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The map label for this gene is hyfF [H]

Identifier: 78221593

GI number: 78221593

Start: 415561

End: 416994

Strand: Reverse

Name: hyfF [H]

Synonym: Gmet_0372

Alternate gene names: 78221593

Gene position: 416994-415561 (Counterclockwise)

Preceding gene: 78221594

Following gene: 78221592

Centisome position: 10.43

GC content: 65.55

Gene sequence:

>1434_bases
ATGATTACCGGCATCCTCCTCATACCGCTTCTGGGCGCCATCACGGCCTGGCTCATCCCTTCTGACCGGAGCCGTCCCTG
GGTGCTTCCCGTCGTCGGGACGCTCCACCTGGCCCTGACGATCGAGGCGGCACTCACCCCCACATCCCCTTCGGTGGGGA
TGTGGCTCCACCTGGACGCCATCGGGAAGCTCGTACTCCTCTCCGTGAGCCTTCTCTTTTTCGCATCCGGGCTCTATGCC
GTGGAATATCTCGCCTACCGCCGGGAACGGCCGAACCGCCTCCTCTGCGCCATGCTACCGGTCTGCCTTTCGGCCATGAC
GCTCGTGGCCTTTGCCCACCACCTGGGGCTCCTCTGGGTGGCCATCGAGACGACCACCCTCACCATGACCCCCCTCCTCT
ACTTCAACCGCAACGCCCGCTCCATCGAGGCCACCTGGAAGTACATGCTCATCTGCTCCGTGGGGATCGCCCTGGCGCTC
CTGGGGCTCCTCTTCCTGGCCTACTCCACCGTGGTGGCCCGGATCGAACCGACGCTTCTCCTGGAACCCCTGGTGGCGAA
CGCGCGGCTCCTCTCCCCCGCCTGGCTCAAGGCGGCTTTCGTGTTTCTCCTCGTGGGCTACGGGACCAAGATGGGGCTCG
CGCCGCTCCACACCTGGAAGCCCGACGCCTACGGCGAAGCGCCCGGACTCGTGGGGGGGCTCCTGGCCGGAGGGCTCGTG
ACCTGCTCCTTCGTGGGGCTCCTGCGGGCCTACCAGGTCTGCGTGGCAGCCAACGGGACGACCTTCCACCGGGACGCCCT
TGTCCTCATGGGCCTCCTCTCCATGGCCACGGCCGCCGTCTTCATGGCACGCCAGGCCGACTTCAAGCGGATGCTCGCCT
ACTCCAGCGTGGAGCATGTGGGGATCCTGGCCGTGGCCCTGGGGCTCGGCAAAGGGGCTCTGGCCGGGGCACTCCTCCAT
CTGGTGAACAACAGCCTCACCAAGGGGGTCCTCTTCCTGGCCGCCGCCAACATCCACCGTTCCTACAACAGCAAACGGTG
CGAGCAGGTGCGGGGAGCACTGCGGCGTCTCCCCTGGTCGGGAGGGCTCTTCCTGGCCGGGTTCTTCGCCGTCACCGGCT
CTCCCCCCTTCGGCCTCTTCGTGAGCGAATTCTCCATCATCTCCAGCGCCATGACCCAAGGAGGAGAGTGGATCGGCGGT
CTCATCCTCTTTTTTCTGGCCGCCATTTTCCTCGGCATGGCGCGCACGGTCCTGCCGGTGGTGATGGGGGAATCGAGCCG
CATCGAACATACCGGTTACCGGGACCGCTTCCTCACGGTGGTGCCGCCGCTCATTCTTATGGGAGTCGTCCTCATGCTCG
GTATCTGGATACCGGAACCACTCAGCCGCCTCATTACGGAAGGAGCGGCCCTATTAGGAGGAAACGTGCCGTGA

Upstream 100 bases:

>100_bases
CTCATCGTCGGCATCTTCGTCATGGGGATCATCATCAACCAGATCAGCCGCGAGTTCTCGTCCATCGACACGTCGCGGCT
CACCACCCTCAGGGACTAGC

Downstream 100 bases:

>100_bases
CCCGCACCTTCTCCCTCACCTGCCTGCGCAACGGAGAGGCGGTGCCGGTGGCCTCGGTGCCGCTCCTTCCCCTCGAACAG
TTCCTCCAGGATATTATCGA

Product: NADH/ubiquinone/plastoquinone (complex I)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MITGILLIPLLGAITAWLIPSDRSRPWVLPVVGTLHLALTIEAALTPTSPSVGMWLHLDAIGKLVLLSVSLLFFASGLYA
VEYLAYRRERPNRLLCAMLPVCLSAMTLVAFAHHLGLLWVAIETTTLTMTPLLYFNRNARSIEATWKYMLICSVGIALAL
LGLLFLAYSTVVARIEPTLLLEPLVANARLLSPAWLKAAFVFLLVGYGTKMGLAPLHTWKPDAYGEAPGLVGGLLAGGLV
TCSFVGLLRAYQVCVAANGTTFHRDALVLMGLLSMATAAVFMARQADFKRMLAYSSVEHVGILAVALGLGKGALAGALLH
LVNNSLTKGVLFLAAANIHRSYNSKRCEQVRGALRRLPWSGGLFLAGFFAVTGSPPFGLFVSEFSIISSAMTQGGEWIGG
LILFFLAAIFLGMARTVLPVVMGESSRIEHTGYRDRFLTVVPPLILMGVVLMLGIWIPEPLSRLITEGAALLGGNVP

Sequences:

>Translated_477_residues
MITGILLIPLLGAITAWLIPSDRSRPWVLPVVGTLHLALTIEAALTPTSPSVGMWLHLDAIGKLVLLSVSLLFFASGLYA
VEYLAYRRERPNRLLCAMLPVCLSAMTLVAFAHHLGLLWVAIETTTLTMTPLLYFNRNARSIEATWKYMLICSVGIALAL
LGLLFLAYSTVVARIEPTLLLEPLVANARLLSPAWLKAAFVFLLVGYGTKMGLAPLHTWKPDAYGEAPGLVGGLLAGGLV
TCSFVGLLRAYQVCVAANGTTFHRDALVLMGLLSMATAAVFMARQADFKRMLAYSSVEHVGILAVALGLGKGALAGALLH
LVNNSLTKGVLFLAAANIHRSYNSKRCEQVRGALRRLPWSGGLFLAGFFAVTGSPPFGLFVSEFSIISSAMTQGGEWIGG
LILFFLAAIFLGMARTVLPVVMGESSRIEHTGYRDRFLTVVPPLILMGVVLMLGIWIPEPLSRLITEGAALLGGNVP
>Mature_477_residues
MITGILLIPLLGAITAWLIPSDRSRPWVLPVVGTLHLALTIEAALTPTSPSVGMWLHLDAIGKLVLLSVSLLFFASGLYA
VEYLAYRRERPNRLLCAMLPVCLSAMTLVAFAHHLGLLWVAIETTTLTMTPLLYFNRNARSIEATWKYMLICSVGIALAL
LGLLFLAYSTVVARIEPTLLLEPLVANARLLSPAWLKAAFVFLLVGYGTKMGLAPLHTWKPDAYGEAPGLVGGLLAGGLV
TCSFVGLLRAYQVCVAANGTTFHRDALVLMGLLSMATAAVFMARQADFKRMLAYSSVEHVGILAVALGLGKGALAGALLH
LVNNSLTKGVLFLAAANIHRSYNSKRCEQVRGALRRLPWSGGLFLAGFFAVTGSPPFGLFVSEFSIISSAMTQGGEWIGG
LILFFLAAIFLGMARTVLPVVMGESSRIEHTGYRDRFLTVVPPLILMGVVLMLGIWIPEPLSRLITEGAALLGGNVP

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Escherichia coli, GI1788831, Length=424, Percent_Identity=36.0849056603774, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1788827, Length=367, Percent_Identity=27.2479564032698, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1788613, Length=188, Percent_Identity=29.7872340425532, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1788829, Length=433, Percent_Identity=27.9445727482679, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI145693160, Length=241, Percent_Identity=27.8008298755187, Blast_Score=77, Evalue=4e-15,
Organism=Escherichia coli, GI2367154, Length=364, Percent_Identity=23.9010989010989, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 51166; Mature: 51166

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITGILLIPLLGAITAWLIPSDRSRPWVLPVVGTLHLALTIEAALTPTSPSVGMWLHLDA
CCCHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHEEEEEEEEEECCCCCCCCEEEEHHH
IGKLVLLSVSLLFFASGLYAVEYLAYRRERPNRLLCAMLPVCLSAMTLVAFAHHLGLLWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEE
AIETTTLTMTPLLYFNRNARSIEATWKYMLICSVGIALALLGLLFLAYSTVVARIEPTLL
EEEHHEEEEHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LEPLVANARLLSPAWLKAAFVFLLVGYGTKMGLAPLHTWKPDAYGEAPGLVGGLLAGGLV
HHHHHHCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
TCSFVGLLRAYQVCVAANGTTFHRDALVLMGLLSMATAAVFMARQADFKRMLAYSSVEHV
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GILAVALGLGKGALAGALLHLVNNSLTKGVLFLAAANIHRSYNSKRCEQVRGALRRLPWS
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
GGLFLAGFFAVTGSPPFGLFVSEFSIISSAMTQGGEWIGGLILFFLAAIFLGMARTVLPV
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VMGESSRIEHTGYRDRFLTVVPPLILMGVVLMLGIWIPEPLSRLITEGAALLGGNVP
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCC
>Mature Secondary Structure
MITGILLIPLLGAITAWLIPSDRSRPWVLPVVGTLHLALTIEAALTPTSPSVGMWLHLDA
CCCHHHHHHHHHHHHHHHCCCCCCCCEEEEHHHHHEEEEEEEEEECCCCCCCCEEEEHHH
IGKLVLLSVSLLFFASGLYAVEYLAYRRERPNRLLCAMLPVCLSAMTLVAFAHHLGLLWV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEE
AIETTTLTMTPLLYFNRNARSIEATWKYMLICSVGIALALLGLLFLAYSTVVARIEPTLL
EEEHHEEEEHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LEPLVANARLLSPAWLKAAFVFLLVGYGTKMGLAPLHTWKPDAYGEAPGLVGGLLAGGLV
HHHHHHCCHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
TCSFVGLLRAYQVCVAANGTTFHRDALVLMGLLSMATAAVFMARQADFKRMLAYSSVEHV
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GILAVALGLGKGALAGALLHLVNNSLTKGVLFLAAANIHRSYNSKRCEQVRGALRRLPWS
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCC
GGLFLAGFFAVTGSPPFGLFVSEFSIISSAMTQGGEWIGGLILFFLAAIFLGMARTVLPV
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VMGESSRIEHTGYRDRFLTVVPPLILMGVVLMLGIWIPEPLSRLITEGAALLGGNVP
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]