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The map label for this gene is ruvC

Identifier: 78188147

GI number: 78188147

Start: 185205

End: 185783

Strand: Reverse

Name: ruvC

Synonym: Cag_0164

Alternate gene names: 78188147

Gene position: 185783-185205 (Counterclockwise)

Preceding gene: 162319775

Following gene: 78188146

Centisome position: 7.22

GC content: 51.12

Gene sequence:

>579_bases
ATGATTGTGCTTGGGGTTGATCCCGGTAGTTTAAAAACAGGTTATGGTGTGGTGCAGCACCATAACGGCTCTTTTTCGGT
TTTGGCGGCTGGAGTTATTCGCTTGCAAGCTGCGTGGAGCCATCCCGAGCGCATTGGCATTATTTGCCGTGAGCTTGAGC
AAGTTATTGCAGAATTCCAGCCAGAGCGGGTGGCGCTTGAAACAGCTTTTTTAAGCCACAACGTACAAGCCGCTTTAAAG
CTTGGGCAGGTGCGTGGTGCCGTTATTGGTTTGGTGGTACGTTATGCCCTGCCGATTTATGAATATGCGCCTCGTGAAGT
AAAGTCGGCTATTACAGGCAAAGGAGCAGCAACAAAAGAGCAAGTTGCCTTTATGGTAAGCCGAATGCTTTCGCTTCATA
CGGTGCCAAAGCCGCACGATGTTACCGATGCTTTAGGAATTGCTCTTTGCGATATTTTGCGGGGTGAAAGTCGCCAAAGC
GGAGTGCCACCACGTACCAACAGTCGCCGCAAAAGCGGGACGGGGGGAAGTTGGGAACAATTTGTGCGGCAGTCGCCCAA
CGTTGTGGTTCGCTCGTAA

Upstream 100 bases:

>100_bases
TGATTGATGCGCTTGAAAGTAATGACGATGTACAAGCTGTTTATAGCAACATGGAGCTAAGTGAAAGCGCCATGAATAGC
TTGAGCGAGTAAGTTTCTTT

Downstream 100 bases:

>100_bases
CGCTTCCGTTTATTGTTTTTTTGTTTCGACCTCATTATTGGTAGTTTTCTTGATGAGTTGCATCAAAAACTACCTCTTAA
CTGTTCCAACCATTTCTATG

Product: crossover junction endodeoxyribonuclease RuvC

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MIVLGVDPGSLKTGYGVVQHHNGSFSVLAAGVIRLQAAWSHPERIGIICRELEQVIAEFQPERVALETAFLSHNVQAALK
LGQVRGAVIGLVVRYALPIYEYAPREVKSAITGKGAATKEQVAFMVSRMLSLHTVPKPHDVTDALGIALCDILRGESRQS
GVPPRTNSRRKSGTGGSWEQFVRQSPNVVVRS

Sequences:

>Translated_192_residues
MIVLGVDPGSLKTGYGVVQHHNGSFSVLAAGVIRLQAAWSHPERIGIICRELEQVIAEFQPERVALETAFLSHNVQAALK
LGQVRGAVIGLVVRYALPIYEYAPREVKSAITGKGAATKEQVAFMVSRMLSLHTVPKPHDVTDALGIALCDILRGESRQS
GVPPRTNSRRKSGTGGSWEQFVRQSPNVVVRS
>Mature_192_residues
MIVLGVDPGSLKTGYGVVQHHNGSFSVLAAGVIRLQAAWSHPERIGIICRELEQVIAEFQPERVALETAFLSHNVQAALK
LGQVRGAVIGLVVRYALPIYEYAPREVKSAITGKGAATKEQVAFMVSRMLSLHTVPKPHDVTDALGIALCDILRGESRQS
GVPPRTNSRRKSGTGGSWEQFVRQSPNVVVRS

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=136, Percent_Identity=41.9117647058824, Blast_Score=110, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_CHLCH (Q3AU83)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_378485.1
- ProteinModelPortal:   Q3AU83
- SMR:   Q3AU83
- STRING:   Q3AU83
- GeneID:   3747729
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_0164
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- ProtClustDB:   CLSK637877
- BioCyc:   CCHL340177:CAG_0164-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176
- PRINTS:   PR00696
- TIGRFAMs:   TIGR00228

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 20788; Mature: 20788

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVLGVDPGSLKTGYGVVQHHNGSFSVLAAGVIRLQAAWSHPERIGIICRELEQVIAEFQ
CEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
PERVALETAFLSHNVQAALKLGQVRGAVIGLVVRYALPIYEYAPREVKSAITGKGAATKE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHH
QVAFMVSRMLSLHTVPKPHDVTDALGIALCDILRGESRQSGVPPRTNSRRKSGTGGSWEQ
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHH
FVRQSPNVVVRS
HHHCCCCEEEEC
>Mature Secondary Structure
MIVLGVDPGSLKTGYGVVQHHNGSFSVLAAGVIRLQAAWSHPERIGIICRELEQVIAEFQ
CEEEECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
PERVALETAFLSHNVQAALKLGQVRGAVIGLVVRYALPIYEYAPREVKSAITGKGAATKE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHH
QVAFMVSRMLSLHTVPKPHDVTDALGIALCDILRGESRQSGVPPRTNSRRKSGTGGSWEQ
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHH
FVRQSPNVVVRS
HHHCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA