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The map label for this gene is pcrA [H]

Identifier: 78221316

GI number: 78221316

Start: 112218

End: 114446

Strand: Reverse

Name: pcrA [H]

Synonym: Gmet_0089

Alternate gene names: 78221316

Gene position: 114446-112218 (Counterclockwise)

Preceding gene: 78221319

Following gene: 78221314

Centisome position: 2.86

GC content: 65.46

Gene sequence:

>2229_bases
ATGGACCTTCTGAAAAATCTCAACCCGCCCCAGAAGGAGGCGGTTCTCCACGGCGAGGGGCCGCTCCTGGTTCTGGCCGG
AGCCGGATCCGGCAAGACCCGGGTCATCGTCCACCGTATCGCTCACCTGATCTGCAACCTCGGCGTCCCCCCGTGGCAGA
TTCTGGCGGTTACCTTCACCAACAAGGCCGCCGGCGAGATGCGGGAGCGGGTGGAGCGGCTCGTGGGAGCCGGCGAGGCT
CCCCTCATCGCCACCTTCCACTCCACCTGCGCCCGGTTCCTGCGCCGCGATATCCACCACCTGGGGTATGACTCCTCCTT
CGCTATCTACGACGACAAGGACGCGGAAAAGCTTCTGAAGGAGGTGATCGCCGGCCTCGGCCTGGACGAGAAACGCTTCC
CGGCCCGCTCCTTTGCCGCCGCCATCGACGACTGCAAGAACCGGGGCATCCCCCCCGGGGAGATCCCCACCGGCGACTAC
ACCGGCGAGATGCTGGCCCGGGTCTACGGCGCCTACCAGGAGCGGCTGAAGAAGTGCAACGCCCTGGACTTCGGCGATCT
CCTCATGCTGACGGTCCGTCTCTTAGAAGAGCACCCCGAGGTGCTGGCCCGCTACCGGGACAAATATCGCTGGATCCTTG
TGGACGAGTATCAGGATACGAACCCGATTCAGTACCGGCTCGTGCGGCTTCTGGCCGGGGAGCGGCGCAACCTCTGCGTG
GTGGGGGACGATGACCAGTCAATCTATCGGTGGCGGGGGGCCGACATCCGGAACATCCTCGACTTCGAGAAGGATTTTCC
CGGCGTGGTCACAGTTAAGCTGGAGCAGAACTACCGTTCCACCCAAAACATCCTGGCCGCGGCCGGGGCCGTGGTGGCCA
AAAACCGCGGCCGCAAGGCCAAGACCCTCTGGAGCGAGAACCCGGCCGGCGAGGCCATTGTCTACCGGCGCCTCTCCGAC
GAGCGGGAGGAGGCCCGCTTCGTCTGCCGGGAGGTGGAGAAGCATGTGCGGCGCGGCGGGGATCTGCGGGACGTGGCGGT
CTTCTACCGGACCAACGCCCAGTCCCGGGTCATCGAGGACGCCATGGTGGCCGACGGGATTCCCTACCACATGGTGGGGG
GGATGCGGTTCTACGAGCGGATGGAGGTAAAGGACATCCTCGCCTACCTGAAGGTCCTCGTGAACCCCGCCGACGAGGTG
TCCCTCAAGCGGATCATCAACGTCCCGGCCCGGGGTATCGGCCACGCCACCGTGGACAAGGTGGCGGAGCTGGCATTCCG
CAAGGGGATCATTCTCTACGACGCCTTCAGCGAGGCGTCCACCAGCGGACTCCTCTCCACCGGCCCCCGGGGGAAAATCG
CCGGCTTCATGGCACTCATGGAGCAGTTTGCCGGCCTCGCCGGCACGGTACCCCTGTCGGAGCTCGCCTCCCGCATCATC
GACGGGACCGGCTACGCCGCCAAACTGAAGGAAGAGCGGAGCGACGAGGCCCAGGACCGCCTCGACAACCTCCAGGAACT
CTGTTCCGCCATGGAGGAGTTCGAGCGAACGAGCGACGAGAAGACCCTGGCCGCCTTCCTGGAGCAGGTGGCCCTCGTGT
CGGATCTGGAGCGGGGCGAGGGGAAGCGGGAATCGGCCACCCTCATGACCCTCCACGCGGCCAAGGGGCTCGAATTCCCA
GTGGTATTCATGATCGGAATGGAGGAGAAGCTTTTCCCACACGTCCGCTCCCTGGACGACCCCGAGGCCATGGAGGAGGA
GCGGCGCCTCTGCTACGTGGGGATGACCCGGGCGAGGGAGCGGCTGATCCTCACCAACGCCCGCCGCCGGCGACTCTTCG
GCCAGGACCAGGTGAACATGTCGTCCCGCTTCATCGCCGACGTTCCCCGGGATCTCCTGGATCTGGAAGACGAGTACCAG
TCCTCCTTCGGCTTCGGCGCAGGACGGGGGCTGGAGCGGGAGACAACAGCCGCCGAACCGACGCGCCACAACCTGGCGAG
CATCTTCGAAACGGAGATCGAGCCCGATTTCGACGACATCGAGGTGATCCCCGACGAGCCCGAGGACGGCGGCATCTGGC
TCGGCATGAAGGTGCGCCACGGCAAGTTCGGCGTCGGCGTCATCCGCAAGATCGAGGGGAAGGGGGACGACCAGAAGGTG
ATCGTCTGGTTCAACTCCGTGGGGCCGAAGAAGCTGTTGGTGCGGTTTGCAGGGTTGGAGCGGGTTTGA

Upstream 100 bases:

>100_bases
TTAACCTGGCAACTATACCACAAACATTCTTGACTAAATTCCCCCGGGCCGTAAATATGGGCCGTTGATCCAAGTCTCCA
CTGTGTGAAAAGGTACTCCC

Downstream 100 bases:

>100_bases
TCGCGGCCGTATTCAGGCCATCTTTGGCCGGTTCTCAATCGCACAATCCTCGACGTAGCGCTGCTACGCCTGCGGTTGTA
CTCATTCGGCCCGACCAAAT

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 742; Mature: 742

Protein sequence:

>742_residues
MDLLKNLNPPQKEAVLHGEGPLLVLAGAGSGKTRVIVHRIAHLICNLGVPPWQILAVTFTNKAAGEMRERVERLVGAGEA
PLIATFHSTCARFLRRDIHHLGYDSSFAIYDDKDAEKLLKEVIAGLGLDEKRFPARSFAAAIDDCKNRGIPPGEIPTGDY
TGEMLARVYGAYQERLKKCNALDFGDLLMLTVRLLEEHPEVLARYRDKYRWILVDEYQDTNPIQYRLVRLLAGERRNLCV
VGDDDQSIYRWRGADIRNILDFEKDFPGVVTVKLEQNYRSTQNILAAAGAVVAKNRGRKAKTLWSENPAGEAIVYRRLSD
EREEARFVCREVEKHVRRGGDLRDVAVFYRTNAQSRVIEDAMVADGIPYHMVGGMRFYERMEVKDILAYLKVLVNPADEV
SLKRIINVPARGIGHATVDKVAELAFRKGIILYDAFSEASTSGLLSTGPRGKIAGFMALMEQFAGLAGTVPLSELASRII
DGTGYAAKLKEERSDEAQDRLDNLQELCSAMEEFERTSDEKTLAAFLEQVALVSDLERGEGKRESATLMTLHAAKGLEFP
VVFMIGMEEKLFPHVRSLDDPEAMEEERRLCYVGMTRARERLILTNARRRRLFGQDQVNMSSRFIADVPRDLLDLEDEYQ
SSFGFGAGRGLERETTAAEPTRHNLASIFETEIEPDFDDIEVIPDEPEDGGIWLGMKVRHGKFGVGVIRKIEGKGDDQKV
IVWFNSVGPKKLLVRFAGLERV

Sequences:

>Translated_742_residues
MDLLKNLNPPQKEAVLHGEGPLLVLAGAGSGKTRVIVHRIAHLICNLGVPPWQILAVTFTNKAAGEMRERVERLVGAGEA
PLIATFHSTCARFLRRDIHHLGYDSSFAIYDDKDAEKLLKEVIAGLGLDEKRFPARSFAAAIDDCKNRGIPPGEIPTGDY
TGEMLARVYGAYQERLKKCNALDFGDLLMLTVRLLEEHPEVLARYRDKYRWILVDEYQDTNPIQYRLVRLLAGERRNLCV
VGDDDQSIYRWRGADIRNILDFEKDFPGVVTVKLEQNYRSTQNILAAAGAVVAKNRGRKAKTLWSENPAGEAIVYRRLSD
EREEARFVCREVEKHVRRGGDLRDVAVFYRTNAQSRVIEDAMVADGIPYHMVGGMRFYERMEVKDILAYLKVLVNPADEV
SLKRIINVPARGIGHATVDKVAELAFRKGIILYDAFSEASTSGLLSTGPRGKIAGFMALMEQFAGLAGTVPLSELASRII
DGTGYAAKLKEERSDEAQDRLDNLQELCSAMEEFERTSDEKTLAAFLEQVALVSDLERGEGKRESATLMTLHAAKGLEFP
VVFMIGMEEKLFPHVRSLDDPEAMEEERRLCYVGMTRARERLILTNARRRRLFGQDQVNMSSRFIADVPRDLLDLEDEYQ
SSFGFGAGRGLERETTAAEPTRHNLASIFETEIEPDFDDIEVIPDEPEDGGIWLGMKVRHGKFGVGVIRKIEGKGDDQKV
IVWFNSVGPKKLLVRFAGLERV
>Mature_742_residues
MDLLKNLNPPQKEAVLHGEGPLLVLAGAGSGKTRVIVHRIAHLICNLGVPPWQILAVTFTNKAAGEMRERVERLVGAGEA
PLIATFHSTCARFLRRDIHHLGYDSSFAIYDDKDAEKLLKEVIAGLGLDEKRFPARSFAAAIDDCKNRGIPPGEIPTGDY
TGEMLARVYGAYQERLKKCNALDFGDLLMLTVRLLEEHPEVLARYRDKYRWILVDEYQDTNPIQYRLVRLLAGERRNLCV
VGDDDQSIYRWRGADIRNILDFEKDFPGVVTVKLEQNYRSTQNILAAAGAVVAKNRGRKAKTLWSENPAGEAIVYRRLSD
EREEARFVCREVEKHVRRGGDLRDVAVFYRTNAQSRVIEDAMVADGIPYHMVGGMRFYERMEVKDILAYLKVLVNPADEV
SLKRIINVPARGIGHATVDKVAELAFRKGIILYDAFSEASTSGLLSTGPRGKIAGFMALMEQFAGLAGTVPLSELASRII
DGTGYAAKLKEERSDEAQDRLDNLQELCSAMEEFERTSDEKTLAAFLEQVALVSDLERGEGKRESATLMTLHAAKGLEFP
VVFMIGMEEKLFPHVRSLDDPEAMEEERRLCYVGMTRARERLILTNARRRRLFGQDQVNMSSRFIADVPRDLLDLEDEYQ
SSFGFGAGRGLERETTAAEPTRHNLASIFETEIEPDFDDIEVIPDEPEDGGIWLGMKVRHGKFGVGVIRKIEGKGDDQKV
IVWFNSVGPKKLLVRFAGLERV

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=751, Percent_Identity=40.745672436751, Blast_Score=542, Evalue=1e-155,
Organism=Escherichia coli, GI48994965, Length=645, Percent_Identity=41.8604651162791, Blast_Score=434, Evalue=1e-122,
Organism=Escherichia coli, GI1787196, Length=340, Percent_Identity=29.1176470588235, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322369, Length=769, Percent_Identity=28.4785435630689, Blast_Score=233, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6324477, Length=687, Percent_Identity=21.9796215429403, Blast_Score=121, Evalue=5e-28,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 83303; Mature: 83303

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLLKNLNPPQKEAVLHGEGPLLVLAGAGSGKTRVIVHRIAHLICNLGVPPWQILAVTFT
CCCCCCCCCCHHHCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEC
NKAAGEMRERVERLVGAGEAPLIATFHSTCARFLRRDIHHLGYDSSFAIYDDKDAEKLLK
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHH
EVIAGLGLDEKRFPARSFAAAIDDCKNRGIPPGEIPTGDYTGEMLARVYGAYQERLKKCN
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
ALDFGDLLMLTVRLLEEHPEVLARYRDKYRWILVDEYQDTNPIQYRLVRLLAGERRNLCV
CCCHHHHHHHHHHHHHHCHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEE
VGDDDQSIYRWRGADIRNILDFEKDFPGVVTVKLEQNYRSTQNILAAAGAVVAKNRGRKA
ECCCCCHHHEECCCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCH
KTLWSENPAGEAIVYRRLSDEREEARFVCREVEKHVRRGGDLRDVAVFYRTNAQSRVIED
HHHCCCCCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCHHHHHHH
AMVADGIPYHMVGGMRFYERMEVKDILAYLKVLVNPADEVSLKRIINVPARGIGHATVDK
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHH
VAELAFRKGIILYDAFSEASTSGLLSTGPRGKIAGFMALMEQFAGLAGTVPLSELASRII
HHHHHHHCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
DGTGYAAKLKEERSDEAQDRLDNLQELCSAMEEFERTSDEKTLAAFLEQVALVSDLERGE
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
GKRESATLMTLHAAKGLEFPVVFMIGMEEKLFPHVRSLDDPEAMEEERRLCYVGMTRARE
CCCCCCEEEEEEHHCCCCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHCEEEEEHHHHHH
RLILTNARRRRLFGQDQVNMSSRFIADVPRDLLDLEDEYQSSFGFGAGRGLERETTAAEP
HHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCC
TRHNLASIFETEIEPDFDDIEVIPDEPEDGGIWLGMKVRHGKFGVGVIRKIEGKGDDQKV
HHHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEEEEEECCCCCCHHHEEECCCCCCCEE
IVWFNSVGPKKLLVRFAGLERV
EEEECCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MDLLKNLNPPQKEAVLHGEGPLLVLAGAGSGKTRVIVHRIAHLICNLGVPPWQILAVTFT
CCCCCCCCCCHHHCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEC
NKAAGEMRERVERLVGAGEAPLIATFHSTCARFLRRDIHHLGYDSSFAIYDDKDAEKLLK
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHH
EVIAGLGLDEKRFPARSFAAAIDDCKNRGIPPGEIPTGDYTGEMLARVYGAYQERLKKCN
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
ALDFGDLLMLTVRLLEEHPEVLARYRDKYRWILVDEYQDTNPIQYRLVRLLAGERRNLCV
CCCHHHHHHHHHHHHHHCHHHHHHHHHCEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEE
VGDDDQSIYRWRGADIRNILDFEKDFPGVVTVKLEQNYRSTQNILAAAGAVVAKNRGRKA
ECCCCCHHHEECCCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCH
KTLWSENPAGEAIVYRRLSDEREEARFVCREVEKHVRRGGDLRDVAVFYRTNAQSRVIED
HHHCCCCCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCHHHHHHH
AMVADGIPYHMVGGMRFYERMEVKDILAYLKVLVNPADEVSLKRIINVPARGIGHATVDK
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHH
VAELAFRKGIILYDAFSEASTSGLLSTGPRGKIAGFMALMEQFAGLAGTVPLSELASRII
HHHHHHHCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
DGTGYAAKLKEERSDEAQDRLDNLQELCSAMEEFERTSDEKTLAAFLEQVALVSDLERGE
CCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
GKRESATLMTLHAAKGLEFPVVFMIGMEEKLFPHVRSLDDPEAMEEERRLCYVGMTRARE
CCCCCCEEEEEEHHCCCCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHCEEEEEHHHHHH
RLILTNARRRRLFGQDQVNMSSRFIADVPRDLLDLEDEYQSSFGFGAGRGLERETTAAEP
HHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHCCHHHHHHHCCCCCCCCCCCCCCCCCC
TRHNLASIFETEIEPDFDDIEVIPDEPEDGGIWLGMKVRHGKFGVGVIRKIEGKGDDQKV
HHHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEEEEEECCCCCCHHHEEECCCCCCCEE
IVWFNSVGPKKLLVRFAGLERV
EEEECCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA