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The map label for this gene is amy [H]

Identifier: 78222053

GI number: 78222053

Start: 918513

End: 920366

Strand: Direct

Name: amy [H]

Synonym: Gmet_0833

Alternate gene names: 78222053

Gene position: 918513-920366 (Clockwise)

Preceding gene: 78222052

Following gene: 78222054

Centisome position: 22.98

GC content: 63.92

Gene sequence:

>1854_bases
ATGACGGTACCGCAATCGATCACGGAACTGGATCTCAAGAACCTCACGAATCGCACGTTTCACCCTTCGCCCGTGGCCTG
GGAAGACCAGGTGCTCTACTTTCTCCTGCTCGACCGCTTTTCCGACGGGAAGGAGACCGGATACCGCGACAACCGGGGGA
GGCGGGTCGCGAAGGGGGAAACGCCCCTTTACCAGCCAGGCGACGCGGGGAATGCCATCCGCACCGAGCCCGAGGCTGCC
GTCTGGCGCGAGTCGGGGAGCCGCTGGTGCGGCGGAACCCTGAAGGGGCTAGCCAGCAAGATGGGGTATCTCAAGCGCAT
GGGAGTGACCGCCGTCTGGGTCAGCCCCCTCTTCAAGCAGTGCTCCTTCGTCCCTACCTACCACGGCTACGGCATCCAGA
ACTTCCTCGAGGTGGACCCCCATTTCGGCACCGCCGACGACCTGCGGGAACTGGTCCATGTTGCCCATGCCAACGGCATC
TACGTCATCCTCGACATCATCCTGAACCACGCCGGCAATATCTTTGCCTACGACCGGGGCTATACCCCCTATGACGGCTC
CACCGTCTACCCGGTGACGGGGTTTCGCTCCGCCGACGGCACGCCGAACCTCCCCTTCGGCCCCATCGATACCAAGGCCA
ACCCCGATGCCTGGCCCGATGGCGCGGTCTGGCCCCGGGAGCTTCAGGCGGCCAACGCCTATACCCGCATGGGCCACATC
CGGGATTGGGACAGCTACCACGAATACCTGGACGGTGACTTCTTCGACCTGAAGGACATCCACCTGGGTGAGGGCGAGGT
TGATGCTTTCCGTCCGTCGCCGGCACTCCTGGCCCTCTGCGAGGTTTACAAGTACTGGATTGCCCTCTCCGATGTGGACG
GATACCGCATCGACACGGTGAAGCACATGGGTGCCGGGGCCACCCGGTTTTTCGCCTCGGTGATCCACGAGTTCGCCCAG
GCCATCGGCAAGGAGAACTTTTACCTCATCGGCGAGATCACCGGCGGCCGCGACAATGCCTTCAACACCCTGGCGACCAC
CGGCCTCAACGCGGCACTCGGCGTGGACGACATTCCGCTGCGCCTTGAGGACCTGGTGAAAGGGAGGGCAAACCCCGCCG
ACTACTTCAACCTCTTCCGCAACTCCCTCCTGGTGCGGAAGGAGTCCCACGTCTGGTTCCGGGACAAGGTGGTGACGGTC
ATCGACGACCACGACCAGGTCTGTCGGGGGGCCAAGAAGGAGCGTTTCTGCGCCGGCGATCCCAAGTGGCGCCGGCTCGT
GCTGAACGCCCTGGCCCTGAACACGACCACCCTCGGCATCCCCTGCGTCTACTATGGCACCGAGCAGGCCTTTGACGGCG
AGGGGGGCGATGACCGGTATCTGCGCGAGTGCATGTTCGGCGGTCCTTTCGGCGCGTTCAGAACCCGGGGCGTCCAATTT
TTTGACGAGGAAAACCCGGTCTACCGCGAGTTCGCGAAGATTCTCGCCCTGCGCCGGGAGAAGCTCCCCCTGCGGCGCGG
GCGCCAGTATCTGCGGGAGATCTCGGGGGACGGGGTCACTTTCGGGCTTCCCTATGTCATCGGGGGCGAGATTCGCTCGG
TGGTCCCCTGGTCCCGCATCTTTGACACCGAAGAGATGGTTCTTGCCATCAACACCGATGCCGACTCCCCCCGCACCGCC
TGGGTGCTGGTTGACGGGAGCCTCCACCGGGAGGGTGACCGCTTCGCCTACCGCTACTCCACCGATCCGGGGCAGGTACG
CGGAACAACCGCTGTCCAGCGGATCAACGATGCCGTTCAGGCGATACAGGTGACGGTTCCCGCAGCCGGTTTCGTGATGC
TTGAACAGCGATGA

Upstream 100 bases:

>100_bases
ACAAGCACTTCGGGAAGATCGTGCTGAAGATATCGTAGGGTACACGACACAGGAAGGGGCGCGGCGAACGGCGCCCCTTT
CCTCTTGGGGAGATGACGCC

Downstream 100 bases:

>100_bases
CATCTGCTGATGTCGTGGCGGCGAAGGAGGTGTCGTCATGGCCCATACTTTGCTGTGTGAACCAAGTTCTACATTTCAAT
TCAGGGAGAGCTGCATGTGG

Product: Alpha amylase, catalytic region

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 617; Mature: 616

Protein sequence:

>617_residues
MTVPQSITELDLKNLTNRTFHPSPVAWEDQVLYFLLLDRFSDGKETGYRDNRGRRVAKGETPLYQPGDAGNAIRTEPEAA
VWRESGSRWCGGTLKGLASKMGYLKRMGVTAVWVSPLFKQCSFVPTYHGYGIQNFLEVDPHFGTADDLRELVHVAHANGI
YVILDIILNHAGNIFAYDRGYTPYDGSTVYPVTGFRSADGTPNLPFGPIDTKANPDAWPDGAVWPRELQAANAYTRMGHI
RDWDSYHEYLDGDFFDLKDIHLGEGEVDAFRPSPALLALCEVYKYWIALSDVDGYRIDTVKHMGAGATRFFASVIHEFAQ
AIGKENFYLIGEITGGRDNAFNTLATTGLNAALGVDDIPLRLEDLVKGRANPADYFNLFRNSLLVRKESHVWFRDKVVTV
IDDHDQVCRGAKKERFCAGDPKWRRLVLNALALNTTTLGIPCVYYGTEQAFDGEGGDDRYLRECMFGGPFGAFRTRGVQF
FDEENPVYREFAKILALRREKLPLRRGRQYLREISGDGVTFGLPYVIGGEIRSVVPWSRIFDTEEMVLAINTDADSPRTA
WVLVDGSLHREGDRFAYRYSTDPGQVRGTTAVQRINDAVQAIQVTVPAAGFVMLEQR

Sequences:

>Translated_617_residues
MTVPQSITELDLKNLTNRTFHPSPVAWEDQVLYFLLLDRFSDGKETGYRDNRGRRVAKGETPLYQPGDAGNAIRTEPEAA
VWRESGSRWCGGTLKGLASKMGYLKRMGVTAVWVSPLFKQCSFVPTYHGYGIQNFLEVDPHFGTADDLRELVHVAHANGI
YVILDIILNHAGNIFAYDRGYTPYDGSTVYPVTGFRSADGTPNLPFGPIDTKANPDAWPDGAVWPRELQAANAYTRMGHI
RDWDSYHEYLDGDFFDLKDIHLGEGEVDAFRPSPALLALCEVYKYWIALSDVDGYRIDTVKHMGAGATRFFASVIHEFAQ
AIGKENFYLIGEITGGRDNAFNTLATTGLNAALGVDDIPLRLEDLVKGRANPADYFNLFRNSLLVRKESHVWFRDKVVTV
IDDHDQVCRGAKKERFCAGDPKWRRLVLNALALNTTTLGIPCVYYGTEQAFDGEGGDDRYLRECMFGGPFGAFRTRGVQF
FDEENPVYREFAKILALRREKLPLRRGRQYLREISGDGVTFGLPYVIGGEIRSVVPWSRIFDTEEMVLAINTDADSPRTA
WVLVDGSLHREGDRFAYRYSTDPGQVRGTTAVQRINDAVQAIQVTVPAAGFVMLEQR
>Mature_616_residues
TVPQSITELDLKNLTNRTFHPSPVAWEDQVLYFLLLDRFSDGKETGYRDNRGRRVAKGETPLYQPGDAGNAIRTEPEAAV
WRESGSRWCGGTLKGLASKMGYLKRMGVTAVWVSPLFKQCSFVPTYHGYGIQNFLEVDPHFGTADDLRELVHVAHANGIY
VILDIILNHAGNIFAYDRGYTPYDGSTVYPVTGFRSADGTPNLPFGPIDTKANPDAWPDGAVWPRELQAANAYTRMGHIR
DWDSYHEYLDGDFFDLKDIHLGEGEVDAFRPSPALLALCEVYKYWIALSDVDGYRIDTVKHMGAGATRFFASVIHEFAQA
IGKENFYLIGEITGGRDNAFNTLATTGLNAALGVDDIPLRLEDLVKGRANPADYFNLFRNSLLVRKESHVWFRDKVVTVI
DDHDQVCRGAKKERFCAGDPKWRRLVLNALALNTTTLGIPCVYYGTEQAFDGEGGDDRYLRECMFGGPFGAFRTRGVQFF
DEENPVYREFAKILALRREKLPLRRGRQYLREISGDGVTFGLPYVIGGEIRSVVPWSRIFDTEEMVLAINTDADSPRTAW
VLVDGSLHREGDRFAYRYSTDPGQVRGTTAVQRINDAVQAIQVTVPAAGFVMLEQR

Specific function: May Play A Role In Regulating The Intracellular Level Of Maltotriose. Cleaves Glucose From The Reducing End Of Maltotriose And Longer Maltodextrins With A Chain Length Of Up To 7 Glucose Units. [C]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1786604, Length=503, Percent_Identity=23.8568588469185, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789995, Length=196, Percent_Identity=27.5510204081633, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1790687, Length=265, Percent_Identity=24.9056603773585, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322245, Length=413, Percent_Identity=23.0024213075061, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24586587, Length=214, Percent_Identity=29.4392523364486, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24586599, Length=82, Percent_Identity=40.2439024390244, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24586597, Length=82, Percent_Identity=40.2439024390244, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24586593, Length=82, Percent_Identity=42.6829268292683, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI221330053, Length=82, Percent_Identity=40.2439024390244, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24586591, Length=82, Percent_Identity=41.4634146341463, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24583749, Length=82, Percent_Identity=39.0243902439024, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24583747, Length=82, Percent_Identity=40.2439024390244, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24583745, Length=82, Percent_Identity=36.5853658536585, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR005323 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF03714 PUD [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 69226; Mature: 69095

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVPQSITELDLKNLTNRTFHPSPVAWEDQVLYFLLLDRFSDGKETGYRDNRGRRVAKGE
CCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCC
TPLYQPGDAGNAIRTEPEAAVWRESGSRWCGGTLKGLASKMGYLKRMGVTAVWVSPLFKQ
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
CSFVPTYHGYGIQNFLEVDPHFGTADDLRELVHVAHANGIYVILDIILNHAGNIFAYDRG
CCCCCCCCCCCCCHHEECCCCCCCHHHHHHHHHHHCCCCCEEHHHHHHHCCCCEEEECCC
YTPYDGSTVYPVTGFRSADGTPNLPFGPIDTKANPDAWPDGAVWPRELQAANAYTRMGHI
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
RDWDSYHEYLDGDFFDLKDIHLGEGEVDAFRPSPALLALCEVYKYWIALSDVDGYRIDTV
CCHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCCCEEEHH
KHMGAGATRFFASVIHEFAQAIGKENFYLIGEITGGRDNAFNTLATTGLNAALGVDDIPL
HHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHCCCCCCCCCCCCCC
RLEDLVKGRANPADYFNLFRNSLLVRKESHVWFRDKVVTVIDDHDQVCRGAKKERFCAGD
HHHHHHCCCCCHHHHHHHHHHHHEEEECCCEEEECEEEEEECCCHHHHCCCCCCCCCCCC
PKWRRLVLNALALNTTTLGIPCVYYGTEQAFDGEGGDDRYLRECMFGGPFGAFRTRGVQF
HHHHHHHHHHHHHCCEEECCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCHHHHHCCCEE
FDEENPVYREFAKILALRREKLPLRRGRQYLREISGDGVTFGLPYVIGGEIRSVVPWSRI
ECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEECCCEEECCCHHHHCCHHHH
FDTEEMVLAINTDADSPRTAWVLVDGSLHREGDRFAYRYSTDPGQVRGTTAVQRINDAVQ
CCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHH
AIQVTVPAAGFVMLEQR
HEEEEECCCCEEEEECC
>Mature Secondary Structure 
TVPQSITELDLKNLTNRTFHPSPVAWEDQVLYFLLLDRFSDGKETGYRDNRGRRVAKGE
CCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCC
TPLYQPGDAGNAIRTEPEAAVWRESGSRWCGGTLKGLASKMGYLKRMGVTAVWVSPLFKQ
CCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
CSFVPTYHGYGIQNFLEVDPHFGTADDLRELVHVAHANGIYVILDIILNHAGNIFAYDRG
CCCCCCCCCCCCCHHEECCCCCCCHHHHHHHHHHHCCCCCEEHHHHHHHCCCCEEEECCC
YTPYDGSTVYPVTGFRSADGTPNLPFGPIDTKANPDAWPDGAVWPRELQAANAYTRMGHI
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
RDWDSYHEYLDGDFFDLKDIHLGEGEVDAFRPSPALLALCEVYKYWIALSDVDGYRIDTV
CCHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHEEECCCCCEEEHH
KHMGAGATRFFASVIHEFAQAIGKENFYLIGEITGGRDNAFNTLATTGLNAALGVDDIPL
HHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHCCCCCCCCCCCCCC
RLEDLVKGRANPADYFNLFRNSLLVRKESHVWFRDKVVTVIDDHDQVCRGAKKERFCAGD
HHHHHHCCCCCHHHHHHHHHHHHEEEECCCEEEECEEEEEECCCHHHHCCCCCCCCCCCC
PKWRRLVLNALALNTTTLGIPCVYYGTEQAFDGEGGDDRYLRECMFGGPFGAFRTRGVQF
HHHHHHHHHHHHHCCEEECCEEEEECCCCCCCCCCCCHHHHHHHHCCCCCHHHHHCCCEE
FDEENPVYREFAKILALRREKLPLRRGRQYLREISGDGVTFGLPYVIGGEIRSVVPWSRI
ECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEECCCEEECCCHHHHCCHHHH
FDTEEMVLAINTDADSPRTAWVLVDGSLHREGDRFAYRYSTDPGQVRGTTAVQRINDAVQ
CCCCCEEEEEECCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHH
AIQVTVPAAGFVMLEQR
HEEEEECCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8424949; 8485150 [H]