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The map label for this gene is folD

Identifier: 78188750

GI number: 78188750

Start: 1097968

End: 1098855

Strand: Reverse

Name: folD

Synonym: Cag_0779

Alternate gene names: 78188750

Gene position: 1098855-1097968 (Counterclockwise)

Preceding gene: 78188756

Following gene: 78188749

Centisome position: 42.72

GC content: 44.14

Gene sequence:

>888_bases
ATGATTGTAATTGATGGAAAAAAAGTATCGCTTGAGCTAAAAAATGAGTTGCGCGAACGTGTGGAGGCGCTGAATGCCAA
ATGTGGCAAAGTGCCCGGATTAACGGTTATTATTGTTGGCGAAGATCCTGCTTCACAAGTGTATGTGCGCAACAAAGCCA
AAAGCTGCAAAGAGATTGGCATGAACTCTACGGTGATTGAACTGCCAGCCGATACCACACAAGAGCATCTTTTAAAGATC
ATCAACGATTTAAATAATGATGATACGGTTCACGGTATTTTAGTGCAGCAGCCAATGCCCAAGCAAATTGATGAATTTAC
CATCACCCTCGCTATTGATCCCGCAAAAGATGTTGACGGCTTCCACCCCGAAAATCTTGGTCGTTTGGTTATGGGGCATC
TTGATAAATGTTTTGTAAGCTGTACACCATACGGCATTCTTGAGTTGCTTGGACGTTACAACATTGAAACCAAAGGCAAG
CATTGTGTGGTGGTTGGGCGCAGCAACATCGTCGGTAAACCAATGGCAAACCTCATGTTGCAAAAGCTGAAAGAGAGCAA
CTGCACGGTAACCATTTGCCACTCAGCAACACCAAACATTACAGAATTCACGAAGCAAGCTGATATTCTTATTGCTGCAA
TTGGTAAAGCTAATTTTATTACGGCTGATATGGTAAAAGCTGGCGCTGTGGTGATTGATGTGGGTATTAATCGCGTTGAC
GATGCTTCGGCAAAAAATGGGTACCGTTTGGTAGGCGATGTTGAGTATGCAGGTGTTTCAGCATTAGCATCAGCCATTAC
GCCTGTACCAGGTGGCGTTGGTCCTATGACTATTGCCATGCTACTTAAAAACACCGTGCAATCGTTCCAACGCGTGAACG
GACTATAA

Upstream 100 bases:

>100_bases
CAGCTTCCCTTTGCTCATTGGCTGCTGCGGGAATAAGCCATATGGTAGTTATATTCGCCCTTTCTATGTAATCCTTCTAA
CCCCTACTCTCCCTACTTCT

Downstream 100 bases:

>100_bases
AAAGGCGCGATAACTCACTATGGCAAAAGCTACTACCCGCTACGTTTGCTCCAACTGTGGCGCTGTTTCTCTTAAATATC
AAGGGAAATGTTTTGAGTGC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MIVIDGKKVSLELKNELRERVEALNAKCGKVPGLTVIIVGEDPASQVYVRNKAKSCKEIGMNSTVIELPADTTQEHLLKI
INDLNNDDTVHGILVQQPMPKQIDEFTITLAIDPAKDVDGFHPENLGRLVMGHLDKCFVSCTPYGILELLGRYNIETKGK
HCVVVGRSNIVGKPMANLMLQKLKESNCTVTICHSATPNITEFTKQADILIAAIGKANFITADMVKAGAVVIDVGINRVD
DASAKNGYRLVGDVEYAGVSALASAITPVPGGVGPMTIAMLLKNTVQSFQRVNGL

Sequences:

>Translated_295_residues
MIVIDGKKVSLELKNELRERVEALNAKCGKVPGLTVIIVGEDPASQVYVRNKAKSCKEIGMNSTVIELPADTTQEHLLKI
INDLNNDDTVHGILVQQPMPKQIDEFTITLAIDPAKDVDGFHPENLGRLVMGHLDKCFVSCTPYGILELLGRYNIETKGK
HCVVVGRSNIVGKPMANLMLQKLKESNCTVTICHSATPNITEFTKQADILIAAIGKANFITADMVKAGAVVIDVGINRVD
DASAKNGYRLVGDVEYAGVSALASAITPVPGGVGPMTIAMLLKNTVQSFQRVNGL
>Mature_295_residues
MIVIDGKKVSLELKNELRERVEALNAKCGKVPGLTVIIVGEDPASQVYVRNKAKSCKEIGMNSTVIELPADTTQEHLLKI
INDLNNDDTVHGILVQQPMPKQIDEFTITLAIDPAKDVDGFHPENLGRLVMGHLDKCFVSCTPYGILELLGRYNIETKGK
HCVVVGRSNIVGKPMANLMLQKLKESNCTVTICHSATPNITEFTKQADILIAAIGKANFITADMVKAGAVVIDVGINRVD
DASAKNGYRLVGDVEYAGVSALASAITPVPGGVGPMTIAMLLKNTVQSFQRVNGL

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI94721354, Length=298, Percent_Identity=48.6577181208054, Blast_Score=243, Evalue=1e-64,
Organism=Homo sapiens, GI222136639, Length=292, Percent_Identity=43.8356164383562, Blast_Score=241, Evalue=4e-64,
Organism=Homo sapiens, GI222418558, Length=297, Percent_Identity=43.7710437710438, Blast_Score=228, Evalue=4e-60,
Organism=Homo sapiens, GI36796743, Length=202, Percent_Identity=29.7029702970297, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1786741, Length=286, Percent_Identity=46.5034965034965, Blast_Score=258, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI17568735, Length=264, Percent_Identity=45.0757575757576, Blast_Score=205, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6319558, Length=290, Percent_Identity=46.551724137931, Blast_Score=264, Evalue=1e-71,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=41.2751677852349, Blast_Score=224, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6322933, Length=311, Percent_Identity=26.3665594855305, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI62472483, Length=292, Percent_Identity=48.972602739726, Blast_Score=261, Evalue=4e-70,
Organism=Drosophila melanogaster, GI45551871, Length=292, Percent_Identity=48.972602739726, Blast_Score=261, Evalue=4e-70,
Organism=Drosophila melanogaster, GI24645718, Length=292, Percent_Identity=48.972602739726, Blast_Score=261, Evalue=5e-70,
Organism=Drosophila melanogaster, GI17137370, Length=292, Percent_Identity=48.972602739726, Blast_Score=261, Evalue=5e-70,
Organism=Drosophila melanogaster, GI17136818, Length=295, Percent_Identity=47.7966101694915, Blast_Score=258, Evalue=4e-69,
Organism=Drosophila melanogaster, GI17136816, Length=295, Percent_Identity=47.7966101694915, Blast_Score=258, Evalue=4e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_CHLCH (Q3ASI0)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_379088.1
- ProteinModelPortal:   Q3ASI0
- SMR:   Q3ASI0
- STRING:   Q3ASI0
- GeneID:   3748064
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_0779
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   CLMMLRD
- ProtClustDB:   PRK14174
- BioCyc:   CCHL340177:CAG_0779-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31744; Mature: 31744

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVIDGKKVSLELKNELRERVEALNAKCGKVPGLTVIIVGEDPASQVYVRNKAKSCKEIG
CEEECCCEEEEHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEHHHHHHHHHCC
MNSTVIELPADTTQEHLLKIINDLNNDDTVHGILVQQPMPKQIDEFTITLAIDPAKDVDG
CCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCC
FHPENLGRLVMGHLDKCFVSCTPYGILELLGRYNIETKGKHCVVVGRSNIVGKPMANLML
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHH
QKLKESNCTVTICHSATPNITEFTKQADILIAAIGKANFITADMVKAGAVVIDVGINRVD
HHHHCCCCEEEEEECCCCCHHHHHHHHCEEEEEECCCCEEEHHHHHCCEEEEEECCCCCC
DASAKNGYRLVGDVEYAGVSALASAITPVPGGVGPMTIAMLLKNTVQSFQRVNGL
CCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIVIDGKKVSLELKNELRERVEALNAKCGKVPGLTVIIVGEDPASQVYVRNKAKSCKEIG
CEEECCCEEEEHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEHHHHHHHHHCC
MNSTVIELPADTTQEHLLKIINDLNNDDTVHGILVQQPMPKQIDEFTITLAIDPAKDVDG
CCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCCC
FHPENLGRLVMGHLDKCFVSCTPYGILELLGRYNIETKGKHCVVVGRSNIVGKPMANLML
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHH
QKLKESNCTVTICHSATPNITEFTKQADILIAAIGKANFITADMVKAGAVVIDVGINRVD
HHHHCCCCEEEEEECCCCCHHHHHHHHCEEEEEECCCCEEEHHHHHCCEEEEEECCCCCC
DASAKNGYRLVGDVEYAGVSALASAITPVPGGVGPMTIAMLLKNTVQSFQRVNGL
CCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA