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The map label for this gene is rffG [C]

Identifier: 78222043

GI number: 78222043

Start: 908533

End: 909432

Strand: Direct

Name: rffG [C]

Synonym: Gmet_0823

Alternate gene names: 78222043

Gene position: 908533-909432 (Clockwise)

Preceding gene: 78222042

Following gene: 78222045

Centisome position: 22.73

GC content: 62.78

Gene sequence:

>900_bases
GTGACACCGGGGATGGGAGAAGCAAACGTGGCGCAGGTGTTCATCGTCGGGTGCGGCGATATCGGAAAACGGGTGGCACG
GCTTGCCATGGATAAGGGAGTTGCGGTGACAGCCCTTGTCCGTTCGGAGGAAAGCGCCGCCAAACTGCATGAACTTGGCA
TCGAAACGGTGGAAGGGCACCTTGACGACCCCGAATCCCTTGCGGGCCTCCCCCTCCGGGGGGCGACCGTGTTCTACTTT
GCCCCTCCGCCGGGTGGAGGGATAACCGAGCCGAGGGTTCGGGCCTTCTGCGCAGCCGTCAGGCCGGGCGACGAGCCGGC
ACAGGTGGTTTACCTCAGCACCAGCGGCGTCTATGGCGACTGCGGCGACATGATCGTGACCGAAGATACGCCTGCCAATC
CCCAGACGGCACGGGCCAAGCGCCGCTACGACGCCGAAACGGTCTTTCGCGCCTGGGGCAAGGAGCGAGGCGTTCCGATC
GTCGTGCTTCGGGTCACGGGCATCTACGGTCCGGGGCGGCTTCCGCTCCAGCAGCTGACGAGCGGCCAGCCGGTTCTCTT
CGAGAGTGAGGCCTCCTATACCAATCGGATCCATTCCGAGGACCTGGCCCGGGTCTGCATGGCTGCTGCCGAGAAGGGCG
AGGACGGCGACATATTCAATGTGAGTGACGGCAACCCGGGCACCATGACCGAGTACTTCAACGCCTGCGCCGATGCCCTC
GGTTTTCCGCGCCCCCGGCAGGTGACCATGGAGGAGGCGAAGAAAGTCATGACACCCCTCATGCTTTCCTATGTTACGGA
GTCGCGGCGGATGGACAACGCGAAAATGGTGGGTAAACTAGAGGTTAAACTTCTGTACCCGACCCTCCAGGATGGTCTGA
AGGCAAGCGTTGCCAAATAG

Upstream 100 bases:

>100_bases
TGGGGAGAACAGCCAGCCGTGAGTAGTATTGACATGATTCCTCCTCAGGTGGTATTAGAGCTGATTCGCTAATGAGCGGC
GGTTATGGGGTGCCGGTGTC

Downstream 100 bases:

>100_bases
TCGGGGAGTGCGGCAGCAAAGGGGTTATTGCAGATAGCGTTCCGATGACATCACCTTGGAGATGAGGAGTAACTGCCTGA
GGTGGGGCGAACTGTTGCCC

Product: 3-beta hydroxysteroid dehydrogenase/isomerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: Nucleoside-Diphosphate-Sugar Epimerase; Oxidoreductase Protein; Nucleoside-Diphosphate-Sugar Epimerases; 3-Beta Hydroxysteroid Dehydrogenase/Isomerase; Nucleoside-Diphosphate-Sugar Epimerase Protein; NAD Dependent Epimerase/Dehydratase; NAD Dependent Epimerase/Dehydratase Family; NAD-Dependent Epimerase/Dehydratase Family Protein; NAD Dependent Epimerase/Dehydratase Family Protein; ActC Family Protein; Sugar Epimerase/Dehydratase-Like Protein; Epimerase; Actc Protein; 3-Beta Hydroxysteroid Dehydrogenase/Isomerase Family; Oxidoreductase; NAD Epimerase/Dehydratase Protein; NAD-Dependent Epimerase

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTPGMGEANVAQVFIVGCGDIGKRVARLAMDKGVAVTALVRSEESAAKLHELGIETVEGHLDDPESLAGLPLRGATVFYF
APPPGGGITEPRVRAFCAAVRPGDEPAQVVYLSTSGVYGDCGDMIVTEDTPANPQTARAKRRYDAETVFRAWGKERGVPI
VVLRVTGIYGPGRLPLQQLTSGQPVLFESEASYTNRIHSEDLARVCMAAAEKGEDGDIFNVSDGNPGTMTEYFNACADAL
GFPRPRQVTMEEAKKVMTPLMLSYVTESRRMDNAKMVGKLEVKLLYPTLQDGLKASVAK

Sequences:

>Translated_299_residues
MTPGMGEANVAQVFIVGCGDIGKRVARLAMDKGVAVTALVRSEESAAKLHELGIETVEGHLDDPESLAGLPLRGATVFYF
APPPGGGITEPRVRAFCAAVRPGDEPAQVVYLSTSGVYGDCGDMIVTEDTPANPQTARAKRRYDAETVFRAWGKERGVPI
VVLRVTGIYGPGRLPLQQLTSGQPVLFESEASYTNRIHSEDLARVCMAAAEKGEDGDIFNVSDGNPGTMTEYFNACADAL
GFPRPRQVTMEEAKKVMTPLMLSYVTESRRMDNAKMVGKLEVKLLYPTLQDGLKASVAK
>Mature_298_residues
TPGMGEANVAQVFIVGCGDIGKRVARLAMDKGVAVTALVRSEESAAKLHELGIETVEGHLDDPESLAGLPLRGATVFYFA
PPPGGGITEPRVRAFCAAVRPGDEPAQVVYLSTSGVYGDCGDMIVTEDTPANPQTARAKRRYDAETVFRAWGKERGVPIV
VLRVTGIYGPGRLPLQQLTSGQPVLFESEASYTNRIHSEDLARVCMAAAEKGEDGDIFNVSDGNPGTMTEYFNACADALG
FPRPRQVTMEEAKKVMTPLMLSYVTESRRMDNAKMVGKLEVKLLYPTLQDGLKASVAK

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 32141; Mature: 32010

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPGMGEANVAQVFIVGCGDIGKRVARLAMDKGVAVTALVRSEESAAKLHELGIETVEGH
CCCCCCCCCEEEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCHHHHHCC
LDDPESLAGLPLRGATVFYFAPPPGGGITEPRVRAFCAAVRPGDEPAQVVYLSTSGVYGD
CCCHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCC
CGDMIVTEDTPANPQTARAKRRYDAETVFRAWGKERGVPIVVLRVTGIYGPGRLPLQQLT
CCCEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHC
SGQPVLFESEASYTNRIHSEDLARVCMAAAEKGEDGDIFNVSDGNPGTMTEYFNACADAL
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHH
GFPRPRQVTMEEAKKVMTPLMLSYVTESRRMDNAKMVGKLEVKLLYPTLQDGLKASVAK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCHHHHHHHHHCCC
>Mature Secondary Structure 
TPGMGEANVAQVFIVGCGDIGKRVARLAMDKGVAVTALVRSEESAAKLHELGIETVEGH
CCCCCCCCEEEEEEEECCHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHCHHHHHCC
LDDPESLAGLPLRGATVFYFAPPPGGGITEPRVRAFCAAVRPGDEPAQVVYLSTSGVYGD
CCCHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCC
CGDMIVTEDTPANPQTARAKRRYDAETVFRAWGKERGVPIVVLRVTGIYGPGRLPLQQLT
CCCEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHHC
SGQPVLFESEASYTNRIHSEDLARVCMAAAEKGEDGDIFNVSDGNPGTMTEYFNACADAL
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHH
GFPRPRQVTMEEAKKVMTPLMLSYVTESRRMDNAKMVGKLEVKLLYPTLQDGLKASVAK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA