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The map label for this gene is fixL [H]

Identifier: 78221521

GI number: 78221521

Start: 334861

End: 335310

Strand: Direct

Name: fixL [H]

Synonym: Gmet_0298

Alternate gene names: 78221521

Gene position: 334861-335310 (Clockwise)

Preceding gene: 78221520

Following gene: 78221522

Centisome position: 8.38

GC content: 66.67

Gene sequence:

>450_bases
ATGCCAGACACTACGAACGACAAGCTCTTGCGCCTCATCGCTGAAGGAGTCCCCGACGCCGTCATCTTCGCCGACCGGGA
AGGAATTGTCCGGATCTGGAGCCCCGGGGCCGAGCGGATGTTCGGCTTCACCGCCGCCGAGGCGGTTGGGCAGTCCCTGG
ACCTCATCATCCCCGAGAACCTCCGGGCCCGGCACTGGGAAGGGTACCGCAGGGTCATGGAAACAGGAGAAACCCACTAC
GGGACAAAGCTCCTGTCGGCACCGGCCCTGTGCAGCGCCGGCGGCCGCATCTCCACCGAGTTCTCCATGGCGCTCGTGCG
CGACTGCGAGGGAGCCATGGCCGGCAGCGGCGCCATCATCCGCGACGTGACCGAGCGCTGGCAGAAGGAAAAGGCCATGA
AGGAGCGGTTGGCGGAGCTGGAGCGGCACGAGGCGGAGAGGAACGGGTAG

Upstream 100 bases:

>100_bases
CTGAGCCATTGAGGCTGCAATAAATTCGGCACAGCTTGACGCAGGTCAAGGAACAGGTACCGTATTATCTCGTAAGGTAG
CAAAAACAGGAGGTGGTCCC

Downstream 100 bases:

>100_bases
GTGGAATACTTCATTGCAGCACATGTCCTCTGCTGGGGGCTCGTGGCGGTCGTCGCTCTTTTCCGACCGCTATACGGCGA
AAGCGACCGATGAAGCGTCG

Product: putative PAS/PAC sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 148

Protein sequence:

>149_residues
MPDTTNDKLLRLIAEGVPDAVIFADREGIVRIWSPGAERMFGFTAAEAVGQSLDLIIPENLRARHWEGYRRVMETGETHY
GTKLLSAPALCSAGGRISTEFSMALVRDCEGAMAGSGAIIRDVTERWQKEKAMKERLAELERHEAERNG

Sequences:

>Translated_149_residues
MPDTTNDKLLRLIAEGVPDAVIFADREGIVRIWSPGAERMFGFTAAEAVGQSLDLIIPENLRARHWEGYRRVMETGETHY
GTKLLSAPALCSAGGRISTEFSMALVRDCEGAMAGSGAIIRDVTERWQKEKAMKERLAELERHEAERNG
>Mature_148_residues
PDTTNDKLLRLIAEGVPDAVIFADREGIVRIWSPGAERMFGFTAAEAVGQSLDLIIPENLRARHWEGYRRVMETGETHYG
TKLLSAPALCSAGGRISTEFSMALVRDCEGAMAGSGAIIRDVTERWQKEKAMKERLAELERHEAERNG

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG2202

COG function: function code T; FOG: PAS/PAC domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 16532; Mature: 16401

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS50112 PAS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDTTNDKLLRLIAEGVPDAVIFADREGIVRIWSPGAERMFGFTAAEAVGQSLDLIIPEN
CCCCCHHHHHHHHHHCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHCCCCCEECCCC
LRARHWEGYRRVMETGETHYGTKLLSAPALCSAGGRISTEFSMALVRDCEGAMAGSGAII
CCHHHHHHHHHHHHCCCCCCCHHHHCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHH
RDVTERWQKEKAMKERLAELERHEAERNG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PDTTNDKLLRLIAEGVPDAVIFADREGIVRIWSPGAERMFGFTAAEAVGQSLDLIIPEN
CCCCHHHHHHHHHHCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHHCCCCCEECCCC
LRARHWEGYRRVMETGETHYGTKLLSAPALCSAGGRISTEFSMALVRDCEGAMAGSGAII
CCHHHHHHHHHHHHCCCCCCCHHHHCCCCHHCCCCCCCHHHHHHHHHHHCCCCCCCCHHH
RDVTERWQKEKAMKERLAELERHEAERNG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2000090; 12597275; 9860942; 10747783 [H]