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The map label for this gene is oprM [H]

Identifier: 78222031

GI number: 78222031

Start: 892515

End: 894068

Strand: Direct

Name: oprM [H]

Synonym: Gmet_0811

Alternate gene names: 78222031

Gene position: 892515-894068 (Clockwise)

Preceding gene: 78222030

Following gene: 78222033

Centisome position: 22.33

GC content: 62.93

Gene sequence:

>1554_bases
ATGACTATGGAAAAATTCGAAATAACACAGAGCAACAGAGCAACAGAGGGGAAATCGGATTTCGCGGCACAATTTTACCT
CAATACCCAACAGCGGTCGCCGAGTCCTAGCGGTTCCACGTCGAAGCCTTTCTCTGTTGCTCAGTTGCTCTGTGTTTCAA
TTATGACTTTGACCCTGGCCGGCTGCACCACCATGGCCCCCAAATACGAGCGTCCCGCCGCACCCGTTTCGGCTGCATGG
CCGGAAGGCCCGGCGTATAAGGAGACTGGCGTCAGGTCCGGAAAGCCGGTGGCCGACATCCCGTGGCAGGAGTTCTTCGA
GGACGAAAAGCTGCGGAAGCTGATTGCCCTGGCCCTGGAGAACAACCGGGACCTGCGGGTGGCCGCCCTCAACATAGAGC
GCTCCCGGGCCCAGTATCGGATTCAACGCTCCGATCTCTTTCCGAAGGTGGACGCCACCGCCGATGGCAGCGTCCAGAGA
ATCCCCCAGGATCTCTCTGCTACCGGCCAGGCCCGCACCGTCGACCAGTACAGCGTCGGTCTTGGGGTCAGTTCCTATGA
GCTGGATCTCTTCGGTCGGGTGCGGAGCCTCAAGGACCAAGCGCTGGAGCAGTACCTGGCCACGGAACAGGCCCGCCGCA
GTGTGCAGATCAGCCTGGTGGCCGAGGTGGCCTCCAACTACCTGACCCTGGCCGCCGACCGGGAACGGCTGAAGCTGGCT
CAGGATACGCTGGCGAGCCAGCAGTCCTCCTACAACCTCACCAAGAGTCGCTTCGAGGCCGGTGTCTCCTCGTTCCTCGA
TCTCAACCAGGCGCGGACCAGCGTGGAGTCGGCCCGGGTGGACATAGCCCGCTACACGACGCTGATAGCCCAAGACGTAA
ATGCCCTCAATCTCGTGGTGGGATCGCCGGTGTCGACGGCGCTCCTCCCCGCTGCCCTCACCGAGAACCTTTCCGCCATG
AAGGATGTGGCGCCGGGGCTGCCGTCCGACGTGCTACTGCGCCGTCCCGACATCCTCCAGGCAGAGAACCTCCTGAAGGG
GGCCAACGCCAACATCGGCGCGGCCCGGGCCAACTTCTTCCCCCGCATTACCCTGCTTTCAAGCGTCGGTTTCGGGAGCG
ACGAGTTGACGGGGCTCTTCTCCGGGAATTCCTTCACCTGGAACTTTGCGCCGCGGATAACACTGCCGATCTTCACCGCC
GGAGCGAACCAGGCCACCCTCAAGGTGGCGGAGGTGGACCGTGATATCGCCGTGGCCCAGTACGAGAAGGCGATCCAGAC
CGCCTTCCGGGAAGTGGCCGACGCCCTGGCCCAGCGGGGAACCATCGACGATCAACTCTCGGCCCAGCAGTCCCTCACCG
ACGCCACCTCCGAAAGCTACCGCCTCTCCCAGGCACGGTACGAAAAAGGAGTCGACAGCTACCTCCAGGTGCTCGATTCC
CAGCGGGCCCTCTACGGAGCCCAGCAGAACCTGATCGGCGTCCGCCTGGTCCGCCTCCTTAACGTGGCGACCCTCTACAA
AGTGTTGGGGGGCGGCGCTCCCGGGCAAGAGTAA

Upstream 100 bases:

>100_bases
TTCATCCCGTTCTTCTTCGTGATGGTGTCGAAGGTGTTTGCCAAGAAAAAGCAGCCTTCCGTTCAGCCGGCCGCTGAAGC
AGCGTCGGAGGTGAATTGAT

Downstream 100 bases:

>100_bases
GTTTCCGCTTCAAAGGTATGGTTAGCCAGGGCCGTGCTGTCGCTTCCGAATCTTCGGGGGCGGCAGCACGGCTTTTCGGT
TTCGGACGGTTTCCAGCGGA

Product: RND efflux system, outer membrane lipoprotein, NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MTMEKFEITQSNRATEGKSDFAAQFYLNTQQRSPSPSGSTSKPFSVAQLLCVSIMTLTLAGCTTMAPKYERPAAPVSAAW
PEGPAYKETGVRSGKPVADIPWQEFFEDEKLRKLIALALENNRDLRVAALNIERSRAQYRIQRSDLFPKVDATADGSVQR
IPQDLSATGQARTVDQYSVGLGVSSYELDLFGRVRSLKDQALEQYLATEQARRSVQISLVAEVASNYLTLAADRERLKLA
QDTLASQQSSYNLTKSRFEAGVSSFLDLNQARTSVESARVDIARYTTLIAQDVNALNLVVGSPVSTALLPAALTENLSAM
KDVAPGLPSDVLLRRPDILQAENLLKGANANIGAARANFFPRITLLSSVGFGSDELTGLFSGNSFTWNFAPRITLPIFTA
GANQATLKVAEVDRDIAVAQYEKAIQTAFREVADALAQRGTIDDQLSAQQSLTDATSESYRLSQARYEKGVDSYLQVLDS
QRALYGAQQNLIGVRLVRLLNVATLYKVLGGGAPGQE

