Click here to switch to the map view.

The map label for this gene is pqqE [H]

Identifier: 78221808

GI number: 78221808

Start: 652645

End: 653775

Strand: Direct

Name: pqqE [H]

Synonym: Gmet_0587

Alternate gene names: 78221808

Gene position: 652645-653775 (Clockwise)

Preceding gene: 78221807

Following gene: 78221809

Centisome position: 16.33

GC content: 65.25

Gene sequence:

>1131_bases
ATGATCAGGAAGGTCTATCACGATTTCCTGCTGGCGAAGCTGAAGCAGGATGGGGTGGGGTGGCTTGCCCGGCGGGCGCG
CCAGTACCTGCTGCTGCACCTCTCGCCGCTTGCCGGGCGAGCCCTGTGCGGCCCGGTTCACGGAAGCCTCATGGTCACCC
GCCGCTGTAACTGCGACTGCACCATGTGCGACATCCCCTCGAAGCGCCGGGAATCAGCTGGCACCGGGGGCGACGAGCTG
GACACCGCACGCCTCAAGGAGATCATCGGGGAGTTTGCCCGCCTGGGAACGCCGGGCCTCGGCTTTACCGGCGGCGAGCC
GCTGCTCCGCCCGGACATCTACGAACTGCTGGCCTGCTCCCGGCACAACGGCATGATTACCCATCTCAACACCAACGGTT
ATTTTCTCGACGACACGGCGGCGCGGTGTATCGTCGATGCCGGGGTGGAATCGGTCAATATCTCGCTGGACGGGGCCTCT
CCGGCGACCCACGACCGGATCAGGAACTGCGGCGGCGCCTTCGAGCGGGCCACGGCGGCTGTGGAGCGCCTGCACCGGCT
GCGCCGGGAGCGGGGAGTGCCGCTGCGCATCAAGATGGTGGCGGTCCTCAGTGAAGCCAACATCGACGAGGCCGGGGATC
TGGCGGAACTTTCCCGCGAACTCGGCGCCGACTGCATCGAGTTCATTCCGTGCCAGCCGCTCACTGGCAATGGGGGTGAC
AAAGCGACGCCCGATGAGTCCTTCATGAAAAAAGTCGACCGGCTGGTGGAGTATCTGCTCGCAATGGGCCGGGAGCGGGG
GATGGTCGAAAATTCTCCCGCCCACCTGCGGCTGTTCCGGTCATCGGTCGCCGGTGTCCCGTCTCCGGTGCGCTGCCGGG
CAGGGTACCATTCCCTGTTGGTGGACTGCCATGGGGACGTGTTCCCCTGTTTTCCCCGGATGAGCTGGGGCCGGCGGGCC
GGCAATGTCAGGCATGGCAGCCTTGTGGATCTGTGGCGTTCCGATGGGTACCAGACCCATCGCGATATCGCGTCCGCGTG
CCGGGACTGTTATCTGAACTGTCATCTGGAACTGAATCTCTTGTTCGACCACAAAGCGCGGGGGCGATTCCGACGCGGGA
GCGAGCGGTGA

Upstream 100 bases:

>100_bases
GGCAGGCAGTTCGGGATGGGGCCTGTCCGGGATGCTGGACACCCTGCGAGGCATACCAGACCATCATGGCGAACCTGTTT
GCTGGAGGCGGCAAAGGGTG

Downstream 100 bases:

>100_bases
ATTTCTTTTCTCTGAAACGGGAACAGGCGCTGGATTTCGTCTGCCTTGCGGTGGTGGTCCTGGCTGTGTATGGGAGAGCC
CTTGGCCATGATTTCCAGAC

Product: radical SAM family protein

Products: NA

Alternate protein names: Pyrroloquinoline quinone biosynthesis protein E [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MIRKVYHDFLLAKLKQDGVGWLARRARQYLLLHLSPLAGRALCGPVHGSLMVTRRCNCDCTMCDIPSKRRESAGTGGDEL
DTARLKEIIGEFARLGTPGLGFTGGEPLLRPDIYELLACSRHNGMITHLNTNGYFLDDTAARCIVDAGVESVNISLDGAS
PATHDRIRNCGGAFERATAAVERLHRLRRERGVPLRIKMVAVLSEANIDEAGDLAELSRELGADCIEFIPCQPLTGNGGD
KATPDESFMKKVDRLVEYLLAMGRERGMVENSPAHLRLFRSSVAGVPSPVRCRAGYHSLLVDCHGDVFPCFPRMSWGRRA
GNVRHGSLVDLWRSDGYQTHRDIASACRDCYLNCHLELNLLFDHKARGRFRRGSER

Sequences:

>Translated_376_residues
MIRKVYHDFLLAKLKQDGVGWLARRARQYLLLHLSPLAGRALCGPVHGSLMVTRRCNCDCTMCDIPSKRRESAGTGGDEL
DTARLKEIIGEFARLGTPGLGFTGGEPLLRPDIYELLACSRHNGMITHLNTNGYFLDDTAARCIVDAGVESVNISLDGAS
PATHDRIRNCGGAFERATAAVERLHRLRRERGVPLRIKMVAVLSEANIDEAGDLAELSRELGADCIEFIPCQPLTGNGGD
KATPDESFMKKVDRLVEYLLAMGRERGMVENSPAHLRLFRSSVAGVPSPVRCRAGYHSLLVDCHGDVFPCFPRMSWGRRA
GNVRHGSLVDLWRSDGYQTHRDIASACRDCYLNCHLELNLLFDHKARGRFRRGSER
>Mature_376_residues
MIRKVYHDFLLAKLKQDGVGWLARRARQYLLLHLSPLAGRALCGPVHGSLMVTRRCNCDCTMCDIPSKRRESAGTGGDEL
DTARLKEIIGEFARLGTPGLGFTGGEPLLRPDIYELLACSRHNGMITHLNTNGYFLDDTAARCIVDAGVESVNISLDGAS
PATHDRIRNCGGAFERATAAVERLHRLRRERGVPLRIKMVAVLSEANIDEAGDLAELSRELGADCIEFIPCQPLTGNGGD
KATPDESFMKKVDRLVEYLLAMGRERGMVENSPAHLRLFRSSVAGVPSPVRCRAGYHSLLVDCHGDVFPCFPRMSWGRRA
GNVRHGSLVDLWRSDGYQTHRDIASACRDCYLNCHLELNLLFDHKARGRFRRGSER

Specific function: Together With Moac, Is Involved In The Conversion Of A Guanosine Derivative (Gxp) Into Molybdopterin Precursor Z. [C]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. PqqE family [H]

Homologues:

Organism=Homo sapiens, GI22027536, Length=223, Percent_Identity=30.4932735426009, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI116734668, Length=223, Percent_Identity=30.9417040358744, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786999, Length=110, Percent_Identity=37.2727272727273, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR000385
- InterPro:   IPR017200
- InterPro:   IPR011843
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 41696; Mature: 41696

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
4.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKVYHDFLLAKLKQDGVGWLARRARQYLLLHLSPLAGRALCGPVHGSLMVTRRCNCDC
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEECCCCCCHHCCCCCCCEEEEEECCCCE
TMCDIPSKRRESAGTGGDELDTARLKEIIGEFARLGTPGLGFTGGEPLLRPDIYELLACS
EEECCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
RHNGMITHLNTNGYFLDDTAARCIVDAGVESVNISLDGASPATHDRIRNCGGAFERATAA
CCCCEEEEEECCCEEECCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCCHHHHHHHH
VERLHRLRRERGVPLRIKMVAVLSEANIDEAGDLAELSRELGADCIEFIPCQPLTGNGGD
HHHHHHHHHHCCCCEEEEHEEHHHHCCCCCCCCHHHHHHHHCCHHHHHCCCCCCCCCCCC
KATPDESFMKKVDRLVEYLLAMGRERGMVENSPAHLRLFRSSVAGVPSPVRCRAGYHSLL
CCCCHHHHHHHHHHHHHHHHHHCHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHCCHHHEE
VDCHGDVFPCFPRMSWGRRAGNVRHGSLVDLWRSDGYQTHRDIASACRDCYLNCHLELNL
EECCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCEEEEEEEEEE
LFDHKARGRFRRGSER
EECCCCCCCCCCCCCC
>Mature Secondary Structure
MIRKVYHDFLLAKLKQDGVGWLARRARQYLLLHLSPLAGRALCGPVHGSLMVTRRCNCDC
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHEECCCCCCHHCCCCCCCEEEEEECCCCE
TMCDIPSKRRESAGTGGDELDTARLKEIIGEFARLGTPGLGFTGGEPLLRPDIYELLACS
EEECCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
RHNGMITHLNTNGYFLDDTAARCIVDAGVESVNISLDGASPATHDRIRNCGGAFERATAA
CCCCEEEEEECCCEEECCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCCHHHHHHHH
VERLHRLRRERGVPLRIKMVAVLSEANIDEAGDLAELSRELGADCIEFIPCQPLTGNGGD
HHHHHHHHHHCCCCEEEEHEEHHHHCCCCCCCCHHHHHHHHCCHHHHHCCCCCCCCCCCC
KATPDESFMKKVDRLVEYLLAMGRERGMVENSPAHLRLFRSSVAGVPSPVRCRAGYHSLL
CCCCHHHHHHHHHHHHHHHHHHCHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHCCHHHEE
VDCHGDVFPCFPRMSWGRRAGNVRHGSLVDLWRSDGYQTHRDIASACRDCYLNCHLELNL
EECCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHCEEEEEEEEEE
LFDHKARGRFRRGSER
EECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA