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The map label for this gene is hypF2 [H]

Identifier: 78221346

GI number: 78221346

Start: 144317

End: 146599

Strand: Reverse

Name: hypF2 [H]

Synonym: Gmet_0119

Alternate gene names: 78221346

Gene position: 146599-144317 (Counterclockwise)

Preceding gene: 78221347

Following gene: 78221345

Centisome position: 3.67

GC content: 68.24

Gene sequence:

>2283_bases
ATGACCGATGCCAGGCGCACCAGGATAACCATTGAGGGGATTGTCCAGGGGGTCGGCTTCCGCCCCTTCGTCTTCCGCCT
CGCGGAGCACCACGGCATTGCCGGATGGGTGCAGAACACCACGGCGGGAGTTGTCGTTGAGGCGGAAGGGGATGCCCGGC
GCCTCGCCTCCTTCGTGGCTGACCTCACGGGGAAAGCCCCCCCTCTGGCTGTCATCGTTTCGCTTGCAACCGAAGAGATC
CCGCCGCAAGGGGGGACCGCCTTCGCCATCCGTTACAGCGAGGGGAGCGCCGGGCCGGTGCGGGTGGCCCCGGACGGCGA
CGTCTGCCCTGACTGCCTCCATGAGCTCTTCGATCCGGCCGACCGCCGCCATCGCTATCCCTTCATCACCTGCACCAACT
GCGGCCCCCGCTACTCCCTCATCACCGCTGTCCCCTACGATCGCCCCAACACCACCATGGCCCCCTTCCCCCTCTGCGAT
GCCTGCCGGACCGAGTACGAGGACCCCCGGAACCGCCGTTTCCATGCCCAGCCGGTGGCCTGTCCGGAGTGCGGTCCCCG
GCTCAGGCTCGTGGATGCCGACGACCGGGACCTGCCAGGGGATCCCGTGGAGGAGGCAGCAGGGCTCCTCTCCCAGGGAA
AGATCGTTGCCGTGAAAGGGATCGGCGGCTATCACCTGGCCGTGGACGCCCTGAACGACGGGGCGGTGCGGGAGCTGCGC
CGCCGCAAGGCCCGGGACGAGAAGCCCTTTGCGCTCATGGCCCCTGACCTGGCGGCGGTGCGGACCTTTGCCCTTCCCGA
CGCCGTGGAGGAGCGGCTCCTGGCCGCCCCCGAGCACCCCATCGTGCTCCTGCGCAAACGCCCCGACAATCCCGTCTCCC
CCCTGGCGGCCCCGGCCAACGGCTATTTCGGCACCATGCTCCCCTACGCGCCGCTCCACCATCTCCTCCTGCGGGACCGG
TTTACGGCCCTGGTCATGACGAGCGGCAACCTCTCCGATGAGCCCATTGCCTACCGGGACGCCGAGGCCCGGGAGCGGCT
TGCGTACATCGCCGACGCGTTCCTTGTCCACGACCGGGAGATCCATACCCGAACCGACGACTCGGTGATCCGGGTGTTCC
GGGGTAATCCCCTCTTCCTGCGCCGCTCCCGGGGATATGTGCCGCGGGAGGTGCGGATGCCCGTGGAGATTCCGGCTGTC
CTTGCCGTGGGGGCCGAGCTCAAGGGGACCATCTGCCTCGCCCGGGGAGACCGGGCCGTCATGAGCCAGCACATCGGCGA
CCTGAAGAATGCCGCCACCCTCGATTCTCTGGCGGAGACCACCGCGCACCTCCAGGGGCTCCTGGGGCTAACCCCGGAGC
TTGTTGCCCACGACCTCCACCCCGACTACCTCTCCACGGCCTTTGCCGAGGGGATCCCGGTGCTTCCGCGGGTGGCGGTG
CAGCACCACCACGCCCACCTGGCATCCTGCATGGCCGAGAACGGCCTCGACGGGGAGGTGATCGGCGTCATCTTCGACGG
CACCGGCTACGGCCGGGACGGCACGGTCTGGGGAGGGGAGTTTCTCGTGGGGGGATACGGGGGCTTCCGGCGGGCCGGCC
ACCTCCGGCAGGTTCCCCTTCTCGGCGGCGACGCGGCGGTGCGGGAGCCGTTTCGCATGGCCCTGTCCTACCTTTACGAT
GCCTTCGGTGCCGACTTTTTCGGCCTTTCCCTGCCGTGGCTTCGGGAGATCGGCGAAGCCGAGCGGCCTCTTTTTCTCCA
GATGCTGGAGCGCCGGATCAACTCCCCCCCCACCTCCAGCTGCGGTCGGCTCTTCGATGCCGTGGCGGCCCTCACGGGGG
TGCGGGGAAGGGTCTCCTACGAGGGGCAGGCGGCCATGGAACTGGAGGCTCTGGCCGAAGGGAGCGCTACGGCATCCATT
TATCCCTATGCCATTGTTCGCAACGACGTCAGCGCCGAGGTCGATTTCCGGCTGATGGTCCGGGCGCTCGCGGAGGATGC
GGCCGCCGGCCGGCCGGGGGGGGAGATGGCCCGGGCTTTCCACAATACCCTTGCCGCGGCAGTGACGGACCTCTGTGCCC
AGATTCGCGCTGTGGACGGCCTGGACCGTGTCGTCCTCTCCGGGGGCGTTTTCCAGAACAAGCTCCTGACGGAAGGGGTT
TTCCAGTCCCTGGCCGATCAGGGATTCCATGTCTACACCCACCGGCTCGTTCCTCCCAACGACGGGGGGCTGGCCCTGGG
GCAGGCGATAATCGCAGGATACGGAGCACGAAAAGGAGAATAG

Upstream 100 bases:

>100_bases
TTCGAGATCTCCAGCCGCACCGGAGAGGGGATGGATGCCTGGTACGGCTGGCTTGCCGGCCGCATCGCCGCCGTGAAGGG
ATAACGCCGCGTTTCGTCCC

Downstream 100 bases:

>100_bases
GATCTATGTGTCTCGGAGTACCCATGCAGGTGGTCAGCGTCGGCGACGGCGACATCGTTGCCGAGATCGACGGCGTGAGG
AAGGAAGCGAGCCTCATGCT

Product: hydrogenase maturation protein HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF2; [NiFe]-hydrogenase maturation factor hypF2; Hydrogenase maturation protein hypF2 [H]

Number of amino acids: Translated: 760; Mature: 759

Protein sequence:

>760_residues
MTDARRTRITIEGIVQGVGFRPFVFRLAEHHGIAGWVQNTTAGVVVEAEGDARRLASFVADLTGKAPPLAVIVSLATEEI
PPQGGTAFAIRYSEGSAGPVRVAPDGDVCPDCLHELFDPADRRHRYPFITCTNCGPRYSLITAVPYDRPNTTMAPFPLCD
ACRTEYEDPRNRRFHAQPVACPECGPRLRLVDADDRDLPGDPVEEAAGLLSQGKIVAVKGIGGYHLAVDALNDGAVRELR
RRKARDEKPFALMAPDLAAVRTFALPDAVEERLLAAPEHPIVLLRKRPDNPVSPLAAPANGYFGTMLPYAPLHHLLLRDR
FTALVMTSGNLSDEPIAYRDAEARERLAYIADAFLVHDREIHTRTDDSVIRVFRGNPLFLRRSRGYVPREVRMPVEIPAV
LAVGAELKGTICLARGDRAVMSQHIGDLKNAATLDSLAETTAHLQGLLGLTPELVAHDLHPDYLSTAFAEGIPVLPRVAV
QHHHAHLASCMAENGLDGEVIGVIFDGTGYGRDGTVWGGEFLVGGYGGFRRAGHLRQVPLLGGDAAVREPFRMALSYLYD
AFGADFFGLSLPWLREIGEAERPLFLQMLERRINSPPTSSCGRLFDAVAALTGVRGRVSYEGQAAMELEALAEGSATASI
YPYAIVRNDVSAEVDFRLMVRALAEDAAAGRPGGEMARAFHNTLAAAVTDLCAQIRAVDGLDRVVLSGGVFQNKLLTEGV
FQSLADQGFHVYTHRLVPPNDGGLALGQAIIAGYGARKGE

Sequences:

>Translated_760_residues
MTDARRTRITIEGIVQGVGFRPFVFRLAEHHGIAGWVQNTTAGVVVEAEGDARRLASFVADLTGKAPPLAVIVSLATEEI
PPQGGTAFAIRYSEGSAGPVRVAPDGDVCPDCLHELFDPADRRHRYPFITCTNCGPRYSLITAVPYDRPNTTMAPFPLCD
ACRTEYEDPRNRRFHAQPVACPECGPRLRLVDADDRDLPGDPVEEAAGLLSQGKIVAVKGIGGYHLAVDALNDGAVRELR
RRKARDEKPFALMAPDLAAVRTFALPDAVEERLLAAPEHPIVLLRKRPDNPVSPLAAPANGYFGTMLPYAPLHHLLLRDR
FTALVMTSGNLSDEPIAYRDAEARERLAYIADAFLVHDREIHTRTDDSVIRVFRGNPLFLRRSRGYVPREVRMPVEIPAV
LAVGAELKGTICLARGDRAVMSQHIGDLKNAATLDSLAETTAHLQGLLGLTPELVAHDLHPDYLSTAFAEGIPVLPRVAV
QHHHAHLASCMAENGLDGEVIGVIFDGTGYGRDGTVWGGEFLVGGYGGFRRAGHLRQVPLLGGDAAVREPFRMALSYLYD
AFGADFFGLSLPWLREIGEAERPLFLQMLERRINSPPTSSCGRLFDAVAALTGVRGRVSYEGQAAMELEALAEGSATASI
YPYAIVRNDVSAEVDFRLMVRALAEDAAAGRPGGEMARAFHNTLAAAVTDLCAQIRAVDGLDRVVLSGGVFQNKLLTEGV
FQSLADQGFHVYTHRLVPPNDGGLALGQAIIAGYGARKGE
>Mature_759_residues
TDARRTRITIEGIVQGVGFRPFVFRLAEHHGIAGWVQNTTAGVVVEAEGDARRLASFVADLTGKAPPLAVIVSLATEEIP
PQGGTAFAIRYSEGSAGPVRVAPDGDVCPDCLHELFDPADRRHRYPFITCTNCGPRYSLITAVPYDRPNTTMAPFPLCDA
CRTEYEDPRNRRFHAQPVACPECGPRLRLVDADDRDLPGDPVEEAAGLLSQGKIVAVKGIGGYHLAVDALNDGAVRELRR
RKARDEKPFALMAPDLAAVRTFALPDAVEERLLAAPEHPIVLLRKRPDNPVSPLAAPANGYFGTMLPYAPLHHLLLRDRF
TALVMTSGNLSDEPIAYRDAEARERLAYIADAFLVHDREIHTRTDDSVIRVFRGNPLFLRRSRGYVPREVRMPVEIPAVL
AVGAELKGTICLARGDRAVMSQHIGDLKNAATLDSLAETTAHLQGLLGLTPELVAHDLHPDYLSTAFAEGIPVLPRVAVQ
HHHAHLASCMAENGLDGEVIGVIFDGTGYGRDGTVWGGEFLVGGYGGFRRAGHLRQVPLLGGDAAVREPFRMALSYLYDA
FGADFFGLSLPWLREIGEAERPLFLQMLERRINSPPTSSCGRLFDAVAALTGVRGRVSYEGQAAMELEALAEGSATASIY
PYAIVRNDVSAEVDFRLMVRALAEDAAAGRPGGEMARAFHNTLAAAVTDLCAQIRAVDGLDRVVLSGGVFQNKLLTEGVF
QSLADQGFHVYTHRLVPPNDGGLALGQAIIAGYGARKGE

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=762, Percent_Identity=42.9133858267717, Blast_Score=472, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 82231; Mature: 82099

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDARRTRITIEGIVQGVGFRPFVFRLAEHHGIAGWVQNTTAGVVVEAEGDARRLASFVA
CCCCCHHEEEHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCEEEEECCCCHHHHHHHHH
DLTGKAPPLAVIVSLATEEIPPQGGTAFAIRYSEGSAGPVRVAPDGDVCPDCLHELFDPA
HHCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEECCCCCCCHHHHHHHCCCH
DRRHRYPFITCTNCGPRYSLITAVPYDRPNTTMAPFPLCDACRTEYEDPRNRRFHAQPVA
HHCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCEECCCCC
CPECGPRLRLVDADDRDLPGDPVEEAAGLLSQGKIVAVKGIGGYHLAVDALNDGAVRELR
CCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCEEEEEECCCCHHHHHHH
RRKARDEKPFALMAPDLAAVRTFALPDAVEERLLAAPEHPIVLLRKRPDNPVSPLAAPAN
HHHCCCCCCEEEECCCHHHHHHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCC
GYFGTMLPYAPLHHLLLRDRFTALVMTSGNLSDEPIAYRDAEARERLAYIADAFLVHDRE
CCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCEECCHHHHHHHHHHHHHHHEECCH
IHTRTDDSVIRVFRGNPLFLRRSRGYVPREVRMPVEIPAVLAVGAELKGTICLARGDRAV
HCCCCCCCEEEEECCCEEEEECCCCCCCHHCCCCCCCCHHHHCCCCCCCEEEEECCCHHH
MSQHIGDLKNAATLDSLAETTAHLQGLLGLTPELVAHDLHPDYLSTAFAEGIPVLPRVAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHCCCCCCHHHHH
QHHHAHLASCMAENGLDGEVIGVIFDGTGYGRDGTVWGGEFLVGGYGGFRRAGHLRQVPL
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEECCCEEEECCCCHHHCCCCEECCC
LGGDAAVREPFRMALSYLYDAFGADFFGLSLPWLREIGEAERPLFLQMLERRINSPPTSS
CCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCHH
CGRLFDAVAALTGVRGRVSYEGQAAMELEALAEGSATASIYPYAIVRNDVSAEVDFRLMV
HHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCCCEECEEEEEECCCCCCHHHHHHH
RALAEDAAAGRPGGEMARAFHNTLAAAVTDLCAQIRAVDGLDRVVLSGGVFQNKLLTEGV
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHH
FQSLADQGFHVYTHRLVPPNDGGLALGQAIIAGYGARKGE
HHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TDARRTRITIEGIVQGVGFRPFVFRLAEHHGIAGWVQNTTAGVVVEAEGDARRLASFVA
CCCCHHEEEHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCEEEEECCCCHHHHHHHHH
DLTGKAPPLAVIVSLATEEIPPQGGTAFAIRYSEGSAGPVRVAPDGDVCPDCLHELFDPA
HHCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEECCCCCCCHHHHHHHCCCH
DRRHRYPFITCTNCGPRYSLITAVPYDRPNTTMAPFPLCDACRTEYEDPRNRRFHAQPVA
HHCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCEECCCCC
CPECGPRLRLVDADDRDLPGDPVEEAAGLLSQGKIVAVKGIGGYHLAVDALNDGAVRELR
CCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCEEEEEECCCCHHHHHHH
RRKARDEKPFALMAPDLAAVRTFALPDAVEERLLAAPEHPIVLLRKRPDNPVSPLAAPAN
HHHCCCCCCEEEECCCHHHHHHHCCCHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCC
GYFGTMLPYAPLHHLLLRDRFTALVMTSGNLSDEPIAYRDAEARERLAYIADAFLVHDRE
CCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCCCEECCHHHHHHHHHHHHHHHEECCH
IHTRTDDSVIRVFRGNPLFLRRSRGYVPREVRMPVEIPAVLAVGAELKGTICLARGDRAV
HCCCCCCCEEEEECCCEEEEECCCCCCCHHCCCCCCCCHHHHCCCCCCCEEEEECCCHHH
MSQHIGDLKNAATLDSLAETTAHLQGLLGLTPELVAHDLHPDYLSTAFAEGIPVLPRVAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHCCCCCCHHHHH
QHHHAHLASCMAENGLDGEVIGVIFDGTGYGRDGTVWGGEFLVGGYGGFRRAGHLRQVPL
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEECCCEEEECCCCHHHCCCCEECCC
LGGDAAVREPFRMALSYLYDAFGADFFGLSLPWLREIGEAERPLFLQMLERRINSPPTSS
CCCCHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCHH
CGRLFDAVAALTGVRGRVSYEGQAAMELEALAEGSATASIYPYAIVRNDVSAEVDFRLMV
HHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCCCEECEEEEEECCCCCCHHHHHHH
RALAEDAAAGRPGGEMARAFHNTLAAAVTDLCAQIRAVDGLDRVVLSGGVFQNKLLTEGV
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHH
FQSLADQGFHVYTHRLVPPNDGGLALGQAIIAGYGARKGE
HHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9799289; 12948488 [H]