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The map label for this gene is radA [H]

Identifier: 78188749

GI number: 78188749

Start: 1096584

End: 1097948

Strand: Reverse

Name: radA [H]

Synonym: Cag_0778

Alternate gene names: 78188749

Gene position: 1097948-1096584 (Counterclockwise)

Preceding gene: 78188750

Following gene: 78188737

Centisome position: 42.69

GC content: 49.23

Gene sequence:

>1365_bases
ATGGCAAAAGCTACTACCCGCTACGTTTGCTCCAACTGTGGCGCTGTTTCTCTTAAATATCAAGGGAAATGTTTTGAGTG
CCAAAGTTGGGGCACCTTGCAGGAAATGCGTATTGAACCCGAACCCAAGCACTCACAGCGTGGCATACCCGAAAAAAGTG
CCACCGTGCAGCGCCTGCATGACGTTGTGCCATCGGAATTTCAACGCATTACCACTGGTATGGGGGAACTTGATCGCGTG
CTGGGTGGTGGCTTAATGCAAGCATCGGCTGTGTTAGTGGGTGGAGAGCCAGGCATTGGTAAATCAACGCTGATGCTCCA
TCTTGCCCCTCGCCTTGCCCCCGCAAAAGTGCTCTACATTTCGGGCGAAGAGTCCCCCAACCAAATTCGAGAACGCGCCG
AGCGCCTTGCCATTCGTGCCGATAACCTTTGGTTAGTTCCTGAAGTAAATCTTGAACGCATTCTTTCGCTTTTAGAGCAC
GAAAAACCAGCACTTGTTATTATTGACTCCATTCAAACCATCCACTCCAGCGACTACCAAAGTGCGGCAGGCACCATTAC
ACAAATTCGCGAATGTGCCGCAGCGCTTATTCGCACCGCCAAAGCGCACAATATTATTTTGATGATTATTGGGCACATCA
CAAAAGAGGGCGCCCTTGCAGGACCAAAAGCACTTGAGCATATGGTTGACACCGTGCTACAGTTTGAGGGAGAAAATTAT
CAGCGCTACCGAATTATTCGCTCAGTAAAAAACCGCTTTGGCTCCACCAACGAAATTGGTGTTTTCCGCATGGATGAAAC
AGGGCTTGAAGAGGTAAGCAATCCATCGGAATTTTTTATTGCAGGTAGAAATAGCGATGTACCGGGGAATGCCATTCTTG
CCGCACTTGAAGGCTCACGCGCACTCTTAGTGGAAGTACAAGCGCTGGTAGCAAAAACCAACTACTCCATGCCCCAGCGC
ATTAGCACAGGCTTCGACCTCAAGCGTATCTCCATTATTTTAGCGGTGTTAGAAAAACGCTTACTGATTGAAACGTGGGG
ACAGGATGTTTTTGTAAAAATTGCAGGCGGCTTAAAATTAGTAGAACCTGCCGCTGACCTTGCTATTGCTGCCGCCATAG
CTTCAGGCTTAATGAATCGCACGATTGACGCTGGCACGGTGTGTTGTGGCGAAATTGGATTAGCGGGCGAACTTCGTGCC
ATTAGCGATAGCGAACGGCGCATTCGCGAAGCTGCTCATCTTGGTTTTCAGCGCATTGTGCTTCCTGCAGCCAACACCCG
TGAACTTAAAGCATCGCTTAAAAAGCTGCCCATCTCAATTATTGGTTGCGCCACATTGCATCAAGCGCTTGATGAGATGG
TGTAG

Upstream 100 bases:

>100_bases
CCAGGTGGCGTTGGTCCTATGACTATTGCCATGCTACTTAAAAACACCGTGCAATCGTTCCAACGCGTGAACGGACTATA
AAAAGGCGCGATAACTCACT

Downstream 100 bases:

>100_bases
GAAGCTATCTTTTAGCTTTACCGTTACTTTTTATAACAAATAAATAATAGCAATCAATAATGAGAGAAATAGATACAATA
GTAAATCAGTTAGAAAAACA

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MAKATTRYVCSNCGAVSLKYQGKCFECQSWGTLQEMRIEPEPKHSQRGIPEKSATVQRLHDVVPSEFQRITTGMGELDRV
LGGGLMQASAVLVGGEPGIGKSTLMLHLAPRLAPAKVLYISGEESPNQIRERAERLAIRADNLWLVPEVNLERILSLLEH
EKPALVIIDSIQTIHSSDYQSAAGTITQIRECAAALIRTAKAHNIILMIIGHITKEGALAGPKALEHMVDTVLQFEGENY
QRYRIIRSVKNRFGSTNEIGVFRMDETGLEEVSNPSEFFIAGRNSDVPGNAILAALEGSRALLVEVQALVAKTNYSMPQR
ISTGFDLKRISIILAVLEKRLLIETWGQDVFVKIAGGLKLVEPAADLAIAAAIASGLMNRTIDAGTVCCGEIGLAGELRA
ISDSERRIREAAHLGFQRIVLPAANTRELKASLKKLPISIIGCATLHQALDEMV

Sequences:

>Translated_454_residues
MAKATTRYVCSNCGAVSLKYQGKCFECQSWGTLQEMRIEPEPKHSQRGIPEKSATVQRLHDVVPSEFQRITTGMGELDRV
LGGGLMQASAVLVGGEPGIGKSTLMLHLAPRLAPAKVLYISGEESPNQIRERAERLAIRADNLWLVPEVNLERILSLLEH
EKPALVIIDSIQTIHSSDYQSAAGTITQIRECAAALIRTAKAHNIILMIIGHITKEGALAGPKALEHMVDTVLQFEGENY
QRYRIIRSVKNRFGSTNEIGVFRMDETGLEEVSNPSEFFIAGRNSDVPGNAILAALEGSRALLVEVQALVAKTNYSMPQR
ISTGFDLKRISIILAVLEKRLLIETWGQDVFVKIAGGLKLVEPAADLAIAAAIASGLMNRTIDAGTVCCGEIGLAGELRA
ISDSERRIREAAHLGFQRIVLPAANTRELKASLKKLPISIIGCATLHQALDEMV
>Mature_453_residues
AKATTRYVCSNCGAVSLKYQGKCFECQSWGTLQEMRIEPEPKHSQRGIPEKSATVQRLHDVVPSEFQRITTGMGELDRVL
GGGLMQASAVLVGGEPGIGKSTLMLHLAPRLAPAKVLYISGEESPNQIRERAERLAIRADNLWLVPEVNLERILSLLEHE
KPALVIIDSIQTIHSSDYQSAAGTITQIRECAAALIRTAKAHNIILMIIGHITKEGALAGPKALEHMVDTVLQFEGENYQ
RYRIIRSVKNRFGSTNEIGVFRMDETGLEEVSNPSEFFIAGRNSDVPGNAILAALEGSRALLVEVQALVAKTNYSMPQRI
STGFDLKRISIILAVLEKRLLIETWGQDVFVKIAGGLKLVEPAADLAIAAAIASGLMNRTIDAGTVCCGEIGLAGELRAI
SDSERRIREAAHLGFQRIVLPAANTRELKASLKKLPISIIGCATLHQALDEMV

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=460, Percent_Identity=43.4782608695652, Blast_Score=383, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49426; Mature: 49295

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKATTRYVCSNCGAVSLKYQGKCFECQSWGTLQEMRIEPEPKHSQRGIPEKSATVQRLH
CCCCHHHHHHCCCCEEEEEECCCEEEECCCCCHHHHCCCCCCCHHHCCCCCHHHHHHHHH
DVVPSEFQRITTGMGELDRVLGGGLMQASAVLVGGEPGIGKSTLMLHLAPRLAPAKVLYI
HHHHHHHHHHHHCHHHHHHHHCCCHHHEEEEEECCCCCCCCCEEEEEECCCCCCEEEEEE
SGEESPNQIRERAERLAIRADNLWLVPEVNLERILSLLEHEKPALVIIDSIQTIHSSDYQ
ECCCCHHHHHHHHHHHEEEECCEEEECCCCHHHHHHHHHCCCCEEEEEECHHHHHCCCHH
SAAGTITQIRECAAALIRTAKAHNIILMIIGHITKEGALAGPKALEHMVDTVLQFEGENY
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHCCCCCCCCHHHHHHHHHHHHHHCCCCH
QRYRIIRSVKNRFGSTNEIGVFRMDETGLEEVSNPSEFFIAGRNSDVPGNAILAALEGSR
HHHHHHHHHHHHCCCCCCEEEEEECCCCHHHCCCCCCEEEECCCCCCCCCCEEEEECCCC
ALLVEVQALVAKTNYSMPQRISTGFDLKRISIILAVLEKRLLIETWGQDVFVKIAGGLKL
EEHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEE
VEPAADLAIAAAIASGLMNRTIDAGTVCCGEIGLAGELRAISDSERRIREAAHLGFQRIV
ECHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHCCCHHHHHHHHHHCCHHEEE
LPAANTRELKASLKKLPISIIGCATLHQALDEMV
ECCCCHHHHHHHHHHCCHHEEHHHHHHHHHHHCC
>Mature Secondary Structure 
AKATTRYVCSNCGAVSLKYQGKCFECQSWGTLQEMRIEPEPKHSQRGIPEKSATVQRLH
CCCHHHHHHCCCCEEEEEECCCEEEECCCCCHHHHCCCCCCCHHHCCCCCHHHHHHHHH
DVVPSEFQRITTGMGELDRVLGGGLMQASAVLVGGEPGIGKSTLMLHLAPRLAPAKVLYI
HHHHHHHHHHHHCHHHHHHHHCCCHHHEEEEEECCCCCCCCCEEEEEECCCCCCEEEEEE
SGEESPNQIRERAERLAIRADNLWLVPEVNLERILSLLEHEKPALVIIDSIQTIHSSDYQ
ECCCCHHHHHHHHHHHEEEECCEEEECCCCHHHHHHHHHCCCCEEEEEECHHHHHCCCHH
SAAGTITQIRECAAALIRTAKAHNIILMIIGHITKEGALAGPKALEHMVDTVLQFEGENY
HHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHCCCCCCCCHHHHHHHHHHHHHHCCCCH
QRYRIIRSVKNRFGSTNEIGVFRMDETGLEEVSNPSEFFIAGRNSDVPGNAILAALEGSR
HHHHHHHHHHHHCCCCCCEEEEEECCCCHHHCCCCCCEEEECCCCCCCCCCEEEEECCCC
ALLVEVQALVAKTNYSMPQRISTGFDLKRISIILAVLEKRLLIETWGQDVFVKIAGGLKL
EEHHHHHHHHHHCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCEE
VEPAADLAIAAAIASGLMNRTIDAGTVCCGEIGLAGELRAISDSERRIREAAHLGFQRIV
ECHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCHHHCCCHHHHHHHHHHCCHHEEE
LPAANTRELKASLKKLPISIIGCATLHQALDEMV
ECCCCHHHHHHHHHHCCHHEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]