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The map label for this gene is dnaG [H]

Identifier: 78188526

GI number: 78188526

Start: 652586

End: 654568

Strand: Reverse

Name: dnaG [H]

Synonym: Cag_0548

Alternate gene names: 78188526

Gene position: 654568-652586 (Counterclockwise)

Preceding gene: 78188531

Following gene: 78188525

Centisome position: 25.45

GC content: 48.61

Gene sequence:

>1983_bases
ATGAGCATGATTCCGCCCGCAATTATTGATGAGGTTCGCCAAGCTGCCGACATTGTTGATGTTGTTTCGGATTATGTTGC
CCTGCAACCTTCGGGGCGTAACTATAAAGCCCTTTCTCCCTTTACGCAAGAAAAAACTCCCTCCTTTATTGTTTCCCCCG
ATAAGCAAATTTACAAGTGCTTTTCAACGGGCAAAGCGGGCAATGTTTTTTCTTTTATTATGGAGATGGAAAAAGTCCCC
TTTATGGAGGCGCTGAAGTTAGTAGCGCAACGGGCGGGGATTGATATTAGCCGCTATACGGAGCCAAAAGGAAAGCAGGA
AGGAGAGGAGGAGCAAGGAAGTGGGGCGGCTTTGCGTTGGGCAGCTCGCATGTTTCATAGTTTGCTGAAGCAACCCGCTG
GTGCTGAAGGATGGCGTTATTTTGTGGAAGAGCGGGGATTGCGGGAGGAAACCATCAACCGTTTTGGTTTGGGCTATGCG
CCTGAAAGCTGGGATTTTTTATTGCGTGAAGCAAGGCGCGAGGGTATTAAGAGCGAGCAGTTGGTTGAACTTGGGTTGCT
TGTTAGCCATCGCGAAAAACAGTCGCTGTATGACAGCTTTCGCCACCGAGTAATATTCCCCATATTTTCACGCGGTGGGC
AGGTGGTTGGCTTCGGTGGTCGGGCGTTAGTAAGCGATGAGCGTTCGCCGAAGTACCTCAATTCGCCCGAAAGCGCCATG
TTTGCAAAGTCGAAGCTCCTCTATGGCTTACATTTTGCAAAAAATGAAATTCGCCGCCAAGAGCGCGCTATTCTTGTAGA
AGGGTATATGGATGTTTTAGCGCTGCATCAAGCAGGGCTTACCAATGCCGTTGCCTCGTGCGGTACAGCGCTGACGCGCT
ATCAAGCAAAAATGTTGCGCCACTATAGCGAGCATGTGCTCTTTGTTTATGATGCCGATAAAGCAGGGCAGAAGTCTATG
ATGAGCGGCATTGATATTCTTGTGAGCGAGCAAATGGTGCCTCAAGTGCTTATGCTGCCCGAAGGCGACGACCCCGATAG
CTTTGTGCGGCGCGAAGGACGACAGGGATTTTTGCAATACGCCGAAAGCCACACTATGGGCTTTCAAGATTTTCAGCTTG
CTTTTTTTGAAGCCGCAGGAGCATTTTCTACCCCTGAGCAAAAAGCTGAAGCGCTTCGAGTGATGGTACGAACCATTGCG
CTCATCCCTAAACGTGCGCAACGGGAATTGTATGCGCAAGAGCTTTCAAAAAAAGTAGGGTTAACCGTTACAGCACTTCG
AGAGTTGTTGGGAAATGCTACCAGCGCTGTTGCCAAGCAACAATCGTGCACGCCGAGCAAAGCCTCCGCAACAGCACCCA
CATCCTCGTCCGCCACAAATGCTACAAGCGCTCCAACTATTCCACATGCTCCCAATCTCCCTAATGCCCAAGCGCTGCCA
CCACTCTCCGTGCTTGAAAAAACCTTTCTTAAAGCTCTGCTTGAAAGCACGCAATATGGTACTGCCGTACTGGGTTTTGC
AGCCTCGCATCAAAGTATGTTAGAGCTTCGTCATCCCTTAGCGCAAGAAATTTTTGCTCACCTTATCCACCGCTACCACA
ACATAGCCGCCGACCCAGAAGCCACTATTGATATGGTTTCTGAAATTAGCGCATTCACCAACCCCGAAACACGCGATCTT
GCCTCTACCCTTTTGCTTGATCCTCCCATTAGCCCCAAATGGCAACAACAAAACGATCTGTTTAGTGAACAAGCACGCCG
CTGTCTTGCTATGTTTCTTGACGCATTTAAGAATTTAGTGTTGGAGCCATTGCTTGACGAAAAAAACAAGCTTATGGAGC
AAATTCGTGTTGAGGAAAATGTGGAGCGTGAAATTGAGCTATCGAGGCAAAAAATTGTGCTCGATAAAAAAATTCGTGAA
GAGAACCGCAGCTTACAGCAAATGATTAAGGCAATTCTTGATTCAACGCAGCAGGTTGGGTAG

Upstream 100 bases:

>100_bases
ATGTGTTCCGTGATGCTGCTAATGTAAGCAAATGCTTACATTGTCATCTTTCTTCTAAACGCATTTTCAATCCCCAATTT
TTCTCCAATACGAAAAGCGC

Downstream 100 bases:

>100_bases
GAAGTGGGCAATGGTATGCCCTTGCCTTCATCTATAAAAAATTCGTACTATGACACCCTTGAAAAGTCAACCGAACCATT
CAATTCCACAAGCTGAACTT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 660; Mature: 659

Protein sequence:

>660_residues
MSMIPPAIIDEVRQAADIVDVVSDYVALQPSGRNYKALSPFTQEKTPSFIVSPDKQIYKCFSTGKAGNVFSFIMEMEKVP
FMEALKLVAQRAGIDISRYTEPKGKQEGEEEQGSGAALRWAARMFHSLLKQPAGAEGWRYFVEERGLREETINRFGLGYA
PESWDFLLREARREGIKSEQLVELGLLVSHREKQSLYDSFRHRVIFPIFSRGGQVVGFGGRALVSDERSPKYLNSPESAM
FAKSKLLYGLHFAKNEIRRQERAILVEGYMDVLALHQAGLTNAVASCGTALTRYQAKMLRHYSEHVLFVYDADKAGQKSM
MSGIDILVSEQMVPQVLMLPEGDDPDSFVRREGRQGFLQYAESHTMGFQDFQLAFFEAAGAFSTPEQKAEALRVMVRTIA
LIPKRAQRELYAQELSKKVGLTVTALRELLGNATSAVAKQQSCTPSKASATAPTSSSATNATSAPTIPHAPNLPNAQALP
PLSVLEKTFLKALLESTQYGTAVLGFAASHQSMLELRHPLAQEIFAHLIHRYHNIAADPEATIDMVSEISAFTNPETRDL
ASTLLLDPPISPKWQQQNDLFSEQARRCLAMFLDAFKNLVLEPLLDEKNKLMEQIRVEENVEREIELSRQKIVLDKKIRE
ENRSLQQMIKAILDSTQQVG

Sequences:

>Translated_660_residues
MSMIPPAIIDEVRQAADIVDVVSDYVALQPSGRNYKALSPFTQEKTPSFIVSPDKQIYKCFSTGKAGNVFSFIMEMEKVP
FMEALKLVAQRAGIDISRYTEPKGKQEGEEEQGSGAALRWAARMFHSLLKQPAGAEGWRYFVEERGLREETINRFGLGYA
PESWDFLLREARREGIKSEQLVELGLLVSHREKQSLYDSFRHRVIFPIFSRGGQVVGFGGRALVSDERSPKYLNSPESAM
FAKSKLLYGLHFAKNEIRRQERAILVEGYMDVLALHQAGLTNAVASCGTALTRYQAKMLRHYSEHVLFVYDADKAGQKSM
MSGIDILVSEQMVPQVLMLPEGDDPDSFVRREGRQGFLQYAESHTMGFQDFQLAFFEAAGAFSTPEQKAEALRVMVRTIA
LIPKRAQRELYAQELSKKVGLTVTALRELLGNATSAVAKQQSCTPSKASATAPTSSSATNATSAPTIPHAPNLPNAQALP
PLSVLEKTFLKALLESTQYGTAVLGFAASHQSMLELRHPLAQEIFAHLIHRYHNIAADPEATIDMVSEISAFTNPETRDL
ASTLLLDPPISPKWQQQNDLFSEQARRCLAMFLDAFKNLVLEPLLDEKNKLMEQIRVEENVEREIELSRQKIVLDKKIRE
ENRSLQQMIKAILDSTQQVG
>Mature_659_residues
SMIPPAIIDEVRQAADIVDVVSDYVALQPSGRNYKALSPFTQEKTPSFIVSPDKQIYKCFSTGKAGNVFSFIMEMEKVPF
MEALKLVAQRAGIDISRYTEPKGKQEGEEEQGSGAALRWAARMFHSLLKQPAGAEGWRYFVEERGLREETINRFGLGYAP
ESWDFLLREARREGIKSEQLVELGLLVSHREKQSLYDSFRHRVIFPIFSRGGQVVGFGGRALVSDERSPKYLNSPESAMF
AKSKLLYGLHFAKNEIRRQERAILVEGYMDVLALHQAGLTNAVASCGTALTRYQAKMLRHYSEHVLFVYDADKAGQKSMM
SGIDILVSEQMVPQVLMLPEGDDPDSFVRREGRQGFLQYAESHTMGFQDFQLAFFEAAGAFSTPEQKAEALRVMVRTIAL
IPKRAQRELYAQELSKKVGLTVTALRELLGNATSAVAKQQSCTPSKASATAPTSSSATNATSAPTIPHAPNLPNAQALPP
LSVLEKTFLKALLESTQYGTAVLGFAASHQSMLELRHPLAQEIFAHLIHRYHNIAADPEATIDMVSEISAFTNPETRDLA
STLLLDPPISPKWQQQNDLFSEQARRCLAMFLDAFKNLVLEPLLDEKNKLMEQIRVEENVEREIELSRQKIVLDKKIREE
NRSLQQMIKAILDSTQQVG

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=495, Percent_Identity=33.1313131313131, Blast_Score=258, Evalue=6e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 73821; Mature: 73690

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMIPPAIIDEVRQAADIVDVVSDYVALQPSGRNYKALSPFTQEKTPSFIVSPDKQIYKC
CCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCEEECCHHHHHHH
FSTGKAGNVFSFIMEMEKVPFMEALKLVAQRAGIDISRYTEPKGKQEGEEEQGSGAALRW
HHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHCCCCHHHHH
AARMFHSLLKQPAGAEGWRYFVEERGLREETINRFGLGYAPESWDFLLREARREGIKSEQ
HHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHH
LVELGLLVSHREKQSLYDSFRHRVIFPIFSRGGQVVGFGGRALVSDERSPKYLNSPESAM
HHHHHHHHHHHHHHHHHHHHHHHEEEHHHHCCCCEEEECCCEEECCCCCCCCCCCCHHHH
FAKSKLLYGLHFAKNEIRRQERAILVEGYMDVLALHQAGLTNAVASCGTALTRYQAKMLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HYSEHVLFVYDADKAGQKSMMSGIDILVSEQMVPQVLMLPEGDDPDSFVRREGRQGFLQY
HHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHH
AESHTMGFQDFQLAFFEAAGAFSTPEQKAEALRVMVRTIALIPKRAQRELYAQELSKKVG
HHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHC
LTVTALRELLGNATSAVAKQQSCTPSKASATAPTSSSATNATSAPTIPHAPNLPNAQALP
CHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PLSVLEKTFLKALLESTQYGTAVLGFAASHQSMLELRHPLAQEIFAHLIHRYHNIAADPE
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
ATIDMVSEISAFTNPETRDLASTLLLDPPISPKWQQQNDLFSEQARRCLAMFLDAFKNLV
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LEPLLDEKNKLMEQIRVEENVEREIELSRQKIVLDKKIREENRSLQQMIKAILDSTQQVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SMIPPAIIDEVRQAADIVDVVSDYVALQPSGRNYKALSPFTQEKTPSFIVSPDKQIYKC
CCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCEEECCHHHHHHH
FSTGKAGNVFSFIMEMEKVPFMEALKLVAQRAGIDISRYTEPKGKQEGEEEQGSGAALRW
HHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCCHHCCCCHHHHH
AARMFHSLLKQPAGAEGWRYFVEERGLREETINRFGLGYAPESWDFLLREARREGIKSEQ
HHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHH
LVELGLLVSHREKQSLYDSFRHRVIFPIFSRGGQVVGFGGRALVSDERSPKYLNSPESAM
HHHHHHHHHHHHHHHHHHHHHHHEEEHHHHCCCCEEEECCCEEECCCCCCCCCCCCHHHH
FAKSKLLYGLHFAKNEIRRQERAILVEGYMDVLALHQAGLTNAVASCGTALTRYQAKMLR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HYSEHVLFVYDADKAGQKSMMSGIDILVSEQMVPQVLMLPEGDDPDSFVRREGRQGFLQY
HHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHH
AESHTMGFQDFQLAFFEAAGAFSTPEQKAEALRVMVRTIALIPKRAQRELYAQELSKKVG
HHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHC
LTVTALRELLGNATSAVAKQQSCTPSKASATAPTSSSATNATSAPTIPHAPNLPNAQALP
CHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PLSVLEKTFLKALLESTQYGTAVLGFAASHQSMLELRHPLAQEIFAHLIHRYHNIAADPE
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
ATIDMVSEISAFTNPETRDLASTLLLDPPISPKWQQQNDLFSEQARRCLAMFLDAFKNLV
HHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LEPLLDEKNKLMEQIRVEENVEREIELSRQKIVLDKKIREENRSLQQMIKAILDSTQQVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961408; 11466286 [H]