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The map label for this gene is hyfB [H]

Identifier: 78221596

GI number: 78221596

Start: 418562

End: 420571

Strand: Reverse

Name: hyfB [H]

Synonym: Gmet_0375

Alternate gene names: 78221596

Gene position: 420571-418562 (Counterclockwise)

Preceding gene: 78221640

Following gene: 78221595

Centisome position: 10.52

GC content: 64.58

Gene sequence:

>2010_bases
ATGGAAGCATCAACGGCAGGGCCTGACAATCCTGTCAGCCTGGTCATAGCCGGCGCGCTCCTGATCGGCGCGTCGGGCAT
TCCGGGCCTTTTTCTCCCCAAGAAGCTGGACTGGGGGCAGAAATGCGCCTCTCTCACCGTCGTTCTCGGCTCCATCCTGG
GAATCATCGGTGCCCTGGCAACGCTCTTTACTGAACGGACCGTCTCCTACACCCTCGTAAGCTCGCTTCCCTTCGGCCCC
CTTGAGGCCGGCATCGACCCGCTCTCCGCCTTCTTCCTCCTCCCCGTCTTCATCGTCAGCGCCTGCTGCGCCGTGTACGG
CATCGGCTACTGGCCCGCCAGCCACCACCCCCGCACGGCACCGCGGCTCACCTTTTTCTTCGGCGTCCTCGCCGCTTCCC
TGGCATTGGTCCCCCTGGCCCGGAACGGAGTCCTTTTTCTCATCTTCTGGGAGATCATGGCCCTTTCCGCCTACTTTGCC
CTCGCCACCGACGATCTGGAGCAGGAGGTTCGAGACGCATCCCTCATCTATCTCGTGGCAACCCACGCAAGCACCCTCAC
CCTCTTCGCCTGCTTCTCCCTGCTAAAAGCGACTGCCGGTTCCTTTTCATTTCCTGCGACAACGGCGCTGGACGGAACAA
CTCCCCTTGGGGCCGCCATTTTCGTGACCGCTCTCCTGGGCTTTGGCCTCAAGGCAGGGGTGATGCCGCTTCACGTCTGG
CTCCCGGGCGCCCATGCCAGCGCACCGAGCCACGTGTCGGCCCTCATGTCGGGAGTCCTCATCAAGATGGGAATCTACGG
CATGGTCCGCATCCTCTCCTTCTTCGGAGTGGTCCCGCCCTGGTGGGGGATGGTTCTTCTTATGGCGGGAATTGTGTCGG
GAATCCTCGGGGTTGCCTTTGCCGTGGGACAGCACGACCTGAAGCGGCTCCTGGCCTACCACAGCATCGAAAACATCGGC
ATCATCGTCATGGGGCTCGGCGTTGCCCTCATCGGCAGGGGAGCGGGACTGCCGCTCCTGGTGGTCCTCGGGCTCGGGGG
GGCGCTTCTCCATATCCTGAATCACGCCCTTTTCAAGTCCCTCCTCTTCCTCGGGGCCGGTGCGGTAATTCACGAGGCTG
GGACCCGGGAGATCGACAGCCTCGGCGGGCTGGGCCGACGGATGCCCTGGACCGCGGCCCTCTTTCTCACCGGCGCCGTG
GCCATTTGCGGACTCCCCCCCCTGAACGGCTTTGTGAGTGAGCTTCTCGTCTATCTCGGCTTTTTCTCCGGCCTCGTGCA
GGAAAAGGGGGCCGCGGCCCCCCTGCTGGGGCTGGCGGCGCCGGCCCTGGCCATGGTGGGAGGGATGGCGGTCCTCTGCT
TCGTGAAGGTATTCGGCACGGTCTTCCTGGGGTTACCCCGGTCGTCCGACGCGGCCGGGTGCCACGAACCGGGGTGGGCC
ATGCGGGGCCCCATGATGCTCCTTGCCATACTCTGCGCAGCCATCGGCCTCCTCCCCGTGGCACTCCCACGCTTTCTGGA
GCCGGCCCTCTTTGCCTGGAGCCCCCAGCTCCCCTTTGCCGGCGTCCGGCTGGCGGATGCGGCGCCCTTTGGCTGGATTA
CCCTCATGGGGCTCATCCTCCTTCTGGCGATTCTCGGCATCGGCGCCTGGCTCTGGAAGCGGGTAACGGATCAACCTCGG
GGGAGAGTCGGCACCTGGGGCTGCGGCTTTGCCCGTCCCACTGTCCGGATGCAGTACACCGCCTCTTCCTTTGCAGAGAT
GTTCGTGAACCTATTCGCCGCGGTGCTCCGCCCCCGGGTCCACCCGCCCAAGGTTCAGGGCCTCTTCCCGGGGAAAAGCG
CCTTTTCGAGCCATGTTCCCGAAGCGGTACTGGAACTGGTCATCACGCCGCTCCTCTCGGCCATGGACCGGAGGTTCTCT
CTGGCCCGCAAGCTCCAGCACGGCCGGCTCCACCTTTATATCCTCTACATCTTCGTGACGCTGGCGCTCTTGCTGGCGTG
GGCCTATTAA

Upstream 100 bases:

>100_bases
TAAGGACGTATAGTCCGCAAAGGTACGCACCAGTAAAATGGCGTCACACCGCGAGTGCTGCGACCACACACAGCGCTTCT
GCCTCTCCCGGAGACCATCC

Downstream 100 bases:

>100_bases
AGGAGCAGGAAGGGAATGGTCGACGCCGTTATCCATATCCTGATTACCCTGCTCATGCCGCCGCTGCTGCTCGGGGTGAT
CAACCGGACCAAGGCCATCT

Product: NADH/ubiquinone/plastoquinone (complex I)

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 669; Mature: 669

Protein sequence:

>669_residues
MEASTAGPDNPVSLVIAGALLIGASGIPGLFLPKKLDWGQKCASLTVVLGSILGIIGALATLFTERTVSYTLVSSLPFGP
LEAGIDPLSAFFLLPVFIVSACCAVYGIGYWPASHHPRTAPRLTFFFGVLAASLALVPLARNGVLFLIFWEIMALSAYFA
LATDDLEQEVRDASLIYLVATHASTLTLFACFSLLKATAGSFSFPATTALDGTTPLGAAIFVTALLGFGLKAGVMPLHVW
LPGAHASAPSHVSALMSGVLIKMGIYGMVRILSFFGVVPPWWGMVLLMAGIVSGILGVAFAVGQHDLKRLLAYHSIENIG
IIVMGLGVALIGRGAGLPLLVVLGLGGALLHILNHALFKSLLFLGAGAVIHEAGTREIDSLGGLGRRMPWTAALFLTGAV
AICGLPPLNGFVSELLVYLGFFSGLVQEKGAAAPLLGLAAPALAMVGGMAVLCFVKVFGTVFLGLPRSSDAAGCHEPGWA
MRGPMMLLAILCAAIGLLPVALPRFLEPALFAWSPQLPFAGVRLADAAPFGWITLMGLILLLAILGIGAWLWKRVTDQPR
GRVGTWGCGFARPTVRMQYTASSFAEMFVNLFAAVLRPRVHPPKVQGLFPGKSAFSSHVPEAVLELVITPLLSAMDRRFS
LARKLQHGRLHLYILYIFVTLALLLAWAY

Sequences:

>Translated_669_residues
MEASTAGPDNPVSLVIAGALLIGASGIPGLFLPKKLDWGQKCASLTVVLGSILGIIGALATLFTERTVSYTLVSSLPFGP
LEAGIDPLSAFFLLPVFIVSACCAVYGIGYWPASHHPRTAPRLTFFFGVLAASLALVPLARNGVLFLIFWEIMALSAYFA
LATDDLEQEVRDASLIYLVATHASTLTLFACFSLLKATAGSFSFPATTALDGTTPLGAAIFVTALLGFGLKAGVMPLHVW
LPGAHASAPSHVSALMSGVLIKMGIYGMVRILSFFGVVPPWWGMVLLMAGIVSGILGVAFAVGQHDLKRLLAYHSIENIG
IIVMGLGVALIGRGAGLPLLVVLGLGGALLHILNHALFKSLLFLGAGAVIHEAGTREIDSLGGLGRRMPWTAALFLTGAV
AICGLPPLNGFVSELLVYLGFFSGLVQEKGAAAPLLGLAAPALAMVGGMAVLCFVKVFGTVFLGLPRSSDAAGCHEPGWA
MRGPMMLLAILCAAIGLLPVALPRFLEPALFAWSPQLPFAGVRLADAAPFGWITLMGLILLLAILGIGAWLWKRVTDQPR
GRVGTWGCGFARPTVRMQYTASSFAEMFVNLFAAVLRPRVHPPKVQGLFPGKSAFSSHVPEAVLELVITPLLSAMDRRFS
LARKLQHGRLHLYILYIFVTLALLLAWAY
>Mature_669_residues
MEASTAGPDNPVSLVIAGALLIGASGIPGLFLPKKLDWGQKCASLTVVLGSILGIIGALATLFTERTVSYTLVSSLPFGP
LEAGIDPLSAFFLLPVFIVSACCAVYGIGYWPASHHPRTAPRLTFFFGVLAASLALVPLARNGVLFLIFWEIMALSAYFA
LATDDLEQEVRDASLIYLVATHASTLTLFACFSLLKATAGSFSFPATTALDGTTPLGAAIFVTALLGFGLKAGVMPLHVW
LPGAHASAPSHVSALMSGVLIKMGIYGMVRILSFFGVVPPWWGMVLLMAGIVSGILGVAFAVGQHDLKRLLAYHSIENIG
IIVMGLGVALIGRGAGLPLLVVLGLGGALLHILNHALFKSLLFLGAGAVIHEAGTREIDSLGGLGRRMPWTAALFLTGAV
AICGLPPLNGFVSELLVYLGFFSGLVQEKGAAAPLLGLAAPALAMVGGMAVLCFVKVFGTVFLGLPRSSDAAGCHEPGWA
MRGPMMLLAILCAAIGLLPVALPRFLEPALFAWSPQLPFAGVRLADAAPFGWITLMGLILLLAILGIGAWLWKRVTDQPR
GRVGTWGCGFARPTVRMQYTASSFAEMFVNLFAAVLRPRVHPPKVQGLFPGKSAFSSHVPEAVLELVITPLLSAMDRRFS
LARKLQHGRLHLYILYIFVTLALLLAWAY

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=186, Percent_Identity=30.1075268817204, Blast_Score=86, Evalue=9e-17,
Organism=Escherichia coli, GI1788827, Length=604, Percent_Identity=36.9205298013245, Blast_Score=328, Evalue=5e-91,
Organism=Escherichia coli, GI2367154, Length=558, Percent_Identity=35.3046594982079, Blast_Score=232, Evalue=5e-62,
Organism=Escherichia coli, GI1788831, Length=351, Percent_Identity=34.4729344729345, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1788829, Length=469, Percent_Identity=28.1449893390192, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1788613, Length=300, Percent_Identity=29.3333333333333, Blast_Score=113, Evalue=5e-26,
Organism=Escherichia coli, GI1788614, Length=534, Percent_Identity=27.3408239700375, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 70577; Mature: 70577

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEASTAGPDNPVSLVIAGALLIGASGIPGLFLPKKLDWGQKCASLTVVLGSILGIIGALA
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TLFTERTVSYTLVSSLPFGPLEAGIDPLSAFFLLPVFIVSACCAVYGIGYWPASHHPRTA
HHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PRLTFFFGVLAASLALVPLARNGVLFLIFWEIMALSAYFALATDDLEQEVRDASLIYLVA
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
THASTLTLFACFSLLKATAGSFSFPATTALDGTTPLGAAIFVTALLGFGLKAGVMPLHVW
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHCCCEEEEEE
LPGAHASAPSHVSALMSGVLIKMGIYGMVRILSFFGVVPPWWGMVLLMAGIVSGILGVAF
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
AVGQHDLKRLLAYHSIENIGIIVMGLGVALIGRGAGLPLLVVLGLGGALLHILNHALFKS
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHH
LLFLGAGAVIHEAGTREIDSLGGLGRRMPWTAALFLTGAVAICGLPPLNGFVSELLVYLG
HHHHCCCHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
FFSGLVQEKGAAAPLLGLAAPALAMVGGMAVLCFVKVFGTVFLGLPRSSDAAGCHEPGWA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
MRGPMMLLAILCAAIGLLPVALPRFLEPALFAWSPQLPFAGVRLADAAPFGWITLMGLIL
CCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCEECCCCCHHHHHHHHHHH
LLAILGIGAWLWKRVTDQPRGRVGTWGCGFARPTVRMQYTASSFAEMFVNLFAAVLRPRV
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCC
HPPKVQGLFPGKSAFSSHVPEAVLELVITPLLSAMDRRFSLARKLQHGRLHLYILYIFVT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LALLLAWAY
HHHHHHHCC
>Mature Secondary Structure
MEASTAGPDNPVSLVIAGALLIGASGIPGLFLPKKLDWGQKCASLTVVLGSILGIIGALA
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TLFTERTVSYTLVSSLPFGPLEAGIDPLSAFFLLPVFIVSACCAVYGIGYWPASHHPRTA
HHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
PRLTFFFGVLAASLALVPLARNGVLFLIFWEIMALSAYFALATDDLEQEVRDASLIYLVA
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
THASTLTLFACFSLLKATAGSFSFPATTALDGTTPLGAAIFVTALLGFGLKAGVMPLHVW
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHCCCEEEEEE
LPGAHASAPSHVSALMSGVLIKMGIYGMVRILSFFGVVPPWWGMVLLMAGIVSGILGVAF
ECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
AVGQHDLKRLLAYHSIENIGIIVMGLGVALIGRGAGLPLLVVLGLGGALLHILNHALFKS
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHHHHHH
LLFLGAGAVIHEAGTREIDSLGGLGRRMPWTAALFLTGAVAICGLPPLNGFVSELLVYLG
HHHHCCCHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
FFSGLVQEKGAAAPLLGLAAPALAMVGGMAVLCFVKVFGTVFLGLPRSSDAAGCHEPGWA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
MRGPMMLLAILCAAIGLLPVALPRFLEPALFAWSPQLPFAGVRLADAAPFGWITLMGLIL
CCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCEECCCCCHHHHHHHHHHH
LLAILGIGAWLWKRVTDQPRGRVGTWGCGFARPTVRMQYTASSFAEMFVNLFAAVLRPRV
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCC
HPPKVQGLFPGKSAFSSHVPEAVLELVITPLLSAMDRRFSLARKLQHGRLHLYILYIFVT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LALLLAWAY
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 2016588 [H]