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The map label for this gene is mexA [H]

Identifier: 78222029

GI number: 78222029

Start: 888033

End: 889211

Strand: Direct

Name: mexA [H]

Synonym: Gmet_0809

Alternate gene names: 78222029

Gene position: 888033-889211 (Clockwise)

Preceding gene: 78222028

Following gene: 78222030

Centisome position: 22.22

GC content: 63.95

Gene sequence:

>1179_bases
ATGTACAGAACACGACTGATTACCGTTACGGGGCTTCTTGCCATCGGGATGATGGTGGCAGGGTGCGGCAAGAAATCCAA
CGGCGCCCCTCCTCCCAGCGGTCCTCCCGAAGTGGGAGTGGTTACGATTCAGCCCCAACGGGTGGAGCTGACGACTGAGT
TGCCGGGCCGCACCTCGCCTCACCTGATCGCCGAGGTCCGTCCCCAGGTCAGCGGCATCATCCAGAAGCGCCTGTTCACC
GAGGGATCCGACGTGAAGGCGGGTCAGGTCCTGTACCAGATTGACCCCTCCACTTATCAGGCGGCCTTTGCCAGCGCCAA
GGCCACCCTTGCCCGGACGGAAGCCAATCTCATCCCGGCGCGCCTCAAGGAAGAGCGCTTCCGTGATCTGGTGAAGATCA
AGGCCGTGAGCCAGCAGGACTACGACGACGCCAACGCCGCCCTCAAGCAGGCCGAGGCCGACGTCGCCTCTGCCAAGGCA
GCGGTTGAGACGGCCCGCATCAACTTGGGCTACACGAAGGTGACCGCCCCCATCTCCGGCCGGATCGGCCGTTCCACCGT
GACCGACGGGGCTCTGGTGACGGCCAACCAGCCCACGGCCCTGGCCACCATCCAGCAGCTCAGTTCCATGTATGTGGATG
TCACCCAGTCCAACGCCGATCTGTTGAAGCTGAAGCAGAACCTGGCCAGCGGACTGATGAAACACGACGGTGCCGCCCAG
GCGCGGGTCAAGCTTCTGCTGGAGGACGGCAGCCCTTATCCGCTGTCGGGAACCCTGAAATTCTCGGAAGTCACCGTTGA
CCAGAGCACCGGTTCCATCACCCTGCGGGCGGTCTTCCCGAACCCGAAGCAAACCCTCCTGCCGGGGATGTTCGTGCGGG
CCATCGTTGAGGATGGTGTCAGCGAACAAGCGATCCTGGTCCCCCAGCGGGGCGTCACCCGTAACCCGAAGGGCGATGCC
ACGGCTCTGGTGGTGGGGGCCGAGAACAAGGTGGAGTCACGGGTCATCAAGGTCGCCCGGACCGTTGGGGACAACTGGCT
GGTGAGCGACGGGCTCAAGGCCGGTGATCGGGTCATCCTGGAAGGGATTCAGAAGGCAAAGCCGGGCACGCCGGTCAAGG
CGGTGCCGTTCGGAGCAAAGCCGGAGACGGCTCCGGCCCCAGCCGTCGTGAAAAAATAA

Upstream 100 bases:

>100_bases
GACCATCGAGGCCTGCTGGGATGCGGTCAGGCGATAGGCTGAATCAAGTTTAGGAATGAACATTCATTCAAATTACCGTG
AAGAGGTGTCTATGCAAGCC

Downstream 100 bases:

>100_bases
CCTGAAAGTTCACCACGGTGGCACGGAGCACGGAGAATTCAGTTTTTGAGCAATTAGAAGTTTGTTTTTTGCCTTTCTCT
GTGTCTCCGTGTCGCCGTTA

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MYRTRLITVTGLLAIGMMVAGCGKKSNGAPPPSGPPEVGVVTIQPQRVELTTELPGRTSPHLIAEVRPQVSGIIQKRLFT
EGSDVKAGQVLYQIDPSTYQAAFASAKATLARTEANLIPARLKEERFRDLVKIKAVSQQDYDDANAALKQAEADVASAKA
AVETARINLGYTKVTAPISGRIGRSTVTDGALVTANQPTALATIQQLSSMYVDVTQSNADLLKLKQNLASGLMKHDGAAQ
ARVKLLLEDGSPYPLSGTLKFSEVTVDQSTGSITLRAVFPNPKQTLLPGMFVRAIVEDGVSEQAILVPQRGVTRNPKGDA
TALVVGAENKVESRVIKVARTVGDNWLVSDGLKAGDRVILEGIQKAKPGTPVKAVPFGAKPETAPAPAVVKK

Sequences:

>Translated_392_residues
MYRTRLITVTGLLAIGMMVAGCGKKSNGAPPPSGPPEVGVVTIQPQRVELTTELPGRTSPHLIAEVRPQVSGIIQKRLFT
EGSDVKAGQVLYQIDPSTYQAAFASAKATLARTEANLIPARLKEERFRDLVKIKAVSQQDYDDANAALKQAEADVASAKA
AVETARINLGYTKVTAPISGRIGRSTVTDGALVTANQPTALATIQQLSSMYVDVTQSNADLLKLKQNLASGLMKHDGAAQ
ARVKLLLEDGSPYPLSGTLKFSEVTVDQSTGSITLRAVFPNPKQTLLPGMFVRAIVEDGVSEQAILVPQRGVTRNPKGDA
TALVVGAENKVESRVIKVARTVGDNWLVSDGLKAGDRVILEGIQKAKPGTPVKAVPFGAKPETAPAPAVVKK
>Mature_392_residues
MYRTRLITVTGLLAIGMMVAGCGKKSNGAPPPSGPPEVGVVTIQPQRVELTTELPGRTSPHLIAEVRPQVSGIIQKRLFT
EGSDVKAGQVLYQIDPSTYQAAFASAKATLARTEANLIPARLKEERFRDLVKIKAVSQQDYDDANAALKQAEADVASAKA
AVETARINLGYTKVTAPISGRIGRSTVTDGALVTANQPTALATIQQLSSMYVDVTQSNADLLKLKQNLASGLMKHDGAAQ
ARVKLLLEDGSPYPLSGTLKFSEVTVDQSTGSITLRAVFPNPKQTLLPGMFVRAIVEDGVSEQAILVPQRGVTRNPKGDA
TALVVGAENKVESRVIKVARTVGDNWLVSDGLKAGDRVILEGIQKAKPGTPVKAVPFGAKPETAPAPAVVKK

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=363, Percent_Identity=57.3002754820937, Blast_Score=397, Evalue=1e-112,
Organism=Escherichia coli, GI1786668, Length=368, Percent_Identity=57.8804347826087, Blast_Score=396, Evalue=1e-111,
Organism=Escherichia coli, GI1789929, Length=377, Percent_Identity=49.6021220159151, Blast_Score=347, Evalue=6e-97,
Organism=Escherichia coli, GI87082036, Length=321, Percent_Identity=28.9719626168224, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI87081796, Length=332, Percent_Identity=29.2168674698795, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789637, Length=157, Percent_Identity=31.2101910828025, Blast_Score=65, Evalue=9e-12,
Organism=Escherichia coli, GI87081951, Length=147, Percent_Identity=31.2925170068027, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 41441; Mature: 41441

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRTRLITVTGLLAIGMMVAGCGKKSNGAPPPSGPPEVGVVTIQPQRVELTTELPGRTSP
CCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCC
HLIAEVRPQVSGIIQKRLFTEGSDVKAGQVLYQIDPSTYQAAFASAKATLARTEANLIPA
CHHHHHCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCHH
RLKEERFRDLVKIKAVSQQDYDDANAALKQAEADVASAKAAVETARINLGYTKVTAPISG
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEECCCCC
RIGRSTVTDGALVTANQPTALATIQQLSSMYVDVTQSNADLLKLKQNLASGLMKHDGAAQ
CCCCCCCCCCEEEECCCCHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCCC
ARVKLLLEDGSPYPLSGTLKFSEVTVDQSTGSITLRAVFPNPKQTLLPGMFVRAIVEDGV
EEEEEEEECCCCCCCCCEEEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCC
SEQAILVPQRGVTRNPKGDATALVVGAENKVESRVIKVARTVGDNWLVSDGLKAGDRVIL
CCCEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHH
EGIQKAKPGTPVKAVPFGAKPETAPAPAVVKK
HHHHHCCCCCCEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MYRTRLITVTGLLAIGMMVAGCGKKSNGAPPPSGPPEVGVVTIQPQRVELTTELPGRTSP
CCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCCCCC
HLIAEVRPQVSGIIQKRLFTEGSDVKAGQVLYQIDPSTYQAAFASAKATLARTEANLIPA
CHHHHHCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCHH
RLKEERFRDLVKIKAVSQQDYDDANAALKQAEADVASAKAAVETARINLGYTKVTAPISG
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEECCCCC
RIGRSTVTDGALVTANQPTALATIQQLSSMYVDVTQSNADLLKLKQNLASGLMKHDGAAQ
CCCCCCCCCCEEEECCCCHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCCC
ARVKLLLEDGSPYPLSGTLKFSEVTVDQSTGSITLRAVFPNPKQTLLPGMFVRAIVEDGV
EEEEEEEECCCCCCCCCEEEEEEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCC
SEQAILVPQRGVTRNPKGDATALVVGAENKVESRVIKVARTVGDNWLVSDGLKAGDRVIL
CCCEEEECCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHH
EGIQKAKPGTPVKAVPFGAKPETAPAPAVVKK
HHHHHCCCCCCEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]