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The map label for this gene is nrfA [H]

Identifier: 78221519

GI number: 78221519

Start: 332235

End: 333692

Strand: Direct

Name: nrfA [H]

Synonym: Gmet_0296

Alternate gene names: 78221519

Gene position: 332235-333692 (Clockwise)

Preceding gene: 78221515

Following gene: 78221520

Centisome position: 8.31

GC content: 61.8

Gene sequence:

>1458_bases
ATGCTGATGAGTAATGTGAAGCGGTGGGCCGCCGTCACGGCTTTTACCGCAATGGCGGCGGTCGGTACGGGGTGTGCCCC
GAAAAAGGTGGAACAGGACAAATCGGCGAGCGTCGCCGACGGGACCATCGATCCGGCCGGATGGAAAAAGGCCTATCCGG
CCCAGTACGAGCTCTGGAAGAAGACCGAGGAGCCGACCCCGACGGGCAAAAGCCGTTACAAGAAGGGGTATGACGAGGAC
GGGGTTTTCTACGACAAGCTGGATGAGTACCCCTTCCTGGCGCTCCTTTACAACGGCTGGGGGTTCGGCGTCGAGTACAA
GGAGCCCCGGGGTCACGCCTACATGATCCATGACCAGTTCGAGGTGGACCCGGCACGGCTCAAGGCTGGCGGCTCCTGCC
TCACCTGCAAGACCCCCTATGCGCCGGAGCTGCAGCAGCGGCTCGGCAAGGACTACTTCTCGAAGCCCTATAAGGAAGTT
CACGCCCAGATTCCCAAGGAACACCAGATGCTCGGTGTGACCTGCATCGACTGTCACAACGGCAGTGACCTGTCGCTGAA
GATCTCCCGCGGCTTCACCCTCGGCAAGGCCCTGGAGAAGCTCGGCGTCGACCGGGCCAAGCTCTCGGCCCAGGACAAGC
GGACCCTTGTCTGTGCCCAGTGCCACGTCACCTACGCCATCCCCAAGGATGGGAACATGAAATCCACGGACGTCTACTTC
CCCTGGGACGGCAGCACCTACGGCGGCATCACCATCGAGAATATCATCAAAAAGCTCCGGAGCAGTCCGGCCAACGGCGA
GTGGACCCAGGGAGTGACCGGCTTCAAACTGGCCTTTATCCGCCACCCCGAGTTCGAGCTTTTCTCCAACAACAGCCCCC
ACTGGCAGGCGGGTGTCTCCTGCGCCGACTGCCACATGCCTTCTACTACCGCGGGGAATCAGAAGGTTTCCGACCACCGG
GTCATGAGCCCCCTGAAGAACGACCTCAAGGCCTGCGCCCAGTGCCACACCGAGACCCCGGACCAGCTCCGCGGCAAGGT
CTACGCCATCCAGGACCGCACCATGTCCCGGTTTATTCGGACGGGCTATGCCACCGCCACCGTGGCCAAGCTCTTCGAGA
TGACCAATAAGGCCGAGGCCCAGGGGAAACAGATCGATAAGACCCTCTACGCCCAGGCCAAGGACCACTACGAAGAGGCC
TTCTACCGGGTGGTCTTTATCGGCGCCGAGAACTCGGTTGGCTTCCACAACCCGACCGAGGCCACGCGGGTGCTGGAAGA
TGCGGCCATGCACGCTGGCAAGGCCGACGGGCTCCTGCGGCAGGCCCTGGCCAAGGCAGGGGTGACGGTTCCCGCAAAGG
TCGACCTGGAACTGGAGAAGTACGTGAACAATCGCGGAAGCAAGAAGTTGATGTTCAAGCCCGAGCACGAGATCAAAGAC
CCCCTTGCGGGGAAGTAA

Upstream 100 bases:

>100_bases
CTGCTCCCGGCAGCTGCGGTACATTTTGTCGGCACATGATGAAAGGGGCAGGACCAGAGGATGCAAATCTCCCACGAAAG
GAGCAAGAGAGGTGAATGAA

Downstream 100 bases:

>100_bases
AGAATATGGGAATGGGAACCGGAGCCGGTAGAGCAATCTGCCGGCTCCGGCCTTTTTCCGAACGAGGTACCAATGAACAA
ACCGAATTTCCACTCACGCA

Product: respiratory nitrite reductase (cytochrome; ammonia-forming) precursor

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MLMSNVKRWAAVTAFTAMAAVGTGCAPKKVEQDKSASVADGTIDPAGWKKAYPAQYELWKKTEEPTPTGKSRYKKGYDED
GVFYDKLDEYPFLALLYNGWGFGVEYKEPRGHAYMIHDQFEVDPARLKAGGSCLTCKTPYAPELQQRLGKDYFSKPYKEV
HAQIPKEHQMLGVTCIDCHNGSDLSLKISRGFTLGKALEKLGVDRAKLSAQDKRTLVCAQCHVTYAIPKDGNMKSTDVYF
PWDGSTYGGITIENIIKKLRSSPANGEWTQGVTGFKLAFIRHPEFELFSNNSPHWQAGVSCADCHMPSTTAGNQKVSDHR
VMSPLKNDLKACAQCHTETPDQLRGKVYAIQDRTMSRFIRTGYATATVAKLFEMTNKAEAQGKQIDKTLYAQAKDHYEEA
FYRVVFIGAENSVGFHNPTEATRVLEDAAMHAGKADGLLRQALAKAGVTVPAKVDLELEKYVNNRGSKKLMFKPEHEIKD
PLAGK

Sequences:

>Translated_485_residues
MLMSNVKRWAAVTAFTAMAAVGTGCAPKKVEQDKSASVADGTIDPAGWKKAYPAQYELWKKTEEPTPTGKSRYKKGYDED
GVFYDKLDEYPFLALLYNGWGFGVEYKEPRGHAYMIHDQFEVDPARLKAGGSCLTCKTPYAPELQQRLGKDYFSKPYKEV
HAQIPKEHQMLGVTCIDCHNGSDLSLKISRGFTLGKALEKLGVDRAKLSAQDKRTLVCAQCHVTYAIPKDGNMKSTDVYF
PWDGSTYGGITIENIIKKLRSSPANGEWTQGVTGFKLAFIRHPEFELFSNNSPHWQAGVSCADCHMPSTTAGNQKVSDHR
VMSPLKNDLKACAQCHTETPDQLRGKVYAIQDRTMSRFIRTGYATATVAKLFEMTNKAEAQGKQIDKTLYAQAKDHYEEA
FYRVVFIGAENSVGFHNPTEATRVLEDAAMHAGKADGLLRQALAKAGVTVPAKVDLELEKYVNNRGSKKLMFKPEHEIKD
PLAGK
>Mature_485_residues
MLMSNVKRWAAVTAFTAMAAVGTGCAPKKVEQDKSASVADGTIDPAGWKKAYPAQYELWKKTEEPTPTGKSRYKKGYDED
GVFYDKLDEYPFLALLYNGWGFGVEYKEPRGHAYMIHDQFEVDPARLKAGGSCLTCKTPYAPELQQRLGKDYFSKPYKEV
HAQIPKEHQMLGVTCIDCHNGSDLSLKISRGFTLGKALEKLGVDRAKLSAQDKRTLVCAQCHVTYAIPKDGNMKSTDVYF
PWDGSTYGGITIENIIKKLRSSPANGEWTQGVTGFKLAFIRHPEFELFSNNSPHWQAGVSCADCHMPSTTAGNQKVSDHR
VMSPLKNDLKACAQCHTETPDQLRGKVYAIQDRTMSRFIRTGYATATVAKLFEMTNKAEAQGKQIDKTLYAQAKDHYEEA
FYRVVFIGAENSVGFHNPTEATRVLEDAAMHAGKADGLLRQALAKAGVTVPAKVDLELEKYVNNRGSKKLMFKPEHEIKD
PLAGK

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=418, Percent_Identity=27.9904306220096, Blast_Score=141, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 53892; Mature: 53892

Theoretical pI: Translated: 8.76; Mature: 8.76

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMSNVKRWAAVTAFTAMAAVGTGCAPKKVEQDKSASVADGTIDPAGWKKAYPAQYELWK
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
KTEEPTPTGKSRYKKGYDEDGVFYDKLDEYPFLALLYNGWGFGVEYKEPRGHAYMIHDQF
CCCCCCCCCHHHHHCCCCCCCCCHHHHCCCCEEEEEECCCCCCCEEECCCCCEEEEECCC
EVDPARLKAGGSCLTCKTPYAPELQQRLGKDYFSKPYKEVHAQIPKEHQMLGVTCIDCHN
CCCHHHHCCCCCEEEECCCCCHHHHHHHCHHHHHCCHHHHHHHCCCCCCEEEEEEEECCC
GSDLSLKISRGFTLGKALEKLGVDRAKLSAQDKRTLVCAQCHVTYAIPKDGNMKSTDVYF
CCCEEEEEECCCHHHHHHHHHCCCHHHHCCCCCCEEEEEEEEEEEEECCCCCCCCCEEEE
PWDGSTYGGITIENIIKKLRSSPANGEWTQGVTGFKLAFIRHPEFELFSNNSPHWQAGVS
CCCCCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCCHHHCCC
CADCHMPSTTAGNQKVSDHRVMSPLKNDLKACAQCHTETPDQLRGKVYAIQDRTMSRFIR
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECHHHHHHHHH
TGYATATVAKLFEMTNKAEAQGKQIDKTLYAQAKDHYEEAFYRVVFIGAENSVGFHNPTE
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCHH
ATRVLEDAAMHAGKADGLLRQALAKAGVTVPAKVDLELEKYVNNRGSKKLMFKPEHEIKD
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEECHHHHHHHCCCCCEEEEECCCHHHCC
PLAGK
CCCCC
>Mature Secondary Structure
MLMSNVKRWAAVTAFTAMAAVGTGCAPKKVEQDKSASVADGTIDPAGWKKAYPAQYELWK
CCCHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
KTEEPTPTGKSRYKKGYDEDGVFYDKLDEYPFLALLYNGWGFGVEYKEPRGHAYMIHDQF
CCCCCCCCCHHHHHCCCCCCCCCHHHHCCCCEEEEEECCCCCCCEEECCCCCEEEEECCC
EVDPARLKAGGSCLTCKTPYAPELQQRLGKDYFSKPYKEVHAQIPKEHQMLGVTCIDCHN
CCCHHHHCCCCCEEEECCCCCHHHHHHHCHHHHHCCHHHHHHHCCCCCCEEEEEEEECCC
GSDLSLKISRGFTLGKALEKLGVDRAKLSAQDKRTLVCAQCHVTYAIPKDGNMKSTDVYF
CCCEEEEEECCCHHHHHHHHHCCCHHHHCCCCCCEEEEEEEEEEEEECCCCCCCCCEEEE
PWDGSTYGGITIENIIKKLRSSPANGEWTQGVTGFKLAFIRHPEFELFSNNSPHWQAGVS
CCCCCCCCCEEHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCEEECCCCCCHHHCCC
CADCHMPSTTAGNQKVSDHRVMSPLKNDLKACAQCHTETPDQLRGKVYAIQDRTMSRFIR
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECHHHHHHHHH
TGYATATVAKLFEMTNKAEAQGKQIDKTLYAQAKDHYEEAFYRVVFIGAENSVGFHNPTE
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCHH
ATRVLEDAAMHAGKADGLLRQALAKAGVTVPAKVDLELEKYVNNRGSKKLMFKPEHEIKD
HHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEECHHHHHHHCCCCCEEEEECCCHHHCC
PLAGK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA