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The map label for this gene is mdeA [H]

Identifier: 78222039

GI number: 78222039

Start: 904271

End: 905554

Strand: Reverse

Name: mdeA [H]

Synonym: Gmet_0819

Alternate gene names: 78222039

Gene position: 905554-904271 (Counterclockwise)

Preceding gene: 78222040

Following gene: 78222035

Centisome position: 22.65

GC content: 65.5

Gene sequence:

>1284_bases
ATGAGCAGTTCGTGGAAGATCGAGACCCAGGCCATCCAGGGGGGATACACCCCCAAGGCGGGCGACCCCCGCGTCGTCAC
CATCTGCCAGAGCACCACCTTCAAGTACGACAATGCCGACCACGTGACGAAGCTCTTCGACCTGGAAGAGCCCGGCTTTT
TCTATACCCGCCTGGGCAACCCCACCACCGACGCCTTCGAGCAGAAGCTGGCCATGATGGAGGGGGGAGTCGGGGCCCTG
GCCACCTCCGCCGGCCAGGCGGCCACAACCCTTTCCATCTTCAACATTTGCCAGGCGGGCCAGCACTTCGTGACCGCCAG
CACCCTCTATGGCGGCACCTACAACCTCTTCGCCTGCACCCTCCCCAAGATGGGGATCGAAGTGTCCTTCGTGGACCCGG
AGGCCCCGGCCGACGAGATCCTCGCCGCCTTCCGCCCCAACACCAAGGCCCTCTTCGCCGAGACCATCGGCAACCCCGGC
CTGAACGTCCTGGACTTCGCCAAATTCAGCGCCATCGCCCAGGAGAAGGGGGTGCCACTCATCATCGACAGCACCTTCGC
CACTCCCTACCTCTGCCGCCCCTTCGAGCACGGGGCCAACGTGGTGGTCCACTCGGCCACGAAATACATCGACGGTCTCG
CCACCAGCGTCGGCGGCGTCATCGTGGACGGGGGGAACTTCGACTGGGGGAACGGCCGCTTCCCTGAGCTGACCGAGCCC
GACTCCTCCTACCATGGCCTCCAGTACGTGAAGACCTTCGGGAACCTGGCTTACATCATCAAGGCCCGGGTCCAGCTCAT
GCGCGACATCGGCACCACGCCGAGCCCCTTCAACTCATTCCTCTTCACCAACGGCCTCCAGACCCTGCCGCTCCGGATGC
AGCGCCACAGCGACAACGCCCTGGCCCTGGCCCAGTTCCTGGAAAGCCACCCATCGGTCAGTTGGGTCAAGTACCCCGGT
CTCTCAAGCCACGCCAGCCACGACCGGGCGCAAAAGTACCTGCCGCAAGGATGCAGCGGCGTCCTCACCTTCGGCATCAA
GGGGGGCACCGAGGCCGGCAAGAAGTTCATGGAGGCGTGCCGGCTCGTGGCCCTCGTGGTCCACGTGGGGGACGCCCGCA
GCTGCGTCCTTCACCCGGCCAGTACCACCCACCGCCAGCTAACCGCCGAGCAGCAGACCGCCTCCGGTGTCACGCCGGAC
CTGATCCGCCTCTCCGTCGGCATCGAGCACATCGACGACATCATCGCCGACGTGGACCAGGCCCTGGCTGCAAGCCAGAA
GTAG

Upstream 100 bases:

>100_bases
CCAGGTGGCGCGGGAGATGGAGCTGCATTAAATCCGGGTTTCCCGTACGGAAATTCTTTGGTATCATCGCGGTCCCATTA
CTCGTTGAAAGGAGACCGCC

Downstream 100 bases:

>100_bases
CCGACTATTTTCCAATCCAGACGCAGAAAAGCCTGCCAGTTATCCGGCAGGCTTTTTCTATTGATGCAAGCAACTGGAGA
TCAGTATTCCGTGGGCTTCT

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MSSSWKIETQAIQGGYTPKAGDPRVVTICQSTTFKYDNADHVTKLFDLEEPGFFYTRLGNPTTDAFEQKLAMMEGGVGAL
ATSAGQAATTLSIFNICQAGQHFVTASTLYGGTYNLFACTLPKMGIEVSFVDPEAPADEILAAFRPNTKALFAETIGNPG
LNVLDFAKFSAIAQEKGVPLIIDSTFATPYLCRPFEHGANVVVHSATKYIDGLATSVGGVIVDGGNFDWGNGRFPELTEP
DSSYHGLQYVKTFGNLAYIIKARVQLMRDIGTTPSPFNSFLFTNGLQTLPLRMQRHSDNALALAQFLESHPSVSWVKYPG
LSSHASHDRAQKYLPQGCSGVLTFGIKGGTEAGKKFMEACRLVALVVHVGDARSCVLHPASTTHRQLTAEQQTASGVTPD
LIRLSVGIEHIDDIIADVDQALAASQK

Sequences:

>Translated_427_residues
MSSSWKIETQAIQGGYTPKAGDPRVVTICQSTTFKYDNADHVTKLFDLEEPGFFYTRLGNPTTDAFEQKLAMMEGGVGAL
ATSAGQAATTLSIFNICQAGQHFVTASTLYGGTYNLFACTLPKMGIEVSFVDPEAPADEILAAFRPNTKALFAETIGNPG
LNVLDFAKFSAIAQEKGVPLIIDSTFATPYLCRPFEHGANVVVHSATKYIDGLATSVGGVIVDGGNFDWGNGRFPELTEP
DSSYHGLQYVKTFGNLAYIIKARVQLMRDIGTTPSPFNSFLFTNGLQTLPLRMQRHSDNALALAQFLESHPSVSWVKYPG
LSSHASHDRAQKYLPQGCSGVLTFGIKGGTEAGKKFMEACRLVALVVHVGDARSCVLHPASTTHRQLTAEQQTASGVTPD
LIRLSVGIEHIDDIIADVDQALAASQK
>Mature_426_residues
SSSWKIETQAIQGGYTPKAGDPRVVTICQSTTFKYDNADHVTKLFDLEEPGFFYTRLGNPTTDAFEQKLAMMEGGVGALA
TSAGQAATTLSIFNICQAGQHFVTASTLYGGTYNLFACTLPKMGIEVSFVDPEAPADEILAAFRPNTKALFAETIGNPGL
NVLDFAKFSAIAQEKGVPLIIDSTFATPYLCRPFEHGANVVVHSATKYIDGLATSVGGVIVDGGNFDWGNGRFPELTEPD
SSYHGLQYVKTFGNLAYIIKARVQLMRDIGTTPSPFNSFLFTNGLQTLPLRMQRHSDNALALAQFLESHPSVSWVKYPGL
SSHASHDRAQKYLPQGCSGVLTFGIKGGTEAGKKFMEACRLVALVVHVGDARSCVLHPASTTHRQLTAEQQTASGVTPDL
IRLSVGIEHIDDIIADVDQALAASQK

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=421, Percent_Identity=33.4916864608076, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI299473759, Length=421, Percent_Identity=30.8788598574822, Blast_Score=168, Evalue=1e-41,
Organism=Homo sapiens, GI25453487, Length=420, Percent_Identity=30.2380952380952, Blast_Score=158, Evalue=1e-38,
Organism=Escherichia coli, GI1790375, Length=373, Percent_Identity=30.5630026809651, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI1789383, Length=427, Percent_Identity=26.2295081967213, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI71980708, Length=405, Percent_Identity=32.8395061728395, Blast_Score=228, Evalue=4e-60,
Organism=Caenorhabditis elegans, GI17538055, Length=420, Percent_Identity=35.7142857142857, Blast_Score=212, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17560056, Length=419, Percent_Identity=34.6062052505967, Blast_Score=203, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6323333, Length=412, Percent_Identity=46.8446601941748, Blast_Score=362, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6319307, Length=428, Percent_Identity=33.1775700934579, Blast_Score=202, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6321170, Length=328, Percent_Identity=27.1341463414634, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321254, Length=445, Percent_Identity=24.0449438202247, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19922554, Length=427, Percent_Identity=33.0210772833724, Blast_Score=208, Evalue=7e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 45948; Mature: 45817

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSWKIETQAIQGGYTPKAGDPRVVTICQSTTFKYDNADHVTKLFDLEEPGFFYTRLGN
CCCCCEEEEEEECCCCCCCCCCCCEEEEECCCEECCCCHHHHHHHHCCCCCCEEEEECCC
PTTDAFEQKLAMMEGGVGALATSAGQAATTLSIFNICQAGQHFVTASTLYGGTYNLFACT
CCHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEE
LPKMGIEVSFVDPEAPADEILAAFRPNTKALFAETIGNPGLNVLDFAKFSAIAQEKGVPL
CCCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCE
IIDSTFATPYLCRPFEHGANVVVHSATKYIDGLATSVGGVIVDGGNFDWGNGRFPELTEP
EEECCCCCCCEECCHHCCCCEEEECHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCC
DSSYHGLQYVKTFGNLAYIIKARVQLMRDIGTTPSPFNSFLFTNGLQTLPLRMQRHSDNA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHCCHHHHHCCCCH
LALAQFLESHPSVSWVKYPGLSSHASHDRAQKYLPQGCSGVLTFGIKGGTEAGKKFMEAC
HHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHH
RLVALVVHVGDARSCVLHPASTTHRQLTAEQQTASGVTPDLIRLSVGIEHIDDIIADVDQ
HHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHH
ALAASQK
HHHCCCC
>Mature Secondary Structure 
SSSWKIETQAIQGGYTPKAGDPRVVTICQSTTFKYDNADHVTKLFDLEEPGFFYTRLGN
CCCCEEEEEEECCCCCCCCCCCCEEEEECCCEECCCCHHHHHHHHCCCCCCEEEEECCC
PTTDAFEQKLAMMEGGVGALATSAGQAATTLSIFNICQAGQHFVTASTLYGGTYNLFACT
CCHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEE
LPKMGIEVSFVDPEAPADEILAAFRPNTKALFAETIGNPGLNVLDFAKFSAIAQEKGVPL
CCCCCEEEEEECCCCCHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCE
IIDSTFATPYLCRPFEHGANVVVHSATKYIDGLATSVGGVIVDGGNFDWGNGRFPELTEP
EEECCCCCCCEECCHHCCCCEEEECHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCC
DSSYHGLQYVKTFGNLAYIIKARVQLMRDIGTTPSPFNSFLFTNGLQTLPLRMQRHSDNA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHCCHHHHHCCCCH
LALAQFLESHPSVSWVKYPGLSSHASHDRAQKYLPQGCSGVLTFGIKGGTEAGKKFMEAC
HHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHH
RLVALVVHVGDARSCVLHPASTTHRQLTAEQQTASGVTPDLIRLSVGIEHIDDIIADVDQ
HHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHH
ALAASQK
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]