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The map label for this gene is oprF [H]

Identifier: 78221563

GI number: 78221563

Start: 380788

End: 382131

Strand: Direct

Name: oprF [H]

Synonym: Gmet_0342

Alternate gene names: 78221563

Gene position: 380788-382131 (Clockwise)

Preceding gene: 78221561

Following gene: 78221567

Centisome position: 9.53

GC content: 60.71

Gene sequence:

>1344_bases
ATGAAGAAGCAAGTAGCAATTACCGTTGCCGCGGCGTGTCTGGCATTCTCCGCTACCGCCCACAGTGAGAGCCGCATCAA
GGAAGGTGCTTTGTCGGTGAGCCCCTTCGGTGGGTACACGTTCTTCGACCGGGACTCTGATCTCAAGAGCCGGCCCACCG
GCGGTCTCAGGCTCGGCTACAACTTCACCGAGAACGTGGCAGTCGAGGGTTCGGGAGCCTATACCGACACCAAGTACGAT
AACGGCAATGGAAGCTCCAATGTCTATCGTTATGGCGCCGAGCTCCTCTATCACTTTATTCCCCAGTCACGGGTTGTGCC
GTTCCTGGCAGCGGGGCTCGGCGGTATTACCTACGAAGGCCGTGAAGGTGTGCCCGATGCAGATCGGACCGACGGGATAC
TCACTGCCGGCGGCGGTGTGAAGCTCTTCCTTGTGGATCGTATTGCGCTTCGCGCCGATGGCCGCAACATTTTTACCTTC
AACGATGCTCTGAATCATCAGGAAGTCACCGCCGGCCTCGATTTTCAGTTCGGCGGCGGCAAGGCTGCTCCGGCTCCGGT
CAAGGCTGAACCGAAGCCCGAGCCCAAGCCCGAGCCCAAAAAGGTTGTCGCTACTGCACCCCTCGACAGTGACGGCGATG
GCGTACCCGACAATCTGGACAAGTGCCCCGATACCCCGGCCGGCGTCAAGGTCGACAGCGTCGGCTGTCCGCTGGACAGC
GACGGTGACGGCGTACCCGATTACCTGGACAAGTGCCCCGGCACCCCGGCCGGCGTGAAGGTCGACAGCGTCGGCTGTCC
GCTGGACAGCGACGGTGACGGCGTACCCGATTACCTGGACAAGTGTCCCGGCACCCCGGCCGGCGTGAAGGTCGACAGCG
TCGGCTGCCCACTGGATACGGATGGTGACGGCGTACCCGACTACCTGGACAAGTGCCCCGATACGCCGAAAAATGCCGCC
GTCGACAAGAACGGCTGCCCGGAGAAGGTCTGCATCACCCTGAAGGTTGAGTTCGACTTCGATAAGGCCAACATCAAGCC
CGAGTTCCGCGATGAGATCAAGAGAGTGGCCGACTTCCTCAAAGAGTATCCCGACGCCACTGCGGTGATCGAAGGGCATA
CCGACAGCAAAGGAACTGTGAAGTACAACCAGAAGCTCTCCGAGCGCAGGGCCAAGGCCGTCATGAAGAACCTGGTCGAC
GAATTCGGAATCTCCGCCAAGCGTCTTTCTGCCAAGGGGTATGGCGAGACCAAGCCGGTTGCCGACAACGCCACCGAAGA
AGGGCGCCAGAAGAACCGCCGCGTAGTGGCCAACATCGACTGCGGGCTGAAGAAGCGCAAGTAA

Upstream 100 bases:

>100_bases
ATCATGTCGTTTGAAATAATTAAATGATCTCTGTTTGCTTGCCTTTAGGTAAAAGGTGTTGCATCCACGAGGTATGAATT
CAACGTCAAGGAGGTATGGA

Downstream 100 bases:

>100_bases
CCGTAACAGTACAACTGACGTCATCAAAAAAACGCACTGCTTTCGGGCAGTGCGTTTTTTTGTGGCCTGTGATCCTGAAC
GATACCTGGATGGGCTACAA

Product: thrombospondin type 3 repeat-containing OmpA/MotB protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MKKQVAITVAAACLAFSATAHSESRIKEGALSVSPFGGYTFFDRDSDLKSRPTGGLRLGYNFTENVAVEGSGAYTDTKYD
NGNGSSNVYRYGAELLYHFIPQSRVVPFLAAGLGGITYEGREGVPDADRTDGILTAGGGVKLFLVDRIALRADGRNIFTF
NDALNHQEVTAGLDFQFGGGKAAPAPVKAEPKPEPKPEPKKVVATAPLDSDGDGVPDNLDKCPDTPAGVKVDSVGCPLDS
DGDGVPDYLDKCPGTPAGVKVDSVGCPLDSDGDGVPDYLDKCPGTPAGVKVDSVGCPLDTDGDGVPDYLDKCPDTPKNAA
VDKNGCPEKVCITLKVEFDFDKANIKPEFRDEIKRVADFLKEYPDATAVIEGHTDSKGTVKYNQKLSERRAKAVMKNLVD
EFGISAKRLSAKGYGETKPVADNATEEGRQKNRRVVANIDCGLKKRK

Sequences:

