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The map label for this gene is dusB [H]

Identifier: 78221481

GI number: 78221481

Start: 289629

End: 290624

Strand: Reverse

Name: dusB [H]

Synonym: Gmet_0258

Alternate gene names: 78221481

Gene position: 290624-289629 (Counterclockwise)

Preceding gene: 78221482

Following gene: 78221477

Centisome position: 7.27

GC content: 66.27

Gene sequence:

>996_bases
ATGCACGACACAACTCCATCACCCGTTACAACTCCACGCACTCCCCTCCCCTGGCCGACCGGCCACACCCCCCTGATGCT
GGCCCCCATGCAGGGGCTGACGAACCGCGCCCTGCGTGCCCTGTTCATCAACTGGGTACGGCCCGATGTGGTCTTTACCG
AGTTCATGCGGGTCAATCCCGGCGGAGCGCGGCAGCCGATCAGGGCGGGGGATCTGCGGGAGGTGGCCGCCGTGGAGGGG
GGCGTCCCCCTGGTGGTACAGCTCATCGGTCACGACCATAAAGCCCTGGTGGCCGCTGCCCGGACGGCTCAGGAGGCCGG
GGCAGTCCACATCAACCTTAATCTCGGCTGTCCCTACGGACGGATGACTTCCGGCCTCACCGGCGGAGGGATGCTGCACC
GCCCCGAGGCCCTGGAAGAGCTCATCCCAGCCCTGCGGAGGGCCATTGCGGGGACATTTTCCGTCAAGGTCAGGGCAGGC
TACGACGACTCCGGGCAGATAATGACCCTCCTCCCCCTGTTCGAGGAGGCGGGGGTCGACTTCCTGGTGCTCCATCCCCG
GACGGTTGTGCAGGAATACACCGGCCACGCCGACCATGCCGTTACCGCCGAGGTGGTGCGGCAGACCCGGCTCCCGGTCA
TCGCCAACGGCGACGTCCGGAGCGCCGCCGACGGTCTGCGGGTCCTGCGGAAAACGGGGGCGACGGGACTCATGCTGGGG
AGGGGAGCCATCGCCGACCCCCTGCTGTTCCAGCGGCTCAGGGGCAAGGCGCCGGCCGAACCGACCGGTGAGGAACGGGT
GGCAATGCTCAAGCGTTATCTGGGGGAGTTGCTGGAGGGATACGGCGAAATCTTCTGCGGCGACACGCAGATTCTGGGGA
AGATCAAGGGAGTCATGGCCACCATGGACGATCCCTTCCTGGCAAAGGGGCTCAAACAGCTCAAGAAAGCGCGGACTGTC
GCGGCCTTCGCCTCCACCCTGAGAGACATCCAATGA

Upstream 100 bases:

>100_bases
GAGCTGGTGGAGATCCTCTAGGATTTTCCCGAAAAATCGGCGTACGAGAACAGCAAAGGCGGCCCATGGCCGCCTTTGTC
GTCTTTCCCCCTTAGCCACT

Downstream 100 bases:

>100_bases
GAAAAGGAGCAGTTTCCCGCCCCCTTTGGCACAATGTCACATTATACCGGGAAAAAATTTTTTTTACAGTCTGTGCAGGG
AATTTTTCCAAAGGTATAGT

Product: dihydrouridine synthase, DuS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MHDTTPSPVTTPRTPLPWPTGHTPLMLAPMQGLTNRALRALFINWVRPDVVFTEFMRVNPGGARQPIRAGDLREVAAVEG
GVPLVVQLIGHDHKALVAAARTAQEAGAVHINLNLGCPYGRMTSGLTGGGMLHRPEALEELIPALRRAIAGTFSVKVRAG
YDDSGQIMTLLPLFEEAGVDFLVLHPRTVVQEYTGHADHAVTAEVVRQTRLPVIANGDVRSAADGLRVLRKTGATGLMLG
RGAIADPLLFQRLRGKAPAEPTGEERVAMLKRYLGELLEGYGEIFCGDTQILGKIKGVMATMDDPFLAKGLKQLKKARTV
AAFASTLRDIQ

Sequences:

>Translated_331_residues
MHDTTPSPVTTPRTPLPWPTGHTPLMLAPMQGLTNRALRALFINWVRPDVVFTEFMRVNPGGARQPIRAGDLREVAAVEG
GVPLVVQLIGHDHKALVAAARTAQEAGAVHINLNLGCPYGRMTSGLTGGGMLHRPEALEELIPALRRAIAGTFSVKVRAG
YDDSGQIMTLLPLFEEAGVDFLVLHPRTVVQEYTGHADHAVTAEVVRQTRLPVIANGDVRSAADGLRVLRKTGATGLMLG
RGAIADPLLFQRLRGKAPAEPTGEERVAMLKRYLGELLEGYGEIFCGDTQILGKIKGVMATMDDPFLAKGLKQLKKARTV
AAFASTLRDIQ
>Mature_331_residues
MHDTTPSPVTTPRTPLPWPTGHTPLMLAPMQGLTNRALRALFINWVRPDVVFTEFMRVNPGGARQPIRAGDLREVAAVEG
GVPLVVQLIGHDHKALVAAARTAQEAGAVHINLNLGCPYGRMTSGLTGGGMLHRPEALEELIPALRRAIAGTFSVKVRAG
YDDSGQIMTLLPLFEEAGVDFLVLHPRTVVQEYTGHADHAVTAEVVRQTRLPVIANGDVRSAADGLRVLRKTGATGLMLG
RGAIADPLLFQRLRGKAPAEPTGEERVAMLKRYLGELLEGYGEIFCGDTQILGKIKGVMATMDDPFLAKGLKQLKKARTV
AAFASTLRDIQ

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=235, Percent_Identity=28.0851063829787, Blast_Score=95, Evalue=9e-20,
Organism=Homo sapiens, GI239788483, Length=188, Percent_Identity=28.1914893617021, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI239788462, Length=188, Percent_Identity=28.1914893617021, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI40807366, Length=181, Percent_Identity=30.3867403314917, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1789660, Length=231, Percent_Identity=30.7359307359307, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1788462, Length=257, Percent_Identity=29.5719844357977, Blast_Score=105, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17507177, Length=233, Percent_Identity=29.1845493562232, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17543114, Length=205, Percent_Identity=26.3414634146341, Blast_Score=78, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI25144369, Length=205, Percent_Identity=28.780487804878, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323560, Length=175, Percent_Identity=28.5714285714286, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24585320, Length=208, Percent_Identity=34.6153846153846, Blast_Score=105, Evalue=6e-23,
Organism=Drosophila melanogaster, GI19921524, Length=174, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24580595, Length=171, Percent_Identity=29.2397660818713, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19920448, Length=171, Percent_Identity=29.2397660818713, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35650; Mature: 35650

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDTTPSPVTTPRTPLPWPTGHTPLMLAPMQGLTNRALRALFINWVRPDVVFTEFMRVNP
CCCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
GGARQPIRAGDLREVAAVEGGVPLVVQLIGHDHKALVAAARTAQEAGAVHINLNLGCPYG
CCCCCCCCCCCHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEEEEECCCCH
RMTSGLTGGGMLHRPEALEELIPALRRAIAGTFSVKVRAGYDDSGQIMTLLPLFEEAGVD
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEHHHHHCCCC
FLVLHPRTVVQEYTGHADHAVTAEVVRQTRLPVIANGDVRSAADGLRVLRKTGATGLMLG
EEEECCHHHHHHHCCCCCHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCCCEEEEC
RGAIADPLLFQRLRGKAPAEPTGEERVAMLKRYLGELLEGYGEIFCGDTQILGKIKGVMA
CCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEECCHHHHHHHHHHHH
TMDDPFLAKGLKQLKKARTVAAFASTLRDIQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHDTTPSPVTTPRTPLPWPTGHTPLMLAPMQGLTNRALRALFINWVRPDVVFTEFMRVNP
CCCCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
GGARQPIRAGDLREVAAVEGGVPLVVQLIGHDHKALVAAARTAQEAGAVHINLNLGCPYG
CCCCCCCCCCCHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHCCEEEEEEEECCCCH
RMTSGLTGGGMLHRPEALEELIPALRRAIAGTFSVKVRAGYDDSGQIMTLLPLFEEAGVD
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEEHHHHHCCCC
FLVLHPRTVVQEYTGHADHAVTAEVVRQTRLPVIANGDVRSAADGLRVLRKTGATGLMLG
EEEECCHHHHHHHCCCCCHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHHHCCCCEEEEC
RGAIADPLLFQRLRGKAPAEPTGEERVAMLKRYLGELLEGYGEIFCGDTQILGKIKGVMA
CCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCEEECCHHHHHHHHHHHH
TMDDPFLAKGLKQLKKARTVAAFASTLRDIQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]