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The map label for this gene is hisF

Identifier: 78221610

GI number: 78221610

Start: 432863

End: 433624

Strand: Direct

Name: hisF

Synonym: Gmet_0389

Alternate gene names: 78221610

Gene position: 432863-433624 (Clockwise)

Preceding gene: 78221609

Following gene: 78221611

Centisome position: 10.83

GC content: 65.88

Gene sequence:

>762_bases
ATGCTGACCAAGCGGATTATTCCGTGCCTCGACGTGAAGGGGGGGCGGGTCGTCAAGGGGGTCCAGTTCCTGGAGCTGCG
GGACGCCGGCGACCCGGTGGAGATCGCGGAAGCCTATGACCGCCAAGGGGCCGACGAGCTCACGTTCCTCGACATCACTG
CCTCGTCTGACGAGCGGAACATCATCATCGACGTGGTGCGCCGCACCGCCGAGCGGGTCTTCATGCCGCTCACCGTGGGG
GGCGGCGTCCGAATCGTGGACGATATCCGCAATCTCCTGAACGCCGGTGCCGACAAGGTCTCCATCAATACGGCGGCGGT
CCATCGCCCTGAGTTCGTCCGGGAGGCGGCGGAGCGCTTCGGCTCCCAGTGCACCGTGGTGGCCATCGACGCCCGGCAGG
TCCCGGGCGAAAACCGCTGGGAGGTCTACACCCATGGCGGTCGCAACCCCACCGGCATCGACGCGGTGGAGTGGGCTCGA
CGGATGGAGGAGTACGGCGCCGGCGAGATCCTCCTCACCAGCATGGACCGGGACGGCACCAAGGACGGCTACGACATCTC
TCTGACTCGCGCCATTGTCGATGCGGTCTCCATCCCGGTCATCGCCTCCGGCGGGGTGGGGAACTTGGAGCATCTCCACG
ACGGCTTCGTAAAGGCGGGAGCCTCGGCATGTCTTGCCGCCTCCATTTTTCACTACAAGGAGTACACCATCGGCGAGGCG
AAGGAGTATCTCCGTGGGCGGGGGGTCCCGGTCCGGTTATGA

Upstream 100 bases:

>100_bases
GCGAGCGGCATCGCCGGCGCCATTACGGGCAAGGCAGTCTACACCGGGGCCATCAACCTGGCCGAGGCCGTGGCCCTCAC
GAAGCGAGGAGGGGCGTAAC

Downstream 100 bases:

>100_bases
ACGAACGTGACGATATTCTGCAGGCTGTTTATCGGGTGATCAAGGAGCGCAAGGGCGCTTCCGCTGATTCTTCCTACACC
GCATCGCTCCTCCAGAAGGG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MLTKRIIPCLDVKGGRVVKGVQFLELRDAGDPVEIAEAYDRQGADELTFLDITASSDERNIIIDVVRRTAERVFMPLTVG
GGVRIVDDIRNLLNAGADKVSINTAAVHRPEFVREAAERFGSQCTVVAIDARQVPGENRWEVYTHGGRNPTGIDAVEWAR
RMEEYGAGEILLTSMDRDGTKDGYDISLTRAIVDAVSIPVIASGGVGNLEHLHDGFVKAGASACLAASIFHYKEYTIGEA
KEYLRGRGVPVRL

Sequences:

>Translated_253_residues
MLTKRIIPCLDVKGGRVVKGVQFLELRDAGDPVEIAEAYDRQGADELTFLDITASSDERNIIIDVVRRTAERVFMPLTVG
GGVRIVDDIRNLLNAGADKVSINTAAVHRPEFVREAAERFGSQCTVVAIDARQVPGENRWEVYTHGGRNPTGIDAVEWAR
RMEEYGAGEILLTSMDRDGTKDGYDISLTRAIVDAVSIPVIASGGVGNLEHLHDGFVKAGASACLAASIFHYKEYTIGEA
KEYLRGRGVPVRL
>Mature_253_residues
MLTKRIIPCLDVKGGRVVKGVQFLELRDAGDPVEIAEAYDRQGADELTFLDITASSDERNIIIDVVRRTAERVFMPLTVG
GGVRIVDDIRNLLNAGADKVSINTAAVHRPEFVREAAERFGSQCTVVAIDARQVPGENRWEVYTHGGRNPTGIDAVEWAR
RMEEYGAGEILLTSMDRDGTKDGYDISLTRAIVDAVSIPVIASGGVGNLEHLHDGFVKAGASACLAASIFHYKEYTIGEA
KEYLRGRGVPVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=42.4124513618677, Blast_Score=208, Evalue=2e-55,
Organism=Escherichia coli, GI87082028, Length=211, Percent_Identity=31.7535545023697, Blast_Score=92, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=36.3057324840764, Blast_Score=178, Evalue=6e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_GEOMG (Q39YP2)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383357.1
- ProteinModelPortal:   Q39YP2
- SMR:   Q39YP2
- STRING:   Q39YP2
- GeneID:   3739755
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0389
- NMPDR:   fig|269799.3.peg.401
- eggNOG:   COG0107
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   Q39YP2
- ProtClustDB:   PRK02083
- BioCyc:   GMET269799:GMET_0389-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 27597; Mature: 27597

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKRIIPCLDVKGGRVVKGVQFLELRDAGDPVEIAEAYDRQGADELTFLDITASSDERN
CCCCCCCCEECCCCCEEECCEEEEEEECCCCCHHHHHHHCCCCCCCEEEEEEECCCCCCH
IIIDVVRRTAERVFMPLTVGGGVRIVDDIRNLLNAGADKVSINTAAVHRPEFVREAAERF
HHHHHHHHHHHHEEEEEEECCCEEEHHHHHHHHHCCCCEEEECHHHCCCHHHHHHHHHHH
GSQCTVVAIDARQVPGENRWEVYTHGGRNPTGIDAVEWARRMEEYGAGEILLTSMDRDGT
CCCEEEEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
KDGYDISLTRAIVDAVSIPVIASGGVGNLEHLHDGFVKAGASACLAASIFHYKEYTIGEA
CCCCEEHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHH
KEYLRGRGVPVRL
HHHHHCCCCCCCC
>Mature Secondary Structure
MLTKRIIPCLDVKGGRVVKGVQFLELRDAGDPVEIAEAYDRQGADELTFLDITASSDERN
CCCCCCCCEECCCCCEEECCEEEEEEECCCCCHHHHHHHCCCCCCCEEEEEEECCCCCCH
IIIDVVRRTAERVFMPLTVGGGVRIVDDIRNLLNAGADKVSINTAAVHRPEFVREAAERF
HHHHHHHHHHHHEEEEEEECCCEEEHHHHHHHHHCCCCEEEECHHHCCCHHHHHHHHHHH
GSQCTVVAIDARQVPGENRWEVYTHGGRNPTGIDAVEWARRMEEYGAGEILLTSMDRDGT
CCCEEEEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
KDGYDISLTRAIVDAVSIPVIASGGVGNLEHLHDGFVKAGASACLAASIFHYKEYTIGEA
CCCCEEHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHH
KEYLRGRGVPVRL
HHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA