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The map label for this gene is cbiF [H]

Identifier: 78221704

GI number: 78221704

Start: 527281

End: 528057

Strand: Direct

Name: cbiF [H]

Synonym: Gmet_0483

Alternate gene names: 78221704

Gene position: 527281-528057 (Clockwise)

Preceding gene: 78221703

Following gene: 78221705

Centisome position: 13.19

GC content: 66.41

Gene sequence:

>777_bases
GTGACCATCGTCCATTTCATCGGCGCCGGTCCCGGCGATGCCGAACTTATAACTGTAAAGGGTGCCCGGCTTCTGCGGGA
GGCGGATGTGGTGGTCTATGCCGGAAGCCTCGTGGATCGGGAGCTGGTCCGCACCTACGCCCCCGATGCCGAGGTGTGCG
ATTCCGCTGCCATGACCCTGGAGGAGACCACGGCGGTCCTGGCCCAGGCGGTGGCGGACGGGAAGCGGGCCGTGCGGCTC
CACACCGGCGATCCATCCATCTACGGAGCCATTCAGGAGCAGATGGCGGAGCTGGACCGGCTCGGTATTGCCTATGCCGT
GGTGCCGGGGGTGACGAGCGCCTTTGCCGCCGCCGCCACCCTCAGGCAGGAACTGACCCTCCCTGAAGTCTCCCAGACCG
TCGTCATCACGCGCCTTGCGGGGCGGACCCCGGTGCCGGAGCGGGAGAAGCTGACCGAAATCGCCAGGATCGGGGCAACG
CTCGTCATCTACCTGTCGATCTCCATGGTGGAGCAGGTGGTGGCGGAGCTCCTGCAGGGGGCCTACACTCCGGCCACTCC
GGTAGCGGTGGTGTCCAGGGCGTCGTGGCCCGACGAGCACGTGGTGGAGGGGACCCTGGCCGACATCGCCGGAAAGGTCC
GGGACGCCGGTATCGGCAAGCAGGCCATCATCCTCGTGGGGGATGTGCTGAAGGCCCGCCGGGAAGGGCTCAAGAGCAAA
TCGCTCCTGTACGACAAGGGATTTTCCCACGAGTTCCGCAAGGGGATCGTTACCTAG

Upstream 100 bases:

>100_bases
CGGTTCCGCCGACGAAGAGGTGATCTTCGACTTGGAGCGGCTCGTGGGGGAGAAGCTCGATTACCTGTCGCTGCTGATTG
TGAAAAAGTAGGAGCCCTTA

Downstream 100 bases:

>100_bases
TTTTCTCTGTGACCTCGGTGTCCTCTGTGGCAAAGGAGGCATGTTTTGCGCATCGCCATCATCGCCATAACCCGCAACGG
CGCCCGGCTCGGCACGCAGC

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MTIVHFIGAGPGDAELITVKGARLLREADVVVYAGSLVDRELVRTYAPDAEVCDSAAMTLEETTAVLAQAVADGKRAVRL
HTGDPSIYGAIQEQMAELDRLGIAYAVVPGVTSAFAAAATLRQELTLPEVSQTVVITRLAGRTPVPEREKLTEIARIGAT
LVIYLSISMVEQVVAELLQGAYTPATPVAVVSRASWPDEHVVEGTLADIAGKVRDAGIGKQAIILVGDVLKARREGLKSK
SLLYDKGFSHEFRKGIVT

Sequences:

>Translated_258_residues
MTIVHFIGAGPGDAELITVKGARLLREADVVVYAGSLVDRELVRTYAPDAEVCDSAAMTLEETTAVLAQAVADGKRAVRL
HTGDPSIYGAIQEQMAELDRLGIAYAVVPGVTSAFAAAATLRQELTLPEVSQTVVITRLAGRTPVPEREKLTEIARIGAT
LVIYLSISMVEQVVAELLQGAYTPATPVAVVSRASWPDEHVVEGTLADIAGKVRDAGIGKQAIILVGDVLKARREGLKSK
SLLYDKGFSHEFRKGIVT
>Mature_257_residues
TIVHFIGAGPGDAELITVKGARLLREADVVVYAGSLVDRELVRTYAPDAEVCDSAAMTLEETTAVLAQAVADGKRAVRLH
TGDPSIYGAIQEQMAELDRLGIAYAVVPGVTSAFAAAATLRQELTLPEVSQTVVITRLAGRTPVPEREKLTEIARIGATL
VIYLSISMVEQVVAELLQGAYTPATPVAVVSRASWPDEHVVEGTLADIAGKVRDAGIGKQAIILVGDVLKARREGLKSKS
LLYDKGFSHEFRKGIVT

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=235, Percent_Identity=31.063829787234, Blast_Score=96, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6322922, Length=261, Percent_Identity=24.1379310344828, Blast_Score=72, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 27430; Mature: 27299

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00839 SUMT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIVHFIGAGPGDAELITVKGARLLREADVVVYAGSLVDRELVRTYAPDAEVCDSAAMTL
CEEEEEECCCCCCCEEEEECCHHHHHHCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHH
EETTAVLAQAVADGKRAVRLHTGDPSIYGAIQEQMAELDRLGIAYAVVPGVTSAFAAAAT
HHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
LRQELTLPEVSQTVVITRLAGRTPVPEREKLTEIARIGATLVIYLSISMVEQVVAELLQG
HHHHCCCCCHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AYTPATPVAVVSRASWPDEHVVEGTLADIAGKVRDAGIGKQAIILVGDVLKARREGLKSK
CCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
SLLYDKGFSHEFRKGIVT
HHHHCCCCCHHHHHCCCC
>Mature Secondary Structure 
TIVHFIGAGPGDAELITVKGARLLREADVVVYAGSLVDRELVRTYAPDAEVCDSAAMTL
EEEEEECCCCCCCEEEEECCHHHHHHCCEEEEECHHHHHHHHHHHCCCHHHHHHHHHHH
EETTAVLAQAVADGKRAVRLHTGDPSIYGAIQEQMAELDRLGIAYAVVPGVTSAFAAAAT
HHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
LRQELTLPEVSQTVVITRLAGRTPVPEREKLTEIARIGATLVIYLSISMVEQVVAELLQG
HHHHCCCCCHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AYTPATPVAVVSRASWPDEHVVEGTLADIAGKVRDAGIGKQAIILVGDVLKARREGLKSK
CCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCC
SLLYDKGFSHEFRKGIVT
HHHHCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]