>Translated_447_residues
MKKQVAITVAAACLAFSATAHSESRIKEGALSVSPFGGYTFFDRDSDLKSRPTGGLRLGYNFTENVAVEGSGAYTDTKYD
NGNGSSNVYRYGAELLYHFIPQSRVVPFLAAGLGGITYEGREGVPDADRTDGILTAGGGVKLFLVDRIALRADGRNIFTF
NDALNHQEVTAGLDFQFGGGKAAPAPVKAEPKPEPKPEPKKVVATAPLDSDGDGVPDNLDKCPDTPAGVKVDSVGCPLDS
DGDGVPDYLDKCPGTPAGVKVDSVGCPLDSDGDGVPDYLDKCPGTPAGVKVDSVGCPLDTDGDGVPDYLDKCPDTPKNAA
VDKNGCPEKVCITLKVEFDFDKANIKPEFRDEIKRVADFLKEYPDATAVIEGHTDSKGTVKYNQKLSERRAKAVMKNLVD
EFGISAKRLSAKGYGETKPVADNATEEGRQKNRRVVANIDCGLKKRK
>Mature_447_residues
MKKQVAITVAAACLAFSATAHSESRIKEGALSVSPFGGYTFFDRDSDLKSRPTGGLRLGYNFTENVAVEGSGAYTDTKYD
NGNGSSNVYRYGAELLYHFIPQSRVVPFLAAGLGGITYEGREGVPDADRTDGILTAGGGVKLFLVDRIALRADGRNIFTF
NDALNHQEVTAGLDFQFGGGKAAPAPVKAEPKPEPKPEPKKVVATAPLDSDGDGVPDNLDKCPDTPAGVKVDSVGCPLDS
DGDGVPDYLDKCPGTPAGVKVDSVGCPLDSDGDGVPDYLDKCPGTPAGVKVDSVGCPLDTDGDGVPDYLDKCPDTPKNAA
VDKNGCPEKVCITLKVEFDFDKANIKPEFRDEIKRVADFLKEYPDATAVIEGHTDSKGTVKYNQKLSERRAKAVMKNLVD
EFGISAKRLSAKGYGETKPVADNATEEGRQKNRRVVANIDCGLKKRK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=100, Percent_Identity=40, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1786962, Length=101, Percent_Identity=40.5940594059406, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787191, Length=114, Percent_Identity=39.4736842105263, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 47522; Mature: 47522

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS01068 OMPA_1 ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQVAITVAAACLAFSATAHSESRIKEGALSVSPFGGYTFFDRDSDLKSRPTGGLRLGY
CCCEEEHHHHHHHHHHHHCCCCHHHHHHCCEEECCCCCEEEECCCCCCCCCCCCCEEECC
NFTENVAVEGSGAYTDTKYDNGNGSSNVYRYGAELLYHFIPQSRVVPFLAAGLGGITYEG
CCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECC
REGVPDADRTDGILTAGGGVKLFLVDRIALRADGRNIFTFNDALNHQEVTAGLDFQFGGG
CCCCCCCCCCCCEEECCCCEEEEEEEEEEEEECCCEEEEECCCCCCCCEEECCEEEECCC
KAAPAPVKAEPKPEPKPEPKKVVATAPLDSDGDGVPDNLDKCPDTPAGVKVDSVGCPLDS
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCCCCEEECCCCCCCCC
DGDGVPDYLDKCPGTPAGVKVDSVGCPLDSDGDGVPDYLDKCPGTPAGVKVDSVGCPLDT
CCCCCCHHHHHCCCCCCCEEECCCCCCCCCCCCCCCHHHHHCCCCCCCEEECCCCCCCCC
DGDGVPDYLDKCPDTPKNAAVDKNGCPEKVCITLKVEFDFDKANIKPEFRDEIKRVADFL
CCCCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHH
KEYPDATAVIEGHTDSKGTVKYNQKLSERRAKAVMKNLVDEFGISAKRLSAKGYGETKPV
HHCCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCC
ADNATEEGRQKNRRVVANIDCGLKKRK
CCCCHHHHHHHCCEEEEEECCCCCCCC
>Mature Secondary Structure
MKKQVAITVAAACLAFSATAHSESRIKEGALSVSPFGGYTFFDRDSDLKSRPTGGLRLGY
CCCEEEHHHHHHHHHHHHCCCCHHHHHHCCEEECCCCCEEEECCCCCCCCCCCCCEEECC
NFTENVAVEGSGAYTDTKYDNGNGSSNVYRYGAELLYHFIPQSRVVPFLAAGLGGITYEG
CCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEECC
REGVPDADRTDGILTAGGGVKLFLVDRIALRADGRNIFTFNDALNHQEVTAGLDFQFGGG
CCCCCCCCCCCCEEECCCCEEEEEEEEEEEEECCCEEEEECCCCCCCCEEECCEEEECCC
KAAPAPVKAEPKPEPKPEPKKVVATAPLDSDGDGVPDNLDKCPDTPAGVKVDSVGCPLDS
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCCCCEEECCCCCCCCC
DGDGVPDYLDKCPGTPAGVKVDSVGCPLDSDGDGVPDYLDKCPGTPAGVKVDSVGCPLDT
CCCCCCHHHHHCCCCCCCEEECCCCCCCCCCCCCCCHHHHHCCCCCCCEEECCCCCCCCC
DGDGVPDYLDKCPDTPKNAAVDKNGCPEKVCITLKVEFDFDKANIKPEFRDEIKRVADFL
CCCCCCHHHHHCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHH
KEYPDATAVIEGHTDSKGTVKYNQKLSERRAKAVMKNLVDEFGISAKRLSAKGYGETKPV
HHCCCCEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCC
ADNATEEGRQKNRRVVANIDCGLKKRK
CCCCHHHHHHHCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2447060; 10984043 [H]