Click here to switch to the map view.

The map label for this gene is nifE [H]

Identifier: 78221889

GI number: 78221889

Start: 733828

End: 736587

Strand: Direct

Name: nifE [H]

Synonym: Gmet_0669

Alternate gene names: 78221889

Gene position: 733828-736587 (Clockwise)

Preceding gene: 78221885

Following gene: 78221890

Centisome position: 18.36

GC content: 65.36

Gene sequence:

>2760_bases
ATGGCCAAACCAGACTACTACGACGTATCCGAATGCGAAACCCACGAAAAAGGTGCCCCCAAGTTCTGCAAGAAATCGGA
GCCGGGGGAGGGGGCCGAGCGCTCCTGTGCCTACGACGGCGCCCGGGTGGTGCTCATGCCGATCACCGATGTGATCCATC
TGGTGCACGGCCCCATCGCCTGCGCCGGCAATTCCTGGGACAACCGGGGGGCCCGCTCCTCGGACTCCCAGCTCTACCGC
CGGGGTTTCACAACCGAGATGCTGGAGAACGACGTGGTTTTCGGTGGCGAGAAGAAGCTCTACCGGGCCATCCTGGAGCT
GGCCGAGCGCTACGAAGGCCAGGCGAAGGCCATGTTCGTCTACGCCACCTGCGTCACCGCCATGACCGGCGACGACGTGG
AGGCGGTCTGCGCCGCGGCCGGCAAGAAGGTCGCCATCCCCCTCATCCCCGTCAACACCCCCGGTTTCATCGGCGACAAG
AACATCGGCAACCGCTTGGCCGGCGAGGTGCTCTTCAAGCATGTCATCGGCACCGCCGAGCCGCCGGTTCTGGGGGAGTA
TCCGATAAATCTCATTGGCGAGTACAACATTGCCGGCGACCTCTGGGGGATGCTGCCGCTCTTTGAGCGCCTCGGCATCC
AGGTCCTCTCCTGCTTCAGCGGCGACGCCACCTTCGAGGAACTCCGCTACGCCCACCGGGCAAAGCTCAATATTATCATC
TGCTCCAAGAGCCTCACCAACCTCGCCAGGAAGATGCAGAAGAACTACGGCATGCCCTATCTGGAGGAGTCCTTCTACGG
CATGACCGACACGGCCAAAGCCCTGCGGGACATCGCCCGGGAGCTGGACGACGCCGTGGGGGGGCTGGAGAAGCGGATCA
TGCAGGACCGGGTGGAGAAGCTACTGGAGGAGGAAGAGGCGACGTGCCGCGAGCGCCTTGCCCCCTATCGGGCGCGGCTG
GAGGGGAAGCGGTCGGTCCTCTTCACCGGCGGGGTCAAGACCTGGTCCATGGTGAACGCCCTGCGGGAGCTGGGGGTGGA
GATCCTGGCCGCCGGCACCCAGAACTCCACCCTGGAGGACTTCTACCGGATGAAGGCCCTCATGCACCAGGATGCCCGGA
TCATCGAGGACACCTCCAGCGCCGGGCTCCTCCAGGTCATGTACGACAAGATGCCGGATCTCATTGTAGCCGGGGGGAAG
ACCAAGTTCCTGGCCCTTAAGACCAAGACCCCCTTCCTGGATATCAACCACGGCCGCTCCCACCCCTACGCCGGGTACGA
GGGGATGGTGACCTTTGCGAAGCAGCTGGACCTCACGGTCAACAACCCCATCTGGCCGGTGCTGAACGCCAAGGCGCCCT
GGGAGAAGACGGAGGAAGAGTTGACGGCCGCGGTGGCGCTTGCCGCCGGTCACGCCCGAGCCTGCCTGGATGAGGATCTG
AAGGATTCCACGGTGAAGGTGCCGGCCAAGAACGCCACGGTGAACCCCCAGAAGAACTCCCCGGCCCTTGGCGCGACCCT
GGCCTATCTCGGCATCGACCAGATGCTGGCCCTTCTCCATGGCGCCCAGGGATGCTCCACCTTCATCCGGCTGCAGCTCT
CGCGCCATTTCAAGGAGCCCGTCGCCCTGAACTCCACCGCCATGAGCGAGGACACCGCCATCTTCGGCGGGTGGGAGAAC
CTGAAAAAGGGGCTGAAAAAGGTGATCGAGAAGTTCAGCCCGGAGGTGGTGGGGGTCATGACCTCGGGGCTCACCGAGAC
CATGGGGGATGATGTCCGGAGCGCCATCGTCCACTTTCGGCAGGAGTACCCCGAGCACGACGGGGTCCCCGTCGTTTGGG
CTTCGACCCCTGACTACTGCGGCTCGCTGCAGGAGGGGTACGCGGCAACGGTGGAGGCCATCGTGAGGAGCGTCCCCGAG
CCGGGGGAGACGATCCCGGGCCAGGTGACGGTCCTGCCCGGTGCCCACCTGACCCCGGCCGACGTGGAGGAGGTGCGGGA
GCTCTGCGAGGCCTTCGGGCTCGACCCCATCATCGTCCCCGACATCGCCAACGCCCTGGATGGCCACATCGACGAGACAG
TGTCGCCGCTTTCAACCGGCGGGGTTTCCATGGCCCGGATCAGGCAGGCGGGGCAAAGCGCGGCGACCCTCTTCATCGGC
GATTCCCTGGCCAAGGCTGCCGAGGCCATGACGGAGCGGTGCGGCATGCCCAGCTATGGCTTCACCTCCCTCACGGGGCT
TGCCCAGGTGGACCGCTTCATGGAGACCCTCGCGGCCATCGCGGGGCGGCCGATCCCCGAGAAGTTCCGCCGCTGGCGGA
GCCGCCTCATGGACGCCATGGTGGACAGCCACTACCAGTTCGGCCTCAAGAAAGTCACGGTGGCCCTGGAGGGAGACAAC
CTGAAAACGCTGGTGAACTTCCTGGCGGGGATGGGGTGCGAGATACAGGCGGCCATCGCGGCGACACGGGTCCGGGGGCT
CGACGGGCTGCCGGCTAGGGATATCTTCGTGGGGGATCTGGAGGACCTGGAAACGGCGGCTAGGGGAAGCGACCTGATCG
TGGCCAACTCCAATGGCCGTCAGGCCGCGGCAAAGCTGGGGATCAAGGCCCACCTGCGGGCGGGGCTTCCGGTCTTCGAT
CGCCTTGGCGCCCACCAGAAGATGTGGGTGGGGTACCGGGGGACCATGAACCTTCTGTTCGAGACGGCGAACCTGTTCCA
GGCCAACGCGGGAGAGGGGCAGAAGCTGGCGCATAACTGA

Upstream 100 bases:

>100_bases
CAACACGGAAGATGTATCCCGACGGTTTTCAGGCAAAGGCGCCGACCATTCCCCCCCCCGGAATGGCGGCGCCTTTTTTC
TTTGTGCATTCGAGGTTGCC

Downstream 100 bases:

>100_bases
AAAGCTGTAATAACACAGAGCGACAGAGCAACGGAGAAAACCTCAATAACTGAATTTAGACGTCTTCAGGAGGAAATTGC
GTTTTTCTCTGTTGCTCAGT

Product: bifunctional nitrogenase molybdenum-cofactor biosynthesis protein NifE/NifN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 919; Mature: 918

Protein sequence:

>919_residues
MAKPDYYDVSECETHEKGAPKFCKKSEPGEGAERSCAYDGARVVLMPITDVIHLVHGPIACAGNSWDNRGARSSDSQLYR
RGFTTEMLENDVVFGGEKKLYRAILELAERYEGQAKAMFVYATCVTAMTGDDVEAVCAAAGKKVAIPLIPVNTPGFIGDK
NIGNRLAGEVLFKHVIGTAEPPVLGEYPINLIGEYNIAGDLWGMLPLFERLGIQVLSCFSGDATFEELRYAHRAKLNIII
CSKSLTNLARKMQKNYGMPYLEESFYGMTDTAKALRDIARELDDAVGGLEKRIMQDRVEKLLEEEEATCRERLAPYRARL
EGKRSVLFTGGVKTWSMVNALRELGVEILAAGTQNSTLEDFYRMKALMHQDARIIEDTSSAGLLQVMYDKMPDLIVAGGK
TKFLALKTKTPFLDINHGRSHPYAGYEGMVTFAKQLDLTVNNPIWPVLNAKAPWEKTEEELTAAVALAAGHARACLDEDL
KDSTVKVPAKNATVNPQKNSPALGATLAYLGIDQMLALLHGAQGCSTFIRLQLSRHFKEPVALNSTAMSEDTAIFGGWEN
LKKGLKKVIEKFSPEVVGVMTSGLTETMGDDVRSAIVHFRQEYPEHDGVPVVWASTPDYCGSLQEGYAATVEAIVRSVPE
PGETIPGQVTVLPGAHLTPADVEEVRELCEAFGLDPIIVPDIANALDGHIDETVSPLSTGGVSMARIRQAGQSAATLFIG
DSLAKAAEAMTERCGMPSYGFTSLTGLAQVDRFMETLAAIAGRPIPEKFRRWRSRLMDAMVDSHYQFGLKKVTVALEGDN
LKTLVNFLAGMGCEIQAAIAATRVRGLDGLPARDIFVGDLEDLETAARGSDLIVANSNGRQAAAKLGIKAHLRAGLPVFD
RLGAHQKMWVGYRGTMNLLFETANLFQANAGEGQKLAHN

Sequences:

>Translated_919_residues
MAKPDYYDVSECETHEKGAPKFCKKSEPGEGAERSCAYDGARVVLMPITDVIHLVHGPIACAGNSWDNRGARSSDSQLYR
RGFTTEMLENDVVFGGEKKLYRAILELAERYEGQAKAMFVYATCVTAMTGDDVEAVCAAAGKKVAIPLIPVNTPGFIGDK
NIGNRLAGEVLFKHVIGTAEPPVLGEYPINLIGEYNIAGDLWGMLPLFERLGIQVLSCFSGDATFEELRYAHRAKLNIII
CSKSLTNLARKMQKNYGMPYLEESFYGMTDTAKALRDIARELDDAVGGLEKRIMQDRVEKLLEEEEATCRERLAPYRARL
EGKRSVLFTGGVKTWSMVNALRELGVEILAAGTQNSTLEDFYRMKALMHQDARIIEDTSSAGLLQVMYDKMPDLIVAGGK
TKFLALKTKTPFLDINHGRSHPYAGYEGMVTFAKQLDLTVNNPIWPVLNAKAPWEKTEEELTAAVALAAGHARACLDEDL
KDSTVKVPAKNATVNPQKNSPALGATLAYLGIDQMLALLHGAQGCSTFIRLQLSRHFKEPVALNSTAMSEDTAIFGGWEN
LKKGLKKVIEKFSPEVVGVMTSGLTETMGDDVRSAIVHFRQEYPEHDGVPVVWASTPDYCGSLQEGYAATVEAIVRSVPE
PGETIPGQVTVLPGAHLTPADVEEVRELCEAFGLDPIIVPDIANALDGHIDETVSPLSTGGVSMARIRQAGQSAATLFIG
DSLAKAAEAMTERCGMPSYGFTSLTGLAQVDRFMETLAAIAGRPIPEKFRRWRSRLMDAMVDSHYQFGLKKVTVALEGDN
LKTLVNFLAGMGCEIQAAIAATRVRGLDGLPARDIFVGDLEDLETAARGSDLIVANSNGRQAAAKLGIKAHLRAGLPVFD
RLGAHQKMWVGYRGTMNLLFETANLFQANAGEGQKLAHN
>Mature_918_residues
AKPDYYDVSECETHEKGAPKFCKKSEPGEGAERSCAYDGARVVLMPITDVIHLVHGPIACAGNSWDNRGARSSDSQLYRR
GFTTEMLENDVVFGGEKKLYRAILELAERYEGQAKAMFVYATCVTAMTGDDVEAVCAAAGKKVAIPLIPVNTPGFIGDKN
IGNRLAGEVLFKHVIGTAEPPVLGEYPINLIGEYNIAGDLWGMLPLFERLGIQVLSCFSGDATFEELRYAHRAKLNIIIC
SKSLTNLARKMQKNYGMPYLEESFYGMTDTAKALRDIARELDDAVGGLEKRIMQDRVEKLLEEEEATCRERLAPYRARLE
GKRSVLFTGGVKTWSMVNALRELGVEILAAGTQNSTLEDFYRMKALMHQDARIIEDTSSAGLLQVMYDKMPDLIVAGGKT
KFLALKTKTPFLDINHGRSHPYAGYEGMVTFAKQLDLTVNNPIWPVLNAKAPWEKTEEELTAAVALAAGHARACLDEDLK
DSTVKVPAKNATVNPQKNSPALGATLAYLGIDQMLALLHGAQGCSTFIRLQLSRHFKEPVALNSTAMSEDTAIFGGWENL
KKGLKKVIEKFSPEVVGVMTSGLTETMGDDVRSAIVHFRQEYPEHDGVPVVWASTPDYCGSLQEGYAATVEAIVRSVPEP
GETIPGQVTVLPGAHLTPADVEEVRELCEAFGLDPIIVPDIANALDGHIDETVSPLSTGGVSMARIRQAGQSAATLFIGD
SLAKAAEAMTERCGMPSYGFTSLTGLAQVDRFMETLAAIAGRPIPEKFRRWRSRLMDAMVDSHYQFGLKKVTVALEGDNL
KTLVNFLAGMGCEIQAAIAATRVRGLDGLPARDIFVGDLEDLETAARGSDLIVANSNGRQAAAKLGIKAHLRAGLPVFDR
LGAHQKMWVGYRGTMNLLFETANLFQANAGEGQKLAHN

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 100019; Mature: 99888

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPDYYDVSECETHEKGAPKFCKKSEPGEGAERSCAYDGARVVLMPITDVIHLVHGPIA
CCCCCCCCCHHHCCCCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHCCCEE
CAGNSWDNRGARSSDSQLYRRGFTTEMLENDVVFGGEKKLYRAILELAERYEGQAKAMFV
ECCCCCCCCCCCCCHHHHHHHCCCHHHHCCCCEECCHHHHHHHHHHHHHHCCCCHHEEEE
YATCVTAMTGDDVEAVCAAAGKKVAIPLIPVNTPGFIGDKNIGNRLAGEVLFKHVIGTAE
EEHHHHHHCCCCHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
PPVLGEYPINLIGEYNIAGDLWGMLPLFERLGIQVLSCFSGDATFEELRYAHRAKLNIII
CCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCEEEEEE
CSKSLTNLARKMQKNYGMPYLEESFYGMTDTAKALRDIARELDDAVGGLEKRIMQDRVEK
ECHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLEEEEATCRERLAPYRARLEGKRSVLFTGGVKTWSMVNALRELGVEILAAGTQNSTLED
HHHHHHHHHHHHHCHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCHHEEECCCCCCHHHH
FYRMKALMHQDARIIEDTSSAGLLQVMYDKMPDLIVAGGKTKFLALKTKTPFLDINHGRS
HHHHHHHHHHCHHHHHCCCCCCHHHHHHHCCCCEEEECCCEEEEEEECCCCEEECCCCCC
HPYAGYEGMVTFAKQLDLTVNNPIWPVLNAKAPWEKTEEELTAAVALAAGHARACLDEDL
CCCCCCHHHHHHHHHHCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHCCC
KDSTVKVPAKNATVNPQKNSPALGATLAYLGIDQMLALLHGAQGCSTFIRLQLSRHFKEP
CCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
VALNSTAMSEDTAIFGGWENLKKGLKKVIEKFSPEVVGVMTSGLTETMGDDVRSAIVHFR
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHH
QEYPEHDGVPVVWASTPDYCGSLQEGYAATVEAIVRSVPEPGETIPGQVTVLPGAHLTPA
HHCCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCC
DVEEVRELCEAFGLDPIIVPDIANALDGHIDETVSPLSTGGVSMARIRQAGQSAATLFIG
CHHHHHHHHHHHCCCCEEHHHHHHHHCCHHHHHHCCHHCCCHHHHHHHHCCCCCEEEEEC
DSLAKAAEAMTERCGMPSYGFTSLTGLAQVDRFMETLAAIAGRPIPEKFRRWRSRLMDAM
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VDSHYQFGLKKVTVALEGDNLKTLVNFLAGMGCEIQAAIAATRVRGLDGLPARDIFVGDL
HHHHHHCCCEEEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCEEECCH
EDLETAARGSDLIVANSNGRQAAAKLGIKAHLRAGLPVFDRLGAHQKMWVGYRGTMNLLF
HHHHHHHCCCCEEEECCCCHHHHHHHCHHHHHHCCCCHHHHCCCCCEEEEECCCHHHHHH
ETANLFQANAGEGQKLAHN
HHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AKPDYYDVSECETHEKGAPKFCKKSEPGEGAERSCAYDGARVVLMPITDVIHLVHGPIA
CCCCCCCCHHHCCCCCCCCCHHCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHCCCEE
CAGNSWDNRGARSSDSQLYRRGFTTEMLENDVVFGGEKKLYRAILELAERYEGQAKAMFV
ECCCCCCCCCCCCCHHHHHHHCCCHHHHCCCCEECCHHHHHHHHHHHHHHCCCCHHEEEE
YATCVTAMTGDDVEAVCAAAGKKVAIPLIPVNTPGFIGDKNIGNRLAGEVLFKHVIGTAE
EEHHHHHHCCCCHHHHHHHCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
PPVLGEYPINLIGEYNIAGDLWGMLPLFERLGIQVLSCFSGDATFEELRYAHRAKLNIII
CCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCEEEEEE
CSKSLTNLARKMQKNYGMPYLEESFYGMTDTAKALRDIARELDDAVGGLEKRIMQDRVEK
ECHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLEEEEATCRERLAPYRARLEGKRSVLFTGGVKTWSMVNALRELGVEILAAGTQNSTLED
HHHHHHHHHHHHHCHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCHHEEECCCCCCHHHH
FYRMKALMHQDARIIEDTSSAGLLQVMYDKMPDLIVAGGKTKFLALKTKTPFLDINHGRS
HHHHHHHHHHCHHHHHCCCCCCHHHHHHHCCCCEEEECCCEEEEEEECCCCEEECCCCCC
HPYAGYEGMVTFAKQLDLTVNNPIWPVLNAKAPWEKTEEELTAAVALAAGHARACLDEDL
CCCCCCHHHHHHHHHHCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHCCC
KDSTVKVPAKNATVNPQKNSPALGATLAYLGIDQMLALLHGAQGCSTFIRLQLSRHFKEP
CCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
VALNSTAMSEDTAIFGGWENLKKGLKKVIEKFSPEVVGVMTSGLTETMGDDVRSAIVHFR
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHH
QEYPEHDGVPVVWASTPDYCGSLQEGYAATVEAIVRSVPEPGETIPGQVTVLPGAHLTPA
HHCCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCC
DVEEVRELCEAFGLDPIIVPDIANALDGHIDETVSPLSTGGVSMARIRQAGQSAATLFIG
CHHHHHHHHHHHCCCCEEHHHHHHHHCCHHHHHHCCHHCCCHHHHHHHHCCCCCEEEEEC
DSLAKAAEAMTERCGMPSYGFTSLTGLAQVDRFMETLAAIAGRPIPEKFRRWRSRLMDAM
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
VDSHYQFGLKKVTVALEGDNLKTLVNFLAGMGCEIQAAIAATRVRGLDGLPARDIFVGDL
HHHHHHCCCEEEEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCEEECCH
EDLETAARGSDLIVANSNGRQAAAKLGIKAHLRAGLPVFDRLGAHQKMWVGYRGTMNLLF
HHHHHHHCCCCEEEECCCCHHHHHHHCHHHHHHCCCCHHHHCCCCCEEEEECCCHHHHHH
ETANLFQANAGEGQKLAHN
HHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]