Sequences:

>Translated_517_residues
MTMEKFEITQSNRATEGKSDFAAQFYLNTQQRSPSPSGSTSKPFSVAQLLCVSIMTLTLAGCTTMAPKYERPAAPVSAAW
PEGPAYKETGVRSGKPVADIPWQEFFEDEKLRKLIALALENNRDLRVAALNIERSRAQYRIQRSDLFPKVDATADGSVQR
IPQDLSATGQARTVDQYSVGLGVSSYELDLFGRVRSLKDQALEQYLATEQARRSVQISLVAEVASNYLTLAADRERLKLA
QDTLASQQSSYNLTKSRFEAGVSSFLDLNQARTSVESARVDIARYTTLIAQDVNALNLVVGSPVSTALLPAALTENLSAM
KDVAPGLPSDVLLRRPDILQAENLLKGANANIGAARANFFPRITLLSSVGFGSDELTGLFSGNSFTWNFAPRITLPIFTA
GANQATLKVAEVDRDIAVAQYEKAIQTAFREVADALAQRGTIDDQLSAQQSLTDATSESYRLSQARYEKGVDSYLQVLDS
QRALYGAQQNLIGVRLVRLLNVATLYKVLGGGAPGQE
>Mature_516_residues
TMEKFEITQSNRATEGKSDFAAQFYLNTQQRSPSPSGSTSKPFSVAQLLCVSIMTLTLAGCTTMAPKYERPAAPVSAAWP
EGPAYKETGVRSGKPVADIPWQEFFEDEKLRKLIALALENNRDLRVAALNIERSRAQYRIQRSDLFPKVDATADGSVQRI
PQDLSATGQARTVDQYSVGLGVSSYELDLFGRVRSLKDQALEQYLATEQARRSVQISLVAEVASNYLTLAADRERLKLAQ
DTLASQQSSYNLTKSRFEAGVSSFLDLNQARTSVESARVDIARYTTLIAQDVNALNLVVGSPVSTALLPAALTENLSAMK
DVAPGLPSDVLLRRPDILQAENLLKGANANIGAARANFFPRITLLSSVGFGSDELTGLFSGNSFTWNFAPRITLPIFTAG
ANQATLKVAEVDRDIAVAQYEKAIQTAFREVADALAQRGTIDDQLSAQQSLTDATSESYRLSQARYEKGVDSYLQVLDSQ
RALYGAQQNLIGVRLVRLLNVATLYKVLGGGAPGQE

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=473, Percent_Identity=41.014799154334, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI2367348, Length=490, Percent_Identity=24.4897959183673, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 56241; Mature: 56110

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMEKFEITQSNRATEGKSDFAAQFYLNTQQRSPSPSGSTSKPFSVAQLLCVSIMTLTLA
CCCCCEEECCCCCCCCCCCHHEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
GCTTMAPKYERPAAPVSAAWPEGPAYKETGVRSGKPVADIPWQEFFEDEKLRKLIALALE
HHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
NNRDLRVAALNIERSRAQYRIQRSDLFPKVDATADGSVQRIPQDLSATGQARTVDQYSVG
CCCCEEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHCCCCCCCCCHHHHHC
LGVSSYELDLFGRVRSLKDQALEQYLATEQARRSVQISLVAEVASNYLTLAADRERLKLA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHH
QDTLASQQSSYNLTKSRFEAGVSSFLDLNQARTSVESARVDIARYTTLIAQDVNALNLVV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
GSPVSTALLPAALTENLSAMKDVAPGLPSDVLLRRPDILQAENLLKGANANIGAARANFF
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHCCCCCCCCCHHHCCC
PRITLLSSVGFGSDELTGLFSGNSFTWNFAPRITLPIFTAGANQATLKVAEVDRDIAVAQ
HHHHHHHHCCCCCCCCEEEECCCEEEEECCCEEEEEEEECCCCCCEEEEECCCCHHHHHH
YEKAIQTAFREVADALAQRGTIDDQLSAQQSLTDATSESYRLSQARYEKGVDSYLQVLDS
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRALYGAQQNLIGVRLVRLLNVATLYKVLGGGAPGQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TMEKFEITQSNRATEGKSDFAAQFYLNTQQRSPSPSGSTSKPFSVAQLLCVSIMTLTLA
CCCCEEECCCCCCCCCCCHHEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
GCTTMAPKYERPAAPVSAAWPEGPAYKETGVRSGKPVADIPWQEFFEDEKLRKLIALALE
HHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
NNRDLRVAALNIERSRAQYRIQRSDLFPKVDATADGSVQRIPQDLSATGQARTVDQYSVG
CCCCEEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHCCCCCCCCCHHHHHC
LGVSSYELDLFGRVRSLKDQALEQYLATEQARRSVQISLVAEVASNYLTLAADRERLKLA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHH
QDTLASQQSSYNLTKSRFEAGVSSFLDLNQARTSVESARVDIARYTTLIAQDVNALNLVV
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
GSPVSTALLPAALTENLSAMKDVAPGLPSDVLLRRPDILQAENLLKGANANIGAARANFF
CCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHCCCCCCCCCHHHCCC
PRITLLSSVGFGSDELTGLFSGNSFTWNFAPRITLPIFTAGANQATLKVAEVDRDIAVAQ
HHHHHHHHCCCCCCCCEEEECCCEEEEECCCEEEEEEEECCCCCCEEEEECCCCHHHHHH
YEKAIQTAFREVADALAQRGTIDDQLSAQQSLTDATSESYRLSQARYEKGVDSYLQVLDS
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QRALYGAQQNLIGVRLVRLLNVATLYKVLGGGAPGQE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]