Click here to switch to the map view.

The map label for this gene is queA

Identifier: 78189254

GI number: 78189254

Start: 1752971

End: 1753996

Strand: Reverse

Name: queA

Synonym: Cag_1288

Alternate gene names: 78189254

Gene position: 1753996-1752971 (Counterclockwise)

Preceding gene: 78189255

Following gene: 78189252

Centisome position: 68.19

GC content: 48.83

Gene sequence:

>1026_bases
ATGCAGGTTGCTGATTTTGATTATCACCTTCCCGAAGAGCGGATTGCAAAATATCCGCCTTTGGAGCGAGGTTCTACTCG
TCTTTTAGTGCTTAACCGCCAAAGTGGGGCGTTAACGCACTCTCTTTACGCACATCTTGATACCTTTTTACAAGCGGGCG
ATTTACTGCTGTTGAACAATACGCGCGTGGTGCCTGCGCGTCTCTTTGCAACTCGAGCAACGGGAGCTACTATCGAATTA
ATGTTGCTTGAACGCCATCACCATCGTGAGCAATTAGTGCTCTATCGGGGCCAGCTCAAAGCGGGGGAGATGTTGCAGTC
GCATGGGCATACGTTGCTTGTGGAGGAAGTGCTGCCGCAAGGATTAGCTCGCCTTGCTTTAGCCGATGGTGGCGATTTGC
ACCAATTTTTTACCCAATTTGGGAGTGTACCCATTCCGCCATACCTCAAGCGCAATGCTGAAGCGGTTGATCGTGAGCGC
TATCAAACCGTTTTTGCAGCCCATTCTGGTTCAGTTGCAGCACCAACAGCATCACTCAATATGACGCCTGAGTTGCTTCA
GCGCCTTAAGAACAACGGTGTGGAAATTGCGCATATTACGCTCCACGTTGGGCTTGGTACCTTTTTGCCCATTCGTACCG
AGTCGTTGGAAGAGCATGTTATGCACCGTGAATCGTACTTGTTGCCTGCCGAAAGTGTTGCCGCCTTACAGCGCGTTAAA
GCAGATGGTGGACGTGTTGTTGCAGTTGGCACCACCGTTACGCGTGCCCTTGAACATTCGGCACCACGCATTTTACAATC
ATCGGCTCATGCAGAAATTGAAGGTGAAGCCGATATTTTTATTTATCCGGGCTATCGCTTTCAAATGGTAGATTTGCTGT
TAACTAATTTTCATGCACCACGCTCAACCGTGCTGATGCTTACGGCAGCTTGTGCAGGAACCGATAACCTGCGTGCAGCT
TATAGCGAAGCGGTATTGCAAGGCTATAATTTTTTAAGCTATGGCGATAGTATGCTTATTTGTTGA

Upstream 100 bases:

>100_bases
TAAAGGGGCACAAATTGCCGCGTTTGCTGATGCTGAAGGATTGCAAGCTCATGCTGAAGCGGTTCGTGTGCGCTTAAAAA
CCTTATAGTGGCGTTTTCTC

Downstream 100 bases:

>100_bases
CCCGCGTCAGCGCTCATTGCCAAGCGAATCTGAAAGAATTTCGGTAATCATTCAAATCTTCCTTAATTCCTAAGGATTTT
TGCGGAACTCCTCCTCGCCT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MQVADFDYHLPEERIAKYPPLERGSTRLLVLNRQSGALTHSLYAHLDTFLQAGDLLLLNNTRVVPARLFATRATGATIEL
MLLERHHHREQLVLYRGQLKAGEMLQSHGHTLLVEEVLPQGLARLALADGGDLHQFFTQFGSVPIPPYLKRNAEAVDRER
YQTVFAAHSGSVAAPTASLNMTPELLQRLKNNGVEIAHITLHVGLGTFLPIRTESLEEHVMHRESYLLPAESVAALQRVK
ADGGRVVAVGTTVTRALEHSAPRILQSSAHAEIEGEADIFIYPGYRFQMVDLLLTNFHAPRSTVLMLTAACAGTDNLRAA
YSEAVLQGYNFLSYGDSMLIC

Sequences:

>Translated_341_residues
MQVADFDYHLPEERIAKYPPLERGSTRLLVLNRQSGALTHSLYAHLDTFLQAGDLLLLNNTRVVPARLFATRATGATIEL
MLLERHHHREQLVLYRGQLKAGEMLQSHGHTLLVEEVLPQGLARLALADGGDLHQFFTQFGSVPIPPYLKRNAEAVDRER
YQTVFAAHSGSVAAPTASLNMTPELLQRLKNNGVEIAHITLHVGLGTFLPIRTESLEEHVMHRESYLLPAESVAALQRVK
ADGGRVVAVGTTVTRALEHSAPRILQSSAHAEIEGEADIFIYPGYRFQMVDLLLTNFHAPRSTVLMLTAACAGTDNLRAA
YSEAVLQGYNFLSYGDSMLIC
>Mature_341_residues
MQVADFDYHLPEERIAKYPPLERGSTRLLVLNRQSGALTHSLYAHLDTFLQAGDLLLLNNTRVVPARLFATRATGATIEL
MLLERHHHREQLVLYRGQLKAGEMLQSHGHTLLVEEVLPQGLARLALADGGDLHQFFTQFGSVPIPPYLKRNAEAVDRER
YQTVFAAHSGSVAAPTASLNMTPELLQRLKNNGVEIAHITLHVGLGTFLPIRTESLEEHVMHRESYLLPAESVAALQRVK
ADGGRVVAVGTTVTRALEHSAPRILQSSAHAEIEGEADIFIYPGYRFQMVDLLLTNFHAPRSTVLMLTAACAGTDNLRAA
YSEAVLQGYNFLSYGDSMLIC

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=349, Percent_Identity=42.9799426934097, Blast_Score=268, Evalue=5e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_CHLCH (Q3AR26)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_379592.1
- ProteinModelPortal:   Q3AR26
- SMR:   Q3AR26
- STRING:   Q3AR26
- GeneID:   3747437
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_1288
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   CLSK637367
- BioCyc:   CCHL340177:CAG_1288-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 37691; Mature: 37691

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVADFDYHLPEERIAKYPPLERGSTRLLVLNRQSGALTHSLYAHLDTFLQAGDLLLLNN
CCCCCCCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEC
TRVVPARLFATRATGATIELMLLERHHHREQLVLYRGQLKAGEMLQSHGHTLLVEEVLPQ
CEEEHHHHEEECCCCCEEEEEEEHHHCCHHEEEEEECCCCHHHHHHHCCCEEEHHHHHHH
GLARLALADGGDLHQFFTQFGSVPIPPYLKRNAEAVDRERYQTVFAAHSGSVAAPTASLN
HHHHHHHCCCCHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHEEECCCCCCCCCCCCC
MTPELLQRLKNNGVEIAHITLHVGLGTFLPIRTESLEEHVMHRESYLLPAESVAALQRVK
CCHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ADGGRVVAVGTTVTRALEHSAPRILQSSAHAEIEGEADIFIYPGYRFQMVDLLLTNFHAP
CCCCEEEEECHHHHHHHHHCCHHHHHCCCCCEECCCCCEEEECCCHHHHHHHHHHCCCCC
RSTVLMLTAACAGTDNLRAAYSEAVLQGYNFLSYGDSMLIC
CHHEEEEEEHHCCCCHHHHHHHHHHHHCCCHHHCCCCEEEC
>Mature Secondary Structure
MQVADFDYHLPEERIAKYPPLERGSTRLLVLNRQSGALTHSLYAHLDTFLQAGDLLLLNN
CCCCCCCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEEEC
TRVVPARLFATRATGATIELMLLERHHHREQLVLYRGQLKAGEMLQSHGHTLLVEEVLPQ
CEEEHHHHEEECCCCCEEEEEEEHHHCCHHEEEEEECCCCHHHHHHHCCCEEEHHHHHHH
GLARLALADGGDLHQFFTQFGSVPIPPYLKRNAEAVDRERYQTVFAAHSGSVAAPTASLN
HHHHHHHCCCCHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHEEECCCCCCCCCCCCC
MTPELLQRLKNNGVEIAHITLHVGLGTFLPIRTESLEEHVMHRESYLLPAESVAALQRVK
CCHHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ADGGRVVAVGTTVTRALEHSAPRILQSSAHAEIEGEADIFIYPGYRFQMVDLLLTNFHAP
CCCCEEEEECHHHHHHHHHCCHHHHHCCCCCEECCCCCEEEECCCHHHHHHHHHHCCCCC
RSTVLMLTAACAGTDNLRAAYSEAVLQGYNFLSYGDSMLIC
CHHEEEEEEHHCCCCHHHHHHHHHHHHCCCHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA