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The map label for this gene is hemK [H]

Identifier: 78189388

GI number: 78189388

Start: 1894509

End: 1895402

Strand: Reverse

Name: hemK [H]

Synonym: Cag_1425

Alternate gene names: 78189388

Gene position: 1895402-1894509 (Counterclockwise)

Preceding gene: 78189393

Following gene: 78189387

Centisome position: 73.69

GC content: 51.9

Gene sequence:

>894_bases
ATGGAGAATCAAAAAGAGTGGCAGGTTATTGAGCTGCTTAAAACTACTACCGATTTTTTTGCCGCCAAAGAGATGAGTGA
ACCTCGCATAAGTGCGGAGCGTTTGCTTGGGCATGTGCTGCAAAAAAGCCGCTTGGAACTCTACCTCCATCACGATGCTC
CAATTTCGCCAAGCGAGCTTGAGCAGTTTCGTAGCTTTTGCCGCCAGCGCTTGCAAGGGCGCCCTGTGCAGTACATTACG
GGGGAACAATATTTTTATGGCGCTCCTTTTTTTGTGGATGAACGGGTGCTGATTCCTCGTCCTGAAACGGAGTTGCTTGT
TGAGCGGGCGCTTGAGGTAAGTGGCGTAAGTGCGCTTGCGGGTGAAGTGGCTGTGCTTGATGTTGGAACAGGAAGTGGCT
GCATTGCTGTTACATTGGCTACGTTGGCGCCAAACCTCCGTATTGTTGCGGTGGATCTATCGCCCGCAGCTTTGGATGTG
GCTCGCTTAAACGCTGAGCGGCATGGCGTAACCAATCGAATGACGTTTGTGCAAGCTGATATGACGTCGCCCTATTTTGC
CCAGCAACTGCCCTTTGCAACCTATCAGCTTATTATTTCTAATCCCCCCTATATTCCCAAAGCGGAATGGGCAACCTTAG
AGCGCGAGGTGCGCGACTTTGAACCTGAGCTTGCGCTTACCACGCCAAGTGGCATGGAGTGCTATCAAGCGTTAATCAGC
GCTGCGCCTACGTTACTTGCTGATGGCGGCACGTTAGCCTTAGAGCTACACGCCGATGGCGCACGCGCCGTTGCCACCAT
GCTGGAAAGTGCTGGCTTGCAAGAGGTGGCGCTAATGAAGGATTATGGAGGTTTTGAACGCATTATTACGGCGCGCAAGA
GCGAAAAAAGCTAA

Upstream 100 bases:

>100_bases
CTTTTCAGTGTAAAGCAATAAACGTTAGCTCACCCCTTTTCTTTCTTATAAAAAAAGGAAAAACCGCAACAAGCGCACTT
TTAATTATTATAGTAAGCTT

Downstream 100 bases:

>100_bases
CTGCTGGAATACCATTGATTTTTTTAAATTCAGCGCTTATTGGTTTTTAACTTAATAAAAAGAGATGTAATGGATTATCG
TGAACTAACAGCAAGAGAAC

Product: modification methylase HemK

Products: NA

Alternate protein names: M.XfaHemK2P [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MENQKEWQVIELLKTTTDFFAAKEMSEPRISAERLLGHVLQKSRLELYLHHDAPISPSELEQFRSFCRQRLQGRPVQYIT
GEQYFYGAPFFVDERVLIPRPETELLVERALEVSGVSALAGEVAVLDVGTGSGCIAVTLATLAPNLRIVAVDLSPAALDV
ARLNAERHGVTNRMTFVQADMTSPYFAQQLPFATYQLIISNPPYIPKAEWATLEREVRDFEPELALTTPSGMECYQALIS
AAPTLLADGGTLALELHADGARAVATMLESAGLQEVALMKDYGGFERIITARKSEKS

Sequences:

>Translated_297_residues
MENQKEWQVIELLKTTTDFFAAKEMSEPRISAERLLGHVLQKSRLELYLHHDAPISPSELEQFRSFCRQRLQGRPVQYIT
GEQYFYGAPFFVDERVLIPRPETELLVERALEVSGVSALAGEVAVLDVGTGSGCIAVTLATLAPNLRIVAVDLSPAALDV
ARLNAERHGVTNRMTFVQADMTSPYFAQQLPFATYQLIISNPPYIPKAEWATLEREVRDFEPELALTTPSGMECYQALIS
AAPTLLADGGTLALELHADGARAVATMLESAGLQEVALMKDYGGFERIITARKSEKS
>Mature_297_residues
MENQKEWQVIELLKTTTDFFAAKEMSEPRISAERLLGHVLQKSRLELYLHHDAPISPSELEQFRSFCRQRLQGRPVQYIT
GEQYFYGAPFFVDERVLIPRPETELLVERALEVSGVSALAGEVAVLDVGTGSGCIAVTLATLAPNLRIVAVDLSPAALDV
ARLNAERHGVTNRMTFVQADMTSPYFAQQLPFATYQLIISNPPYIPKAEWATLEREVRDFEPELALTTPSGMECYQALIS
AAPTLLADGGTLALELHADGARAVATMLESAGLQEVALMKDYGGFERIITARKSEKS

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=298, Percent_Identity=34.8993288590604, Blast_Score=140, Evalue=1e-33,
Organism=Escherichia coli, GI1787463, Length=266, Percent_Identity=34.5864661654135, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI87082085, Length=269, Percent_Identity=33.457249070632, Blast_Score=116, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324266, Length=184, Percent_Identity=32.0652173913043, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24582226, Length=244, Percent_Identity=31.1475409836066, Blast_Score=93, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 32815; Mature: 32815

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENQKEWQVIELLKTTTDFFAAKEMSEPRISAERLLGHVLQKSRLELYLHHDAPISPSEL
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHH
EQFRSFCRQRLQGRPVQYITGEQYFYGAPFFVDERVLIPRPETELLVERALEVSGVSALA
HHHHHHHHHHHCCCCEEEEECCCEEECCCEEECCEEECCCCCHHHHHHHHHHHCCHHHHC
GEVAVLDVGTGSGCIAVTLATLAPNLRIVAVDLSPAALDVARLNAERHGVTNRMTFVQAD
CCEEEEEECCCCCHHEEEHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEEC
MTSPYFAQQLPFATYQLIISNPPYIPKAEWATLEREVRDFEPELALTTPSGMECYQALIS
CCCCHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHH
AAPTLLADGGTLALELHADGARAVATMLESAGLQEVALMKDYGGFERIITARKSEKS
CCCCEEECCCEEEEEEECCCHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHCCCCC
>Mature Secondary Structure
MENQKEWQVIELLKTTTDFFAAKEMSEPRISAERLLGHVLQKSRLELYLHHDAPISPSEL
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHH
EQFRSFCRQRLQGRPVQYITGEQYFYGAPFFVDERVLIPRPETELLVERALEVSGVSALA
HHHHHHHHHHHCCCCEEEEECCCEEECCCEEECCEEECCCCCHHHHHHHHHHHCCHHHHC
GEVAVLDVGTGSGCIAVTLATLAPNLRIVAVDLSPAALDVARLNAERHGVTNRMTFVQAD
CCEEEEEECCCCCHHEEEHHHHCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEEEC
MTSPYFAQQLPFATYQLIISNPPYIPKAEWATLEREVRDFEPELALTTPSGMECYQALIS
CCCCHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHH
AAPTLLADGGTLALELHADGARAVATMLESAGLQEVALMKDYGGFERIITARKSEKS
CCCCEEECCCEEEEEEECCCHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]