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The map label for this gene is ddl

Identifier: 78188875

GI number: 78188875

Start: 1239058

End: 1240140

Strand: Reverse

Name: ddl

Synonym: Cag_0905

Alternate gene names: 78188875

Gene position: 1240140-1239058 (Counterclockwise)

Preceding gene: 78188885

Following gene: 78188874

Centisome position: 48.22

GC content: 51.34

Gene sequence:

>1083_bases
ATGTCTCGAACTACTGTCGCCCTCTTTTTTGGCGGTCAATCCGCTGAGCATGAAATTTCTATTATTTCCGCCCAATCTAT
TGCCGCACACCTCGACACGGAGCGCTTTACACTGCTCCCTATTTACATAACTCATTCGGGTGAATGGCTTTGCGATGGCT
TTGCCCGCACCTTGCTCACCACCAATCTTGCAAGTAAGCTACGTGGCTCATCGCGCGAAGAGACGGCAGCGGCGTTACAG
CAAATGGTGCGCAATGCGGCTCAAGCGCCCTGCAATCGCAACCTTGCAGCGCTTGGCGTGGATGTGGCATTTTTAGCCTT
ACATGGCAGCTTTGGTGAGGATGGTAGAATGCAGGGTTTTCTTGAAACGTGTGGCATTCCCTACACGGGATGCGGAGTAC
TTGCTTCGGCACTAACGATGGATAAAGCGCTCACCAAGCTCTGCGTTGCCGATGCGGGCATTGCAGTTGCTCAAGGTACC
AACATTTTAAGCGCCGACTACCTCGCGAACCCCAACGCCGTAGAAGCCTCTGTTGAAGCGCAAGTAAGCTATCCGCTCTT
TGTAAAGCCCGCAAGCCTTGGCTCCTCCATTGGCATTAGTAAAGTACATAATCGCGAAGAGCTGCACCCCGCTCTGCAAG
CAGCCTGTGCCCTTGATTGGAAGGTAGTGGTTGAAAGCACGGTTAAAGGGCGCGAAATTGAAGTTGCCGTGCTTGGCAAT
GCTGACCCGATTGCTTCTGTATGTGGTGAAATTGAACCAGGCAAAGAGTTTTACGATTTTCAGGATAAGTACATGGGCAA
CAGCGCCAAACTCTTTATTCCCGCACGCATTCCCGAAAGCTTGCAAGAGGAGGTTCGCCGCTCGGCATTAACGGCATATC
GCGCACTTGGGTGCAGCGGCATGGCTCGGGTTGATTTTTTTGTAGATGAATCAACCAACTCGGTGGTACTTAACGAGGTA
AACACGATTCCGGGCTTTACCGATATTAGCATGTACCCGCAAATGATGGAAGCCTCAGGCATTAGCTACCGCAACCTTAT
TACCCGCTTGCTTGAACTTGCTCTTGAACCCTTGAGACGCTAA

Upstream 100 bases:

>100_bases
GCGGCATTTCTACAGAGCTACAAAAAAGTGTAGGGAAATATGGCGCTTGCAGGTATTTTCCACCCCAAAAGCATATTAGC
TCACCTCTTTAACCATTCCC

Downstream 100 bases:

>100_bases
TGGCTGAAACCGATACACCATACCTACGCTTTGCTGATATTTCGCTCGATATTTCGCGTGGCAACTATAGCGCCGCTCGC
CAAAAGCTTGAGGTGCTTGA

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MSRTTVALFFGGQSAEHEISIISAQSIAAHLDTERFTLLPIYITHSGEWLCDGFARTLLTTNLASKLRGSSREETAAALQ
QMVRNAAQAPCNRNLAALGVDVAFLALHGSFGEDGRMQGFLETCGIPYTGCGVLASALTMDKALTKLCVADAGIAVAQGT
NILSADYLANPNAVEASVEAQVSYPLFVKPASLGSSIGISKVHNREELHPALQAACALDWKVVVESTVKGREIEVAVLGN
ADPIASVCGEIEPGKEFYDFQDKYMGNSAKLFIPARIPESLQEEVRRSALTAYRALGCSGMARVDFFVDESTNSVVLNEV
NTIPGFTDISMYPQMMEASGISYRNLITRLLELALEPLRR

Sequences:

>Translated_360_residues
MSRTTVALFFGGQSAEHEISIISAQSIAAHLDTERFTLLPIYITHSGEWLCDGFARTLLTTNLASKLRGSSREETAAALQ
QMVRNAAQAPCNRNLAALGVDVAFLALHGSFGEDGRMQGFLETCGIPYTGCGVLASALTMDKALTKLCVADAGIAVAQGT
NILSADYLANPNAVEASVEAQVSYPLFVKPASLGSSIGISKVHNREELHPALQAACALDWKVVVESTVKGREIEVAVLGN
ADPIASVCGEIEPGKEFYDFQDKYMGNSAKLFIPARIPESLQEEVRRSALTAYRALGCSGMARVDFFVDESTNSVVLNEV
NTIPGFTDISMYPQMMEASGISYRNLITRLLELALEPLRR
>Mature_359_residues
SRTTVALFFGGQSAEHEISIISAQSIAAHLDTERFTLLPIYITHSGEWLCDGFARTLLTTNLASKLRGSSREETAAALQQ
MVRNAAQAPCNRNLAALGVDVAFLALHGSFGEDGRMQGFLETCGIPYTGCGVLASALTMDKALTKLCVADAGIAVAQGTN
ILSADYLANPNAVEASVEAQVSYPLFVKPASLGSSIGISKVHNREELHPALQAACALDWKVVVESTVKGREIEVAVLGNA
DPIASVCGEIEPGKEFYDFQDKYMGNSAKLFIPARIPESLQEEVRRSALTAYRALGCSGMARVDFFVDESTNSVVLNEVN
TIPGFTDISMYPQMMEASGISYRNLITRLLELALEPLRR

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=359, Percent_Identity=43.1754874651811, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI1786280, Length=261, Percent_Identity=37.9310344827586, Blast_Score=176, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_CHLCH (Q3AS55)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_379213.1
- ProteinModelPortal:   Q3AS55
- SMR:   Q3AS55
- STRING:   Q3AS55
- GeneID:   3748096
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_0905
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- ProtClustDB:   PRK01966
- BioCyc:   CCHL340177:CAG_0905-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 38732; Mature: 38600

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTTVALFFGGQSAEHEISIISAQSIAAHLDTERFTLLPIYITHSGEWLCDGFARTLLT
CCCEEEEEEECCCCCCCEEEEEEHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHH
TNLASKLRGSSREETAAALQQMVRNAAQAPCNRNLAALGVDVAFLALHGSFGEDGRMQGF
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEEEEEECCCCCCCCHHHH
LETCGIPYTGCGVLASALTMDKALTKLCVADAGIAVAQGTNILSADYLANPNAVEASVEA
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEHHHCCCCCCEEEEEEE
QVSYPLFVKPASLGSSIGISKVHNREELHPALQAACALDWKVVVESTVKGREIEVAVLGN
EECCCEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHCCHHEEEHHCCCCCEEEEEEECC
ADPIASVCGEIEPGKEFYDFQDKYMGNSAKLFIPARIPESLQEEVRRSALTAYRALGCSG
CCHHHHHHCCCCCCHHHHCCHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCC
MARVDFFVDESTNSVVLNEVNTIPGFTDISMYPQMMEASGISYRNLITRLLELALEPLRR
CEEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRTTVALFFGGQSAEHEISIISAQSIAAHLDTERFTLLPIYITHSGEWLCDGFARTLLT
CCEEEEEEECCCCCCCEEEEEEHHHHHHHCCCCCEEEEEEEEECCCCCHHHHHHHHHHH
TNLASKLRGSSREETAAALQQMVRNAAQAPCNRNLAALGVDVAFLALHGSFGEDGRMQGF
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHEEEEEEECCCCCCCCHHHH
LETCGIPYTGCGVLASALTMDKALTKLCVADAGIAVAQGTNILSADYLANPNAVEASVEA
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEHHHCCCCCCEEEEEEE
QVSYPLFVKPASLGSSIGISKVHNREELHPALQAACALDWKVVVESTVKGREIEVAVLGN
EECCCEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHCCHHEEEHHCCCCCEEEEEEECC
ADPIASVCGEIEPGKEFYDFQDKYMGNSAKLFIPARIPESLQEEVRRSALTAYRALGCSG
CCHHHHHHCCCCCCHHHHCCHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCC
MARVDFFVDESTNSVVLNEVNTIPGFTDISMYPQMMEASGISYRNLITRLLELALEPLRR
CEEEEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA