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The map label for this gene is yogA [H]

Identifier: 78222052

GI number: 78222052

Start: 917464

End: 918450

Strand: Direct

Name: yogA [H]

Synonym: Gmet_0832

Alternate gene names: 78222052

Gene position: 917464-918450 (Clockwise)

Preceding gene: 78222051

Following gene: 78222053

Centisome position: 22.95

GC content: 61.6

Gene sequence:

>987_bases
ATGAAAGCAGTTCTGCTCAATGGTTTCGGCGGTCTCGATGTGCTCACGGTGGGGGAGGTTGAGAGGCCGAAACCGGGGGA
GGGGCAGGTTCTTGTGAAAGTGGTGGCAACGTCCATCAATCGTCCGGACCTGGTGCAGCGGGAAGGGAAGTATCCCCCTC
CGCCGGGCGATTCTGAGATCCTGGGGTTGGAGGTTTCCGGCACCGTTGAAGAGTTGGGCCCCGGCGTGACCGGCTGGCAG
GTGGGGGACCGGGTCATGACGCTGGTGGGTGGCGGCGCCTACGCCGAGTATGCCGTGGCCTACGCCAGTCACCTGATGCG
GATCCCCGAGTCCATGAGCTTCGAGGAGGCGGCCTGCGTCTGCGAATCGTGCATCACGGCGTTTCTCAACGTCTTCATGA
TCGGCGGCCTCAAGGACAACAACACCGTCATCCTCCACGGCGGCGGCGGGGGGGTAAATACCGCCGGCCTTCAACTCTGC
AAGGCCCTGGTTCCCAATACGAAGATCATCGTCACCTCCCATCCGAGCAAGATGGCGCGGGTGAAGGAACTGGGCGCCGA
CCTGGTCATCGACTTCACCCAGACCCCCGATTTCTCGGAGGCGGTGAAGGAGTTCACCAACAAGAAGGGGGCTGACGTGA
TCCTCGACCATGTGGGGGCCAAGTACCTGGCGCCCAACATGAACTCCCTGGCCTATGCCGGCCGCCTCGTCATCATCGGG
GTCATCAGCGGCATCAAGGCGGAGTTGAACCTGGCCCTCATGATGGTGAAGCGGCAGCAGATCATCGGTTCCGTGCTCCG
CTCCCGCCCCGTGAAGGACAAGGGGGAGATCGTTGCCGAGTTCACCAGAACGGCGCTGCCGAAATTCGCCGACCGGACCA
TTGTCCCCATCATCGAGAAGGTGTTCACCATCGACCAGGTGGTGGAAGCCCACCGGATGATGGAAGAAGACAAGCACTTC
GGGAAGATCGTGCTGAAGATATCGTAG

Upstream 100 bases:

>100_bases
TATGACCGCTCTCCGGATTTCCTGTTTACACAATCCCGCCACTTCGCTAGAGTTGCCGGATTGATTTTGTCAGCTATCCA
ATGCAAGGAGGAAAAGGCGT

Downstream 100 bases:

>100_bases
GGTACACGACACAGGAAGGGGCGCGGCGAACGGCGCCCCTTTCCTCTTGGGGAGATGACGCCATGACGGTACCGCAATCG
ATCACGGAACTGGATCTCAA

Product: alcohol dehydrogenase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKAVLLNGFGGLDVLTVGEVERPKPGEGQVLVKVVATSINRPDLVQREGKYPPPPGDSEILGLEVSGTVEELGPGVTGWQ
VGDRVMTLVGGGAYAEYAVAYASHLMRIPESMSFEEAACVCESCITAFLNVFMIGGLKDNNTVILHGGGGGVNTAGLQLC
KALVPNTKIIVTSHPSKMARVKELGADLVIDFTQTPDFSEAVKEFTNKKGADVILDHVGAKYLAPNMNSLAYAGRLVIIG
VISGIKAELNLALMMVKRQQIIGSVLRSRPVKDKGEIVAEFTRTALPKFADRTIVPIIEKVFTIDQVVEAHRMMEEDKHF
GKIVLKIS

Sequences:

>Translated_328_residues
MKAVLLNGFGGLDVLTVGEVERPKPGEGQVLVKVVATSINRPDLVQREGKYPPPPGDSEILGLEVSGTVEELGPGVTGWQ
VGDRVMTLVGGGAYAEYAVAYASHLMRIPESMSFEEAACVCESCITAFLNVFMIGGLKDNNTVILHGGGGGVNTAGLQLC
KALVPNTKIIVTSHPSKMARVKELGADLVIDFTQTPDFSEAVKEFTNKKGADVILDHVGAKYLAPNMNSLAYAGRLVIIG
VISGIKAELNLALMMVKRQQIIGSVLRSRPVKDKGEIVAEFTRTALPKFADRTIVPIIEKVFTIDQVVEAHRMMEEDKHF
GKIVLKIS
>Mature_328_residues
MKAVLLNGFGGLDVLTVGEVERPKPGEGQVLVKVVATSINRPDLVQREGKYPPPPGDSEILGLEVSGTVEELGPGVTGWQ
VGDRVMTLVGGGAYAEYAVAYASHLMRIPESMSFEEAACVCESCITAFLNVFMIGGLKDNNTVILHGGGGGVNTAGLQLC
KALVPNTKIIVTSHPSKMARVKELGADLVIDFTQTPDFSEAVKEFTNKKGADVILDHVGAKYLAPNMNSLAYAGRLVIIG
VISGIKAELNLALMMVKRQQIIGSVLRSRPVKDKGEIVAEFTRTALPKFADRTIVPIIEKVFTIDQVVEAHRMMEEDKHF
GKIVLKIS

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI22538446, Length=334, Percent_Identity=39.2215568862275, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI22538444, Length=334, Percent_Identity=39.2215568862275, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI24308257, Length=347, Percent_Identity=28.2420749279539, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI18379349, Length=342, Percent_Identity=30.9941520467836, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI194239674, Length=333, Percent_Identity=28.8288288288288, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI13236495, Length=333, Percent_Identity=28.8288288288288, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI194239676, Length=332, Percent_Identity=27.1084337349398, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI28557745, Length=233, Percent_Identity=29.1845493562232, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI47519420, Length=342, Percent_Identity=27.7777777777778, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI41872631, Length=309, Percent_Identity=25.5663430420712, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1790485, Length=319, Percent_Identity=29.4670846394984, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1787863, Length=371, Percent_Identity=27.2237196765499, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082125, Length=370, Percent_Identity=26.4864864864865, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17507255, Length=329, Percent_Identity=27.355623100304, Blast_Score=101, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI71987554, Length=355, Percent_Identity=25.0704225352113, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17536829, Length=224, Percent_Identity=32.1428571428571, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562584, Length=357, Percent_Identity=24.6498599439776, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6319520, Length=249, Percent_Identity=30.5220883534137, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323961, Length=339, Percent_Identity=24.4837758112094, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19920632, Length=298, Percent_Identity=24.496644295302, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI221330659, Length=298, Percent_Identity=24.496644295302, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45550423, Length=173, Percent_Identity=31.7919075144509, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24581345, Length=328, Percent_Identity=22.5609756097561, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24581341, Length=366, Percent_Identity=24.3169398907104, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24581339, Length=366, Percent_Identity=24.3169398907104, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 35211; Mature: 35211

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVLLNGFGGLDVLTVGEVERPKPGEGQVLVKVVATSINRPDLVQREGKYPPPPGDSEI
CCEEEEECCCCCEEEEECCCCCCCCCCCCEEEEEEHHCCCCCCHHHHCCCCCCCCCCCCE
LGLEVSGTVEELGPGVTGWQVGDRVMTLVGGGAYAEYAVAYASHLMRIPESMSFEEAACV
EEEEECCCHHHHCCCCCCHHHHHHEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHH
CESCITAFLNVFMIGGLKDNNTVILHGGGGGVNTAGLQLCKALVPNTKIIVTSHPSKMAR
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHH
VKELGADLVIDFTQTPDFSEAVKEFTNKKGADVILDHVGAKYLAPNMNSLAYAGRLVIIG
HHHCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHCCCHHHCCCCCHHHHHCCEEEEE
VISGIKAELNLALMMVKRQQIIGSVLRSRPVKDKGEIVAEFTRTALPKFADRTIVPIIEK
EHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
VFTIDQVVEAHRMMEEDKHFGKIVLKIS
HHHHHHHHHHHHHHHHCCCCCEEEEEEC
>Mature Secondary Structure
MKAVLLNGFGGLDVLTVGEVERPKPGEGQVLVKVVATSINRPDLVQREGKYPPPPGDSEI
CCEEEEECCCCCEEEEECCCCCCCCCCCCEEEEEEHHCCCCCCHHHHCCCCCCCCCCCCE
LGLEVSGTVEELGPGVTGWQVGDRVMTLVGGGAYAEYAVAYASHLMRIPESMSFEEAACV
EEEEECCCHHHHCCCCCCHHHHHHEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHH
CESCITAFLNVFMIGGLKDNNTVILHGGGGGVNTAGLQLCKALVPNTKIIVTSHPSKMAR
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCHHHHH
VKELGADLVIDFTQTPDFSEAVKEFTNKKGADVILDHVGAKYLAPNMNSLAYAGRLVIIG
HHHCCCCEEEEECCCCCHHHHHHHHHCCCCCHHHHHHCCCHHHCCCCCHHHHHCCEEEEE
VISGIKAELNLALMMVKRQQIIGSVLRSRPVKDKGEIVAEFTRTALPKFADRTIVPIIEK
EHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
VFTIDQVVEAHRMMEEDKHFGKIVLKIS
HHHHHHHHHHHHHHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]