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The map label for this gene is gatA

Identifier: 78221302

GI number: 78221302

Start: 91513

End: 92970

Strand: Reverse

Name: gatA

Synonym: Gmet_0075

Alternate gene names: 78221302

Gene position: 92970-91513 (Counterclockwise)

Preceding gene: 78221303

Following gene: 78221301

Centisome position: 2.33

GC content: 67.42

Gene sequence:

>1458_bases
ATGGAACTCTTTGATCTGACCATACACGAACTGCACGACCGGCTGAAGCGGAAGGAGCTCTCCTCCGTGGAGGCGACGAG
AGCGATGCTCGCCCGCATCGAGGCGGTGGACCCGAAGGTGAACGCCTACATCACGGTCACCCCCGAGGAGGCCCTGGCCG
CGGCAGAAGCGGCTGACCGGCGCATTGCCGCCGGCGACATGGCCCCGCTGACCGGCATCCCCGTCGCCCTCAAAGACATC
TTCGTCACCAAGGGGATCCGCACCACCTGCGCCTCGAAGATCCTCGACAACTTTGTCCCCCCCTACGACGGGACAGCGGT
GGCGAAGCTGAAGGAGGCCGGCGCGGTCATCGTGGGGAAACTAAACCAGGACGAGTTCGCCATGGGCTCCTCCGGCGAGT
CGAGCGCCTTCGGCCCCACCAGAAACCCCTGGAACCTGGAGTGCATCCCCGGCGGCTCCTCCAGCGGATCGGCGGCGGCC
ATCGCAGCCCGCACCGCCACGGCGACGCTCGGCACCGACACCGGCGGCTCCATCCGCCAGCCCGCCTCCCACTGCGGCTG
CGTGGGGCTTCGCCCCACCTACGGCCGGGTCTCCCGCTACGGGGTCATCGCCTACGCCTCCTCCCTGGACCAGGTGGGGC
CCGTGACCCGGGACGTGACCGACTGCGCCCTCATGCTCCAGGCCGTGGCCGGCCATGACCCCATGGATTCCACCAGCGTC
GAGGTGCCGGTCCCCGACTACGCAAAGGGGCTCACCGGCGACGTGAAGGGGCTGAAGCTGGGGCTTCCCAAGGAGTACTA
CATCGAGGGGCTCGACCCGGACGTGAAGAAGGCCCTGGACGAGGCCATCGAGACCTACCGGGGCCTCGGCGCCGAGTTTG
TGGATATCTCCCTCCCCCACACCGACTACGCCGTGGCCACCTATTACCTGATCGCCACCGCCGAGGCCAGCTCCAACCTG
GCCCGCTACGAGGGGGTCCGCTTCGGTCACCGGACGGAAGGGGCCGCAAACCTTATCGACATGTTCCGCAAGACCCGCTC
CGAAGGGTTCGGAGACGAGGTGAAGCGGCGGATCATGATCGGCACCTATGCCCTCTCCTCCGGCTACTACGACGCCTACT
ACCTGAAGGCCCAGAAAGTCCGCACCCTCATCATGCAGGACTTCCTCAAGGCCTTCGAGGCCGTGGACGCCATCCTCACC
CCCGTGGCCCCGACCCCGGCCTTCAAAATCGGCGAGAAGACGAGCGATCCGCTCCGGATGTACCTGTCGGACATCTTCAC
CATCCCCGTGAACCTGGCCGGCACCTGCGCCGTCTCCGTCCCCGCCGGGATGAGCGGCGCCGGACTCCCCATCGGCCTCC
AGCTCATCGGCCGCCCCTTCGGGGAGGAAACCATACTGCGCGCCGCCCACGCCTTCGAGCAGGCCACGGCGTGGCATACG
CAAAAGGCCGGGATATAG

Upstream 100 bases:

>100_bases
ACGTCGAGCTTCCACGGGTCGGTGGAGACGAGCGCCACCATAACGAACAACTCCCTGGGGAGGGAGACGGAAAAGTAAAA
TCGCACGCATCGGAGACCCC

Downstream 100 bases:

>100_bases
GGGCGATCACAAAAGGCGGGAATGGTGAGGGCTCTGAAATGAGCAAACGATTATCGAAAGACATTGCCGGTAAACACGTC
TCTCCCTTCGAGCAGATCAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MELFDLTIHELHDRLKRKELSSVEATRAMLARIEAVDPKVNAYITVTPEEALAAAEAADRRIAAGDMAPLTGIPVALKDI
FVTKGIRTTCASKILDNFVPPYDGTAVAKLKEAGAVIVGKLNQDEFAMGSSGESSAFGPTRNPWNLECIPGGSSSGSAAA
IAARTATATLGTDTGGSIRQPASHCGCVGLRPTYGRVSRYGVIAYASSLDQVGPVTRDVTDCALMLQAVAGHDPMDSTSV
EVPVPDYAKGLTGDVKGLKLGLPKEYYIEGLDPDVKKALDEAIETYRGLGAEFVDISLPHTDYAVATYYLIATAEASSNL
ARYEGVRFGHRTEGAANLIDMFRKTRSEGFGDEVKRRIMIGTYALSSGYYDAYYLKAQKVRTLIMQDFLKAFEAVDAILT
PVAPTPAFKIGEKTSDPLRMYLSDIFTIPVNLAGTCAVSVPAGMSGAGLPIGLQLIGRPFGEETILRAAHAFEQATAWHT
QKAGI

Sequences:

>Translated_485_residues
MELFDLTIHELHDRLKRKELSSVEATRAMLARIEAVDPKVNAYITVTPEEALAAAEAADRRIAAGDMAPLTGIPVALKDI
FVTKGIRTTCASKILDNFVPPYDGTAVAKLKEAGAVIVGKLNQDEFAMGSSGESSAFGPTRNPWNLECIPGGSSSGSAAA
IAARTATATLGTDTGGSIRQPASHCGCVGLRPTYGRVSRYGVIAYASSLDQVGPVTRDVTDCALMLQAVAGHDPMDSTSV
EVPVPDYAKGLTGDVKGLKLGLPKEYYIEGLDPDVKKALDEAIETYRGLGAEFVDISLPHTDYAVATYYLIATAEASSNL
ARYEGVRFGHRTEGAANLIDMFRKTRSEGFGDEVKRRIMIGTYALSSGYYDAYYLKAQKVRTLIMQDFLKAFEAVDAILT
PVAPTPAFKIGEKTSDPLRMYLSDIFTIPVNLAGTCAVSVPAGMSGAGLPIGLQLIGRPFGEETILRAAHAFEQATAWHT
QKAGI
>Mature_485_residues
MELFDLTIHELHDRLKRKELSSVEATRAMLARIEAVDPKVNAYITVTPEEALAAAEAADRRIAAGDMAPLTGIPVALKDI
FVTKGIRTTCASKILDNFVPPYDGTAVAKLKEAGAVIVGKLNQDEFAMGSSGESSAFGPTRNPWNLECIPGGSSSGSAAA
IAARTATATLGTDTGGSIRQPASHCGCVGLRPTYGRVSRYGVIAYASSLDQVGPVTRDVTDCALMLQAVAGHDPMDSTSV
EVPVPDYAKGLTGDVKGLKLGLPKEYYIEGLDPDVKKALDEAIETYRGLGAEFVDISLPHTDYAVATYYLIATAEASSNL
ARYEGVRFGHRTEGAANLIDMFRKTRSEGFGDEVKRRIMIGTYALSSGYYDAYYLKAQKVRTLIMQDFLKAFEAVDAILT
PVAPTPAFKIGEKTSDPLRMYLSDIFTIPVNLAGTCAVSVPAGMSGAGLPIGLQLIGRPFGEETILRAAHAFEQATAWHT
QKAGI

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=497, Percent_Identity=40.4426559356137, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI195972892, Length=508, Percent_Identity=24.0157480314961, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI166795287, Length=245, Percent_Identity=31.0204081632653, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=417, Percent_Identity=38.8489208633094, Blast_Score=275, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI17537465, Length=493, Percent_Identity=24.5436105476673, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71990152, Length=322, Percent_Identity=28.5714285714286, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17556264, Length=304, Percent_Identity=26.6447368421053, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=523, Percent_Identity=23.7093690248566, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17538254, Length=513, Percent_Identity=22.4171539961014, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=314, Percent_Identity=27.7070063694268, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=314, Percent_Identity=27.7070063694268, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=474, Percent_Identity=35.0210970464135, Blast_Score=245, Evalue=9e-66,
Organism=Saccharomyces cerevisiae, GI6319685, Length=473, Percent_Identity=28.9640591966173, Blast_Score=129, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6320448, Length=230, Percent_Identity=34.3478260869565, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648113, Length=451, Percent_Identity=40.7982261640798, Blast_Score=326, Evalue=2e-89,
Organism=Drosophila melanogaster, GI24644968, Length=511, Percent_Identity=25.6360078277886, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI161078093, Length=506, Percent_Identity=28.6561264822134, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24652985, Length=498, Percent_Identity=26.3052208835341, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19922090, Length=498, Percent_Identity=26.3052208835341, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24652981, Length=498, Percent_Identity=26.3052208835341, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24652983, Length=498, Percent_Identity=26.3052208835341, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI45550774, Length=493, Percent_Identity=22.7180527383367, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24648435, Length=493, Percent_Identity=22.7180527383367, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24648437, Length=493, Percent_Identity=22.7180527383367, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI21356731, Length=231, Percent_Identity=31.1688311688312, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24648441, Length=421, Percent_Identity=22.0902612826603, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24648439, Length=421, Percent_Identity=22.0902612826603, Blast_Score=82, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_GEOMG (Q39ZK0)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383049.1
- ProteinModelPortal:   Q39ZK0
- SMR:   Q39ZK0
- STRING:   Q39ZK0
- GeneID:   3739691
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0075
- NMPDR:   fig|269799.3.peg.138
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- PhylomeDB:   Q39ZK0
- ProtClustDB:   PRK00012
- BioCyc:   GMET269799:GMET_0075-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 51712; Mature: 51712

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 78-78 ACT_SITE 153-153 ACT_SITE 177-177

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELFDLTIHELHDRLKRKELSSVEATRAMLARIEAVDPKVNAYITVTPEEALAAAEAADR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCC
RIAAGDMAPLTGIPVALKDIFVTKGIRTTCASKILDNFVPPYDGTAVAKLKEAGAVIVGK
EECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCEEEEEE
LNQDEFAMGSSGESSAFGPTRNPWNLECIPGGSSSGSAAAIAARTATATLGTDTGGSIRQ
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEHHHEEEECCCCCCCCCC
PASHCGCVGLRPTYGRVSRYGVIAYASSLDQVGPVTRDVTDCALMLQAVAGHDPMDSTSV
CHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEE
EVPVPDYAKGLTGDVKGLKLGLPKEYYIEGLDPDVKKALDEAIETYRGLGAEFVDISLPH
EECCCHHHCCCCCCCCCEEECCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCC
TDYAVATYYLIATAEASSNLARYEGVRFGHRTEGAANLIDMFRKTRSEGFGDEVKRRIMI
CCHHHEEEEEEEECCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHEEE
GTYALSSGYYDAYYLKAQKVRTLIMQDFLKAFEAVDAILTPVAPTPAFKIGEKTSDPLRM
EEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
YLSDIFTIPVNLAGTCAVSVPAGMSGAGLPIGLQLIGRPFGEETILRAAHAFEQATAWHT
HHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEHHHHCCCCCHHHHHHHHHHHHHHHHHHH
QKAGI
HCCCC
>Mature Secondary Structure
MELFDLTIHELHDRLKRKELSSVEATRAMLARIEAVDPKVNAYITVTPEEALAAAEAADR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCC
RIAAGDMAPLTGIPVALKDIFVTKGIRTTCASKILDNFVPPYDGTAVAKLKEAGAVIVGK
EECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHCCEEEEEE
LNQDEFAMGSSGESSAFGPTRNPWNLECIPGGSSSGSAAAIAARTATATLGTDTGGSIRQ
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEEEHHHEEEECCCCCCCCCC
PASHCGCVGLRPTYGRVSRYGVIAYASSLDQVGPVTRDVTDCALMLQAVAGHDPMDSTSV
CHHHCCCCCCCCCCCHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEE
EVPVPDYAKGLTGDVKGLKLGLPKEYYIEGLDPDVKKALDEAIETYRGLGAEFVDISLPH
EECCCHHHCCCCCCCCCEEECCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCC
TDYAVATYYLIATAEASSNLARYEGVRFGHRTEGAANLIDMFRKTRSEGFGDEVKRRIMI
CCHHHEEEEEEEECCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHEEE
GTYALSSGYYDAYYLKAQKVRTLIMQDFLKAFEAVDAILTPVAPTPAFKIGEKTSDPLRM
EEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHH
YLSDIFTIPVNLAGTCAVSVPAGMSGAGLPIGLQLIGRPFGEETILRAAHAFEQATAWHT
HHHHHHHCCCCCCCEEEEECCCCCCCCCCCEEHHHHCCCCCHHHHHHHHHHHHHHHHHHH
QKAGI
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA