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The map label for this gene is hypE [H]

Identifier: 78221343

GI number: 78221343

Start: 141947

End: 142996

Strand: Reverse

Name: hypE [H]

Synonym: Gmet_0116

Alternate gene names: 78221343

Gene position: 142996-141947 (Counterclockwise)

Preceding gene: 78221344

Following gene: 78221342

Centisome position: 3.58

GC content: 67.43

Gene sequence:

>1050_bases
GTGACCAGCAGCAAAGAATATGACCGCTCGGAGAATCCTGTGAACAAAGACCTCATCCTCCTGGGCCACGGCAGCGGCGG
GAAGCTTTCCCACCAGCTCCTGGACGACCTCATCATCCCGACCCTCTCGGGCATCCCCCTCGCCGGGCAGAACGACGCGG
CGGTGGTGGAGCATGGCGGCCAGAGGCTCGCCTTCACCACCGACTCCTACGTGGTGGACCCCATCTTCTTCCCCGGCGGC
AACATCGGAAGCCTGGCCGTGAACGGCACCGTGAACGACCTGGCCATGATGGGGGCGCGCCCCCTCTTCATCAGCGCCGG
CCTCATTGTCGAGGAAGGGTTCAGCCGGACCGAACTGGCCGGGATCCTCGCCTCCATGCGGGGTGCTGCCGACGCCGCCG
GGGTCAGGATCGTCACCGGCGATACCAAGGTGGTCCCCCGGGGGAAGGCGGACCGAATCTTCATCAACACCTCCGGCATC
GGGGCCGTGGAGCACGATTTCGCCATTAACGGCGCCAACGCCCGGGCCGGCGACGTGGTCATCATCAACGGCACCATCGG
CGACCATGGTATCGCGGTCATGGCCGAGCGGGAGGGGCTTGACCTCCGGACCGCCATCCGGAGCGACTGCGCGGCCCTGA
ACGACCTCGTGGCCGAGATCATCGCTGCAGGGGGCGACGCGGTCCACGTTCTGCGGGACCCGACCCGGGGGGGAGTCGCC
ACGACCCTCAAGGAAATCGCCCTTCAGTCCGATAAGACTATTACCCTCCGGGAGGAGACCCTCCCCCTCAACGGAGCGGT
GAAGGGGGTCTGCTCGATCCTCGGTCTCGATCCCCTCTACGTGGCCAACGAGGGGAAGCTTCTGGCCGTGGTGGCCCCCG
AGGCCGCGGAGAGGGTGCTGACCCGCATCAAGGGGCATCGCCTCGGGAGGGATGCCGCCATCATCGGCCATGTGGCTGAG
GGGGGAGGCCGGGTACAGATGGAGACCGCCGTGGGCGGCATGCGGGCCGTGGAGATGCTGGCCGGAGAGCAGCTCCCCCG
CATCTGCTAG

Upstream 100 bases:

>100_bases
ACTGTCCCCTCTTTGGCGGCGCCTGCACCCCCGAGTCCCCCGTGGGGGCCTGCATGGTCTCCTCCGAGGGGACCTGCGCG
GCGGCGTACAAGTATGGGCA

Downstream 100 bases:

>100_bases
CAGTCAGCATCCATCAGCAGGATTGTTTTGTGCTATCTTTACTTCAGTGTTGAAGTATCTCAGGGGGGAAATGTCATGCC
TAACCATCACACCATGTCCC

Product: hydrogenase expression/formation protein HypE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 348

Protein sequence:

>349_residues
MTSSKEYDRSENPVNKDLILLGHGSGGKLSHQLLDDLIIPTLSGIPLAGQNDAAVVEHGGQRLAFTTDSYVVDPIFFPGG
NIGSLAVNGTVNDLAMMGARPLFISAGLIVEEGFSRTELAGILASMRGAADAAGVRIVTGDTKVVPRGKADRIFINTSGI
GAVEHDFAINGANARAGDVVIINGTIGDHGIAVMAEREGLDLRTAIRSDCAALNDLVAEIIAAGGDAVHVLRDPTRGGVA
TTLKEIALQSDKTITLREETLPLNGAVKGVCSILGLDPLYVANEGKLLAVVAPEAAERVLTRIKGHRLGRDAAIIGHVAE
GGGRVQMETAVGGMRAVEMLAGEQLPRIC

Sequences:

>Translated_349_residues
MTSSKEYDRSENPVNKDLILLGHGSGGKLSHQLLDDLIIPTLSGIPLAGQNDAAVVEHGGQRLAFTTDSYVVDPIFFPGG
NIGSLAVNGTVNDLAMMGARPLFISAGLIVEEGFSRTELAGILASMRGAADAAGVRIVTGDTKVVPRGKADRIFINTSGI
GAVEHDFAINGANARAGDVVIINGTIGDHGIAVMAEREGLDLRTAIRSDCAALNDLVAEIIAAGGDAVHVLRDPTRGGVA
TTLKEIALQSDKTITLREETLPLNGAVKGVCSILGLDPLYVANEGKLLAVVAPEAAERVLTRIKGHRLGRDAAIIGHVAE
GGGRVQMETAVGGMRAVEMLAGEQLPRIC
>Mature_348_residues
TSSKEYDRSENPVNKDLILLGHGSGGKLSHQLLDDLIIPTLSGIPLAGQNDAAVVEHGGQRLAFTTDSYVVDPIFFPGGN
IGSLAVNGTVNDLAMMGARPLFISAGLIVEEGFSRTELAGILASMRGAADAAGVRIVTGDTKVVPRGKADRIFINTSGIG
AVEHDFAINGANARAGDVVIINGTIGDHGIAVMAEREGLDLRTAIRSDCAALNDLVAEIIAAGGDAVHVLRDPTRGGVAT
TLKEIALQSDKTITLREETLPLNGAVKGVCSILGLDPLYVANEGKLLAVVAPEAAERVLTRIKGHRLGRDAAIIGHVAEG
GGRVQMETAVGGMRAVEMLAGEQLPRIC

Specific function: Unknown

COG id: COG0309

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypE family [H]

Homologues:

Organism=Escherichia coli, GI226510967, Length=336, Percent_Identity=46.4285714285714, Blast_Score=275, Evalue=4e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR011854
- InterPro:   IPR016188 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: NA

Molecular weight: Translated: 36198; Mature: 36067

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSKEYDRSENPVNKDLILLGHGSGGKLSHQLLDDLIIPTLSGIPLAGQNDAAVVEHGG
CCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHEECCC
QRLAFTTDSYVVDPIFFPGGNIGSLAVNGTVNDLAMMGARPLFISAGLIVEEGFSRTELA
CEEEEEECCEEECEEEECCCCCCEEEECCCHHHHHHHCCCCEEEECCEEEECCCCHHHHH
GILASMRGAADAAGVRIVTGDTKVVPRGKADRIFINTSGIGAVEHDFAINGANARAGDVV
HHHHHHCCCCCCCCEEEEECCCEEECCCCCCEEEEECCCCCCEECCEEEECCCCCCCCEE
IINGTIGDHGIAVMAEREGLDLRTAIRSDCAALNDLVAEIIAAGGDAVHVLRDPTRGGVA
EEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHH
TTLKEIALQSDKTITLREETLPLNGAVKGVCSILGLDPLYVANEGKLLAVVAPEAAERVL
HHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCEEEEECCHHHHHHH
TRIKGHRLGRDAAIIGHVAEGGGRVQMETAVGGMRAVEMLAGEQLPRIC
HHHHCCCCCCCEEEEEEEECCCCEEEEEHHHCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TSSKEYDRSENPVNKDLILLGHGSGGKLSHQLLDDLIIPTLSGIPLAGQNDAAVVEHGG
CCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHEECCC
QRLAFTTDSYVVDPIFFPGGNIGSLAVNGTVNDLAMMGARPLFISAGLIVEEGFSRTELA
CEEEEEECCEEECEEEECCCCCCEEEECCCHHHHHHHCCCCEEEECCEEEECCCCHHHHH
GILASMRGAADAAGVRIVTGDTKVVPRGKADRIFINTSGIGAVEHDFAINGANARAGDVV
HHHHHHCCCCCCCCEEEEECCCEEECCCCCCEEEEECCCCCCEECCEEEECCCCCCCCEE
IINGTIGDHGIAVMAEREGLDLRTAIRSDCAALNDLVAEIIAAGGDAVHVLRDPTRGGVA
EEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHH
TTLKEIALQSDKTITLREETLPLNGAVKGVCSILGLDPLYVANEGKLLAVVAPEAAERVL
HHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHCCCCEEEECCCCEEEEECCHHHHHHH
TRIKGHRLGRDAAIIGHVAEGGGRVQMETAVGGMRAVEMLAGEQLPRIC
HHHHCCCCCCCEEEEEEEECCCCEEEEEHHHCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8510650; 7906310; 12597275 [H]