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The map label for this gene is leuD1 [H]

Identifier: 78189854

GI number: 78189854

Start: 2418042

End: 2418608

Strand: Reverse

Name: leuD1 [H]

Synonym: Cag_1899

Alternate gene names: 78189854

Gene position: 2418608-2418042 (Counterclockwise)

Preceding gene: 78189855

Following gene: 78189853

Centisome position: 94.03

GC content: 46.38

Gene sequence:

>567_bases
ATGGAAACAATCATTCAGGGAAAAGCCTACGTGTTAGGCAAGAATATTGATACCGATCAAATTATTCCCGCTGAACATTT
GGTTTATAGCCTTTCCGATCCAAAAGAGGTGGTGCTGTATGGTAAATACGCCCTTTCAGGCGTTCCACCCGAGCAAGGTG
GCTTACCACAAGGCAACATTCCTTTTGTGCGCGAAGGCGAAGTAACATCAGACTACACGATTATTGTTGCGGGAGCTAAC
TTTGGTTGCGGCTCCTCGCGTGAGCACGCCCCATTTGCCTTGCAAGTTGCAGGCGCAAAAGCCATTGTGGCTGAGTCATA
CGCTCGCATTTTTTACCGCAACTGCGTTGATGGTGGTTTTGTGATTCCATGCGAAAGCACAGAGTCGCTCGCACAAGCAG
TAAGCACAGGCGATGAGTTGAAAATTGACGTTATGGCAAACACACTCGAAAACCTTACAACCAAGAAAAGCTACAAGCTC
AATCCTCTTGGAAGCGTTTTTAGCATTGTGGAAGCTGGCGGCATTTTTGCTTACGCCAAAAAAGAAAATTTAATGGCGCA
AAGCTAA

Upstream 100 bases:

>100_bases
CTTGCTTCACCACTTACGGCAGCGGCATCAGCAATTACCGGCAAACTTACCGATCCGAGAGATTTTCTCTGATTGTAGGA
ACTAAGAGAATCACACTATT

Downstream 100 bases:

>100_bases
TGTTATGAGTTTATGCACAACCCCTATTGAGTTGTACGATACCACCCTGCGTGATGGCACGCAGGGTGAGCATATCAACC
TTTCTGTTCAAGATAAACTG

Product: 3-isopropylmalate dehydratase, small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase 1; IPMI 1; Isopropylmalate isomerase 1 [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
METIIQGKAYVLGKNIDTDQIIPAEHLVYSLSDPKEVVLYGKYALSGVPPEQGGLPQGNIPFVREGEVTSDYTIIVAGAN
FGCGSSREHAPFALQVAGAKAIVAESYARIFYRNCVDGGFVIPCESTESLAQAVSTGDELKIDVMANTLENLTTKKSYKL
NPLGSVFSIVEAGGIFAYAKKENLMAQS

Sequences:

>Translated_188_residues
METIIQGKAYVLGKNIDTDQIIPAEHLVYSLSDPKEVVLYGKYALSGVPPEQGGLPQGNIPFVREGEVTSDYTIIVAGAN
FGCGSSREHAPFALQVAGAKAIVAESYARIFYRNCVDGGFVIPCESTESLAQAVSTGDELKIDVMANTLENLTTKKSYKL
NPLGSVFSIVEAGGIFAYAKKENLMAQS
>Mature_188_residues
METIIQGKAYVLGKNIDTDQIIPAEHLVYSLSDPKEVVLYGKYALSGVPPEQGGLPQGNIPFVREGEVTSDYTIIVAGAN
FGCGSSREHAPFALQVAGAKAIVAESYARIFYRNCVDGGFVIPCESTESLAQAVSTGDELKIDVMANTLENLTTKKSYKL
NPLGSVFSIVEAGGIFAYAKKENLMAQS

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=150, Percent_Identity=32, Blast_Score=65, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321429, Length=109, Percent_Identity=38.5321100917431, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011824
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 20087; Mature: 20087

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METIIQGKAYVLGKNIDTDQIIPAEHLVYSLSDPKEVVLYGKYALSGVPPEQGGLPQGNI
CCCEECCCEEEEECCCCCCCCCCHHHEEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCC
PFVREGEVTSDYTIIVAGANFGCGSSREHAPFALQVAGAKAIVAESYARIFYRNCVDGGF
CEEECCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCE
VIPCESTESLAQAVSTGDELKIDVMANTLENLTTKKSYKLNPLGSVFSIVEAGGIFAYAK
EEECCCHHHHHHHHCCCCCEEEEEEHHHHHHHHCCCCEECCCHHHHHHHHHCCCEEEEEE
KENLMAQS
CCCCCCCC
>Mature Secondary Structure
METIIQGKAYVLGKNIDTDQIIPAEHLVYSLSDPKEVVLYGKYALSGVPPEQGGLPQGNI
CCCEECCCEEEEECCCCCCCCCCHHHEEEECCCCCEEEEEEEEEECCCCCCCCCCCCCCC
PFVREGEVTSDYTIIVAGANFGCGSSREHAPFALQVAGAKAIVAESYARIFYRNCVDGGF
CEEECCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCE
VIPCESTESLAQAVSTGDELKIDVMANTLENLTTKKSYKLNPLGSVFSIVEAGGIFAYAK
EEECCCHHHHHHHHCCCCCEEEEEEHHHHHHHHCCCCEECCCHHHHHHHHHCCCEEEEEE
KENLMAQS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]