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The map label for this gene is zraS [H]

Identifier: 78221916

GI number: 78221916

Start: 763433

End: 764179

Strand: Direct

Name: zraS [H]

Synonym: Gmet_0696

Alternate gene names: 78221916

Gene position: 763433-764179 (Clockwise)

Preceding gene: 78221915

Following gene: 78221917

Centisome position: 19.1

GC content: 64.52

Gene sequence:

>747_bases
ATGGCGGATGCACTGGCGAAATATCTCCCCGACAACGGCAACGTGGAAGGGGAACTTCTGGAACTGCTTGCCTTCAACGA
GAAGATGGCGGAACTGGGGAGGATGGCGGCGGGAGTGGTTCACGAGCTGAACACCCCCCTGTCGGTCATTGTCTCGGCCA
CCCAGATGATCCTCCGGGAGGAGGAACTGCCCGAGTTCGTCCGCGAGATGGTGGAGCGGGTCAACGAGGAGGCCCACCGC
CTGGCCGAGCTGACCAAGGGGGTCCTCTCCTTTGCCCGGCGGGATAGTGGGGGGCGGGCCGAGGCGGATGCGAACCAGGT
CATCGGCGAGGTCATGACCTTCCTCCGCTTCGAGGCCGGGAAGCGGTCCATCGGTGTCATCGAGGACCTGGACCACCGGC
TCCCCTTCATCGCCGCCGACGCCAACCGGGTGAAACAGGTGATCATCAACCTGGTCATGAACGCCTTCCAGGCCATGGGT
GAGGGGGGAACTCTCTTCCTGCGGACGGTTCAGGAGAGCGAGACGGCCGTTACGGTGCAGGTGGCCGACACCGGTCCCGG
CATCCCCCCTGACGCCCAGGAGCGGATCTTTACCCCCTTCTTCACCACCAAGGAGCCGGGGGAGGGGACCGGCCTCGGCC
TCTTCATCACCCGCAAGCTCATGGAAGGAATCGGCGGCGCCATCGCCGTGAAGAGCGTGGTGGGAGAGGGGACCACCTTC
ACCCTGACCTTTCCCGTTGCGGAATAA

Upstream 100 bases:

>100_bases
TTGCTTTGTAACTTGTTGATAATACGAATGGTGCTTCTGGCACGCCTCGTGCCTACAAGGTATGAGGCACCCTATTTAAC
CGGTCTGCACCAGAGGACCC

Downstream 100 bases:

>100_bases
CCCTTTAAAAAGCCCAATAACACGGAGAGACGGAGAGCACGGAGTTTTCTTCACACGTTGTCGTGTTGTCGAGGGGTAGG
TGTTACTCCCGTTGGCTTGT

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MADALAKYLPDNGNVEGELLELLAFNEKMAELGRMAAGVVHELNTPLSVIVSATQMILREEELPEFVREMVERVNEEAHR
LAELTKGVLSFARRDSGGRAEADANQVIGEVMTFLRFEAGKRSIGVIEDLDHRLPFIAADANRVKQVIINLVMNAFQAMG
EGGTLFLRTVQESETAVTVQVADTGPGIPPDAQERIFTPFFTTKEPGEGTGLGLFITRKLMEGIGGAIAVKSVVGEGTTF
TLTFPVAE

Sequences:

>Translated_248_residues
MADALAKYLPDNGNVEGELLELLAFNEKMAELGRMAAGVVHELNTPLSVIVSATQMILREEELPEFVREMVERVNEEAHR
LAELTKGVLSFARRDSGGRAEADANQVIGEVMTFLRFEAGKRSIGVIEDLDHRLPFIAADANRVKQVIINLVMNAFQAMG
EGGTLFLRTVQESETAVTVQVADTGPGIPPDAQERIFTPFFTTKEPGEGTGLGLFITRKLMEGIGGAIAVKSVVGEGTTF
TLTFPVAE
>Mature_247_residues
ADALAKYLPDNGNVEGELLELLAFNEKMAELGRMAAGVVHELNTPLSVIVSATQMILREEELPEFVREMVERVNEEAHRL
AELTKGVLSFARRDSGGRAEADANQVIGEVMTFLRFEAGKRSIGVIEDLDHRLPFIAADANRVKQVIINLVMNAFQAMGE
GGTLFLRTVQESETAVTVQVADTGPGIPPDAQERIFTPFFTTKEPGEGTGLGLFITRKLMEGIGGAIAVKSVVGEGTTFT
LTFPVAE

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=221, Percent_Identity=36.1990950226244, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1788549, Length=224, Percent_Identity=33.0357142857143, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1790300, Length=222, Percent_Identity=32.4324324324324, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1789149, Length=217, Percent_Identity=29.9539170506912, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI145693157, Length=242, Percent_Identity=29.3388429752066, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI48994928, Length=224, Percent_Identity=26.7857142857143, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI87081816, Length=217, Percent_Identity=26.2672811059908, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786783, Length=257, Percent_Identity=27.6264591439689, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790346, Length=231, Percent_Identity=25.5411255411255, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1788713, Length=116, Percent_Identity=31.0344827586207, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1786600, Length=218, Percent_Identity=26.1467889908257, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1786912, Length=220, Percent_Identity=29.0909090909091, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1789808, Length=240, Percent_Identity=28.3333333333333, Blast_Score=62, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=93, Percent_Identity=40.8602150537634, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 26862; Mature: 26731

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADALAKYLPDNGNVEGELLELLAFNEKMAELGRMAAGVVHELNTPLSVIVSATQMILRE
CCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
EELPEFVREMVERVNEEAHRLAELTKGVLSFARRDSGGRAEADANQVIGEVMTFLRFEAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCC
KRSIGVIEDLDHRLPFIAADANRVKQVIINLVMNAFQAMGEGGTLFLRTVQESETAVTVQ
CCCCCHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEE
VADTGPGIPPDAQERIFTPFFTTKEPGEGTGLGLFITRKLMEGIGGAIAVKSVVGEGTTF
EECCCCCCCCCCHHCEECCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCEE
TLTFPVAE
EEEECCCC
>Mature Secondary Structure 
ADALAKYLPDNGNVEGELLELLAFNEKMAELGRMAAGVVHELNTPLSVIVSATQMILRE
CCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
EELPEFVREMVERVNEEAHRLAELTKGVLSFARRDSGGRAEADANQVIGEVMTFLRFEAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCC
KRSIGVIEDLDHRLPFIAADANRVKQVIINLVMNAFQAMGEGGTLFLRTVQESETAVTVQ
CCCCCHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEEE
VADTGPGIPPDAQERIFTPFFTTKEPGEGTGLGLFITRKLMEGIGGAIAVKSVVGEGTTF
EECCCCCCCCCCHHCEECCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCEE
TLTFPVAE
EEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]