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The map label for this gene is hemC

Identifier: 78189475

GI number: 78189475

Start: 1988591

End: 1989532

Strand: Reverse

Name: hemC

Synonym: Cag_1515

Alternate gene names: 78189475

Gene position: 1989532-1988591 (Counterclockwise)

Preceding gene: 78189476

Following gene: 78189474

Centisome position: 77.35

GC content: 46.18

Gene sequence:

>942_bases
TTGAAAAAACAGCTTATTATCGGCACCCGCTCCAGCCCCCTTGCATTGTGGCAGGCAGAATTTACGAAAGCAGAGCTTTC
GAAGCATTATCCTGATATGGAAATCACCTTGAAGTTGGTGAAAACCACCGGTGATGTGCTGCTTGATTCGCCACTTTCTA
AAATTGGCGATATGGGGCTTTTTACGAAGGATATTGAAAAGCATTTGCTTGCTCGCGAAATTGACTTGGCGGTTCACAGT
CTTAAAGATGTGCCAACCAGCACGCCTGAGGGATTAATTATCACCTCGTTTACCGAGCGTGAGGATACCCGCGACGTTAT
TATTTCAAAAGGTGGCGTTAAGCTGAAAGATTTGCCTCCTAATGCTCGTATGGCAACCAGCTCGCTTCGTCGTATGTCGC
AGTTGCTCAGTGTTCGCCCTGACCTTGATATTCGCGACATTCGTGGCAACCTCAACACTCGTTTCCAAAAGTTTGATGAT
GGCGAATTTGATGCCATGATGCTTGCTTACGCAGGTGTTTATCGCTTGAACTTTAGCGATCGCATCTCTGAAATTTTACC
GCACGACATGATGTTGCCAGCGGTTGGTCAAGGTGCGCTTGGTATTGAAACTCGTGTTGATGATGAGCAAACTCGTGAGA
TTGTGCGCATTCTTAACCACTCTACCACTGAGTATTGCTGCCGTGCAGAACGTGCATTGTTGCGCCATTTGCAAGGCGGA
TGCCAAATTCCAATTGGTGCTTATGCAACCTTCAGCAACGGTACCTTAAAGTTGTTGGCGTTTGTTGGCTCTGTTGATGG
AAAAACTGCGTTGCGTAACGAAATCACTAAAACGGGCTTAACCTCGCCAGAACAAGCTGAAGAGGCTGGTATTGCTTTAG
CTGAAGAGCTTTTAAAGCAAGGTGCGGATGCTATTTTGTCGCAAATTCGTAAAACTTGCTAA

Upstream 100 bases:

>100_bases
ATTCGATTCCAAGCCGTGTGAATTTGGTGCGTAACGTTTTTGACCTTGAAGAACCAAATCAGTCACATTTTTAAATCACC
CAATAAACTCTGTAACAGCC

Downstream 100 bases:

>100_bases
TGAAAAGCGTTCTTGTTACTCGCCCAAAAGATCAAGCAGCTTCGTTTGTAGAACAACTTGCAGCATATGGTTTGCTCTCG
GTGGTGTTCCCCACCATCGA

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKKQLIIGTRSSPLALWQAEFTKAELSKHYPDMEITLKLVKTTGDVLLDSPLSKIGDMGLFTKDIEKHLLAREIDLAVHS
LKDVPTSTPEGLIITSFTEREDTRDVIISKGGVKLKDLPPNARMATSSLRRMSQLLSVRPDLDIRDIRGNLNTRFQKFDD
GEFDAMMLAYAGVYRLNFSDRISEILPHDMMLPAVGQGALGIETRVDDEQTREIVRILNHSTTEYCCRAERALLRHLQGG
CQIPIGAYATFSNGTLKLLAFVGSVDGKTALRNEITKTGLTSPEQAEEAGIALAEELLKQGADAILSQIRKTC

Sequences:

>Translated_313_residues
MKKQLIIGTRSSPLALWQAEFTKAELSKHYPDMEITLKLVKTTGDVLLDSPLSKIGDMGLFTKDIEKHLLAREIDLAVHS
LKDVPTSTPEGLIITSFTEREDTRDVIISKGGVKLKDLPPNARMATSSLRRMSQLLSVRPDLDIRDIRGNLNTRFQKFDD
GEFDAMMLAYAGVYRLNFSDRISEILPHDMMLPAVGQGALGIETRVDDEQTREIVRILNHSTTEYCCRAERALLRHLQGG
CQIPIGAYATFSNGTLKLLAFVGSVDGKTALRNEITKTGLTSPEQAEEAGIALAEELLKQGADAILSQIRKTC
>Mature_313_residues
MKKQLIIGTRSSPLALWQAEFTKAELSKHYPDMEITLKLVKTTGDVLLDSPLSKIGDMGLFTKDIEKHLLAREIDLAVHS
LKDVPTSTPEGLIITSFTEREDTRDVIISKGGVKLKDLPPNARMATSSLRRMSQLLSVRPDLDIRDIRGNLNTRFQKFDD
GEFDAMMLAYAGVYRLNFSDRISEILPHDMMLPAVGQGALGIETRVDDEQTREIVRILNHSTTEYCCRAERALLRHLQGG
CQIPIGAYATFSNGTLKLLAFVGSVDGKTALRNEITKTGLTSPEQAEEAGIALAEELLKQGADAILSQIRKTC

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=334, Percent_Identity=39.2215568862275, Blast_Score=221, Evalue=6e-58,
Organism=Homo sapiens, GI66933009, Length=334, Percent_Identity=39.2215568862275, Blast_Score=221, Evalue=6e-58,
Organism=Escherichia coli, GI48994974, Length=305, Percent_Identity=49.1803278688525, Blast_Score=290, Evalue=7e-80,
Organism=Saccharomyces cerevisiae, GI6319996, Length=316, Percent_Identity=37.6582278481013, Blast_Score=196, Evalue=4e-51,
Organism=Drosophila melanogaster, GI20130425, Length=290, Percent_Identity=38.9655172413793, Blast_Score=190, Evalue=8e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_CHLCH (Q3AQF5)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_379813.1
- HSSP:   P06983
- ProteinModelPortal:   Q3AQF5
- SMR:   Q3AQF5
- STRING:   Q3AQF5
- GeneID:   3747148
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_1515
- eggNOG:   COG0181
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- ProtClustDB:   PRK00072
- BioCyc:   CCHL340177:CAG_1515-MONOMER
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 34640; Mature: 34640

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQLIIGTRSSPLALWQAEFTKAELSKHYPDMEITLKLVKTTGDVLLDSPLSKIGDMGL
CCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCHHEEEEEEHHCCHHHHCCHHHHHHCCCH
FTKDIEKHLLAREIDLAVHSLKDVPTSTPEGLIITSFTEREDTRDVIISKGGVKLKDLPP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHEEEECCCEEEECCCC
NARMATSSLRRMSQLLSVRPDLDIRDIRGNLNTRFQKFDDGEFDAMMLAYAGVYRLNFSD
CHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHEECHHH
RISEILPHDMMLPAVGQGALGIETRVDDEQTREIVRILNHSTTEYCCRAERALLRHLQGG
HHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
CQIPIGAYATFSNGTLKLLAFVGSVDGKTALRNEITKTGLTSPEQAEEAGIALAEELLKQ
CCCCCCCEEEECCCCEEEHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
GADAILSQIRKTC
HHHHHHHHHHHCC
>Mature Secondary Structure
MKKQLIIGTRSSPLALWQAEFTKAELSKHYPDMEITLKLVKTTGDVLLDSPLSKIGDMGL
CCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCHHEEEEEEHHCCHHHHCCHHHHHHCCCH
FTKDIEKHLLAREIDLAVHSLKDVPTSTPEGLIITSFTEREDTRDVIISKGGVKLKDLPP
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHEEEECCCEEEECCCC
NARMATSSLRRMSQLLSVRPDLDIRDIRGNLNTRFQKFDDGEFDAMMLAYAGVYRLNFSD
CHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHEECHHH
RISEILPHDMMLPAVGQGALGIETRVDDEQTREIVRILNHSTTEYCCRAERALLRHLQGG
HHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
CQIPIGAYATFSNGTLKLLAFVGSVDGKTALRNEITKTGLTSPEQAEEAGIALAEELLKQ
CCCCCCCEEEECCCCEEEHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
GADAILSQIRKTC
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA