalue=6e-58,
Organism=Homo sapiens, GI4758668, Length=358, Percent_Identity=35.7541899441341, Blast_Score=206, Evalue=3e-53,
Organism=Homo sapiens, GI119220554, Length=365, Percent_Identity=34.5205479452055, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI5454084, Length=396, Percent_Identity=29.7979797979798, Blast_Score=166, Evalue=4e-41,
Organism=Escherichia coli, GI1790046, Length=400, Percent_Identity=40.75, Blast_Score=294, Evalue=6e-81,
Organism=Escherichia coli, GI1786993, Length=358, Percent_Identity=41.8994413407821, Blast_Score=254, Evalue=9e-69,
Organism=Caenorhabditis elegans, GI71994529, Length=390, Percent_Identity=37.1794871794872, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI32566772, Length=368, Percent_Identity=32.8804347826087, Blast_Score=191, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17560912, Length=358, Percent_Identity=30.1675977653631, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17560914, Length=358, Percent_Identity=30.1675977653631, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71982617, Length=312, Percent_Identity=28.8461538461538, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71982625, Length=312, Percent_Identity=28.8461538461538, Blast_Score=139, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320438, Length=368, Percent_Identity=30.7065217391304, Blast_Score=183, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6320267, Length=365, Percent_Identity=31.2328767123288, Blast_Score=181, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6323954, Length=261, Percent_Identity=29.8850574712644, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24662918, Length=387, Percent_Identity=38.7596899224806, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI17137420, Length=389, Percent_Identity=31.8766066838046, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI17136286, Length=387, Percent_Identity=31.7829457364341, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24653280, Length=277, Percent_Identity=32.129963898917, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24653276, Length=277, Percent_Identity=32.129963898917, Blast_Score=151, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24653278, Length=277, Percent_Identity=32.129963898917, Blast_Score=151, Evalue=7e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_GEOMG (Q39XE0)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383809.1
- HSSP:   P12998
- ProteinModelPortal:   Q39XE0
- STRING:   Q39XE0
- GeneID:   3738296
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0842
- NMPDR:   fig|269799.3.peg.906
- eggNOG:   COG0156
- HOGENOM:   HBG649839
- OMA:   QGIRPPT
- PhylomeDB:   Q39XE0
- ProtClustDB:   CLSK828920
- BioCyc:   GMET269799:GMET_0842-MONOMER
- HAMAP:   MF_01693
- InterPro:   IPR022834
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 41750; Mature: 41750

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 24-24 BINDING 136-136 BINDING 183-183 BINDING 358-358

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGEPVSKSFAEELEMLKAQGLHRRMRRIAGSQGSRAVVDGKEALLLCSNNYLGLADHPR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCEECCCCCCH
LAEAAIRAVERYGTSSGASRLVSGTMELHALLEERIARFKGTGAALVFNSGYAANSGIIP
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCE
ALVGKGDVVFSDRLNHASIVDGCLLSRATMVRYPHNDTAALRRLMERHETAGRRLLVTDG
EEECCCCEEEECCCCCHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCEEEEECC
VFSMDGDMAPLRELAALKREFGALLMVDDAHGTGVLGATGRGSAELQGVMAEIDIHMGTL
CEECCCCHHHHHHHHHHHHHHCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHH
GKALGSFGAYAAASEDVIDYLANKARSFIFSTSLPPAVLAASLAAVDLVDSPDGAALRER
HHHHHHHCCHHCCHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHEECCCCCCHHHHHH
LARNTAFFREGLCAAGFDTMGSETQIVPLFVGGAEETMAFTARLLEAGVFAQGIRPPTVP
HHHHHHHHHCCCHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCC
AGTCRLRCTLMATHAEEDLARAVALMAAIGKGMGVI
CCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKGEPVSKSFAEELEMLKAQGLHRRMRRIAGSQGSRAVVDGKEALLLCSNNYLGLADHPR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCCEECCCCCCH
LAEAAIRAVERYGTSSGASRLVSGTMELHALLEERIARFKGTGAALVFNSGYAANSGIIP
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCE
ALVGKGDVVFSDRLNHASIVDGCLLSRATMVRYPHNDTAALRRLMERHETAGRRLLVTDG
EEECCCCEEEECCCCCHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHCCCEEEEECC
VFSMDGDMAPLRELAALKREFGALLMVDDAHGTGVLGATGRGSAELQGVMAEIDIHMGTL
CEECCCCHHHHHHHHHHHHHHCCEEEEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHH
GKALGSFGAYAAASEDVIDYLANKARSFIFSTSLPPAVLAASLAAVDLVDSPDGAALRER
HHHHHHHCCHHCCHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHEECCCCCCHHHHHH
LARNTAFFREGLCAAGFDTMGSETQIVPLFVGGAEETMAFTARLLEAGVFAQGIRPPTVP
HHHHHHHHHCCCHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCC
AGTCRLRCTLMATHAEEDLARAVALMAAIGKGMGVI
CCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA