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The map label for this gene is capI [H]

Identifier: 78189149

GI number: 78189149

Start: 1575023

End: 1576036

Strand: Reverse

Name: capI [H]

Synonym: Cag_1182

Alternate gene names: 78189149

Gene position: 1576036-1575023 (Counterclockwise)

Preceding gene: 78189154

Following gene: 78189141

Centisome position: 61.27

GC content: 46.84

Gene sequence:

>1014_bases
ATGAATGTGTTAGTTACAGGCGCTGCTGGCTTTATTGGCTCTACTCTTTGCAAGCGCTTGCTGGAACGCGGCGATCGCGT
AACGGGTATTGATAACCTTAACGATTATTACGATGTCTCCTTAAAAGAGGCTCGTTTAGCGCAGCTTCAGCCATATGAAA
ATTTTACCTTTGTAAAGGGAGATTTAGCCGATCGCGCTGGTATGGAGGCACTTTTTGCAAAGGGTGAATTTGAGGGCGTA
GTCAACTTGGCAGCACAAGCGGGGGTGCGCTATTCCATTGAAAATCCTCATTCTTATGTAGAAAGCAATATTGTTGGATT
TTTGCACATTCTTGAAGGATGTCGCCATCACGGCGTGAAGCACTTGGTTTATGCCTCATCCAGCTCAGTGTATGGCGCAA
ACGAAACCATGCCATTTTCGGTACACGACAACGTTGACCATCCACTTTCGCTTTACGCTGCCAGCAAAAAAGCCAATGAA
TTAATGGCGCATACCTATAGCCACCTTTACAACATTCCAACTACGGGCTTGCGCTTTTTCACCGTCTATGGACCGTGGGG
GCGCCCCGATATGGCGCTCTTTTTGTTTACCGATGCTATTCTAAAAAACAAGCCCATCAAGGTTTTTAACTACGGCAAGC
ACCGTCGCGACTTCACTTACATTGATGATATTGTGGAAGGCGTTATTCGCACGCTTGACCACACAGCAACGCCAAATCCA
GCATGGAGTGGGGCAACACCCGATCCGGGCAGCAGCAAAGCGCCATGGAGAGTTTATAATATTGGCAATAGCCAACCAGT
GGAGTTGATGGATTACATTCAAGCGCTGGAAAACGAGCTTGGTCGGACGGCAATTAAGGAGTTTTTGCCGCTTCAGCCTG
GTGATGTACCCGATACCTATGCCGATGTTGATCAGCTTATTGAAGATGTGCACTACAAACCGCAAACGAGCGTGCCAGAA
GGGGTAAAACGTTTTGTTGCTTGGTATAAAGAATATTATGGAGTAAAAGGGTAA

Upstream 100 bases:

>100_bases
AGCCAACAGCTCTTTTGAATTTCTTTGTTCACTCTCATTGAATTGCTTTTATTGAAACGATTTTAAAAGATTTTCCTCTA
ACCTCTCAATAATTAATAGC

Downstream 100 bases:

>100_bases
GAGAGGAAAATATGGAAAAATGGGGTACTTATTGCAATACCCCATTTTTCCATAATTCATAGCCGCTTACATCCATCCCG
TCATTCCATGCAATGCCATA

Product: capsular polysaccharide biosynthesis protein I

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 337; Mature: 337

Protein sequence:

>337_residues
MNVLVTGAAGFIGSTLCKRLLERGDRVTGIDNLNDYYDVSLKEARLAQLQPYENFTFVKGDLADRAGMEALFAKGEFEGV
VNLAAQAGVRYSIENPHSYVESNIVGFLHILEGCRHHGVKHLVYASSSSVYGANETMPFSVHDNVDHPLSLYAASKKANE
LMAHTYSHLYNIPTTGLRFFTVYGPWGRPDMALFLFTDAILKNKPIKVFNYGKHRRDFTYIDDIVEGVIRTLDHTATPNP
AWSGATPDPGSSKAPWRVYNIGNSQPVELMDYIQALENELGRTAIKEFLPLQPGDVPDTYADVDQLIEDVHYKPQTSVPE
GVKRFVAWYKEYYGVKG

Sequences:

>Translated_337_residues
MNVLVTGAAGFIGSTLCKRLLERGDRVTGIDNLNDYYDVSLKEARLAQLQPYENFTFVKGDLADRAGMEALFAKGEFEGV
VNLAAQAGVRYSIENPHSYVESNIVGFLHILEGCRHHGVKHLVYASSSSVYGANETMPFSVHDNVDHPLSLYAASKKANE
LMAHTYSHLYNIPTTGLRFFTVYGPWGRPDMALFLFTDAILKNKPIKVFNYGKHRRDFTYIDDIVEGVIRTLDHTATPNP
AWSGATPDPGSSKAPWRVYNIGNSQPVELMDYIQALENELGRTAIKEFLPLQPGDVPDTYADVDQLIEDVHYKPQTSVPE
GVKRFVAWYKEYYGVKG
>Mature_337_residues
MNVLVTGAAGFIGSTLCKRLLERGDRVTGIDNLNDYYDVSLKEARLAQLQPYENFTFVKGDLADRAGMEALFAKGEFEGV
VNLAAQAGVRYSIENPHSYVESNIVGFLHILEGCRHHGVKHLVYASSSSVYGANETMPFSVHDNVDHPLSLYAASKKANE
LMAHTYSHLYNIPTTGLRFFTVYGPWGRPDMALFLFTDAILKNKPIKVFNYGKHRRDFTYIDDIVEGVIRTLDHTATPNP
AWSGATPDPGSSKAPWRVYNIGNSQPVELMDYIQALENELGRTAIKEFLPLQPGDVPDTYADVDQLIEDVHYKPQTSVPE
GVKRFVAWYKEYYGVKG

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=341, Percent_Identity=26.099706744868, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI56237023, Length=351, Percent_Identity=25.6410256410256, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI56118217, Length=351, Percent_Identity=25.6410256410256, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI189083684, Length=351, Percent_Identity=25.6410256410256, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI42516563, Length=335, Percent_Identity=23.5820895522388, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1788353, Length=356, Percent_Identity=25.561797752809, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI48994969, Length=358, Percent_Identity=26.8156424581006, Blast_Score=108, Evalue=7e-25,
Organism=Escherichia coli, GI1786974, Length=351, Percent_Identity=24.2165242165242, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1790049, Length=338, Percent_Identity=24.2603550295858, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71982035, Length=359, Percent_Identity=25.6267409470752, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71982038, Length=361, Percent_Identity=25.4847645429363, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17568069, Length=236, Percent_Identity=28.3898305084746, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17539532, Length=335, Percent_Identity=24.1791044776119, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI115532424, Length=349, Percent_Identity=23.7822349570201, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=355, Percent_Identity=25.9154929577465, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19923002, Length=356, Percent_Identity=25.561797752809, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356223, Length=334, Percent_Identity=22.1556886227545, Blast_Score=91, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 37677; Mature: 37677

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLVTGAAGFIGSTLCKRLLERGDRVTGIDNLNDYYDVSLKEARLAQLQPYENFTFVKG
CCEEEECCHHHHHHHHHHHHHHCCCCEECCCCCCCHHCCCHHHHHHHHCCCCCCCEEEEC
DLADRAGMEALFAKGEFEGVVNLAAQAGVRYSIENPHSYVESNIVGFLHILEGCRHHGVK
CHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCE
HLVYASSSSVYGANETMPFSVHDNVDHPLSLYAASKKANELMAHTYSHLYNIPTTGLRFF
EEEEECCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
TVYGPWGRPDMALFLFTDAILKNKPIKVFNYGKHRRDFTYIDDIVEGVIRTLDHTATPNP
EEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
AWSGATPDPGSSKAPWRVYNIGNSQPVELMDYIQALENELGRTAIKEFLPLQPGDVPDTY
CCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
ADVDQLIEDVHYKPQTSVPEGVKRFVAWYKEYYGVKG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNVLVTGAAGFIGSTLCKRLLERGDRVTGIDNLNDYYDVSLKEARLAQLQPYENFTFVKG
CCEEEECCHHHHHHHHHHHHHHCCCCEECCCCCCCHHCCCHHHHHHHHCCCCCCCEEEEC
DLADRAGMEALFAKGEFEGVVNLAAQAGVRYSIENPHSYVESNIVGFLHILEGCRHHGVK
CHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCE
HLVYASSSSVYGANETMPFSVHDNVDHPLSLYAASKKANELMAHTYSHLYNIPTTGLRFF
EEEEECCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
TVYGPWGRPDMALFLFTDAILKNKPIKVFNYGKHRRDFTYIDDIVEGVIRTLDHTATPNP
EEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCC
AWSGATPDPGSSKAPWRVYNIGNSQPVELMDYIQALENELGRTAIKEFLPLQPGDVPDTY
CCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
ADVDQLIEDVHYKPQTSVPEGVKRFVAWYKEYYGVKG
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]