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The map label for this gene is crp [H]

Identifier: 78221319

GI number: 78221319

Start: 116108

End: 116800

Strand: Reverse

Name: crp [H]

Synonym: Gmet_0092

Alternate gene names: 78221319

Gene position: 116800-116108 (Counterclockwise)

Preceding gene: 78221320

Following gene: 78221316

Centisome position: 2.92

GC content: 64.65

Gene sequence:

>693_bases
GTGGAACTAACCGAGATCCTGAAAAAATCCCTCCTCTTTTCCGGCCTTGACGGCGAGAACCTGTCCGACGTGGCGGCCAT
CGCCACGCGGCGCCCCTTTGCCCGGGGAGAGTCCCTCTTCTCGGAAGGGGAGCCGGCCAGCGGCTTCTACCTTTTGGCCC
AGGGGAGCATGAAGCTCTGCAAGGTGTCCCCCGACGGGAAGGAAAAGGTCCTCCATTTCGTCCATCCCGGCGAGACCTTT
GCCGAGGCGGCTTTCTTCGGCGACGGGAAGTACCCGGCCGAGGCCCGGGCCACGGAAAAGGGGGAGGCGATCTTCTTTCC
GAGGGAGGGGTTCATGGGGATCCTGGAGCGGAACCCCCGCTTTTCCCTGAACCTCATCGTCTCCCTCTCCCTCATGCTCC
GCCGCTTCGCCCGCCAGATCGAGGAGCTCACCTTCGCCGAGGTGCCGGCCCGCCTCGCCGCCCACCTGGTGGAACTGGCC
GAACGCAAGTCCACCGCGTTCCAGGGAAAGACCTACCTGGATCTGGACATGAAGAAGGGAGAGCTGGCGTCGCGCCTCGG
CACCGTGAGCGAGACTCTGTCACGGGCCTTCCGGAAGCTGAAGGAGGAGGGGCTCATCGAGGTGGAAGGGAGCCGGGTGG
TGATTTTCGATATGGAGAAGCTGAAGGACGCCGCCGGGAGACGGCCGGCCTAG

Upstream 100 bases:

>100_bases
AACTGCGGTGATTCAGATGAGTGTCCACGATGTCATGGCACAAAAAGTTGTCCACGATGTCGTGGCACTCAACACCTAAT
CCATATTGAAAGGATATTCC

Downstream 100 bases:

>100_bases
CCTTGCTGCTCCCGGGTCACCCGCTTCCAGCGGTTGTAGACCCAGAGCCACTGGTCGGGGTGGCGGGCGATGTACTGCTC
GATGGCCCGGTTGAGAAGGC

Product: Crp/FNR family transcriptional regulator

Products: NA

Alternate protein names: cAMP receptor protein; cAMP regulatory protein [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MELTEILKKSLLFSGLDGENLSDVAAIATRRPFARGESLFSEGEPASGFYLLAQGSMKLCKVSPDGKEKVLHFVHPGETF
AEAAFFGDGKYPAEARATEKGEAIFFPREGFMGILERNPRFSLNLIVSLSLMLRRFARQIEELTFAEVPARLAAHLVELA
ERKSTAFQGKTYLDLDMKKGELASRLGTVSETLSRAFRKLKEEGLIEVEGSRVVIFDMEKLKDAAGRRPA

Sequences:

>Translated_230_residues
MELTEILKKSLLFSGLDGENLSDVAAIATRRPFARGESLFSEGEPASGFYLLAQGSMKLCKVSPDGKEKVLHFVHPGETF
AEAAFFGDGKYPAEARATEKGEAIFFPREGFMGILERNPRFSLNLIVSLSLMLRRFARQIEELTFAEVPARLAAHLVELA
ERKSTAFQGKTYLDLDMKKGELASRLGTVSETLSRAFRKLKEEGLIEVEGSRVVIFDMEKLKDAAGRRPA
>Mature_230_residues
MELTEILKKSLLFSGLDGENLSDVAAIATRRPFARGESLFSEGEPASGFYLLAQGSMKLCKVSPDGKEKVLHFVHPGETF
AEAAFFGDGKYPAEARATEKGEAIFFPREGFMGILERNPRFSLNLIVSLSLMLRRFARQIEELTFAEVPARLAAHLVELA
ERKSTAFQGKTYLDLDMKKGELASRLGTVSETLSRAFRKLKEEGLIEVEGSRVVIFDMEKLKDAAGRRPA

Specific function: This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=180, Percent_Identity=23.8888888888889, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI1787595, Length=201, Percent_Identity=23.8805970149254, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25494; Mature: 25494

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELTEILKKSLLFSGLDGENLSDVAAIATRRPFARGESLFSEGEPASGFYLLAQGSMKLC
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHCHHHHCCCCCCCCEEEEECCCEEEE
KVSPDGKEKVLHFVHPGETFAEAAFFGDGKYPAEARATEKGEAIFFPREGFMGILERNPR
EECCCCHHHEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCHHHHCCCCC
FSLNLIVSLSLMLRRFARQIEELTFAEVPARLAAHLVELAERKSTAFQGKTYLDLDMKKG
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCH
ELASRLGTVSETLSRAFRKLKEEGLIEVEGSRVVIFDMEKLKDAAGRRPA
HHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEEEHHHHHHHCCCCCC
>Mature Secondary Structure
MELTEILKKSLLFSGLDGENLSDVAAIATRRPFARGESLFSEGEPASGFYLLAQGSMKLC
CCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHCHHHHCCCCCCCCEEEEECCCEEEE
KVSPDGKEKVLHFVHPGETFAEAAFFGDGKYPAEARATEKGEAIFFPREGFMGILERNPR
EECCCCHHHEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCCHHHHCCCCC
FSLNLIVSLSLMLRRFARQIEELTFAEVPARLAAHLVELAERKSTAFQGKTYLDLDMKKG
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCH
ELASRLGTVSETLSRAFRKLKEEGLIEVEGSRVVIFDMEKLKDAAGRRPA
HHHHHHHHHHHHHHHHHHHHHHCCCEEECCCEEEEEEHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]