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The map label for this gene is truA

Identifier: 78189343

GI number: 78189343

Start: 1844279

End: 1845010

Strand: Reverse

Name: truA

Synonym: Cag_1380

Alternate gene names: 78189343

Gene position: 1845010-1844279 (Counterclockwise)

Preceding gene: 78189346

Following gene: 78189342

Centisome position: 71.73

GC content: 50.0

Gene sequence:

>732_bases
ATGAGAACGATTCGGCTTGATATTGAATACGATGGAACCGACTTTGCAGGTTGGCAACGGCAATCGGGTACCATTCCAAC
CGTACAAGGCACCATTGAGAAGCTGTTAAGCCAAATTACGCAAGAAGAGGTGCTCTTAAACGGCGCAGGGCGTACCGATA
AAGGTGTTCATGCGCGGCAGCAAGTGGCAAGTTGCGCCCTCCATTCATCTATGGAATTATCACGCTTGGCGCATTCGTTG
AACTGCTTATTGCCGCCAACTATTCGCATCTGTAACGCTGTTCATGTAACTGATGATTTTCATGCCCGCTTTAGTGCTAC
GGAGCGCACCTATCGCTACTTTGTAAGCGAAACGCCATCAGCACTATGCAATCGCTTTACGGGGTGTGCTAACGCATTGC
TTGACGTTGGCGTTATGCAGCAGATGGCTGCCATGGTGGTTGGCGAGCACGACTTTACCGCTTTTTCGCGCGAAGAGCGC
GATAGCCCTGCAAAGCGTTGCAAGGTAACAAGCTGCCGTTGGCACCGCCTGCATGGCATGGTGGTGTTGCAAATAAGTGC
GAATCGCTTTTTACGCAGCATGGTACGTTACTTAGTTCACGCTATGTTGCAAGGGGGAAAAGGGCGTTTAGCTCCCACTC
TTTTTCAAGATATGGTTGAAAGTGGCACCTCAAGCTATCGGATGGTAGCCGCGCCAGCATCGGGGTTATTTTTATGGAGA
ATTGGCTATTAA

Upstream 100 bases:

>100_bases
AATGGCATGAAGATAACGATTGGAGGCGTTACTAAAAATGGCTTAGAATGGGTATAAGAGTTTATTATTGTGGCGCAATA
AATCCTCAAAAAATAAATTT

Downstream 100 bases:

>100_bases
TGTAGGGTTGGGGCAGGGTTGTTCATTCAGTGGGATTAAAAATCCGCTTAAAAAATTTTTGTTATTACATTACCTCCAAT
TTTGTGGCTTTTATACTATT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MRTIRLDIEYDGTDFAGWQRQSGTIPTVQGTIEKLLSQITQEEVLLNGAGRTDKGVHARQQVASCALHSSMELSRLAHSL
NCLLPPTIRICNAVHVTDDFHARFSATERTYRYFVSETPSALCNRFTGCANALLDVGVMQQMAAMVVGEHDFTAFSREER
DSPAKRCKVTSCRWHRLHGMVVLQISANRFLRSMVRYLVHAMLQGGKGRLAPTLFQDMVESGTSSYRMVAAPASGLFLWR
IGY

Sequences:

>Translated_243_residues
MRTIRLDIEYDGTDFAGWQRQSGTIPTVQGTIEKLLSQITQEEVLLNGAGRTDKGVHARQQVASCALHSSMELSRLAHSL
NCLLPPTIRICNAVHVTDDFHARFSATERTYRYFVSETPSALCNRFTGCANALLDVGVMQQMAAMVVGEHDFTAFSREER
DSPAKRCKVTSCRWHRLHGMVVLQISANRFLRSMVRYLVHAMLQGGKGRLAPTLFQDMVESGTSSYRMVAAPASGLFLWR
IGY
>Mature_243_residues
MRTIRLDIEYDGTDFAGWQRQSGTIPTVQGTIEKLLSQITQEEVLLNGAGRTDKGVHARQQVASCALHSSMELSRLAHSL
NCLLPPTIRICNAVHVTDDFHARFSATERTYRYFVSETPSALCNRFTGCANALLDVGVMQQMAAMVVGEHDFTAFSREER
DSPAKRCKVTSCRWHRLHGMVVLQISANRFLRSMVRYLVHAMLQGGKGRLAPTLFQDMVESGTSSYRMVAAPASGLFLWR
IGY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=267, Percent_Identity=29.9625468164794, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI199559785, Length=258, Percent_Identity=26.7441860465116, Blast_Score=73, Evalue=2e-13,
Organism=Escherichia coli, GI1788657, Length=243, Percent_Identity=33.7448559670782, Blast_Score=120, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71985046, Length=252, Percent_Identity=25.7936507936508, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_CHLCH (Q3AQT7)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_379681.1
- ProteinModelPortal:   Q3AQT7
- SMR:   Q3AQT7
- STRING:   Q3AQT7
- GeneID:   3746564
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_1380
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   HSAIRRH
- ProtClustDB:   PRK00021
- BioCyc:   CCHL340177:CAG_1380-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 27159; Mature: 27159

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: ACT_SITE 53-53

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTIRLDIEYDGTDFAGWQRQSGTIPTVQGTIEKLLSQITQEEVLLNGAGRTDKGVHARQ
CCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
QVASCALHSSMELSRLAHSLNCLLPPTIRICNAVHVTDDFHARFSATERTYRYFVSETPS
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCEEEECCCHHHHHHHHHHHHHHHHHCCHH
ALCNRFTGCANALLDVGVMQQMAAMVVGEHDFTAFSREERDSPAKRCKVTSCRWHRLHGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHCCCHHHHHECCCHHHEEECCE
VVLQISANRFLRSMVRYLVHAMLQGGKGRLAPTLFQDMVESGTSSYRMVAAPASGLFLWR
EEEEECHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEE
IGY
ECC
>Mature Secondary Structure
MRTIRLDIEYDGTDFAGWQRQSGTIPTVQGTIEKLLSQITQEEVLLNGAGRTDKGVHARQ
CCEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
QVASCALHSSMELSRLAHSLNCLLPPTIRICNAVHVTDDFHARFSATERTYRYFVSETPS
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCEEEECCCHHHHHHHHHHHHHHHHHCCHH
ALCNRFTGCANALLDVGVMQQMAAMVVGEHDFTAFSREERDSPAKRCKVTSCRWHRLHGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHCCCHHHHHECCCHHHEEECCE
VVLQISANRFLRSMVRYLVHAMLQGGKGRLAPTLFQDMVESGTSSYRMVAAPASGLFLWR
EEEEECHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEE
IGY
ECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA