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The map label for this gene is ptsI [H]

Identifier: 78189567

GI number: 78189567

Start: 2098294

End: 2100057

Strand: Reverse

Name: ptsI [H]

Synonym: Cag_1607

Alternate gene names: 78189567

Gene position: 2100057-2098294 (Counterclockwise)

Preceding gene: 78189568

Following gene: 78189565

Centisome position: 81.65

GC content: 45.63

Gene sequence:

>1764_bases
ATGAGTTCCCGCAGCCGAAGAGAAGAGCGAACCCCAAACGCATGTAACCAATCTGAAACCCTATACAATGGCATAGGTGC
CTCAAAAGGCATTGCCATTGGCGAGTGTTACGCCTTTATTAAAGAGGAGAACACGCATGAGCCAAGGGAACTCAATGAGA
AAAATTGCAAGGAGGAGGTTGAACGCTTTTTAACCGCCTTAAGCCGCTCTGAGCATGAGCTTAAAAAAATTGAGCAAGTA
ACCATCCGCAAGCTTGGCAAAAGCTATTCCAATCTTTTTCAAGCACAAATTATGATGCTGCACGATCCTGTGCTTGTGGA
GACTATTTCAAAACGCATTGTAAGTGAACGCAAAGGCGCTCATTTAGTGATTGATGATGAATTCAATAAATATCTTCAGC
ACTTTAAGAACTCCGACCATACGTTGTTTCAAGAGCGTGCCGATGATTTGCTTGATATTAAAAACCGCATTATTCGCAAT
CTCGATATTCGCAAACTGCACTCATGGATTCCCGAAGGGGTAATTGTTTGCTGCGATACCCTTTCACCTGCCGATATTAT
TCTTCTTAGCCGTAGCAACATCAAGGGTTTTGTAACCGCAACGGGTGGAAAAACTTCCCATATTTCGCTGATTTGCAAAT
CGCTCAAAATTCCGATGGTGGTGGGGCTGGGGCAAATTGCCGATAAAGTAGCAACGGGTATGGCGGTAATTATTGATGGT
GCCAATGGCACCGTTATTACCAATCCCTCGGCTGCAACGTTGGAGGAATATTTCCTTAAACAAAAAAATGAGCAACAATC
AAGCAGTAACCTTCAAGCCGCCATGCTGCCCGCAACCACACAGTGTGGGGTTCGGGTAAGCTTTTATGCTAACATTGATT
TTCGCGAAGAAGCCTTCTCTCTTGCTGCTGCTGGTGCCGAAGGGGTTGGCTTGTTCCGTAGCGAAAATCTTTTTACCGAA
GGCACAAAAACTCCAAAAGAAGAGGAGCAATTTACTTGTTACCGTGCTATTGCCGATGCCATTGCTCCCATGAGGCTTGA
TGTTCGGCTGTTTGATATTGGCGGCGATAAATTGCTCTATTCTCCCGTTAAAGAGATCAATCCAAATCTTGGCTGGCGCG
GCATTCGTATTTTGCTTGATTTGCCTGAAATTCTCGACACCCAAATTCGTGCCATCTTACAGGCAAACACTCATGGCAAT
ATTGATATTCTTATTCCCATGGTGATGTCGCTGCATGAAATTCGCACGGTTCGAGAATCGGTAGAACGGCAATTTGCGGA
GTTGCAAGCTGAGCGTGACGGGCAGATTACTCAACCCGGAATTGGCGCTATGATTGAGCTACCCGCTGCGGTTGAATTAA
TTGAAGAAATTACCCAATGTGTTGATTTTATTAGCATTGGCACCAATGACCTTACGCAATACACGCTTGCCGTTGACCGC
AACAATGTGATTGTGCAAGATTTATTTGACCGTTTTCATCCTGCCGTTATGCGCCAAGTGCATCGCATTATTCAGGTAGC
GAACAAAAATCACTGCTGTGCTATGATGTGTGGCGATATGGCATCCGATTCCCTTGCGCTGCCCTTTTTGCTTGGATGCG
GTTTGCGCAACTTTAGCGTTGTGGTTTCTGAGATTGCTGAACTTAAAGCGCATGTTGCTCGCTATGCACTTGCCGAAACC
GAAGCGCTTGCGCAAGAGTGTCTTGCTCTCAATAACCCAGCAGCAATTAAAGCGCGGCTTGAAGCTTTCCAAGCCGCCCA
TTAA

Upstream 100 bases:

>100_bases
GCTTGTATTCTCTGATTGAAGGAGAGAGAGTTGGAGAGAGCCTTGCACAGCTTTCGTGATAAACAATAGTATTTGAAAAT
TTAGTACCGCATTACCCACC

Downstream 100 bases:

>100_bases
AACGCATGAAGATAAAGGTGGATTGTTCACCGTTATCCTTTTTCGTGGTGCATTATGTAGAGCGCTAAAGCCTCCATAAT
ATCACCAGCCCAAAATCCTT

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 587; Mature: 586

Protein sequence:

>587_residues
MSSRSRREERTPNACNQSETLYNGIGASKGIAIGECYAFIKEENTHEPRELNEKNCKEEVERFLTALSRSEHELKKIEQV
TIRKLGKSYSNLFQAQIMMLHDPVLVETISKRIVSERKGAHLVIDDEFNKYLQHFKNSDHTLFQERADDLLDIKNRIIRN
LDIRKLHSWIPEGVIVCCDTLSPADIILLSRSNIKGFVTATGGKTSHISLICKSLKIPMVVGLGQIADKVATGMAVIIDG
ANGTVITNPSAATLEEYFLKQKNEQQSSSNLQAAMLPATTQCGVRVSFYANIDFREEAFSLAAAGAEGVGLFRSENLFTE
GTKTPKEEEQFTCYRAIADAIAPMRLDVRLFDIGGDKLLYSPVKEINPNLGWRGIRILLDLPEILDTQIRAILQANTHGN
IDILIPMVMSLHEIRTVRESVERQFAELQAERDGQITQPGIGAMIELPAAVELIEEITQCVDFISIGTNDLTQYTLAVDR
NNVIVQDLFDRFHPAVMRQVHRIIQVANKNHCCAMMCGDMASDSLALPFLLGCGLRNFSVVVSEIAELKAHVARYALAET
EALAQECLALNNPAAIKARLEAFQAAH

Sequences:

>Translated_587_residues
MSSRSRREERTPNACNQSETLYNGIGASKGIAIGECYAFIKEENTHEPRELNEKNCKEEVERFLTALSRSEHELKKIEQV
TIRKLGKSYSNLFQAQIMMLHDPVLVETISKRIVSERKGAHLVIDDEFNKYLQHFKNSDHTLFQERADDLLDIKNRIIRN
LDIRKLHSWIPEGVIVCCDTLSPADIILLSRSNIKGFVTATGGKTSHISLICKSLKIPMVVGLGQIADKVATGMAVIIDG
ANGTVITNPSAATLEEYFLKQKNEQQSSSNLQAAMLPATTQCGVRVSFYANIDFREEAFSLAAAGAEGVGLFRSENLFTE
GTKTPKEEEQFTCYRAIADAIAPMRLDVRLFDIGGDKLLYSPVKEINPNLGWRGIRILLDLPEILDTQIRAILQANTHGN
IDILIPMVMSLHEIRTVRESVERQFAELQAERDGQITQPGIGAMIELPAAVELIEEITQCVDFISIGTNDLTQYTLAVDR
NNVIVQDLFDRFHPAVMRQVHRIIQVANKNHCCAMMCGDMASDSLALPFLLGCGLRNFSVVVSEIAELKAHVARYALAET
EALAQECLALNNPAAIKARLEAFQAAH
>Mature_586_residues
SSRSRREERTPNACNQSETLYNGIGASKGIAIGECYAFIKEENTHEPRELNEKNCKEEVERFLTALSRSEHELKKIEQVT
IRKLGKSYSNLFQAQIMMLHDPVLVETISKRIVSERKGAHLVIDDEFNKYLQHFKNSDHTLFQERADDLLDIKNRIIRNL
DIRKLHSWIPEGVIVCCDTLSPADIILLSRSNIKGFVTATGGKTSHISLICKSLKIPMVVGLGQIADKVATGMAVIIDGA
NGTVITNPSAATLEEYFLKQKNEQQSSSNLQAAMLPATTQCGVRVSFYANIDFREEAFSLAAAGAEGVGLFRSENLFTEG
TKTPKEEEQFTCYRAIADAIAPMRLDVRLFDIGGDKLLYSPVKEINPNLGWRGIRILLDLPEILDTQIRAILQANTHGNI
DILIPMVMSLHEIRTVRESVERQFAELQAERDGQITQPGIGAMIELPAAVELIEEITQCVDFISIGTNDLTQYTLAVDRN
NVIVQDLFDRFHPAVMRQVHRIIQVANKNHCCAMMCGDMASDSLALPFLLGCGLRNFSVVVSEIAELKAHVARYALAETE
ALAQECLALNNPAAIKARLEAFQAAH

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=561, Percent_Identity=34.7593582887701, Blast_Score=311, Evalue=6e-86,
Organism=Escherichia coli, GI1789193, Length=571, Percent_Identity=31.6987740805604, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI48994992, Length=464, Percent_Identity=30.8189655172414, Blast_Score=214, Evalue=9e-57,
Organism=Escherichia coli, GI1788726, Length=531, Percent_Identity=30.3201506591337, Blast_Score=206, Evalue=4e-54,
Organism=Escherichia coli, GI1787994, Length=407, Percent_Identity=28.7469287469287, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 65292; Mature: 65161

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRSRREERTPNACNQSETLYNGIGASKGIAIGECYAFIKEENTHEPRELNEKNCKEEV
CCCCCCHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCHHCCHHHHHHHH
ERFLTALSRSEHELKKIEQVTIRKLGKSYSNLFQAQIMMLHDPVLVETISKRIVSERKGA
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
HLVIDDEFNKYLQHFKNSDHTLFQERADDLLDIKNRIIRNLDIRKLHSWIPEGVIVCCDT
EEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEECC
LSPADIILLSRSNIKGFVTATGGKTSHISLICKSLKIPMVVGLGQIADKVATGMAVIIDG
CCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHCCCCHHCCHHHHHHHHHCCEEEEEEC
ANGTVITNPSAATLEEYFLKQKNEQQSSSNLQAAMLPATTQCGVRVSFYANIDFREEAFS
CCCCEEECCCHHHHHHHHHHHCCCHHHCCCCEEEECCCCCCCCEEEEEEECCCHHHHHHH
LAAAGAEGVGLFRSENLFTEGTKTPKEEEQFTCYRAIADAIAPMRLDVRLFDIGGDKLLY
HHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCEEE
SPVKEINPNLGWRGIRILLDLPEILDTQIRAILQANTHGNIDILIPMVMSLHEIRTVRES
CCHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHH
VERQFAELQAERDGQITQPGIGAMIELPAAVELIEEITQCVDFISIGTNDLTQYTLAVDR
HHHHHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECC
NNVIVQDLFDRFHPAVMRQVHRIIQVANKNHCCAMMCGDMASDSLALPFLLGCGLRNFSV
CCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCCHHHH
VVSEIAELKAHVARYALAETEALAQECLALNNPAAIKARLEAFQAAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSRSRREERTPNACNQSETLYNGIGASKGIAIGECYAFIKEENTHEPRELNEKNCKEEV
CCCCCHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCHHCCHHHHHHHH
ERFLTALSRSEHELKKIEQVTIRKLGKSYSNLFQAQIMMLHDPVLVETISKRIVSERKGA
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCC
HLVIDDEFNKYLQHFKNSDHTLFQERADDLLDIKNRIIRNLDIRKLHSWIPEGVIVCCDT
EEEEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEECC
LSPADIILLSRSNIKGFVTATGGKTSHISLICKSLKIPMVVGLGQIADKVATGMAVIIDG
CCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHCCCCHHCCHHHHHHHHHCCEEEEEEC
ANGTVITNPSAATLEEYFLKQKNEQQSSSNLQAAMLPATTQCGVRVSFYANIDFREEAFS
CCCCEEECCCHHHHHHHHHHHCCCHHHCCCCEEEECCCCCCCCEEEEEEECCCHHHHHHH
LAAAGAEGVGLFRSENLFTEGTKTPKEEEQFTCYRAIADAIAPMRLDVRLFDIGGDKLLY
HHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCEEE
SPVKEINPNLGWRGIRILLDLPEILDTQIRAILQANTHGNIDILIPMVMSLHEIRTVRES
CCHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHH
VERQFAELQAERDGQITQPGIGAMIELPAAVELIEEITQCVDFISIGTNDLTQYTLAVDR
HHHHHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECC
NNVIVQDLFDRFHPAVMRQVHRIIQVANKNHCCAMMCGDMASDSLALPFLLGCGLRNFSV
CCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCCHHHH
VVSEIAELKAHVARYALAETEALAQECLALNNPAAIKARLEAFQAAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7686067; 9384377; 10568751; 2497294; 8449881; 1495386 [H]