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The map label for this gene is mltD [H]

Identifier: 78189342

GI number: 78189342

Start: 1842366

End: 1844036

Strand: Reverse

Name: mltD [H]

Synonym: Cag_1379

Alternate gene names: 78189342

Gene position: 1844036-1842366 (Counterclockwise)

Preceding gene: 78189343

Following gene: 78189341

Centisome position: 71.69

GC content: 47.4

Gene sequence:

>1671_bases
ATGTTGTGGCTGCCCACAGCACTGCCGCTTGAGGCTGCTGAGCCTGCTCGCAATAACCCCAATGCGTTGCGGCGCTCCTC
TATTTCGGATGTTCTCGATAGCCTTGTAAACGCTACCTATTTTAAGGATGAGTACTTTACCGCACCGTCGCGTGAAGGTG
GCGTAAGTTTTCCATCAACCTTTGTGCCTCAATTTAGCGATTCCGTTTATAGCTCACGTATTGCGGCACTTCGGCGCAAA
ACACCCATGCCACTTGTTTATAACGCTCAAGTGAAAGGGTACATTCGCATGTATGCCGTTGAAAAGCGTAGCTATACCGC
TAAAATTTTAGGTTTAACAAAAATTTATTTTCCTCTCTTTGAAGAGAAGTTTGATACCTACAATGTGCCGCTTGAAATGA
AATACTTGGCGATTGTGGAATCGGCACTGAATCCCACAGCCGTCTCGCCAGCAGGTGCAAAAGGGTTGTGGCAGTTTATG
TATGGCACGGGTAAAATGTATGGCTTGGAGTCCTCCTCGTTTATTGAAGATCGTTACGATCCTTATAAATCAAGCGTTGC
AGCGGCTCGCCATTTGCGCGATTTATATCAAATTTATGGTGATTGGTTTTTAGCCCTTGCCGCTTACAATGCTGGTCCTG
GTAACGTTAATAAAGCAATTCGTCGCGCTGGTGGTGTAAAAGATTATTGGGCAATTTGGGACTATCTGCCAGCCGAAACA
CGAGGCTACGTGCCTGCCTTTATAGCAGTTCACTACATTATGAGCTACCATAATGAGCACAACATTCGTCCGCTTGAACC
AGCCTATCTCTATCGCGATATTGATACGTTGCGTACCTCACGCATGGTTACCTTTGAGCAAATCAGCGAAACGCTTGGTA
TTTCAGCCTCCGATTTAGAGTTCCTTAATCCGCAATACAAAATTGGAGTGATTCCTGCCTCTACGGGCAATGGTAACGTT
ATTCGGTTGCCTCGTCGGTATGTAGCCCAATTTCAACGCCGCGAACAAGAAATTTATGCTTATCGCTCCGCTCGCACTAT
GGAGCGTGAAGCGCTCTATGCTCGGCTTGAAAGTGTGCGAGCTGGTGCAGGTGAGCAAAGTAGTGAAAGCAGTAAAGGCA
TGGGGAACCAGAAAATTCACATTGTACAGCGAGGTGAAACGCTTGGCTCGGTTGCACGCTTGTATCGCACCTACATTAGC
CAGCTTATTGCATGGAATAATCTTGTAGATGCTGATATTATGGTTGGGCAACGCTTAGTAGTGTTTGGTGGCGAGGATAA
TAGTCCAGTTGCTGCACCTGAGCCACCAAAAAGCACTGTTCCACCGAAGGCTCCCCCTATAGAGCGTCAACCAACGGCAG
CACCAGAAGTGCGTGCAGCGGCTCCCCCCAAACGCATTGCTGTAACACGTTCAACCCAAACGGTTACTCGCGATGAGTTG
GTAGCACTTACTGAAACACCAACCGTGGCTACCGATAACACAAGCGCAAAGGCTGAGCCAATATTCCATGTAGTAGAGCC
AGGGCAAACGCTGTTTGCAATTGCTACACAACGCAAGGTAACGGTGAATCAGCTCATGCTTTGGAACAATCTTAAAAGTG
TTCAGATTAAAGCAGGGCAAAAGCTCATTGTTTCAAGCGATGGGCAAAGTGGGCGCGATAACAGTCAATAG

Upstream 100 bases:

>100_bases
GGTGTTTGCCACCCTTCAGTCCCTCATATTTAAGGAACGATCTCTTGTGAAGCATAGAAGTTTATCATCGTCATTATGCC
AAGTGGTAGCTTTCATTGGC

Downstream 100 bases:

>100_bases
GTTATCTCAAAAGTTGTAAAAAAATGGTAACTTTTTTTATTTCTTTACCCTCTATGGGTTACGAGATTTTGAGTAAGGGC
TTCTCTAATTAACCGATTAT

Product: peptidoglycan-binding LysM

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MLWLPTALPLEAAEPARNNPNALRRSSISDVLDSLVNATYFKDEYFTAPSREGGVSFPSTFVPQFSDSVYSSRIAALRRK
TPMPLVYNAQVKGYIRMYAVEKRSYTAKILGLTKIYFPLFEEKFDTYNVPLEMKYLAIVESALNPTAVSPAGAKGLWQFM
YGTGKMYGLESSSFIEDRYDPYKSSVAAARHLRDLYQIYGDWFLALAAYNAGPGNVNKAIRRAGGVKDYWAIWDYLPAET
RGYVPAFIAVHYIMSYHNEHNIRPLEPAYLYRDIDTLRTSRMVTFEQISETLGISASDLEFLNPQYKIGVIPASTGNGNV
IRLPRRYVAQFQRREQEIYAYRSARTMEREALYARLESVRAGAGEQSSESSKGMGNQKIHIVQRGETLGSVARLYRTYIS
QLIAWNNLVDADIMVGQRLVVFGGEDNSPVAAPEPPKSTVPPKAPPIERQPTAAPEVRAAAPPKRIAVTRSTQTVTRDEL
VALTETPTVATDNTSAKAEPIFHVVEPGQTLFAIATQRKVTVNQLMLWNNLKSVQIKAGQKLIVSSDGQSGRDNSQ

Sequences:

>Translated_556_residues
MLWLPTALPLEAAEPARNNPNALRRSSISDVLDSLVNATYFKDEYFTAPSREGGVSFPSTFVPQFSDSVYSSRIAALRRK
TPMPLVYNAQVKGYIRMYAVEKRSYTAKILGLTKIYFPLFEEKFDTYNVPLEMKYLAIVESALNPTAVSPAGAKGLWQFM
YGTGKMYGLESSSFIEDRYDPYKSSVAAARHLRDLYQIYGDWFLALAAYNAGPGNVNKAIRRAGGVKDYWAIWDYLPAET
RGYVPAFIAVHYIMSYHNEHNIRPLEPAYLYRDIDTLRTSRMVTFEQISETLGISASDLEFLNPQYKIGVIPASTGNGNV
IRLPRRYVAQFQRREQEIYAYRSARTMEREALYARLESVRAGAGEQSSESSKGMGNQKIHIVQRGETLGSVARLYRTYIS
QLIAWNNLVDADIMVGQRLVVFGGEDNSPVAAPEPPKSTVPPKAPPIERQPTAAPEVRAAAPPKRIAVTRSTQTVTRDEL
VALTETPTVATDNTSAKAEPIFHVVEPGQTLFAIATQRKVTVNQLMLWNNLKSVQIKAGQKLIVSSDGQSGRDNSQ
>Mature_556_residues
MLWLPTALPLEAAEPARNNPNALRRSSISDVLDSLVNATYFKDEYFTAPSREGGVSFPSTFVPQFSDSVYSSRIAALRRK
TPMPLVYNAQVKGYIRMYAVEKRSYTAKILGLTKIYFPLFEEKFDTYNVPLEMKYLAIVESALNPTAVSPAGAKGLWQFM
YGTGKMYGLESSSFIEDRYDPYKSSVAAARHLRDLYQIYGDWFLALAAYNAGPGNVNKAIRRAGGVKDYWAIWDYLPAET
RGYVPAFIAVHYIMSYHNEHNIRPLEPAYLYRDIDTLRTSRMVTFEQISETLGISASDLEFLNPQYKIGVIPASTGNGNV
IRLPRRYVAQFQRREQEIYAYRSARTMEREALYARLESVRAGAGEQSSESSKGMGNQKIHIVQRGETLGSVARLYRTYIS
QLIAWNNLVDADIMVGQRLVVFGGEDNSPVAAPEPPKSTVPPKAPPIERQPTAAPEVRAAAPPKRIAVTRSTQTVTRDEL
VALTETPTVATDNTSAKAEPIFHVVEPGQTLFAIATQRKVTVNQLMLWNNLKSVQIKAGQKLIVSSDGQSGRDNSQ

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=344, Percent_Identity=27.906976744186, Blast_Score=126, Evalue=4e-30,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 62059; Mature: 62059

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLWLPTALPLEAAEPARNNPNALRRSSISDVLDSLVNATYFKDEYFTAPSREGGVSFPST
CEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCHHCCCCCCCCCCCCCCH
FVPQFSDSVYSSRIAALRRKTPMPLVYNAQVKGYIRMYAVEKRSYTAKILGLTKIYFPLF
HCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEEEECCCCEEHHHHHHHHHHHHH
EEKFDTYNVPLEMKYLAIVESALNPTAVSPAGAKGLWQFMYGTGKMYGLESSSFIEDRYD
HHCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEECCCCCHHHHHHCC
PYKSSVAAARHLRDLYQIYGDWFLALAAYNAGPGNVNKAIRRAGGVKDYWAIWDYLPAET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCC
RGYVPAFIAVHYIMSYHNEHNIRPLEPAYLYRDIDTLRTSRMVTFEQISETLGISASDLE
CCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHH
FLNPQYKIGVIPASTGNGNVIRLPRRYVAQFQRREQEIYAYRSARTMEREALYARLESVR
HCCCCEEEEEEECCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGAGEQSSESSKGMGNQKIHIVQRGETLGSVARLYRTYISQLIAWNNLVDADIMVGQRLV
CCCCCCCCCHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHEECCEEE
VFGGEDNSPVAAPEPPKSTVPPKAPPIERQPTAAPEVRAAAPPKRIAVTRSTQTVTRDEL
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHE
VALTETPTVATDNTSAKAEPIFHVVEPGQTLFAIATQRKVTVNQLMLWNNLKSVQIKAGQ
EEEECCCEEECCCCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCC
KLIVSSDGQSGRDNSQ
EEEEECCCCCCCCCCC
>Mature Secondary Structure
MLWLPTALPLEAAEPARNNPNALRRSSISDVLDSLVNATYFKDEYFTAPSREGGVSFPST
CEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCHHCCCCCCCCCCCCCCH
FVPQFSDSVYSSRIAALRRKTPMPLVYNAQVKGYIRMYAVEKRSYTAKILGLTKIYFPLF
HCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEEEECCCCEEHHHHHHHHHHHHH
EEKFDTYNVPLEMKYLAIVESALNPTAVSPAGAKGLWQFMYGTGKMYGLESSSFIEDRYD
HHCCCCCCCCEEHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEECCCCCHHHHHHCC
PYKSSVAAARHLRDLYQIYGDWFLALAAYNAGPGNVNKAIRRAGGVKDYWAIWDYLPAET
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCC
RGYVPAFIAVHYIMSYHNEHNIRPLEPAYLYRDIDTLRTSRMVTFEQISETLGISASDLE
CCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCHH
FLNPQYKIGVIPASTGNGNVIRLPRRYVAQFQRREQEIYAYRSARTMEREALYARLESVR
HCCCCEEEEEEECCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGAGEQSSESSKGMGNQKIHIVQRGETLGSVARLYRTYISQLIAWNNLVDADIMVGQRLV
CCCCCCCCCHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCHHEECCEEE
VFGGEDNSPVAAPEPPKSTVPPKAPPIERQPTAAPEVRAAAPPKRIAVTRSTQTVTRDEL
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHE
VALTETPTVATDNTSAKAEPIFHVVEPGQTLFAIATQRKVTVNQLMLWNNLKSVQIKAGQ
EEEECCCEEECCCCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHCCCCEEEEECCC
KLIVSSDGQSGRDNSQ
EEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]