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The map label for this gene is acnB [H]

Identifier: 78189252

GI number: 78189252

Start: 1749962

End: 1752529

Strand: Reverse

Name: acnB [H]

Synonym: Cag_1286

Alternate gene names: 78189252

Gene position: 1752529-1749962 (Counterclockwise)

Preceding gene: 78189254

Following gene: 78189251

Centisome position: 68.14

GC content: 47.08

Gene sequence:

>2568_bases
ATGAGTTTGATTGAGAGCTATCGCCAGCATACAGCCGAGCGTGCTACACTTGATATTCCACCACTTCCCTTAACAGCCGA
ACAAACTCGTGAGCTTGTTGTGTTGCTCAAGCAAGAGTCGGTGCCTGAGCAAGCCTATTTGCTTGATTTGTTTTGCAACC
ATGTGAGCCCAGGTGTAGATGATGCGGCTTTTGAAAAAGCGGTATTTCTTGATGCTATTGTAAAAGGTGAAGTAGCCTGC
AACGCTATTTCAGCCGTTGAAGCAGTGCGTATTCTTGGTACCATGCTTGGTGGTTACAATGTGAAGCCATTGGTTGATGC
GCTAAGCCATAGCGATACGGCTATTTGTAGCGAAGCTGCTGCCATGCTTAAAAAGACATTGTTGGTGTATGATCTTTTTG
AGGTGGTGGTTGAAATGGCAAAAAGCAATAAATATGCGGCTGAAGTGGTGAACTCATGGGCGGAAGCTGAGTGGTTTACC
TCACGTCCAGCATTGCCCGAAAAAATGACCCTCACCGTCTTTAAAGTTCCGGGTGAAACCAACACCGATGATCTCTCCCC
AGCAAGTGAAGCCTTTACTCGCAGCGATATTCCGCTTCATGCGCGTTGTATGCTTGGCACAAAAATTAATGATCCAATTG
GTACCATTGCAGCCTTAAAGCAAAAAGGGCATCCGTTAGCGTATGTTGGCGATGTTGTTGGCACAGGCTCCAGCCGTAAA
TCAGGCATAAACTCGGTGCAGTGGCACATTGGTAGCGATATTCCAGCCGTACCAAACAAGCGAACGGGCGGCGTTGTACT
TGGTGGCATTATTGCTCCAATTTTCTTTAACACGGCTGAAGATTCAGGTGCACTGCCAATTCAAGCTGATGTTAATGCAC
TTGAAATGGGCGATGTTATTGAAGTAAATCTTATTGAAGGCACCATTTCTAAAGATGGAGCCGTTGTTTCACGTTTTGAA
CTATCACCAAATACCATTGGCGACGAAGTTCGTGCAGGTGGACGTATTCCGTTGATTATTGGTCGCACCTTAACACGCAA
AGCTCGCACTGCGCTTGGTATGGGTGACGATACGCTTTTTGTGCGTCCTGAGCAGCCAGCCGATAGCGGTAAAGGTTACA
CGCTTGCGCAGAAAATGATTGGTAAAGCATGTGGCTTAGCTGGTGTTCGTCCCGGCATGTACGTTGAACCAGCAACCTTA
ACCGTTGGTTCGCAAGATACCACTGGTGCTATGACGCGCGATGAAGTAAAAGAGCTTGCGGCGCTTAGCTTTTCGGCTGA
TCTCTTTATGCAGAGCTTCTGCCACACCTCAGCCTATCCAAAACCATCCGACATTAAGCTTCACAGAAGCTTGCCATACT
TTATTATGAGTCGTGGTGGTGTATCGCTGAAGCCGGGTGATGGTGTTATTCACACATGGTTAAACCGCATGGTGTTGCCT
GATGCACTTGGCACGGGTGGTGACTCACACACTCGTTTCCCAATCGGCGTATCGTTCCCTGCCGGTTCTGGTCTTGTTGC
TTTTGCTGCTGTAACGGGTGCTATGCCGTTGAACGTGCCTGAGTCAGTGCTTGTGCGCTTTACCGGTGAATTACAAGCAG
GTATTACCTTGCGCGATCTCGTTAATGCAATTCCACACGAAGCTATTAAGCGTGGGTTGTTAACGGTTGAGAAAAAAGGC
AAGAAAAATATTTTTGCAGGTCGCATTCTTGAAATTGAAGGGTTACCCAACCTTAAAGTTGAGCAAGCTTTTGAATTAAG
CGATGCTTCAGCCGAGCGTAGTGCGGCAGCTTGTACCGTTCGTTTAAACAAAGAGCCTGTTATTGAATACCTCAGCTCAA
ACGTCAAATTGCTTGAGCAAATGATTGAAGAGGGGTATGGCAACTCCGACACCATTCAACGCCGTATTGATAAAATGCAA
GAGTGGCTAAAGAATCCAATCTTGCTTGAGCCTGATGCTGACGCAGAATATGCGGCTGTGGTTGAAATTGATATGAGTAC
CATTACCGAGCCAATTCTTGCCTGCCCAAATGACCCCGATGATGTTATGACGCTGAGCGAAGTGCTCGCTGATGCAAAAC
GTCCAAAAACCATCAACGAAGTATTTGTTGGAAGCTGCATGACCAACATTGGGCACTTCCGTGCACTTGGTGAAATTTTA
CGTGGCAAAGGTGCTGTACCAACTCGTCTTTGGATTGTACCGCCAACTAAAATGGATATGAACAAGCTCATTGAAGAGGG
CTACTACTCCGTTTTTGGTGCGGCTGGTGCACGTACCGAACAACCCGGCTGTTCGCTTTGCATGGGTAACCAAGCGCGTG
TAGCTGACAATGTGGTGGTATTTTCAACCAGCACACGCAACTTCGACAATCGCATGGGTACAGGCGCACAAGTTTATCTT
GGTTCTGCGGAGCTTGCTGCGGTGTGCGCTTTACTTGGTCGTATGCCAAACAAAGAGGAGTACATGGAGGTTGTTTCAGG
CACTCTTACAGCAAAAAGTTCAGCCGTTTATGAGTATCTCAATTTCCACGATTTGAAAAAAGCCGATTTAGAGCTACTCG
TTCACTAA

Upstream 100 bases:

>100_bases
GCTCCTTTTGCTTCTGTGAGCAATGCAAATCCTGCATTTTTAGGTAATCATTTTTTCTACAACAAACATATCCATTAAGT
AACTGAGGGAGATCTGCCTT

Downstream 100 bases:

>100_bases
TATCGCGTAGATTCAAGGCGGAACACCGCGCTAAAATTTTATGTGTTCCGCCTGAAAATTGGTTTTTGAGATATCGTTCC
ATTTGTTTATCATAAGCAAG

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 855; Mature: 854

Protein sequence:

>855_residues
MSLIESYRQHTAERATLDIPPLPLTAEQTRELVVLLKQESVPEQAYLLDLFCNHVSPGVDDAAFEKAVFLDAIVKGEVAC
NAISAVEAVRILGTMLGGYNVKPLVDALSHSDTAICSEAAAMLKKTLLVYDLFEVVVEMAKSNKYAAEVVNSWAEAEWFT
SRPALPEKMTLTVFKVPGETNTDDLSPASEAFTRSDIPLHARCMLGTKINDPIGTIAALKQKGHPLAYVGDVVGTGSSRK
SGINSVQWHIGSDIPAVPNKRTGGVVLGGIIAPIFFNTAEDSGALPIQADVNALEMGDVIEVNLIEGTISKDGAVVSRFE
LSPNTIGDEVRAGGRIPLIIGRTLTRKARTALGMGDDTLFVRPEQPADSGKGYTLAQKMIGKACGLAGVRPGMYVEPATL
TVGSQDTTGAMTRDEVKELAALSFSADLFMQSFCHTSAYPKPSDIKLHRSLPYFIMSRGGVSLKPGDGVIHTWLNRMVLP
DALGTGGDSHTRFPIGVSFPAGSGLVAFAAVTGAMPLNVPESVLVRFTGELQAGITLRDLVNAIPHEAIKRGLLTVEKKG
KKNIFAGRILEIEGLPNLKVEQAFELSDASAERSAAACTVRLNKEPVIEYLSSNVKLLEQMIEEGYGNSDTIQRRIDKMQ
EWLKNPILLEPDADAEYAAVVEIDMSTITEPILACPNDPDDVMTLSEVLADAKRPKTINEVFVGSCMTNIGHFRALGEIL
RGKGAVPTRLWIVPPTKMDMNKLIEEGYYSVFGAAGARTEQPGCSLCMGNQARVADNVVVFSTSTRNFDNRMGTGAQVYL
GSAELAAVCALLGRMPNKEEYMEVVSGTLTAKSSAVYEYLNFHDLKKADLELLVH

Sequences:

>Translated_855_residues
MSLIESYRQHTAERATLDIPPLPLTAEQTRELVVLLKQESVPEQAYLLDLFCNHVSPGVDDAAFEKAVFLDAIVKGEVAC
NAISAVEAVRILGTMLGGYNVKPLVDALSHSDTAICSEAAAMLKKTLLVYDLFEVVVEMAKSNKYAAEVVNSWAEAEWFT
SRPALPEKMTLTVFKVPGETNTDDLSPASEAFTRSDIPLHARCMLGTKINDPIGTIAALKQKGHPLAYVGDVVGTGSSRK
SGINSVQWHIGSDIPAVPNKRTGGVVLGGIIAPIFFNTAEDSGALPIQADVNALEMGDVIEVNLIEGTISKDGAVVSRFE
LSPNTIGDEVRAGGRIPLIIGRTLTRKARTALGMGDDTLFVRPEQPADSGKGYTLAQKMIGKACGLAGVRPGMYVEPATL
TVGSQDTTGAMTRDEVKELAALSFSADLFMQSFCHTSAYPKPSDIKLHRSLPYFIMSRGGVSLKPGDGVIHTWLNRMVLP
DALGTGGDSHTRFPIGVSFPAGSGLVAFAAVTGAMPLNVPESVLVRFTGELQAGITLRDLVNAIPHEAIKRGLLTVEKKG
KKNIFAGRILEIEGLPNLKVEQAFELSDASAERSAAACTVRLNKEPVIEYLSSNVKLLEQMIEEGYGNSDTIQRRIDKMQ
EWLKNPILLEPDADAEYAAVVEIDMSTITEPILACPNDPDDVMTLSEVLADAKRPKTINEVFVGSCMTNIGHFRALGEIL
RGKGAVPTRLWIVPPTKMDMNKLIEEGYYSVFGAAGARTEQPGCSLCMGNQARVADNVVVFSTSTRNFDNRMGTGAQVYL
GSAELAAVCALLGRMPNKEEYMEVVSGTLTAKSSAVYEYLNFHDLKKADLELLVH
>Mature_854_residues
SLIESYRQHTAERATLDIPPLPLTAEQTRELVVLLKQESVPEQAYLLDLFCNHVSPGVDDAAFEKAVFLDAIVKGEVACN
AISAVEAVRILGTMLGGYNVKPLVDALSHSDTAICSEAAAMLKKTLLVYDLFEVVVEMAKSNKYAAEVVNSWAEAEWFTS
RPALPEKMTLTVFKVPGETNTDDLSPASEAFTRSDIPLHARCMLGTKINDPIGTIAALKQKGHPLAYVGDVVGTGSSRKS
GINSVQWHIGSDIPAVPNKRTGGVVLGGIIAPIFFNTAEDSGALPIQADVNALEMGDVIEVNLIEGTISKDGAVVSRFEL
SPNTIGDEVRAGGRIPLIIGRTLTRKARTALGMGDDTLFVRPEQPADSGKGYTLAQKMIGKACGLAGVRPGMYVEPATLT
VGSQDTTGAMTRDEVKELAALSFSADLFMQSFCHTSAYPKPSDIKLHRSLPYFIMSRGGVSLKPGDGVIHTWLNRMVLPD
ALGTGGDSHTRFPIGVSFPAGSGLVAFAAVTGAMPLNVPESVLVRFTGELQAGITLRDLVNAIPHEAIKRGLLTVEKKGK
KNIFAGRILEIEGLPNLKVEQAFELSDASAERSAAACTVRLNKEPVIEYLSSNVKLLEQMIEEGYGNSDTIQRRIDKMQE
WLKNPILLEPDADAEYAAVVEIDMSTITEPILACPNDPDDVMTLSEVLADAKRPKTINEVFVGSCMTNIGHFRALGEILR
GKGAVPTRLWIVPPTKMDMNKLIEEGYYSVFGAAGARTEQPGCSLCMGNQARVADNVVVFSTSTRNFDNRMGTGAQVYLG
SAELAAVCALLGRMPNKEEYMEVVSGTLTAKSSAVYEYLNFHDLKKADLELLVH

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=859, Percent_Identity=62.8637951105937, Blast_Score=1039, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 92045; Mature: 91914

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLIESYRQHTAERATLDIPPLPLTAEQTRELVVLLKQESVPEQAYLLDLFCNHVSPGVD
CCHHHHHHHHHHHCCEECCCCCCCCHHHHHHEEEEECCCCCCHHHHHHHHHHHHCCCCCC
DAAFEKAVFLDAIVKGEVACNAISAVEAVRILGTMLGGYNVKPLVDALSHSDTAICSEAA
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHH
AMLKKTLLVYDLFEVVVEMAKSNKYAAEVVNSWAEAEWFTSRPALPEKMTLTVFKVPGET
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCC
NTDDLSPASEAFTRSDIPLHARCMLGTKINDPIGTIAALKQKGHPLAYVGDVVGTGSSRK
CCCCCCCHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCEEEECHHHCCCCCCC
SGINSVQWHIGSDIPAVPNKRTGGVVLGGIIAPIFFNTAEDSGALPIQADVNALEMGDVI
CCCCCEEEECCCCCCCCCCCCCCCEEEHHHHHHHHEECCCCCCCCEEECCCCEEECCCEE
EVNLIEGTISKDGAVVSRFELSPNTIGDEVRAGGRIPLIIGRTLTRKARTALGMGDDTLF
EEEEEECCCCCCCCEEEEEECCCCCCCHHHHCCCCEEEEECCHHHHHHHHHCCCCCCEEE
VRPEQPADSGKGYTLAQKMIGKACGLAGVRPGMYVEPATLTVGSQDTTGAMTRDEVKELA
ECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEECCCEEEECCCCCCCCCCHHHHHHHH
ALSFSADLFMQSFCHTSAYPKPSDIKLHRSLPYFIMSRGGVSLKPGDGVIHTWLNRMVLP
HHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEECCCCEECCCCCHHHHHHHHHCCC
DALGTGGDSHTRFPIGVSFPAGSGLVAFAAVTGAMPLNVPESVLVRFTGELQAGITLRDL
HHCCCCCCCCCEEEEEEECCCCCCCEEHHHHHCCCCCCCCHHHHEEECCCHHCCEEHHHH
VNAIPHEAIKRGLLTVEKKGKKNIFAGRILEIEGLPNLKVEQAFELSDASAERSAAACTV
HHHCCHHHHHHCCEEEECCCCCCEEECEEEEECCCCCCCHHHHHCCCCCCCCCCCEEEEE
RLNKEPVIEYLSSNVKLLEQMIEEGYGNSDTIQRRIDKMQEWLKNPILLEPDADAEYAAV
EECCHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCEEEE
VEIDMSTITEPILACPNDPDDVMTLSEVLADAKRPKTINEVFVGSCMTNIGHFRALGEIL
EEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RGKGAVPTRLWIVPPTKMDMNKLIEEGYYSVFGAAGARTEQPGCSLCMGNQARVADNVVV
HCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCEEECCEEE
FSTSTRNFDNRMGTGAQVYLGSAELAAVCALLGRMPNKEEYMEVVSGTLTAKSSAVYEYL
EECCCCCCHHHCCCCCEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHH
NFHDLKKADLELLVH
HHHHCCCCCHHEECC
>Mature Secondary Structure 
SLIESYRQHTAERATLDIPPLPLTAEQTRELVVLLKQESVPEQAYLLDLFCNHVSPGVD
CHHHHHHHHHHHCCEECCCCCCCCHHHHHHEEEEECCCCCCHHHHHHHHHHHHCCCCCC
DAAFEKAVFLDAIVKGEVACNAISAVEAVRILGTMLGGYNVKPLVDALSHSDTAICSEAA
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHH
AMLKKTLLVYDLFEVVVEMAKSNKYAAEVVNSWAEAEWFTSRPALPEKMTLTVFKVPGET
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCC
NTDDLSPASEAFTRSDIPLHARCMLGTKINDPIGTIAALKQKGHPLAYVGDVVGTGSSRK
CCCCCCCHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCEEEECHHHCCCCCCC
SGINSVQWHIGSDIPAVPNKRTGGVVLGGIIAPIFFNTAEDSGALPIQADVNALEMGDVI
CCCCCEEEECCCCCCCCCCCCCCCEEEHHHHHHHHEECCCCCCCCEEECCCCEEECCCEE
EVNLIEGTISKDGAVVSRFELSPNTIGDEVRAGGRIPLIIGRTLTRKARTALGMGDDTLF
EEEEEECCCCCCCCEEEEEECCCCCCCHHHHCCCCEEEEECCHHHHHHHHHCCCCCCEEE
VRPEQPADSGKGYTLAQKMIGKACGLAGVRPGMYVEPATLTVGSQDTTGAMTRDEVKELA
ECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEECCCEEEECCCCCCCCCCHHHHHHHH
ALSFSADLFMQSFCHTSAYPKPSDIKLHRSLPYFIMSRGGVSLKPGDGVIHTWLNRMVLP
HHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEECCCCEECCCCCHHHHHHHHHCCC
DALGTGGDSHTRFPIGVSFPAGSGLVAFAAVTGAMPLNVPESVLVRFTGELQAGITLRDL
HHCCCCCCCCCEEEEEEECCCCCCCEEHHHHHCCCCCCCCHHHHEEECCCHHCCEEHHHH
VNAIPHEAIKRGLLTVEKKGKKNIFAGRILEIEGLPNLKVEQAFELSDASAERSAAACTV
HHHCCHHHHHHCCEEEECCCCCCEEECEEEEECCCCCCCHHHHHCCCCCCCCCCCEEEEE
RLNKEPVIEYLSSNVKLLEQMIEEGYGNSDTIQRRIDKMQEWLKNPILLEPDADAEYAAV
EECCHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCEEEE
VEIDMSTITEPILACPNDPDDVMTLSEVLADAKRPKTINEVFVGSCMTNIGHFRALGEIL
EEEEHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RGKGAVPTRLWIVPPTKMDMNKLIEEGYYSVFGAAGARTEQPGCSLCMGNQARVADNVVV
HCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCEEECCEEE
FSTSTRNFDNRMGTGAQVYLGSAELAAVCALLGRMPNKEEYMEVVSGTLTAKSSAVYEYL
EECCCCCCHHHCCCCCEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHHH
NFHDLKKADLELLVH
HHHHCCCCCHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]