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The map label for this gene is amtB [C]

Identifier: 78221915

GI number: 78221915

Start: 761746

End: 763212

Strand: Direct

Name: amtB [C]

Synonym: Gmet_0695

Alternate gene names: 78221915

Gene position: 761746-763212 (Clockwise)

Preceding gene: 78221914

Following gene: 78221916

Centisome position: 19.06

GC content: 64.55

Gene sequence:

>1467_bases
ATGAAGTTTAAACTATCATTTGTCGCATTGCTGGCAACTGTCGCGCTCTCGCTCCCGGTCGCCCTCCTGGCAGAGGAGGT
GAAGACCGCCGCTCCGGCACAGGCTGCCCTGTCCTCGGCCCAGACGGCTGCCCTTGCGCCCGCTGTCACCGCGGCGCCCG
CCGCTGCTCCGGCCACCCCTGCCGCACCGAAAAACGTCGATCCGGTTCTCAATACCGGCGACACCGCCTGGATGCTCGTC
TCCGCCGCCATGGTCCTCTTCATGATTCCGGGCCTTGCCTTCTTCTACGGCGGCATGGTCCGGGGCAAGAACGTCCTCTC
GACGATCATGCACTCCTTTGTCGCCATGGGGATCGTCGGTGTGCAATGGGCGGTGATTGGCTACTCCCTCTCCTTCGGCC
CTGACATCGGCGGCGGCCTCCTCGGGAACCTGAGCAAGGCGCTCCTGAACGGTCTCATCACCTTCAAGGATGGCAACCCG
GTCTACGCCCTCTTTCAGAACGTGCCGACCGCACCGGGGGCCATCCCTGAGTACGTCTTCGCCATGTTCCAGTGCATGTT
CGCCATGATTACCGTTGCCCTCATCTCCGGCGCCCTGGCCGAGCGGGTGAAGTTCTCCGCCTACTGCGTCTTCGTGCTCC
TCTGGACCACCCTGGTGTACGACCCCTTGGCCCACTGGGTCTGGATGAGCGACGGCTGGCTCTTCAAGAAAGGCGCTCTC
GACTTCGCCGGCGGCACGGTTGTTCACCTCTCCTCCGGTATCTCGGCCCTGGCGTTTCTCATCTTCCTCGGCAAGCGGCA
CGGGTTTCCCCACGAGCGGATGGCGCCCCACAGCCTGCCGCTGACTATGCTCGGCGTCGGCATGCTCTGGTTCGGCTGGT
TCGGCTTCAACGCCGGTTCCGCCATCGTCGGGGCCAACTGCTCCGACGCGGCGGGGGGGCTGGCCGGTCTCGCCTTTGCC
ACCACGACCATCGCGCCGGCTGCCGGCGGCCTTTCCTGGATGATCGCGGAATGGATTCACGCGGGCAAACCTTCCGCCCT
CGGTTTCGGTTCCGGTGTCGTTGCCGGCCTGGTCGTCATTACCCCCGCTGCCGGTTTCGTGCAACCCGGGGCCGCCATCC
TCATGGGACTTGCGGCGGGCGTGGTCTGCTATGGTGGCGTGCTGCTGAAGGCCAAGCTCAAGTACGACGACTCCCTCGAC
GCCTTCGGTGTCCACGGAGTGGGGGGGACCTTCGGCGCCCTCACCACCGGTATCTTCGCCACCGTCGGCGCCACGGGGCT
CCTCTCCGGCAATGTCTCCCAGTTCTTAACCCAGGTGATTGCAGTCGTGGCCGCCGGCGCCTATGCCTTCATCGTCACCC
TTGTTATTGCCTTTGTCCTCCACAAGACCATCGGGCTCCGCGTGGAAAAGGAAGACGAGATCATGGGGCTCGACCAGACC
CAGCATTCCGAGTCGGCCTACAACTGA

Upstream 100 bases:

>100_bases
TTCATCCTTCCCCTCGACGAGGCGGTCAGGATCAGGACCGGCGAAAAGGGTGCCGAGGCCATCTGAGAGGCAGGAATAGA
TCAGTCCATGGAGGTAACCA

Downstream 100 bases:

>100_bases
GCGTTTTGCCGATTCACTCGCAACATTGAAGCCGCCTCTATCGGGGCGGCTTTCTTTGTGCCTCAATTGTGGGCGCTTTG
CTGCCTAAATGGTGTGCATG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MKFKLSFVALLATVALSLPVALLAEEVKTAAPAQAALSSAQTAALAPAVTAAPAAAPATPAAPKNVDPVLNTGDTAWMLV
SAAMVLFMIPGLAFFYGGMVRGKNVLSTIMHSFVAMGIVGVQWAVIGYSLSFGPDIGGGLLGNLSKALLNGLITFKDGNP
VYALFQNVPTAPGAIPEYVFAMFQCMFAMITVALISGALAERVKFSAYCVFVLLWTTLVYDPLAHWVWMSDGWLFKKGAL
DFAGGTVVHLSSGISALAFLIFLGKRHGFPHERMAPHSLPLTMLGVGMLWFGWFGFNAGSAIVGANCSDAAGGLAGLAFA
TTTIAPAAGGLSWMIAEWIHAGKPSALGFGSGVVAGLVVITPAAGFVQPGAAILMGLAAGVVCYGGVLLKAKLKYDDSLD
AFGVHGVGGTFGALTTGIFATVGATGLLSGNVSQFLTQVIAVVAAGAYAFIVTLVIAFVLHKTIGLRVEKEDEIMGLDQT
QHSESAYN

Sequences:

>Translated_488_residues
MKFKLSFVALLATVALSLPVALLAEEVKTAAPAQAALSSAQTAALAPAVTAAPAAAPATPAAPKNVDPVLNTGDTAWMLV
SAAMVLFMIPGLAFFYGGMVRGKNVLSTIMHSFVAMGIVGVQWAVIGYSLSFGPDIGGGLLGNLSKALLNGLITFKDGNP
VYALFQNVPTAPGAIPEYVFAMFQCMFAMITVALISGALAERVKFSAYCVFVLLWTTLVYDPLAHWVWMSDGWLFKKGAL
DFAGGTVVHLSSGISALAFLIFLGKRHGFPHERMAPHSLPLTMLGVGMLWFGWFGFNAGSAIVGANCSDAAGGLAGLAFA
TTTIAPAAGGLSWMIAEWIHAGKPSALGFGSGVVAGLVVITPAAGFVQPGAAILMGLAAGVVCYGGVLLKAKLKYDDSLD
AFGVHGVGGTFGALTTGIFATVGATGLLSGNVSQFLTQVIAVVAAGAYAFIVTLVIAFVLHKTIGLRVEKEDEIMGLDQT
QHSESAYN
>Mature_488_residues
MKFKLSFVALLATVALSLPVALLAEEVKTAAPAQAALSSAQTAALAPAVTAAPAAAPATPAAPKNVDPVLNTGDTAWMLV
SAAMVLFMIPGLAFFYGGMVRGKNVLSTIMHSFVAMGIVGVQWAVIGYSLSFGPDIGGGLLGNLSKALLNGLITFKDGNP
VYALFQNVPTAPGAIPEYVFAMFQCMFAMITVALISGALAERVKFSAYCVFVLLWTTLVYDPLAHWVWMSDGWLFKKGAL
DFAGGTVVHLSSGISALAFLIFLGKRHGFPHERMAPHSLPLTMLGVGMLWFGWFGFNAGSAIVGANCSDAAGGLAGLAFA
TTTIAPAAGGLSWMIAEWIHAGKPSALGFGSGVVAGLVVITPAAGFVQPGAAILMGLAAGVVCYGGVLLKAKLKYDDSLD
AFGVHGVGGTFGALTTGIFATVGATGLLSGNVSQFLTQVIAVVAAGAYAFIVTLVIAFVLHKTIGLRVEKEDEIMGLDQT
QHSESAYN

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=459, Percent_Identity=42.7015250544662, Blast_Score=309, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI17550182, Length=451, Percent_Identity=31.0421286031042, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17550180, Length=438, Percent_Identity=31.2785388127854, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI133931100, Length=457, Percent_Identity=26.6958424507659, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI32564042, Length=439, Percent_Identity=26.4236902050114, Blast_Score=125, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6324187, Length=448, Percent_Identity=35.9375, Blast_Score=249, Evalue=5e-67,
Organism=Saccharomyces cerevisiae, GI6325396, Length=431, Percent_Identity=33.8747099767981, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6321559, Length=426, Percent_Identity=32.1596244131455, Blast_Score=220, Evalue=4e-58,
Organism=Drosophila melanogaster, GI161078314, Length=431, Percent_Identity=31.0904872389791, Blast_Score=153, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 50257; Mature: 50257

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFKLSFVALLATVALSLPVALLAEEVKTAAPAQAALSSAQTAALAPAVTAAPAAAPATP
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCC
AAPKNVDPVLNTGDTAWMLVSAAMVLFMIPGLAFFYGGMVRGKNVLSTIMHSFVAMGIVG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHH
VQWAVIGYSLSFGPDIGGGLLGNLSKALLNGLITFKDGNPVYALFQNVPTAPGAIPEYVF
HHHHHHEEEEECCCCCCCHHHHHHHHHHHCCEEEEECCCCEEHHHHCCCCCCCCHHHHHH
AMFQCMFAMITVALISGALAERVKFSAYCVFVLLWTTLVYDPLAHWVWMSDGWLFKKGAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCC
DFAGGTVVHLSSGISALAFLIFLGKRHGFPHERMAPHSLPLTMLGVGMLWFGWFGFNAGS
CCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
AIVGANCSDAAGGLAGLAFATTTIAPAAGGLSWMIAEWIHAGKPSALGFGSGVVAGLVVI
EEEECCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
TPAAGFVQPGAAILMGLAAGVVCYGGVLLKAKLKYDDSLDAFGVHGVGGTFGALTTGIFA
CCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
TVGATGLLSGNVSQFLTQVIAVVAAGAYAFIVTLVIAFVLHKTIGLRVEKEDEIMGLDQT
HHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCC
QHSESAYN
CCCCCCCC
>Mature Secondary Structure
MKFKLSFVALLATVALSLPVALLAEEVKTAAPAQAALSSAQTAALAPAVTAAPAAAPATP
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHCCCCCCCCCC
AAPKNVDPVLNTGDTAWMLVSAAMVLFMIPGLAFFYGGMVRGKNVLSTIMHSFVAMGIVG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHHHHHHHHHHHHHHH
VQWAVIGYSLSFGPDIGGGLLGNLSKALLNGLITFKDGNPVYALFQNVPTAPGAIPEYVF
HHHHHHEEEEECCCCCCCHHHHHHHHHHHCCEEEEECCCCEEHHHHCCCCCCCCHHHHHH
AMFQCMFAMITVALISGALAERVKFSAYCVFVLLWTTLVYDPLAHWVWMSDGWLFKKGAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCC
DFAGGTVVHLSSGISALAFLIFLGKRHGFPHERMAPHSLPLTMLGVGMLWFGWFGFNAGS
CCCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
AIVGANCSDAAGGLAGLAFATTTIAPAAGGLSWMIAEWIHAGKPSALGFGSGVVAGLVVI
EEEECCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
TPAAGFVQPGAAILMGLAAGVVCYGGVLLKAKLKYDDSLDAFGVHGVGGTFGALTTGIFA
CCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHH
TVGATGLLSGNVSQFLTQVIAVVAAGAYAFIVTLVIAFVLHKTIGLRVEKEDEIMGLDQT
HHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCC
QHSESAYN
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]