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The map label for this gene is pgk

Identifier: 78188028

GI number: 78188028

Start: 49892

End: 51085

Strand: Reverse

Name: pgk

Synonym: Cag_0044

Alternate gene names: 78188028

Gene position: 51085-49892 (Counterclockwise)

Preceding gene: 78188029

Following gene: 78188027

Centisome position: 1.99

GC content: 48.91

Gene sequence:

>1194_bases
ATGGAAAAAAAAACCTTGTCTGACATAGCCACCCAAGGTAAACGGGTGCTGATGCGTGTTGATTTTAATGTACCGCTTGA
TGAAAACAAGAACATTACCGACGACAAGCGGATTGTTGAATCCCTCCCCTCCATCCGTAAAGTGATTGAAGAGGGCGGTC
GGTTGATTTTGATGTCGCACCTCGGACGCCCAAAAGGCAAGGTTAATGCAGAATTTTCGCTTGCACCCGTTGCTGCTCGG
CTGTCGGAACTTTTGGATTGCCCTGTTGGCATGGCAAAGGATTGCATTGGCACAGAGGTGATGCAGCAAGTGTTAGCCTT
GCAAGATGGTGAAGTGCTGCTGCTTGAAAATCTACGCTTTCATGCTGAAGAAGAGGCAAACGATGCCGATTTTGCAAAAG
AGCTTGCCTCACTTGGTGAAATTTATGTAAACGATGCCTTTGGTACCGCCCACCGCGCCCACGCATCAACCGAAGGTATT
ACCCACTACGTACAAACCGCCGTTGCAGGCTTTCTTATTGAGCGCGAACTACGCTACCTCGGCAAAGCCTTGCAAGAGCC
AGAACGCCCCTTTGTAGCTATTCTTGGTGGCTCAAAAATTTCAGGAAAAATTGATGTACTTGAAAACCTCTTTAAAAAGG
TTGATACCGTGCTCATTGGCGGCGCTATGGTCTTTACCTTCTTTAAAGCACAAGGCTACGAAGTAGGAAAATCGCTGGTT
GAAGAGAGCAAAATTGAGCTTGCCCTCTCCATTCTTGAACAAGCGAAAGCCAAAGGCATTAAGCTTCTGCTCCCCACCGA
CGTTGTTGTAACGGCTGAAATTTCCGCTGATGCAGAATCCTCAGTAGCCTCAATAGCCGATATGCCCAATAATTTAATTG
GGGTTGATATTGGACCCGAAACCGCCGCCGCCTACCGCAACGAAATTATTGGCGCTCGCACCGTATTGTGGAATGGACCT
ATGGGCGTTTTTGAACTCGACAACTTTGCAACAGGCACCATTGCCGTAGCACAAGCCCTTGCCGATGCAACCGCTCAAGG
CGCAACCACCATTGTTGGCGGTGGCGACTCTGCCGCAGCTATCGCAAAAGCAGGCTTAGCAAGCGAAATAACCCACATTT
CAACCGGAGGTGGTGCCAGCCTTGAATTCCTTGAAGGCAAAGAGCTACCCGGCATAGCAGCTCTTAACAACTAA

Upstream 100 bases:

>100_bases
ACTCTTCACTGAGCCTCCCACTCGTTGCTATACGTTTGAGAGAAACCTTACACACACAAGATGGAGAGTCGCTCATTTTT
ACAACCAACCACAACGAACA

Downstream 100 bases:

>100_bases
TAATTAATTGAACGCAATAGCAGAGAGAACGTATGCGCGATACACAACCTTTTCGCCCCGGGGGCTTCCAACTCATGCCA
CCGGCCATTAAAATCATTAT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MEKKTLSDIATQGKRVLMRVDFNVPLDENKNITDDKRIVESLPSIRKVIEEGGRLILMSHLGRPKGKVNAEFSLAPVAAR
LSELLDCPVGMAKDCIGTEVMQQVLALQDGEVLLLENLRFHAEEEANDADFAKELASLGEIYVNDAFGTAHRAHASTEGI
THYVQTAVAGFLIERELRYLGKALQEPERPFVAILGGSKISGKIDVLENLFKKVDTVLIGGAMVFTFFKAQGYEVGKSLV
EESKIELALSILEQAKAKGIKLLLPTDVVVTAEISADAESSVASIADMPNNLIGVDIGPETAAAYRNEIIGARTVLWNGP
MGVFELDNFATGTIAVAQALADATAQGATTIVGGGDSAAAIAKAGLASEITHISTGGGASLEFLEGKELPGIAALNN

Sequences:

>Translated_397_residues
MEKKTLSDIATQGKRVLMRVDFNVPLDENKNITDDKRIVESLPSIRKVIEEGGRLILMSHLGRPKGKVNAEFSLAPVAAR
LSELLDCPVGMAKDCIGTEVMQQVLALQDGEVLLLENLRFHAEEEANDADFAKELASLGEIYVNDAFGTAHRAHASTEGI
THYVQTAVAGFLIERELRYLGKALQEPERPFVAILGGSKISGKIDVLENLFKKVDTVLIGGAMVFTFFKAQGYEVGKSLV
EESKIELALSILEQAKAKGIKLLLPTDVVVTAEISADAESSVASIADMPNNLIGVDIGPETAAAYRNEIIGARTVLWNGP
MGVFELDNFATGTIAVAQALADATAQGATTIVGGGDSAAAIAKAGLASEITHISTGGGASLEFLEGKELPGIAALNN
>Mature_397_residues
MEKKTLSDIATQGKRVLMRVDFNVPLDENKNITDDKRIVESLPSIRKVIEEGGRLILMSHLGRPKGKVNAEFSLAPVAAR
LSELLDCPVGMAKDCIGTEVMQQVLALQDGEVLLLENLRFHAEEEANDADFAKELASLGEIYVNDAFGTAHRAHASTEGI
THYVQTAVAGFLIERELRYLGKALQEPERPFVAILGGSKISGKIDVLENLFKKVDTVLIGGAMVFTFFKAQGYEVGKSLV
EESKIELALSILEQAKAKGIKLLLPTDVVVTAEISADAESSVASIADMPNNLIGVDIGPETAAAYRNEIIGARTVLWNGP
MGVFELDNFATGTIAVAQALADATAQGATTIVGGGDSAAAIAKAGLASEITHISTGGGASLEFLEGKELPGIAALNN

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=414, Percent_Identity=43.9613526570048, Blast_Score=353, Evalue=2e-97,
Organism=Homo sapiens, GI31543397, Length=415, Percent_Identity=43.3734939759036, Blast_Score=347, Evalue=1e-95,
Organism=Escherichia coli, GI1789294, Length=392, Percent_Identity=44.6428571428571, Blast_Score=309, Evalue=2e-85,
Organism=Caenorhabditis elegans, GI17508823, Length=414, Percent_Identity=45.4106280193237, Blast_Score=359, Evalue=1e-99,
Organism=Saccharomyces cerevisiae, GI6319857, Length=411, Percent_Identity=46.7153284671533, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24581203, Length=416, Percent_Identity=45.4326923076923, Blast_Score=354, Evalue=5e-98,
Organism=Drosophila melanogaster, GI17136394, Length=415, Percent_Identity=44.0963855421687, Blast_Score=337, Evalue=9e-93,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_CHLCH (Q3ANU2)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_378366.1
- ProteinModelPortal:   Q3ANU2
- SMR:   Q3ANU2
- STRING:   Q3ANU2
- GeneID:   3747243
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_0044
- eggNOG:   COG0126
- HOGENOM:   HBG453500
- OMA:   NCAIILP
- ProtClustDB:   PRK00073
- BioCyc:   CCHL340177:CAG_0044-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 42083; Mature: 42083

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 37-37 BINDING 119-119 BINDING 152-152 BINDING 203-203 BINDING 294-294 BINDING 325-325

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKTLSDIATQGKRVLMRVDFNVPLDENKNITDDKRIVESLPSIRKVIEEGGRLILMSH
CCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEC
LGRPKGKVNAEFSLAPVAARLSELLDCPVGMAKDCIGTEVMQQVLALQDGEVLLLENLRF
CCCCCCCCCCEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
HAEEEANDADFAKELASLGEIYVNDAFGTAHRAHASTEGITHYVQTAVAGFLIERELRYL
CCCCCCCHHHHHHHHHHHHHEEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
GKALQEPERPFVAILGGSKISGKIDVLENLFKKVDTVLIGGAMVFTFFKAQGYEVGKSLV
HHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
EESKIELALSILEQAKAKGIKLLLPTDVVVTAEISADAESSVASIADMPNNLIGVDIGPE
HHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEEECCCCHHHHHHHHHCCCCEEEEECCCH
TAAAYRNEIIGARTVLWNGPMGVFELDNFATGTIAVAQALADATAQGATTIVGGGDSAAA
HHHHHHHHHHHHEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHH
IAKAGLASEITHISTGGGASLEFLEGKELPGIAALNN
HHHHHHHHHHHEEECCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MEKKTLSDIATQGKRVLMRVDFNVPLDENKNITDDKRIVESLPSIRKVIEEGGRLILMSH
CCCHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEC
LGRPKGKVNAEFSLAPVAARLSELLDCPVGMAKDCIGTEVMQQVLALQDGEVLLLENLRF
CCCCCCCCCCEEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
HAEEEANDADFAKELASLGEIYVNDAFGTAHRAHASTEGITHYVQTAVAGFLIERELRYL
CCCCCCCHHHHHHHHHHHHHEEEECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
GKALQEPERPFVAILGGSKISGKIDVLENLFKKVDTVLIGGAMVFTFFKAQGYEVGKSLV
HHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
EESKIELALSILEQAKAKGIKLLLPTDVVVTAEISADAESSVASIADMPNNLIGVDIGPE
HHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEEECCCCHHHHHHHHHCCCCEEEEECCCH
TAAAYRNEIIGARTVLWNGPMGVFELDNFATGTIAVAQALADATAQGATTIVGGGDSAAA
HHHHHHHHHHHHEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHH
IAKAGLASEITHISTGGGASLEFLEGKELPGIAALNN
HHHHHHHHHHHEEECCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA